LOCUS       NM_001316671            2041 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus complement component 8, beta polypeptide (C8b),
            transcript variant 2, mRNA.
ACCESSION   NM_001316671
VERSION     NM_001316671.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Menny,A., Serna,M., Boyd,C.M., Gardner,S., Joseph,A.P.,
            Morgan,B.P., Topf,M., Brooks,N.J. and Bubeck,D.
  TITLE     CryoEM reveals how the complement membrane attack complex ruptures
            lipid bilayers
  JOURNAL   Nat Commun 9 (1), 5316 (2018)
   PUBMED   30552328
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2041)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 2041)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 2041)
  AUTHORS   Thoren,L.A., Norgaard,G.A., Weischenfeldt,J., Waage,J.,
            Jakobsen,J.S., Damgaard,I., Bergstrom,F.C., Blom,A.M., Borup,R.,
            Bisgaard,H.C. and Porse,B.T.
  TITLE     UPF2 is a critical regulator of liver development, function and
            regeneration
  JOURNAL   PLoS One 5 (7), e11650 (2010)
   PUBMED   20657840
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2041)
  AUTHORS   Bar,I., Tissir,F., Lambert de Rouvroit,C., De Backer,O. and
            Goffinet,A.M.
  TITLE     The gene encoding disabled-1 (DAB1), the intracellular adaptor of
            the Reelin pathway, reveals unusual complexity in human and mouse
  JOURNAL   J Biol Chem 278 (8), 5802-5812 (2003)
   PUBMED   12446734
REFERENCE   7  (bases 1 to 2041)
  AUTHORS   Truett,G.E., Jacob,H.J., Miller,J., Drouin,G., Bahary,N.,
            Smoller,J.W., Lander,E.S. and Leibel,R.L.
  TITLE     Genetic map of rat chromosome 5 including the fatty (fa) locus
  JOURNAL   Mamm Genome 6 (1), 25-30 (1995)
   PUBMED   7719022
REFERENCE   8  (bases 1 to 2041)
  AUTHORS   Bahary,N., McGraw,D.E., Shilling,R. and Friedman,J.M.
  TITLE     Microdissection and microcloning of mid-chromosome 4: genetic
            mapping of 41 microdissection clones
  JOURNAL   Genomics 16 (1), 113-122 (1993)
   PUBMED   8098006
REFERENCE   9  (bases 1 to 2041)
  AUTHORS   Bahary,N., Zorich,G., Pachter,J.E., Leibel,R.L. and Friedman,J.M.
  TITLE     Molecular genetic linkage maps of mouse chromosomes 4 and 6
  JOURNAL   Genomics 11 (1), 33-47 (1991)
   PUBMED   1684952
REFERENCE   10 (bases 1 to 2041)
  AUTHORS   Tanaka,S., Suzuki,T., Sakaizumi,M., Harada,Y., Matsushima,Y.,
            Miyashita,N., Fukumori,Y., Inai,S., Moriwaki,K. and Yonekawa,H.
  TITLE     Gene responsible for deficient activity of the beta subunit of C8,
            the eighth component of complement, is located on mouse chromosome
            4
  JOURNAL   Immunogenetics 33 (1), 18-23 (1991)
   PUBMED   1995474
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK050313.1.
            
            Summary: This gene encodes the beta subunit of complement component
            C8 that participates in the assembly of the complement membrane
            attack complex. The encoded preproprotein undergoes proteolytic
            processing to generate the beta subunit, which associates with the
            alpha and gamma subunits to form a trimeric complement component,
            C8. Alternative splicing results in multiple transcript variants
            encoding different isoforms that may undergo similar proteolytic
            processing. This gene is located adjacent to the gene encoding the
            alpha subunit. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            internal exon, compared to variant 1. It encodes isoform 2, which
            is shorter than isoform 1. This isoform (2) may under proteolytic
            processing similar to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB077306.1, ERR2844020.538165.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-744               AK050313.1         2-745
            745-2041            AK050313.1         944-2240
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 48.61 cM"
     gene            1..2041
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /note="complement component 8, beta polypeptide"
                     /db_xref="GeneID:110382"
                     /db_xref="MGI:MGI:88236"
     exon            1..170
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1653
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; complement component C8 beta chain; complement
                     component 8, beta subunit"
                     /codon_start=1
                     /product="complement component C8 beta chain isoform 2
                     precursor"
                     /protein_id="NP_001303600.1"
                     /db_xref="CCDS:CCDS84762.1"
                     /db_xref="GeneID:110382"
                     /db_xref="MGI:MGI:88236"
                     /translation="MKIGAQVWRALAKSCLLCATLGCLHFPGSRGGKPDFFETKAVNG
                     SLVKSRPVRSVAEAPAPIDCELSTWSSWTACDPCQKKRYRHTYLLRPSQFYGELCDLS
                     DKEVEDCVTNQPCRSQVRCEGFVCAQTGRCVNRRLLCNGDNDCGDQSDEANCRRIYKN
                     CQREMEQYWAIDRLASGINLFTNTFEGPVLDHRYYAGGCSPHYILDTNFRKPYNVESY
                     TPQQSKFLHARSVLEVAHYKLKSRSLMLHYEFLQRVKSLPLEYSYGEYRDLLRDFGTH
                     FITEAVLGGIYEYTLIMNKDAMEQGDYTLSHVTACAGGSFGIGGMVYKVYVKVGVSAK
                     KCSDIMKEINERNKRSTMVEDLVVLVRGGTSEDITALAYKELPTPELMEAWGDAVKYN
                     PAIIKIKAEPLYELVTATDFAYSSTVKQNLKKALEEFQSEVSSCRCAPCRGNGVPVLK
                     GSRCECICPGGFQGTACEVTYRKDIPIDGKWSCWSDWSACSGGHKTRHRQCNNPAPHK
                     GGSPCSGPASETLNC"
     exon            171..327
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            328..469
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            470..611
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            612..744
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            745..985
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1114
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1278
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1432
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1501
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..2041
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1806..1811
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      1834
                     /gene="C8b"
                     /gene_synonym="4930439B20Rik"
                     /note="major polyA site"
ORIGIN      
        1 aacagaactg agagcagcaa gagcgagcag gctatttcaa ccatcctcga atcacactgg
       61 atctcccgct acacagaaaa tatgaagata ggggctcagg tttggagggc actggcaaag
      121 tcgtgtctcc tctgtgctac tctgggatgt ctccatttcc ctggttccag aggtgggaag
      181 cccgatttct ttgagacaaa ggcagtcaat gggagccttg tcaagagcag accagtacgg
      241 agtgtggcgg aagccccagc acctattgac tgtgagctat ccacctggtc ttcctggact
      301 gcatgtgacc cctgtcagaa gaaaaggtac agacatactt acttgctccg gccctctcag
      361 ttctatgggg aattgtgtga cttgtccgac aaggaagttg aagactgtgt taccaaccaa
      421 ccctgcagga gtcaagtacg atgtgaaggc tttgtgtgtg cacagacagg gaggtgtgtg
      481 aatcgcagac tcctttgcaa tggggacaat gactgtggag accaatcaga tgaagccaac
      541 tgtaggagga tttataaaaa ctgtcaacgg gagatggagc agtactgggc aattgacagg
      601 ctggccagtg ggataaatct gttcacaaac acttttgagg gcccagttct cgatcaccgg
      661 tattatgctg gtggatgctc cccccactac atcctggaca ccaactttag gaagccatac
      721 aacgtagaaa gctacacccc acagcaaagc aagttcctcc acgcgcgctc tgtccttgaa
      781 gtggcacatt acaagctgaa gtctagaagc ctcatgctcc attatgaatt ccttcagagg
      841 gtcaagagcc tgcccctgga gtatagctat ggggaatacc gagatcttct tcgtgacttc
      901 gggacccatt tcatcactga agccgtactt gggggcatct acgagtacac gcttatcatg
      961 aacaaagacg ccatggagca aggagactac actcttagcc atgttactgc ctgtgctgga
     1021 ggaagtttcg gtattggtgg tatggtctat aaagtctatg tcaaagtggg cgtttccgca
     1081 aagaaatgca gtgacattat gaaagaaatc aacgaaagaa ataagaggag caccatggta
     1141 gaagacttgg tagttcttgt gagaggaggg acaagtgagg atatcactgc cttggcatac
     1201 aaggagctac caacccctga actgatggag gcatggggcg atgctgtgaa gtacaaccca
     1261 gcaatcatta agatcaaggc tgagcccctg tatgagcttg tgacagccac agactttgca
     1321 tactccagca cagtgaagca gaacttaaag aaggcactag aagaattcca gagtgaggtc
     1381 agctcctgcc gctgtgctcc ttgcagaggg aatggagtcc ctgtcctgaa aggatcccgc
     1441 tgtgagtgca tctgccccgg tggcttccaa ggcacagcct gtgaggttac ctatcggaaa
     1501 gacatcccca tagatgggaa gtggagttgc tggtctgact ggtctgcatg ctctggagga
     1561 cacaaaacaa gacacaggca gtgcaacaat ccagcccctc acaaaggagg cagcccctgt
     1621 tcgggtcctg cttccgaaac actcaactgt taaaggaggg cacacacaga tgatcccgag
     1681 tgaactccaa ccctcacaca cttagccagg cttccagtac tccagctccc acccagggct
     1741 gtcacaacaa aacgcaatgc cactctgccc ttttaagatt tgtttgatca gtgcatgata
     1801 atttgagtaa acagtggatt gagcaaaacc ctaagtcctt gcatggtgaa cctccctttg
     1861 cacactctgc acttttgtta catccacaag gtcttggagc caaaaactct cttggtcacc
     1921 atgtagtgac aaagacacat agtaggtgtt gaataatgaa acagtacatt gataagttgt
     1981 tcatcccttg gtaagcacag agtcttcttc agtaataaag acagccaatc tctaggaaat
     2041 g
//
