LOCUS       NM_001310771             873 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus BUD31 homolog (Bud31), transcript variant 2, mRNA.
ACCESSION   NM_001310771 XM_006504827
VERSION     NM_001310771.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Qin,J., Huang,T., Wang,Z., Zhang,X., Wang,J., Dang,Q., Cui,D.,
            Wang,X., Zhai,Y., Zhao,L., Lu,G., Shao,C., Li,S., Liu,H. and Liu,Z.
  TITLE     Bud31-mediated alternative splicing is required for spermatogonial
            stem cell self-renewal and differentiation
  JOURNAL   Cell Death Differ 30 (1), 184-194 (2023)
   PUBMED   36114296
  REMARK    GeneRIF: Bud31-mediated alternative splicing is required for
            spermatogonial stem cell self-renewal and differentiation.
REFERENCE   2  (bases 1 to 873)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AA051275.1, CJ047068.1, CJ044770.1, CX567360.1 and BP770669.1.
            
            On Jul 18, 2015 this sequence version replaced XM_006504827.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BU526016.1, BI558275.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AA051275.1         1-24
            25-30               CJ047068.1         6-11
            31-446              CJ044770.1         2-417
            447-764             CX567360.1         404-721
            765-873             BP770669.1         27-135              c
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 85.05 cM"
     gene            1..873
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /note="BUD31 homolog"
                     /db_xref="GeneID:231889"
                     /db_xref="MGI:MGI:2141291"
     exon            1..67
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /inference="alignment:Splign:2.1.0"
     exon            68..199
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /inference="alignment:Splign:2.1.0"
     exon            200..319
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..660
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /note="isoform 1 is encoded by transcript variant 2;
                     protein BUD31 homolog; protein G10 homolog; maternal G10
                     transcript"
                     /codon_start=1
                     /product="protein BUD31 homolog isoform 1"
                     /protein_id="NP_001297700.1"
                     /db_xref="CCDS:CCDS80463.1"
                     /db_xref="GeneID:231889"
                     /db_xref="MGI:MGI:2141291"
                     /translation="MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVE
                     SLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCC
                     LRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG"
     exon            320..442
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /inference="alignment:Splign:2.1.0"
     exon            443..609
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /inference="alignment:Splign:2.1.0"
     exon            610..853
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      828..833
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /note="hexamer: AATAAA"
     polyA_site      849
                     /gene="Bud31"
                     /gene_synonym="EDG-2; EDG2; G10"
                     /note="major polyA site"
ORIGIN      
        1 gaagagtttg tcgccggagc ggaagccgtc aataaagaag cagcggcctt aggtgcttga
       61 gtttccgatg agacagaaga ctgcagaaga aaagtgatct cccccagagt cacagaatag
      121 ttggtaaatc tggacaagaa cacagctttg taactgtgaa gaccaagaat tttttttata
      181 ttactctcaa tacccaaaga tctttataag ctatcttgct gggaaatgcc caaggtcaaa
      241 agaagccgga aagcacctcc agatggctgg gagttgattg aaccaacgtt ggatgaacta
      301 gatcaaaaga tgagagaagc tgaaactgaa ccccatgagg gaaagaggaa agtggaatct
      361 ctgtggccca tcttcaggat ccatcaccag aaaacccgct atatcttcga cctcttttac
      421 aagaggaaag ccataagcag agaactctat gaatactgca ttaaagaagg ctatgcagac
      481 aaaaacctga ttgcaaagtg gaagaagcag ggctatgaga acctatgctg cctacgctgc
      541 attcagactc gggacaccaa ctttggcacc aactgcattt gccgggttcc caaaagcaag
      601 ctggaagtgg gtcgcatcat cgagtgcaca cactgtggct gccggggttg ctctggctga
      661 cacccttgac tcctctgcat cctggtcttt ggaccttgga cttaagactt attgggccac
      721 tgcctgaagc aactcttctc agagtggtgc tcagagaaaa gacccagggg gggaagcatg
      781 agctgtacag gtgacgactt tagtgttcac cagctgagat ctgtaaaaat aaaaccttta
      841 gacctcctcg ctaaaaaaaa aaaaaaaaaa aaa
//
