LOCUS       NP_666260                587 aa            linear   ROD 14-FEB-2025
DEFINITION  complement component C8 alpha chain isoform 1 preproprotein [Mus
            musculus].
ACCESSION   NP_666260
VERSION     NP_666260.1
DBSOURCE    REFSEQ: accession NM_146148.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 587)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 587)
  AUTHORS   Mohammed,Y., Michaud,S.A., Petrosova,H., Yang,J., Ganguly,M.,
            Schibli,D., Flenniken,A.M., Nutter,L.M.J., Adissu,H.A.,
            Lloyd,K.C.K., McKerlie,C. and Borchers,C.H.
  TITLE     Proteotyping of knockout mouse strains reveals sex- and
            strain-specific signatures in blood plasma
  JOURNAL   NPJ Syst Biol Appl 7 (1), 25 (2021)
   PUBMED   34050187
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 587)
  AUTHORS   Menny,A., Serna,M., Boyd,C.M., Gardner,S., Joseph,A.P.,
            Morgan,B.P., Topf,M., Brooks,N.J. and Bubeck,D.
  TITLE     CryoEM reveals how the complement membrane attack complex ruptures
            lipid bilayers
  JOURNAL   Nat Commun 9 (1), 5316 (2018)
   PUBMED   30552328
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 587)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (residues 1 to 587)
  AUTHORS   Bar,I., Tissir,F., Lambert de Rouvroit,C., De Backer,O. and
            Goffinet,A.M.
  TITLE     The gene encoding disabled-1 (DAB1), the intracellular adaptor of
            the Reelin pathway, reveals unusual complexity in human and mouse
  JOURNAL   J Biol Chem 278 (8), 5802-5812 (2003)
   PUBMED   12446734
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL627186.13.
            
            Summary: This gene encodes the alpha subunit of complement
            component C8 that participates in the assembly of the complement
            membrane attack complex. The encoded preproprotein undergoes
            proteolytic processing to generate the alpha subunit, which
            associates with the beta and gamma subunits to form a trimeric
            complement component, C8. Alternative splicing results in multiple
            transcript variants encoding different isoforms that may undergo
            similar proteolytic processing. This gene is located adjacent to
            the gene encoding the beta subunit. [provided by RefSeq, Oct 2015].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK090245.1, BC027748.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 48.69 cM"
     Protein         1..587
                     /product="complement component C8 alpha chain isoform 1
                     preproprotein"
                     /note="complement component C8 alpha chain; complement
                     component 8 alpha subunit"
                     /calculated_mol_wt=63949
     sig_peptide     1..20
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2150
     mat_peptide     31..587
                     /product="Complement component C8 alpha chain.
                     /id=PRO_0000023588"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K182.1)"
                     /calculated_mol_wt=62710
     Site            44
                     /site_type="glycosylation"
                     /note="C-linked (Man) tryptophan.
                     /evidence=ECO:0000250|UniProtKB:P07357; propagated from
                     UniProtKB/Swiss-Prot (Q8K182.1)"
     Region          95..130
                     /region_name="Ldl_recept_a"
                     /note="Low-density lipoprotein receptor domain class A;
                     pfam00057"
                     /db_xref="CDD:395011"
     Site            order(100,109,120..121)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(113,116,120,126..127)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            123..127
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          289..492
                     /region_name="MACPF"
                     /note="membrane-attack complex / perforin; smart00457"
                     /db_xref="CDD:214671"
     Site            437
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8K182.1)"
     Region          545..587
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Site            545
                     /site_type="glycosylation"
                     /note="C-linked (Man) tryptophan.
                     /evidence=ECO:0000250|UniProtKB:P07357; propagated from
                     UniProtKB/Swiss-Prot (Q8K182.1)"
     Site            548
                     /site_type="glycosylation"
                     /note="C-linked (Man) tryptophan.
                     /evidence=ECO:0000250|UniProtKB:P07357; propagated from
                     UniProtKB/Swiss-Prot (Q8K182.1)"
     Site            551
                     /site_type="glycosylation"
                     /note="C-linked (Man) tryptophan.
                     /evidence=ECO:0000250|UniProtKB:P07357; propagated from
                     UniProtKB/Swiss-Prot (Q8K182.1)"
     CDS             1..587
                     /gene="C8a"
                     /coded_by="NM_146148.3:40..1803"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS18415.1"
                     /db_xref="GeneID:230558"
                     /db_xref="MGI:MGI:2668347"
ORIGIN      
        1 mfvvaffgls lvawhpgvta qekvnqrvtr avtpqavscq lsdwykwtdc fpcqdkkyry
       61 rsllqpskfg gticsgdiwd eascdsptpc lrqaqcgqdf qcretgrclk rhlvcngdnd
      121 cldgsdesdc edvrvteddc hqyepipgse raalgynilt qeeaqsvyda kyyggqcetv
      181 yngdwrklry dptcerlyyg edekyfrkpy nflkyhfeal adtsissefy ddandlffhi
      241 kngkshsagv tvgvapvksp vsievtgsgs kassflnkln kynekrygfm rvstkiqtaq
      301 fkmrrnnivl degmlqslme lpeqfnygmy akfindygth yitsgtmggi yeyvmvldke
      361 kmktegttvd evqkcigggi gigikdstie gvgisgefce nsgdgdrdir kkitgvedii
      421 srvqggssvw gsvlthnssa ityqswgrsl kynpvvidfe mqpiyqllrh tnlgpletkr
      481 qnlrraldqy lmefnacrcg pcfnngepil dgtncrcqcs mgrqglacer tvieglkdfk
      541 aaghwscwss wsecrggsqe rrrqcnnppp knggtpclgr nlqtqac
//
