LOCUS       NM_176912               3520 bp    mRNA    linear   ROD 23-JUL-2024
DEFINITION  Mus musculus complement component 5a receptor 2 (C5ar2), transcript
            variant 1, mRNA.
ACCESSION   NM_176912
VERSION     NM_176912.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Zhang,T., Ma,N., Wang,J., Min,X., Wei,L. and Li,K.
  TITLE     C5aR2 Deficiency Lessens C5aR1 Distribution and Expression in
            Neutrophils and Macrophages
  JOURNAL   J Immunol Res 2024, 2899154 (2024)
   PUBMED   39021433
  REMARK    GeneRIF: C5aR2 Deficiency Lessens C5aR1 Distribution and Expression
            in Neutrophils and Macrophages.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3520)
  AUTHORS   Seiler,D.L., Kahler,K.H., Kleingarn,M., Sadik,C.D., Bieber,K.,
            Kohl,J., Ludwig,R.J. and Karsten,C.M.
  TITLE     The complement receptor C5aR2 regulates neutrophil activation and
            function contributing to neutrophil-driven epidermolysis bullosa
            acquisita
  JOURNAL   Front Immunol 14, 1197709 (2023)
   PUBMED   37275893
  REMARK    GeneRIF: The complement receptor C5aR2 regulates neutrophil
            activation and function contributing to neutrophil-driven
            epidermolysis bullosa acquisita.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3520)
  AUTHORS   Li,Y., Sha,Y., Wang,H., He,L., Li,L., Wen,S., Sheng,L., Hu,W. and
            Zhou,H.
  TITLE     Intracellular C3 prevents hepatic steatosis by promoting autophagy
            and very-low-density lipoprotein secretion
  JOURNAL   FASEB J 35 (12), e22037 (2021)
   PUBMED   34762761
REFERENCE   5  (bases 1 to 3520)
  AUTHORS   Nording,H., Baron,L., Haberthur,D., Emschermann,F., Mezger,M.,
            Sauter,M., Sauter,R., Patzelt,J., Knoepp,K., Nording,A., Meusel,M.,
            Meyer-Saraei,R., Hlushchuk,R., Sedding,D., Borst,O., Eitel,I.,
            Karsten,C.M., Feil,R., Pichler,B., Erdmann,J., Verschoor,A.,
            Chavakis,E., Chavakis,T., von Hundelshausen,P., Kohl,J., Gawaz,M.
            and Langer,H.F.
  TITLE     The C5a/C5a receptor 1 axis controls tissue neovascularization
            through CXCL4 release from platelets
  JOURNAL   Nat Commun 12 (1), 3352 (2021)
   PUBMED   34099640
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3520)
  AUTHORS   Scola,A.M., Higginbottom,A., Partridge,L.J., Reid,R.C.,
            Woodruff,T., Taylor,S.M., Fairlie,D.P. and Monk,P.N.
  TITLE     The role of the N-terminal domain of the complement fragment
            receptor C5L2 in ligand binding
  JOURNAL   J Biol Chem 282 (6), 3664-3671 (2007)
   PUBMED   17158873
  REMARK    GeneRIF: C5L2 appears to bind complement factor C5a and C5a des Arg
            by different mechanisms, and, unlike C5a receptor (C5aR), C5L2 uses
            critical residues in its N-terminal domain for binding only to C5a
            des Arg.
REFERENCE   7  (bases 1 to 3520)
  AUTHORS   Gerard,N.P., Lu,B., Liu,P., Craig,S., Fujiwara,Y., Okinaga,S. and
            Gerard,C.
  TITLE     An anti-inflammatory function for the complement anaphylatoxin
            C5a-binding protein, C5L2
  JOURNAL   J Biol Chem 280 (48), 39677-39680 (2005)
   PUBMED   16204243
  REMARK    GeneRIF: the complement anaphylatoxin C5a-binding protein, C5L2
            (GPR77) has an anti-inflammatory role
REFERENCE   8  (bases 1 to 3520)
  AUTHORS   Gao,H., Neff,T.A., Guo,R.F., Speyer,C.L., Sarma,J.V., Tomlins,S.,
            Man,Y., Riedemann,N.C., Hoesel,L.M., Younkin,E., Zetoune,F.S. and
            Ward,P.A.
  TITLE     Evidence for a functional role of the second C5a receptor C5L2
  JOURNAL   FASEB J 19 (8), 1003-1005 (2005)
   PUBMED   15784721
  REMARK    GeneRIF: data provide the first evidence for a role for C5a
            receptor C5L2 in balancing the biological responses to Complement
            5a
REFERENCE   9  (bases 1 to 3520)
  AUTHORS   Okinaga,S., Slattery,D., Humbles,A., Zsengeller,Z., Morteau,O.,
            Kinrade,M.B., Brodbeck,R.M., Krause,J.E., Choe,H.R., Gerard,N.P.
            and Gerard,C.
  TITLE     C5L2, a nonsignaling C5A binding protein
  JOURNAL   Biochemistry 42 (31), 9406-9415 (2003)
   PUBMED   12899627
REFERENCE   10 (bases 1 to 3520)
  AUTHORS   Valenzuela,D.M., Murphy,A.J., Frendewey,D., Gale,N.W.,
            Economides,A.N., Auerbach,W., Poueymirou,W.T., Adams,N.C.,
            Rojas,J., Yasenchak,J., Chernomorsky,R., Boucher,M., Elsasser,A.L.,
            Esau,L., Zheng,J., Griffiths,J.A., Wang,X., Su,H., Xue,Y.,
            Dominguez,M.G., Noguera,I., Torres,R., Macdonald,L.E.,
            Stewart,A.F., DeChiara,T.M. and Yancopoulos,G.D.
  TITLE     High-throughput engineering of the mouse genome coupled with
            high-resolution expression analysis
  JOURNAL   Nat Biotechnol 21 (6), 652-659 (2003)
   PUBMED   12730667
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156630.6.
            
            [WARNING] On Feb 3, 2025 this sequence was replaced by NM_176912.6.
            
            On May 2, 2019 this sequence version replaced NM_176912.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK053187.1, BC137941.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849379, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-132               AC156630.6         124709-124840       c
            133-3520            AC156630.6         115273-118660       c
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 8.77 cM"
     gene            1..3520
                     /gene="C5ar2"
                     /gene_synonym="C5L2; E030029A11Rik; Gpr77"
                     /note="complement component 5a receptor 2"
                     /db_xref="GeneID:319430"
                     /db_xref="MGI:MGI:2442013"
     exon            1..132
                     /gene="C5ar2"
                     /gene_synonym="C5L2; E030029A11Rik; Gpr77"
                     /inference="alignment:Splign:2.1.0"
     exon            133..3520
                     /gene="C5ar2"
                     /gene_synonym="C5L2; E030029A11Rik; Gpr77"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..1183
                     /gene="C5ar2"
                     /gene_synonym="C5L2; E030029A11Rik; Gpr77"
                     /note="isoform 1 is encoded by transcript variant 1; C5a
                     anaphylatoxin chemotactic receptor C5L2; G protein-coupled
                     receptor 77; C5a anaphylatoxin chemotactic receptor 2"
                     /codon_start=1
                     /product="C5a anaphylatoxin chemotactic receptor 2 isoform
                     1"
                     /protein_id="NP_795886.3"
                     /db_xref="CCDS:CCDS52036.2"
                     /db_xref="GeneID:319430"
                     /db_xref="MGI:MGI:2442013"
                     /translation="MMNHTTSEYYDYEYDHEHYSDLPDVPVDCPAGTCFTSDVYLIVL
                     LVLYAAVFLVGVPGNTLVAWVTWKESRHRLGASWFLHLTMADLLCCVSLPFLAVPIAQ
                     KGHWPYGAAGCWLLSSITILSMYASVLLLTGLSGDLFLLAFRPSWKGADHRTFGVRVV
                     QASSWMLGLLLTVPSAVYRRLLQEHYPPRLVCGIDYGGSVSAEVAITTVRFLFGFLGP
                     LVFMAGCHGILQRQMARRHWPLGTAVVVGFFICWTPYHVLRVIIAAAPPHSLLLARVL
                     EAEPLFNGLALAHSALNPIMFLYFGRKQLCKSLQAACHWALRDPQDEESAVTKVSIST
                     SHEMVSEMPV"
ORIGIN      
        1 agatgcaggg ctccaaggcc tggggccaga gccgccttcc cttgtaacgg tgacctccgg
       61 gagctcacat ccaggaagct gttacattgc agtagagtct tctgggatga aatatgaggg
      121 gctgggagac gggtgtctgg gggccagaat gatgaaccac accaccagcg agtattatga
      181 ctatgagtat gaccatgaac actacagtga ccttccggac gtgcccgtag actgcccagc
      241 tggcacctgc ttcactagtg atgtctacct gatagtcctg cttgtgctgt atgcagccgt
      301 cttcctggtg ggtgtgccag gcaacaccct ggtggcgtgg gtgacctgga aagagtcccg
      361 ccacaggctt ggggcctctt ggttcctgca cctgaccatg gccgacttgc tttgttgtgt
      421 gtctctgccc ttcctggctg tgcccatcgc ccaaaagggc cactggccat acggggcggc
      481 aggctgctgg ctgttgtcct ccatcaccat tctgtctatg tatgccagtg tgctgctcct
      541 gaccggcctc agcggagacc tcttcctact ggctttcagg ccctcctgga agggtgcaga
      601 tcatcgaaca tttggggtgc gtgtggtcca ggcctcctcc tggatgctgg gcctcttgct
      661 gactgtgcct tctgctgtct accgtaggct gcttcaggag cactatcctc cccgacttgt
      721 gtgtggcata gactatgggg gatcggtcag cgccgaggtt gccatcacca ctgttcgatt
      781 cctctttggc ttcctggggc cattggtgtt catggccggt tgtcacggca tcctccaacg
      841 gcagatggcc cgccgccatt ggcccctggg cacagctgtc gtggtggggt ttttcatttg
      901 ctggacccct tatcacgtcc tgcgggtaat cattgcagcg gctcctccgc actccttgct
      961 cctcgcccgg gtcctggagg cggagcctct gtttaatggc ctggccctgg ctcacagtgc
     1021 tctcaatccc ataatgtttt tgtatttcgg aagaaaacaa ctctgcaagt cactccaggc
     1081 tgcatgccac tgggccctga gggatccaca ggatgaggaa agtgcggtga ccaaggtatc
     1141 catatccacc agccacgaaa tggtgtctga gatgccggtg taggcaagag ggctttggat
     1201 ttcctaggcc atcgcacaac tgtgcagctt caagcatagc tggatgcagg agctcggaga
     1261 tggcttttgt tcttcattca atattcattg tgcacttgct cgttcaaaac cctgatataa
     1321 aggtcccggg ctgacagtgg ctggagccaa ttccttcccc aggagttagc agtttacaca
     1381 agaagacgta taggaaacac ttgacgggat atggactatg tcaaggttga gatgttccat
     1441 agaacccaaa gagggtgctg gggagactgc tcgagtgcct gctgtacaaa ccaggacctg
     1501 agtttgagcc ccaccacctt cacagagctg tgtatggtga ttcacctcta accccagcac
     1561 tgagggaata ggtggatcca aggggttctc tggacaagtg gtctagccca atcaacaaac
     1621 ccctgtctca agaaatagag tggagccagg cacggtggtg ataactttaa ttacagcatt
     1681 tgggaggtag agccaggcta gcctgatcta catagtgaga tcaggctagc ctgatctacg
     1741 tagtgaggtc cagatggaga tttgggaggt cgagccaggc tagcctgatc tacatagtga
     1801 gatccaggtg gagagtagca gatgaagaca tccagtctta accttaggga cacacacaca
     1861 cacacacaca cacacacaca cacacacaca ctacacaggg ttggacagcc taggtcaggt
     1921 aggctagcac cctgaacaac gggtaatgac tgagccaaaa cctgactaat ggacctgagc
     1981 atccatgctt aaatctgggg aactattcca agcagacaga gcaaacacaa aggccttgct
     2041 ctggggtatc tcttccaaga agccttccct gacttgcatc cctcccagaa acaagggttc
     2101 ttcctatctt gcaatctctt caacacaaat gcccagataa atcctaagtc ccctaagagt
     2161 gagagaggaa ctcactctat cttttatatt ttggtattac atttaattat ttattgcctg
     2221 tgtcggaaag catggaggga gtaggggtgg ggtgggacga tggttagagg acaactcttt
     2281 ccatcatatg ggtccttggg atcgaactca gggtgtcagg cttggttgta agcaccttta
     2341 ctggcggagc catctcacca gccccaaact cctccgtctt gtctctttct cccctcctct
     2401 gtcttagaaa gtactgcatt tcctctgtcc acactctgat tgttttcaac tgctcagtgg
     2461 gcctgtttgt gttgctggtg tcttttttgt tattttcgag gatctttaaa ctattttatt
     2521 ttattttttt ttaaaaatca tacaggtata tgctgtacat tgagtagatc cccaccttac
     2581 tccaggtttg cagaacttta acgcctatgg catactgtgt ccagccaact ctcccccaca
     2641 gatccctagg gcctggcttt ggtcttggtt ttcttttttg attttttttt tttttttttt
     2701 ttttgagata gggtctcata gagcccagga tggttttgaa ctcactatga agggggtgac
     2761 cttcatcatc ccctgaatgc tgggattata gtctgtatca ccacacgtta gtttatgggg
     2821 tgctggtgat caacctcagg gcttcatgca tgctaggcac atgctctatc aattgagcga
     2881 catctggatt tcctcacagg gggactctag gaagctgtcc tgttactgag ctccacccca
     2941 aaccctcctt gaacaatgta tacatctgtc tgtctgaaaa gttcaaaaaa gttttggtta
     3001 atttactgga ccagagagcc tcttcagaag gtcttttaaa ttttttcttt ctttcttttt
     3061 tttttttttt tggttttttg agacagggtt tctctgtgta gccctggctg tcctggaact
     3121 cactttgtag accaggctgg cctcgaactc agaaatccac ctgcctctgc ctcccgagcg
     3181 ttgggattaa aggcgtgcgc caccattgcc tggcaaaaat ttctttttga aagtacactt
     3241 tggtgttgta tgaaaggaga ggttagcact cccccttgac aaggatggaa gaggccttgg
     3301 gcctaacaac acacatacgg ttaaggcatt gcctattacc tatttcgtgg catctaacaa
     3361 aataaaagaa aaaacaaaaa caacaaaaaa aaaaagaaga aaaggaaagt acactttgag
     3421 agtttctatt taattcttta gtcttaaaac aatgaaggag aaatttcatg agatcttcca
     3481 aatgtatttt ctgtgtaact atacataaaa tctcttgatg
//
