LOCUS       NM_013485               2103 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus complement component 9 (C9), transcript variant 1,
            mRNA.
ACCESSION   NM_013485
VERSION     NM_013485.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Roy,T.A., Bubier,J.A., Dickson,P.E., Wilcox,T.D., Ndukum,J.,
            Clark,J.W., Sukoff Rizzo,S.J., Crabbe,J.C., Denegre,J.M.,
            Svenson,K.L., Braun,R.E., Kumar,V., Murray,S.A., White,J.K.,
            Philip,V.M. and Chesler,E.J.
  TITLE     Discovery and validation of genes driving drug-intake and related
            behavioral traits in mice
  JOURNAL   Genes Brain Behav 23 (1), e12875 (2024)
   PUBMED   38164795
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   3  (bases 1 to 2103)
  AUTHORS   Ueda,Y., Miwa,T., Ito,D., Kim,H., Sato,S., Gullipalli,D., Zhou,L.,
            Golla,M., Song,D., Dunaief,J.L., Palmer,M.B. and Song,W.C.
  TITLE     Differential contribution of C5aR and C5b-9 pathways to renal
            thrombic microangiopathy and macrovascular thrombosis in mice
            carrying an atypical hemolytic syndrome-related factor H mutation
  JOURNAL   Kidney Int 96 (1), 67-79 (2019)
   PUBMED   30910380
REFERENCE   4  (bases 1 to 2103)
  AUTHORS   Menny,A., Serna,M., Boyd,C.M., Gardner,S., Joseph,A.P.,
            Morgan,B.P., Topf,M., Brooks,N.J. and Bubeck,D.
  TITLE     CryoEM reveals how the complement membrane attack complex ruptures
            lipid bilayers
  JOURNAL   Nat Commun 9 (1), 5316 (2018)
   PUBMED   30552328
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2103)
  AUTHORS   Qiu,C., Huang,S., Park,J., Park,Y., Ko,Y.A., Seasock,M.J.,
            Bryer,J.S., Xu,X.X., Song,W.C., Palmer,M., Hill,J., Guarnieri,P.,
            Hawkins,J., Boustany-Kari,C.M., Pullen,S.S., Brown,C.D. and
            Susztak,K.
  TITLE     Renal compartment-specific genetic variation analyses identify new
            pathways in chronic kidney disease
  JOURNAL   Nat Med 24 (11), 1721-1731 (2018)
   PUBMED   30275566
REFERENCE   6  (bases 1 to 2103)
  AUTHORS   Fu,X., Ju,J., Lin,Z., Xiao,W., Li,X., Zhuang,B., Zhang,T., Ma,X.,
            Li,X., Ma,C., Su,W., Wang,Y., Qin,X. and Liang,S.
  TITLE     Target deletion of complement component 9 attenuates
            antibody-mediated hemolysis and lipopolysaccharide (LPS)-induced
            acute shock in mice
  JOURNAL   Sci Rep 6, 30239 (2016)
   PUBMED   27444648
  REMARK    GeneRIF: these results not only confirm the critical role of C5b-9
            in complement-mediated hemolysis and but also highlight the
            critical role of C5b-9 in inflammasome activation.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2103)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 2103)
  AUTHORS   Ghesquiere,B., Van Damme,J., Martens,L., Vandekerckhove,J. and
            Gevaert,K.
  TITLE     Proteome-wide characterization of N-glycosylation events by
            diagonal chromatography
  JOURNAL   J Proteome Res 5 (9), 2438-2447 (2006)
   PUBMED   16944957
REFERENCE   9  (bases 1 to 2103)
  AUTHORS   Caetano,A.R., Lyons,L.A., Laughlin,T.F., O'Brien,S.J., Murray,J.D.
            and Bowling,A.T.
  TITLE     Equine synteny mapping of comparative anchor tagged sequences
            (CATS) from human Chromosome 5
  JOURNAL   Mamm Genome 10 (11), 1082-1084 (1999)
   PUBMED   10556427
REFERENCE   10 (bases 1 to 2103)
  AUTHORS   Stanley,K.K. and Herz,J.
  TITLE     Topological mapping of complement component C9 by recombinant DNA
            techniques suggests a novel mechanism for its insertion into target
            membranes
  JOURNAL   EMBO J 6 (7), 1951-1957 (1987)
   PUBMED   2443347
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC105324.11 and AC161196.4.
            
            On Feb 13, 2019 this sequence version replaced NM_013485.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011137.1, X05475.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-138               AC105324.11        24947-25084         c
            139-232             AC105324.11        12160-12253         c
            233-377             AC105324.11        11403-11547         c
            378-525             AC105324.11        10758-10905         c
            526-658             AC105324.11        2965-3097           c
            659-919             AC161196.4         140086-140346       c
            920-1160            AC161196.4         136526-136766       c
            1161-1301           AC161196.4         133419-133559       c
            1302-1480           AC161196.4         131848-132026       c
            1481-1709           AC161196.4         126208-126436       c
            1710-2103           AC161196.4         124562-124955       c
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 2.79 cM"
     gene            1..2103
                     /gene="C9"
                     /note="complement component 9"
                     /db_xref="GeneID:12279"
                     /db_xref="MGI:MGI:1098282"
     exon            1..138
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..1702
                     /gene="C9"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; complement component C9"
                     /codon_start=1
                     /product="complement component C9 isoform 1 precursor"
                     /protein_id="NP_038513.2"
                     /db_xref="CCDS:CCDS27367.1"
                     /db_xref="GeneID:12279"
                     /db_xref="MGI:MGI:1098282"
                     /translation="MASGMAITLALAIFALGVNAQMPIPVSREEQEQHYPIPIDCRMS
                     PWSNWSECDPCLKQRFRSRSILAFGQFNGKSCVDVLGDRQGCEPTQECEEIQENCGND
                     FQCETGRCIKRRLLCNGDNDCGDYSDENDCDDDPRTPCRDRVAEESELGLTAGYGINI
                     LGMEPLRTPFDNEFYNGLCDRVRDEKTYYRKPWNVVSLIYETKADKSFRTENYDEHLE
                     VFKAINREKTSNFNADFALKFSATEVPEKGAGEVSPAEHSSKPTNISAKFKFSYFMGK
                     NFRRLSSYFSQSKKMFVHLRGVVQLGRFVMRNRDVVLRSTFLDDVKALPTSYEKGEYF
                     GFLETYGTHYSTSGSLGGQYEIVYVLDKASMKEKGVDLNDVKHCLGFNMDLRIPLQDD
                     LKDASVTASVNADGCIKTDNGKTVNITRDNIIDDVISFIRGGTREQAILLKEKILRGD
                     KTFDKTDFANWASSLANAPALISQRMSPIYNLIPLKIKDAYIKKQNLEKAVEDYIDEF
                     STKRCYPCLNGGTIILLDGQCLCSCPMMFRGMACEIHQKI"
     exon            139..232
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            233..377
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            378..525
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            526..658
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            659..919
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1160
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1301
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1480
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1709
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..2103
                     /gene="C9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2009..2014
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C9"
                     /note="hexamer: AACAAA"
     regulatory      2015..2020
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C9"
                     /note="hexamer: ATTAAA"
     regulatory      2020..2025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C9"
                     /note="hexamer: ATTAAA"
     polyA_site      2029
                     /gene="C9"
                     /note="major polyA site"
     polyA_site      2032
                     /gene="C9"
     polyA_site      2045
                     /gene="C9"
     regulatory      2081..2086
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C9"
                     /note="hexamer: AATATA"
     polyA_site      2103
                     /gene="C9"
ORIGIN      
        1 ctccggctgc aaaggaatgc tttgcaagcc tccagggctc cccaggagga gcagcatggc
       61 ctcaggcatg gccatcacct tagcccttgc catctttgcc ttgggtgtca atgcacagat
      121 gccaataccc gtttccagag aagaacaaga acaacactat cccataccga tagactgcag
      181 aatgagccca tggagcaatt ggtcagagtg tgatccttgc ctcaaacaaa ggtttcgctc
      241 aagaagcatt ttagccttcg gacagtttaa tgggaaaagc tgtgttgatg ttttgggaga
      301 cagacaaggc tgtgaaccca cccaggagtg tgaagagata caggaaaact gtggaaatga
      361 ctttcagtgt gagacaggca ggtgcataaa gaggagactt ctgtgtaatg gtgacaacga
      421 ctgtggagat tattctgatg agaatgactg tgacgatgac ccacgcaccc catgccgtga
      481 ccgagtagcg gaagaatcag agctgggact aacagcaggc tatgggatca acatcttagg
      541 gatggagccc ctgagaacac cttttgacaa tgagttctac aacggactct gtgaccgggt
      601 acgagacgaa aagacatact atcgcaaacc ttggaatgta gtttctctga tctatgaaac
      661 caaggctgat aaaagtttca gaactgagaa ctatgacgaa cacttggaag tattcaaagc
      721 catcaaccga gagaagacct cgaattttaa tgcagatttt gccctaaaat tttcagccac
      781 cgaagtacct gaaaagggag ctggggaagt ctccccagca gaacactctt caaaacctac
      841 aaacatttca gctaaattta aattttcata tttcatggga aaaaattttc gaagactatc
      901 atcttatttt tcgcagtcga aaaagatgtt tgtgcacttg agaggagtgg tccaactggg
      961 gagatttgta atgaggaatc gggatgttgt gctgaggtca actttcctgg atgatgtaaa
     1021 agctctacca acttcctatg aaaagggaga atattttgga tttttggaaa cctatgggac
     1081 tcactacagt acctctgggt ccctgggagg acaatatgaa attgtctatg tcttggataa
     1141 agcttccatg aaagagaaag gtgttgacct gaatgatgta aaacattgtc ttggatttaa
     1201 tatggattta cgtattcctc tacaagacga cttaaaggat gcatcagtca cagcaagtgt
     1261 taatgcggat ggttgcataa agacagataa tgggaaaact gtaaacatca cccgcgataa
     1321 catcatagat gatgtcattt cattcataag aggagggact agggagcaag caattctcct
     1381 gaaagagaag attctcagag gagacaagac atttgataag actgacttcg ccaactgggc
     1441 ctcgtccctg gcaaacgctc cagctctcat cagtcaaaga atgtccccta tatataatct
     1501 cattcctttg aaaataaaag atgcatacat aaagaagcaa aatttggaaa aggctgttga
     1561 agactatata gatgaattca gtactaaaag gtgctaccca tgtctaaatg gaggtactat
     1621 aattcttctg gatgggcagt gcctgtgctc ctgcccaatg atgtttaggg gaatggcctg
     1681 cgaaatccat caaaaaatat agccttcagg aaacaaagca aaccctggtt cacatggaag
     1741 ggggaaaaaa aaaggacaaa aaaaaaaaat cccaggactt tccaacttag catcttaccc
     1801 tggagctaat ccgcactgcc aagtggaaag cagcttgttt catggaaatc ctaccaacct
     1861 ctgatgctgt ctgttttaat ctacagtgcc tttctcccct ctttaatgcc tataatgctt
     1921 ccattttttc attccctaat gaagagtcag cagtgagata tgcccgggct gccttttccc
     1981 acaggcaatg ccaatctccc gctaataaaa caaaattaaa ttaaaaacag aatgttgtgc
     2041 taaaaggctt caagtcaccg ccaagctgca ttgtaggatt aatatattcc acccgtccca
     2101 tta
//
