LOCUS       NM_133882               2239 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus complement component 8, beta polypeptide (C8b),
            transcript variant 1, mRNA.
ACCESSION   NM_133882
VERSION     NM_133882.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Menny,A., Serna,M., Boyd,C.M., Gardner,S., Joseph,A.P.,
            Morgan,B.P., Topf,M., Brooks,N.J. and Bubeck,D.
  TITLE     CryoEM reveals how the complement membrane attack complex ruptures
            lipid bilayers
  JOURNAL   Nat Commun 9 (1), 5316 (2018)
   PUBMED   30552328
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2239)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 2239)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 2239)
  AUTHORS   Thoren,L.A., Norgaard,G.A., Weischenfeldt,J., Waage,J.,
            Jakobsen,J.S., Damgaard,I., Bergstrom,F.C., Blom,A.M., Borup,R.,
            Bisgaard,H.C. and Porse,B.T.
  TITLE     UPF2 is a critical regulator of liver development, function and
            regeneration
  JOURNAL   PLoS One 5 (7), e11650 (2010)
   PUBMED   20657840
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2239)
  AUTHORS   Bar,I., Tissir,F., Lambert de Rouvroit,C., De Backer,O. and
            Goffinet,A.M.
  TITLE     The gene encoding disabled-1 (DAB1), the intracellular adaptor of
            the Reelin pathway, reveals unusual complexity in human and mouse
  JOURNAL   J Biol Chem 278 (8), 5802-5812 (2003)
   PUBMED   12446734
REFERENCE   7  (bases 1 to 2239)
  AUTHORS   Truett,G.E., Jacob,H.J., Miller,J., Drouin,G., Bahary,N.,
            Smoller,J.W., Lander,E.S. and Leibel,R.L.
  TITLE     Genetic map of rat chromosome 5 including the fatty (fa) locus
  JOURNAL   Mamm Genome 6 (1), 25-30 (1995)
   PUBMED   7719022
REFERENCE   8  (bases 1 to 2239)
  AUTHORS   Bahary,N., McGraw,D.E., Shilling,R. and Friedman,J.M.
  TITLE     Microdissection and microcloning of mid-chromosome 4: genetic
            mapping of 41 microdissection clones
  JOURNAL   Genomics 16 (1), 113-122 (1993)
   PUBMED   8098006
REFERENCE   9  (bases 1 to 2239)
  AUTHORS   Bahary,N., Zorich,G., Pachter,J.E., Leibel,R.L. and Friedman,J.M.
  TITLE     Molecular genetic linkage maps of mouse chromosomes 4 and 6
  JOURNAL   Genomics 11 (1), 33-47 (1991)
   PUBMED   1684952
REFERENCE   10 (bases 1 to 2239)
  AUTHORS   Tanaka,S., Suzuki,T., Sakaizumi,M., Harada,Y., Matsushima,Y.,
            Miyashita,N., Fukumori,Y., Inai,S., Moriwaki,K. and Yonekawa,H.
  TITLE     Gene responsible for deficient activity of the beta subunit of C8,
            the eighth component of complement, is located on mouse chromosome
            4
  JOURNAL   Immunogenetics 33 (1), 18-23 (1991)
   PUBMED   1995474
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK050313.1.
            
            On Apr 6, 2007 this sequence version replaced NM_133882.1.
            
            Summary: This gene encodes the beta subunit of complement component
            C8 that participates in the assembly of the complement membrane
            attack complex. The encoded preproprotein undergoes proteolytic
            processing to generate the beta subunit, which associates with the
            alpha and gamma subunits to form a trimeric complement component,
            C8. Alternative splicing results in multiple transcript variants
            encoding different isoforms that may undergo similar proteolytic
            processing. This gene is located adjacent to the gene encoding the
            alpha subunit. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050313.1, BC096382.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2239              AK050313.1         2-2240
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 48.61 cM"
     gene            1..2239
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /note="complement component 8, beta polypeptide"
                     /db_xref="GeneID:110382"
                     /db_xref="MGI:MGI:88236"
     exon            1..170
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1851
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; complement component C8 beta chain; complement
                     component 8, beta subunit"
                     /codon_start=1
                     /product="complement component C8 beta chain isoform 1
                     preproprotein"
                     /protein_id="NP_598643.1"
                     /db_xref="CCDS:CCDS18414.1"
                     /db_xref="GeneID:110382"
                     /db_xref="MGI:MGI:88236"
                     /translation="MKIGAQVWRALAKSCLLCATLGCLHFPGSRGGKPDFFETKAVNG
                     SLVKSRPVRSVAEAPAPIDCELSTWSSWTACDPCQKKRYRHTYLLRPSQFYGELCDLS
                     DKEVEDCVTNQPCRSQVRCEGFVCAQTGRCVNRRLLCNGDNDCGDQSDEANCRRIYKN
                     CQREMEQYWAIDRLASGINLFTNTFEGPVLDHRYYAGGCSPHYILDTNFRKPYNVESY
                     TPQTKCEYEFTLTEYESYSDFERLVIEKKTHMFNFTSGFKVDGVMDLGIKVESNEGKN
                     YVTRTKRFAHTQSKFLHARSVLEVAHYKLKSRSLMLHYEFLQRVKSLPLEYSYGEYRD
                     LLRDFGTHFITEAVLGGIYEYTLIMNKDAMEQGDYTLSHVTACAGGSFGIGGMVYKVY
                     VKVGVSAKKCSDIMKEINERNKRSTMVEDLVVLVRGGTSEDITALAYKELPTPELMEA
                     WGDAVKYNPAIIKIKAEPLYELVTATDFAYSSTVKQNLKKALEEFQSEVSSCRCAPCR
                     GNGVPVLKGSRCECICPGGFQGTACEVTYRKDIPIDGKWSCWSDWSACSGGHKTRHRQ
                     CNNPAPHKGGSPCSGPASETLNC"
     exon            171..327
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            328..469
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            470..611
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            612..744
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            745..942
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1183
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1312
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1476
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1630
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1699
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..2239
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2004..2009
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      2032
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /note="major polyA site"
ORIGIN      
        1 aacagaactg agagcagcaa gagcgagcag gctatttcaa ccatcctcga atcacactgg
       61 atctcccgct acacagaaaa tatgaagata ggggctcagg tttggagggc actggcaaag
      121 tcgtgtctcc tctgtgctac tctgggatgt ctccatttcc ctggttccag aggtgggaag
      181 cccgatttct ttgagacaaa ggcagtcaat gggagccttg tcaagagcag accagtacgg
      241 agtgtggcgg aagccccagc acctattgac tgtgagctat ccacctggtc ttcctggact
      301 gcatgtgacc cctgtcagaa gaaaaggtac agacatactt acttgctccg gccctctcag
      361 ttctatgggg aattgtgtga cttgtccgac aaggaagttg aagactgtgt taccaaccaa
      421 ccctgcagga gtcaagtacg atgtgaaggc tttgtgtgtg cacagacagg gaggtgtgtg
      481 aatcgcagac tcctttgcaa tggggacaat gactgtggag accaatcaga tgaagccaac
      541 tgtaggagga tttataaaaa ctgtcaacgg gagatggagc agtactgggc aattgacagg
      601 ctggccagtg ggataaatct gttcacaaac acttttgagg gcccagttct cgatcaccgg
      661 tattatgctg gtggatgctc cccccactac atcctggaca ccaactttag gaagccatac
      721 aacgtagaaa gctacacccc acagaccaaa tgtgaatatg aattcacact tacagaatat
      781 gaatcatatt cagattttga acgtctagtg atagaaaaga aaacccacat gtttaatttc
      841 acgtctggtt ttaaagtgga tggcgttatg gatcttggaa ttaaagtaga aagcaacgaa
      901 ggcaaaaatt atgtgaccag aaccaaacgc ttcgcccaca ctcaaagcaa gttcctccac
      961 gcgcgctctg tccttgaagt ggcacattac aagctgaagt ctagaagcct catgctccat
     1021 tatgaattcc ttcagagggt caagagcctg cccctggagt atagctatgg ggaataccga
     1081 gatcttcttc gtgacttcgg gacccatttc atcactgaag ccgtacttgg gggcatctac
     1141 gagtacacgc ttatcatgaa caaagacgcc atggagcaag gagactacac tcttagccat
     1201 gttactgcct gtgctggagg aagtttcggt attggtggta tggtctataa agtctatgtc
     1261 aaagtgggcg tttccgcaaa gaaatgcagt gacattatga aagaaatcaa cgaaagaaat
     1321 aagaggagca ccatggtaga agacttggta gttcttgtga gaggagggac aagtgaggat
     1381 atcactgcct tggcatacaa ggagctacca acccctgaac tgatggaggc atggggcgat
     1441 gctgtgaagt acaacccagc aatcattaag atcaaggctg agcccctgta tgagcttgtg
     1501 acagccacag actttgcata ctccagcaca gtgaagcaga acttaaagaa ggcactagaa
     1561 gaattccaga gtgaggtcag ctcctgccgc tgtgctcctt gcagagggaa tggagtccct
     1621 gtcctgaaag gatcccgctg tgagtgcatc tgccccggtg gcttccaagg cacagcctgt
     1681 gaggttacct atcggaaaga catccccata gatgggaagt ggagttgctg gtctgactgg
     1741 tctgcatgct ctggaggaca caaaacaaga cacaggcagt gcaacaatcc agcccctcac
     1801 aaaggaggca gcccctgttc gggtcctgct tccgaaacac tcaactgtta aaggagggca
     1861 cacacagatg atcccgagtg aactccaacc ctcacacact tagccaggct tccagtactc
     1921 cagctcccac ccagggctgt cacaacaaaa cgcaatgcca ctctgccctt ttaagatttg
     1981 tttgatcagt gcatgataat ttgagtaaac agtggattga gcaaaaccct aagtccttgc
     2041 atggtgaacc tccctttgca cactctgcac ttttgttaca tccacaaggt cttggagcca
     2101 aaaactctct tggtcaccat gtagtgacaa agacacatag taggtgttga ataatgaaac
     2161 agtacattga taagttgttc atcccttggt aagcacagag tcttcttcag taataaagac
     2221 agccaatctc taggaaatg
//
