LOCUS       NM_001271777             823 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus complement component 8, gamma polypeptide (C8g),
            transcript variant 2, mRNA.
ACCESSION   NM_001271777
VERSION     NM_001271777.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 823)
  AUTHORS   Charkoftaki,G., Wang,Y., McAndrews,M., Bruford,E.A., Thompson,D.C.,
            Vasiliou,V. and Nebert,D.W.
  TITLE     Update on the human and mouse lipocalin (LCN) gene family,
            including evidence the mouse Mup cluster is result of an
            'evolutionary bloom'
  JOURNAL   Hum Genomics 13 (1), 11 (2019)
   PUBMED   30782214
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 823)
  AUTHORS   Menny,A., Serna,M., Boyd,C.M., Gardner,S., Joseph,A.P.,
            Morgan,B.P., Topf,M., Brooks,N.J. and Bubeck,D.
  TITLE     CryoEM reveals how the complement membrane attack complex ruptures
            lipid bilayers
  JOURNAL   Nat Commun 9 (1), 5316 (2018)
   PUBMED   30552328
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 823)
  AUTHORS   Slade,D.J., Lovelace,L.L., Chruszcz,M., Minor,W., Lebioda,L. and
            Sodetz,J.M.
  TITLE     Crystal structure of the MACPF domain of human complement protein
            C8 alpha in complex with the C8 gamma subunit
  JOURNAL   J Mol Biol 379 (2), 331-342 (2008)
   PUBMED   18440555
  REMARK    GeneRIF: Results describe the crystal structure of the human
            ortholog C8 alpha MACPF domain disulfide-linked to C8 gamma
            (alphaMACPF-gamma) at 2.15 A resolution.
REFERENCE   5  (bases 1 to 823)
  AUTHORS   Parker,C.L. and Sodetz,J.M.
  TITLE     Role of the human C8 subunits in complement-mediated bacterial
            killing: evidence that C8 gamma is not essential
  JOURNAL   Mol Immunol 39 (7-8), 453-458 (2002)
   PUBMED   12413696
  REMARK    GeneRIF: This is a study of the human orthologs of C8 alpha, beta,
            and gamma. C8 gamma is not required for complement-mediated killing
            of Gram-negative bacteria.
REFERENCE   6  (bases 1 to 823)
  AUTHORS   Musingarimi,P., Plumb,M.E. and Sodetz,J.M.
  TITLE     Interaction between the C8 alpha-gamma and C8 beta subunits of
            human complement C8: role of the C8 beta N-terminal thrombospondin
            type 1 module and membrane attack complex/perforin domain
  JOURNAL   Biochemistry 41 (37), 11255-11260 (2002)
   PUBMED   12220191
  REMARK    GeneRIF: This is a study of the human C8 orthologs. Binding of C8
            gamma subunit to C8 beta is dependent on the thrombospondin type 1
            module, low-density lipoprotein receptor class A module, and
            membrane attack complex/perforin domain of C8 beta.
REFERENCE   7  (bases 1 to 823)
  AUTHORS   Schreck,S.F., Parker,C., Plumb,M.E. and Sodetz,J.M.
  TITLE     Human complement protein C8 gamma
  JOURNAL   Biochim Biophys Acta 1482 (1-2), 199-208 (2000)
   PUBMED   11058761
  REMARK    GeneRIF: A review of the human C8 gamma ortholog.
            Review article
REFERENCE   8  (bases 1 to 823)
  AUTHORS   Chan,P., Simon-Chazottes,D., Mattei,M.G., Guenet,J.L. and
            Salier,J.P.
  TITLE     Comparative mapping of lipocalin genes in human and mouse: the four
            genes for complement C8 gamma chain, prostaglandin-D-synthase,
            oncogene-24p3, and progestagen-associated endometrial protein map
            to HSA9 and MMU2
  JOURNAL   Genomics 23 (1), 145-150 (1994)
   PUBMED   7829063
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK005962.1 and AL732557.4.
            
            Summary: This gene encodes a secreted protein that is a core
            component of the complement 8 (C8) complex. C8 is part of the
            membrane attack complex which participates in the innate immune
            response against bacterial pathogens. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at an
            alternate start codon, compared to variant 1. The encoded isoform
            (2) is shorter and has a distinct N-terminus, compared to isoform
            1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK005962.1, SRR13422600.1822353.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-822               AK005962.1         5-826
            823-823             AL732557.4         171423-171423       c
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 17.31 cM"
     gene            1..823
                     /gene="C8g"
                     /note="complement component 8, gamma polypeptide"
                     /db_xref="GeneID:69379"
                     /db_xref="MGI:MGI:88237"
     exon            1..192
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..663
                     /gene="C8g"
                     /note="isoform 2 is encoded by transcript variant 2;
                     complement component C8 gamma chain; complement component
                     8, gamma subunit; novel lipocalin protein"
                     /codon_start=1
                     /product="complement component C8 gamma chain isoform 2"
                     /protein_id="NP_001258706.1"
                     /db_xref="CCDS:CCDS71001.1"
                     /db_xref="GeneID:69379"
                     /db_xref="MGI:MGI:88237"
                     /translation="MPSPMLQGTGRQFAGTWLLVAVGSSCRFLQEQGHRAEATTLHAA
                     PQGAAMAVSTFRKLDGICWQVRQLFENTGVPGRFLFQVSRARGPVHMVVAETDYQSFA
                     ILYLEQGRKLSVKLYVRSLPVNDSVLDVFERRVREANLTEDQILFFPKYGFCETADQL
                     HILNEVPR"
     exon            193..329
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            330..400
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            401..508
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            509..610
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            611..649
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     exon            650..823
                     /gene="C8g"
                     /inference="alignment:Splign:2.1.0"
     regulatory      799..804
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C8g"
                     /note="hexamer: ATTAAA"
     polyA_site      822
                     /gene="C8g"
                     /note="major polyA site"
ORIGIN      
        1 acttcttgaa ggacccaggc agtaacgttg caccacctta cattctccag gacaccaaag
       61 gacttgctgg accagacagc aagcccctga gtactactag gccaggtaga acctgcgaag
      121 tggggggcag ccccactttc caggtggggg cctttgatgc cttccccaat gctacagggc
      181 acagggaggc agtttgcagg gacatggctc ctggtggctg tgggctcttc gtgccgcttt
      241 cttcaggagc agggacaccg agctgaggcc accacattgc acgcagctcc ccagggtgca
      301 gctatggctg tcagcacctt ccgaaagcta gatgggatat gttggcaggt acgccaactc
      361 tttgagaaca caggggtccc tggccgcttc ttgttccaag tctcacgggc ccgtggacca
      421 gtgcatatgg tggttgctga gactgactat cagagctttg ccatcctgta cctggaacag
      481 ggaaggaagc tgtctgtgaa actatacgtc cgctcactgc cagtgaatga ctctgtcctg
      541 gatgtgtttg agcggcgagt cagggaagcc aacctgacag aagatcagat tcttttcttt
      601 cccaagtatg gtttctgcga gactgcggac caattacaca tcctgaacga ggtgccgcga
      661 taaggccatc tagtggagaa gctgtggaca gtgggctacc ctcagagagt tctgagcatc
      721 ctgaatgcac tcttggagtc tctcctttct gtccccagaa gcaccccttc atacagctct
      781 gttgtcctag gtcttttcat taaacatcag acttggccct ggg
//
