LOCUS       NM_027062                804 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus complement component 8, gamma polypeptide (C8g),
            transcript variant 1, mRNA.
ACCESSION   NM_027062 XM_130127
VERSION     NM_027062.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 804)
  AUTHORS   Charkoftaki,G., Wang,Y., McAndrews,M., Bruford,E.A., Thompson,D.C.,
            Vasiliou,V. and Nebert,D.W.
  TITLE     Update on the human and mouse lipocalin (LCN) gene family,
            including evidence the mouse Mup cluster is result of an
            'evolutionary bloom'
  JOURNAL   Hum Genomics 13 (1), 11 (2019)
   PUBMED   30782214
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 804)
  AUTHORS   Menny,A., Serna,M., Boyd,C.M., Gardner,S., Joseph,A.P.,
            Morgan,B.P., Topf,M., Brooks,N.J. and Bubeck,D.
  TITLE     CryoEM reveals how the complement membrane attack complex ruptures
            lipid bilayers
  JOURNAL   Nat Commun 9 (1), 5316 (2018)
   PUBMED   30552328
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 804)
  AUTHORS   Slade,D.J., Lovelace,L.L., Chruszcz,M., Minor,W., Lebioda,L. and
            Sodetz,J.M.
  TITLE     Crystal structure of the MACPF domain of human complement protein
            C8 alpha in complex with the C8 gamma subunit
  JOURNAL   J Mol Biol 379 (2), 331-342 (2008)
   PUBMED   18440555
  REMARK    GeneRIF: Results describe the crystal structure of the human
            ortholog C8 alpha MACPF domain disulfide-linked to C8 gamma
            (alphaMACPF-gamma) at 2.15 A resolution.
REFERENCE   5  (bases 1 to 804)
  AUTHORS   Parker,C.L. and Sodetz,J.M.
  TITLE     Role of the human C8 subunits in complement-mediated bacterial
            killing: evidence that C8 gamma is not essential
  JOURNAL   Mol Immunol 39 (7-8), 453-458 (2002)
   PUBMED   12413696
  REMARK    GeneRIF: This is a study of the human orthologs of C8 alpha, beta,
            and gamma. C8 gamma is not required for complement-mediated killing
            of Gram-negative bacteria.
REFERENCE   6  (bases 1 to 804)
  AUTHORS   Musingarimi,P., Plumb,M.E. and Sodetz,J.M.
  TITLE     Interaction between the C8 alpha-gamma and C8 beta subunits of
            human complement C8: role of the C8 beta N-terminal thrombospondin
            type 1 module and membrane attack complex/perforin domain
  JOURNAL   Biochemistry 41 (37), 11255-11260 (2002)
   PUBMED   12220191
  REMARK    GeneRIF: This is a study of the human C8 orthologs. Binding of C8
            gamma subunit to C8 beta is dependent on the thrombospondin type 1
            module, low-density lipoprotein receptor class A module, and
            membrane attack complex/perforin domain of C8 beta.
REFERENCE   7  (bases 1 to 804)
  AUTHORS   Schreck,S.F., Parker,C., Plumb,M.E. and Sodetz,J.M.
  TITLE     Human complement protein C8 gamma
  JOURNAL   Biochim Biophys Acta 1482 (1-2), 199-208 (2000)
   PUBMED   11058761
  REMARK    GeneRIF: A review of the human C8 gamma ortholog.
            Review article
REFERENCE   8  (bases 1 to 804)
  AUTHORS   Chan,P., Simon-Chazottes,D., Mattei,M.G., Guenet,J.L. and
            Salier,J.P.
  TITLE     Comparative mapping of lipocalin genes in human and mouse: the four
            genes for complement C8 gamma chain, prostaglandin-D-synthase,
            oncogene-24p3, and progestagen-associated endometrial protein map
            to HSA9 and MMU2
  JOURNAL   Genomics 23 (1), 145-150 (1994)
   PUBMED   7829063
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL732557.4.
            
            On Jan 6, 2022 this sequence version replaced NM_027062.2.
            
            Summary: This gene encodes a secreted protein that is a core
            component of the complement 8 (C8) complex. C8 is part of the
            membrane attack complex which participates in the innate immune
            response against bacterial pathogens. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AI195106.1, CA459044.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-174               AL732557.4         172867-173040       c
            175-311             AL732557.4         172612-172748       c
            312-382             AL732557.4         172362-172432       c
            383-490             AL732557.4         172171-172278       c
            491-592             AL732557.4         171787-171888       c
            593-631             AL732557.4         171678-171716       c
            632-804             AL732557.4         171424-171596       c
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 17.31 cM"
     gene            1..804
                     /gene="C8g"
                     /note="complement component 8, gamma polypeptide"
                     /db_xref="GeneID:69379"
                     /db_xref="MGI:MGI:88237"
     exon            1..174
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..645
                     /gene="C8g"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; complement component C8 gamma chain; complement
                     component 8, gamma subunit; novel lipocalin protein"
                     /codon_start=1
                     /product="complement component C8 gamma chain isoform 1
                     precursor"
                     /protein_id="NP_081338.1"
                     /db_xref="CCDS:CCDS15777.1"
                     /db_xref="GeneID:69379"
                     /db_xref="MGI:MGI:88237"
                     /translation="MLSPGAVLFFTLLLTASSLGQRTRKPIGSTSPISTIQAQVNFSA
                     QKFAGTWLLVAVGSSCRFLQEQGHRAEATTLHAAPQGAAMAVSTFRKLDGICWQVRQL
                     FENTGVPGRFLFQVSRARGPVHMVVAETDYQSFAILYLEQGRKLSVKLYVRSLPVNDS
                     VLDVFERRVREANLTEDQILFFPKYGFCETADQLHILNEVPR"
     exon            175..311
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            312..382
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            383..490
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            491..592
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            593..631
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            632..804
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     regulatory      781..786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C8g"
                     /note="hexamer: ATTAAA"
     polyA_site      804
                     /gene="C8g"
                     /note="major polyA site"
ORIGIN      
        1 gctgtcctgt cacctactgt ccgtagcagg ttggccatgc tgtctccagg ggctgtgttg
       61 ttcttcaccc tgctcttgac agccagttcg ctgggccaaa ggactcgaaa gcctattggg
      121 tctacctccc ccatcagcac tatccaggcc caggtcaatt tcagtgctca gaagtttgca
      181 gggacatggc tcctggtggc tgtgggctct tcgtgccgct ttcttcagga gcagggacac
      241 cgagctgagg ccaccacatt gcacgcagct ccccagggtg cagctatggc tgtcagcacc
      301 ttccgaaagc tagatgggat atgttggcag gtacgccaac tctttgagaa cacaggggtc
      361 cctggccgct tcttgttcca agtctcacgg gcccgtggac cagtgcatat ggtggttgct
      421 gagactgact atcagagctt tgccatcctg tacctggaac agggaaggaa gctgtctgtg
      481 aaactatacg tccgctcact gccagtgaat gactctgtcc tggatgtgtt tgagcggcga
      541 gtcagggaag ccaacctgac agaagatcag attcttttct ttcccaagta tggtttctgc
      601 gagactgcgg accaattaca catcctgaac gaggtgccgc gataaggcca tctagtggag
      661 aagctgtgga cagtgggcta ccctcagaga gttctgagca tcctgaatgc actcttggag
      721 tctctccttt ctgtccccag aagcacccct tcatacagct ctgttgtcct aggtcttttc
      781 attaaacatc agacttggcc ctgg
//
