LOCUS       NP_033910               1738 aa            linear   ROD 28-APR-2025
DEFINITION  complement C4-B precursor [Mus musculus].
ACCESSION   NP_033910 XP_915519 XP_926736 XP_926741 XP_926748 XP_926753
            XP_926756 XP_926766 XP_926769 XP_926771
VERSION     NP_033910.2
DBSOURCE    REFSEQ: accession NM_009780.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1738)
  AUTHORS   Stark,K., Kilani,B., Stockhausen,S., Busse,J., Schubert,I.,
            Tran,T.D., Gaertner,F., Leunig,A., Pekayvaz,K., Nicolai,L.,
            Fumagalli,V., Stermann,J., Stephan,F., David,C., Muller,M.B.,
            Heyman,B., Lux,A., da Palma Guerreiro,A., Frenzel,L.P.,
            Schmidt,C.Q., Dopler,A., Moser,M., Chandraratne,S., von Bruhl,M.L.,
            Lorenz,M., Korff,T., Rudelius,M., Popp,O., Kirchner,M., Mertins,P.,
            Nimmerjahn,F., Iannacone,M., Sperandio,M., Engelmann,B.,
            Verschoor,A. and Massberg,S.
  TITLE     Antibodies and complement are key drivers of thrombosis
  JOURNAL   Immunity 57 (9), 2140-2156 (2024)
   PUBMED   39226900
REFERENCE   2  (residues 1 to 1738)
  AUTHORS   Fournier,L.A., Phadke,R.A., Salgado,M., Brack,A., Nocon,J.C.,
            Bolshakova,S., Grant,J.R., Padro Luna,N.M., Sen,K. and
            Cruz-Martin,A.
  TITLE     Overexpression of the schizophrenia risk gene C4 in PV cells drives
            sex-dependent behavioral deficits and circuit dysfunction
  JOURNAL   iScience 27 (9), 110800 (2024)
   PUBMED   39310747
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 1738)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (residues 1 to 1738)
  AUTHORS   Veremeyko,T., Jiang,R., He,M. and Ponomarev,E.D.
  TITLE     Complement C4-deficient mice have a high mortality rate during
            PTZ-induced epileptic seizures, which correlates with cognitive
            problems and the deficiency in the expression of Egr1 and other
            immediate early genes
  JOURNAL   Front Cell Neurosci 17, 1170031 (2023)
   PUBMED   37234916
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 1738)
  AUTHORS   Zhou,T., Li,Y., Li,X., Zeng,F., Rao,Y., He,Y., Wang,Y., Liu,M.,
            Li,D., Xu,Z., Zhou,X., Du,S., Niu,F., Peng,J., Mei,X., Ji,S.J.,
            Shu,Y., Lu,W., Guo,F., Wu,T., Yuan,T.F., Mao,Y. and Peng,B.
  TITLE     Microglial debris is cleared by astrocytes via C4b-facilitated
            phagocytosis and degraded via RUBICON-dependent noncanonical
            autophagy in mice
  JOURNAL   Nat Commun 13 (1), 6233 (2022)
   PUBMED   36280666
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 1738)
  AUTHORS   Miller,R.D., Aizawa,Y. and Shreffler,D.C.
  TITLE     Restriction fragment length polymorphism of the murine C4 and Slp
            genes: two C4 groups
  JOURNAL   J Immunol 149 (9), 2926-2934 (1992)
   PUBMED   1357031
REFERENCE   7  (residues 1 to 1738)
  AUTHORS   Pattanakitsakul,S., Zheng,J.H., Natsuume-Sakai,S., Takahashi,M. and
            Nonaka,M.
  TITLE     Aberrant splicing caused by the insertion of the B2 sequence into
            an intron of the complement C4 gene is the basis for low C4
            production in H-2k mice
  JOURNAL   J Biol Chem 267 (11), 7814-7820 (1992)
   PUBMED   1373139
REFERENCE   8  (residues 1 to 1738)
  AUTHORS   Natsuume-Sakai,S., Hayakawa,J.I. and Takahashi,M.
  TITLE     Genetic polymorphism of murine C3 controlled by a single
            co-dominant locus on chromosome 17
  JOURNAL   J Immunol 121 (2), 491-498 (1978)
   PUBMED   681746
REFERENCE   9  (residues 1 to 1738)
  AUTHORS   Carroll,M.C. and Capra,J.D.
  TITLE     Studies on the murine Ss protein: demonstration that the Ss protein
            is functionally the fourth component of complement
  JOURNAL   Proc Natl Acad Sci U S A 75 (5), 2424-2428 (1978)
   PUBMED   276882
REFERENCE   10 (residues 1 to 1738)
  AUTHORS   Meo,T., Douglas,T. and Rijnbeek,A.M.
  TITLE     Glyoxalase I polymorphism in the mouse: a new genetic marker linked
            to H-2
  JOURNAL   Science 198 (4314), 311-313 (1977)
   PUBMED   910130
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY785731.1, BC067394.1, CA459454.1, AK158475.1, AK158256.1,
            AK128974.1, K02798.1 and AK157954.1.
            
            On Oct 6, 2007 this sequence version replaced NP_033910.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC067394.1, AK157954.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 18.29 cM"
     Protein         1..1738
                     /product="complement C4-B precursor"
                     /note="complement C4-B; complement component 4 (within
                     H-2S); complement component 4B (Childo blood group);
                     complement component 4B (Chido blood group)"
                     /calculated_mol_wt=190719
     sig_peptide     1..19
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2216
     mat_peptide     20..673
                     /product="Complement C4 beta chain. /id=PRO_0000005973"
                     /note="propagated from UniProtKB/Swiss-Prot (P01029.3)"
                     /calculated_mol_wt=72076
     Region          <136..1327
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Site            224
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P01029.3)"
     mat_peptide     678..1443
                     /product="Complement C4 alpha chain. /id=PRO_0000005975"
                     /note="propagated from UniProtKB/Swiss-Prot (P01029.3)"
                     /calculated_mol_wt=84416
     mat_peptide     678..753
                     /product="C4a anaphylatoxin. /id=PRO_0000005976"
                     /note="propagated from UniProtKB/Swiss-Prot (P01029.3)"
                     /calculated_mol_wt=8718
     Site            743
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine; propagated from
                     UniProtKB/Swiss-Prot (P01029.3)"
     Region          779..867
                     /region_name="A2M"
                     /note="Alpha-2-macroglobulin family; pfam00207"
                     /db_xref="CDD:459711"
     Region          978..1311
                     /region_name="TED_complement"
                     /note="A-macroglobulin TED domain; pfam07678"
                     /db_xref="CDD:462227"
     Site            1324
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17330941; propagated from
                     UniProtKB/Swiss-Prot (P01029.3)"
     Site            1387
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P01029.3)"
     Site            1413
                     /site_type="sulfatation"
                     /note="Sulfotyrosine. /evidence=ECO:0000250; propagated
                     from UniProtKB/Swiss-Prot (P01029.3)"
     Site            1416
                     /site_type="sulfatation"
                     /note="Sulfotyrosine. /evidence=ECO:0000250; propagated
                     from UniProtKB/Swiss-Prot (P01029.3)"
     Site            1417
                     /site_type="sulfatation"
                     /note="Sulfotyrosine. /evidence=ECO:0000250; propagated
                     from UniProtKB/Swiss-Prot (P01029.3)"
     mat_peptide     1448..1738
                     /product="Complement C4 gamma chain. /id=PRO_0000005978"
                     /note="propagated from UniProtKB/Swiss-Prot (P01029.3)"
                     /calculated_mol_wt=33069
     Region          1475..1564
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     Region          1582..1736
                     /region_name="NTR_like"
                     /note="NTR_like domain; a beta barrel with an
                     oligosaccharide/oligonucleotide-binding fold found in
                     netrins, complement proteins, tissue inhibitors of
                     metalloproteases (TIMP), and procollagen C-proteinase
                     enhancers (PCOLCE), amongst others. In netrins, the...;
                     cl02512"
                     /db_xref="CDD:470599"
     CDS             1..1738
                     /gene="C4b"
                     /gene_synonym="C4; Ss"
                     /coded_by="NM_009780.2:72..5288"
                     /db_xref="CCDS:CCDS28657.1"
                     /db_xref="GeneID:12268"
                     /db_xref="MGI:MGI:88228"
ORIGIN      
        1 mrllwglawv fsfcasslqk prlllfspsv vnlgtplsvg vqlldappgq evkgsvflrn
       61 pkggscspkk dfklssgddf vllslevple dvrscglfdl rraphiqlva qspwlrntaf
      121 katetqgvnl lfssrrghif vqtdqpiynp gqrvryrvfa ldqkmrpstd fltitvensh
      181 glrvlkkeif tstsifqdaf tipdisepgt wkisarfsdg lesnrsthfe vkkyvlpnfe
      241 vkitpwkpyi lmvpsnsdei qldiqaryiy gkpvqgvayt rfalmdeqgk rtflrgletq
      301 aklvegrthi siskdqfqaa ldkinigvrd leglrlyaat aviespggem eeaeltswrf
      361 vssafsldls rtkrhlvpga hfllqalvqe msgseasnvp vkvsatlvsg sdsqvldiqq
      421 stngigqvsi sfpipptvte lrllvsagsl ypaiarltvq appsrgtgfl siepldprsp
      481 svgdtfilnl qpvgipaptf shyyymiisr gqimamgrep rktvtsvsvl vdhqlapsfy
      541 fvayfyhqgh pvansllini qsrdcegklq lkvdgakeyr nadmmklriq tdskalvalg
      601 avdmalyavg grshkpldms kvfevinsyn vgcgpgggdd alqvfqdagl afsdgdrltq
      661 tredlscpke kksrqkrnvn fqkavseklg qysspdakrc cqdgmtklpm krtceqraar
      721 vpqqacrepf lscckfaedl rrnqtrsqah larnnhnmlq eedlideddi lvrtsfpenw
      781 lwrvepvdss klltvwlpds mttweihgvs lskskglcva kptrvrvfrk fhlhlrlpis
      841 irrfeqfelr pvlynylndd vavsvhvtpv eglclagggm maqqvtvpag sarpvafsvv
      901 ptaaanvplk vvargvfdlg davskilqie kegaihreel vynldplnnl grtleipgss
      961 dpnivpdgdf sslvrvtase pletmgsega lspggvasll rlpqgcaeqt miylaptlta
     1021 snyldrteqw sklspetkdh avdliqkgym riqqfrkndg sfgawlhrds stwltafvlk
     1081 ilslaqeqvg nspeklqeta swllaqqlgd gsfhdpcpvi hramqgglvg sdetvaltaf
     1141 vvialhhgld vfqdddakql knrveasitk ansflgqkas agllgahaaa itayaltltk
     1201 asedlrnvah nslmamaeet gehlywglvl gsqdkvvlrp taprsptepv pqapalwiet
     1261 tayallhlll regkgkmadk aaswlthqgs fhgafrstqd tvvtldalsa ywiashttee
     1321 kalnvtlssm grnglkthgl hlnnhqvkgl eeelkfslgs tisvkvegns kgtlkilrty
     1381 nvldmknttc qdlqievkvt gaveyawdan edyedyydmp aaddpsvplq pvtplqlfeg
     1441 rrsrrrreap kvveeqesrv qytvciwrng klglsgmaia ditllsgfha lradleklts
     1501 lsdryvshfe tdgphvllyf dsvpttrecv gfgasqevvv glvqpssavl ydyyspdhkc
     1561 svfyaaptks qllatlcsgd vcqcaegkcp rllrslerrv edkdgyrmrf acyyprveyg
     1621 ftvkvlredg raafrlfesk itqvlhfrkd tmasigqtrn flsrascrlr lepnkeylim
     1681 gmdgetsdnk gdpqylldsn twieempseq mckstrhraa cfqlkdflme fssrgcqv
//
