LOCUS       NM_001317350            2154 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus BUB3 mitotic checkpoint protein (Bub3), transcript
            variant 2, mRNA.
ACCESSION   NM_001317350 XM_006507270
VERSION     NM_001317350.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Yue,W., Wang,Y., Meng,T.G., Zhang,H.Y., Zhang,X.R., Ouyang,Y.C.,
            Hou,Y., Schatten,H., Wang,Z.B. and Sun,Q.Y.
  TITLE     Kinetochore scaffold 1 regulates SAC function during mouse oocyte
            meiotic maturation
  JOURNAL   FASEB J 36 (3), e22210 (2022)
   PUBMED   35167144
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Dai,X.X., Xiong,H., Zhang,M., Sun,S. and Xiong,B.
  TITLE     Zfp207 is a Bub3 binding protein regulating meiotic chromosome
            alignment in mouse oocytes
  JOURNAL   Oncotarget 7 (21), 30155-30165 (2016)
   PUBMED   27177335
  REMARK    GeneRIF: Data show that Zfp207 binds to Bub3 to form a complex and
            maintains its protein level in oocytes, and that overexpression of
            Bub3 is able to partially rescue the occurrence of aneuploid eggs
            in Zfp207-depleted oocytes.
REFERENCE   3  (bases 1 to 2154)
  AUTHORS   Toledo,C.M., Herman,J.A., Olsen,J.B., Ding,Y., Corrin,P.,
            Girard,E.J., Olson,J.M., Emili,A., DeLuca,J.G. and Paddison,P.J.
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and
            chromosome alignment
  JOURNAL   Dev Cell 28 (3), 282-294 (2014)
   PUBMED   24462187
REFERENCE   4  (bases 1 to 2154)
  AUTHORS   Jiang,H., He,X., Wang,S., Jia,J., Wan,Y., Wang,Y., Zeng,R.,
            Yates,J. 3rd, Zhu,X. and Zheng,Y.
  TITLE     A microtubule-associated zinc finger protein, BuGZ, regulates
            mitotic chromosome alignment by ensuring Bub3 stability and
            kinetochore targeting
  JOURNAL   Dev Cell 28 (3), 268-281 (2014)
   PUBMED   24462186
REFERENCE   5  (bases 1 to 2154)
  AUTHORS   Wei,Y., Multi,S., Yang,C.R., Ma,J., Zhang,Q.H., Wang,Z.B., Li,M.,
            Wei,L., Ge,Z.J., Zhang,C.H., Ouyang,Y.C., Hou,Y., Schatten,H. and
            Sun,Q.Y.
  TITLE     Spindle assembly checkpoint regulates mitotic cell cycle
            progression during preimplantation embryo development
  JOURNAL   PLoS One 6 (6), e21557 (2011)
   PUBMED   21720555
REFERENCE   6  (bases 1 to 2154)
  AUTHORS   Saxena,A., Saffery,R., Wong,L.H., Kalitsis,P. and Choo,K.H.
  TITLE     Centromere proteins Cenpa, Cenpb, and Bub3 interact with
            poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated
  JOURNAL   J Biol Chem 277 (30), 26921-26926 (2002)
   PUBMED   12011073
  REMARK    GeneRIF: Cenpa, Cenpb, and Bub3, but not Cenpc, interacted with
            PARP-1
REFERENCE   7  (bases 1 to 2154)
  AUTHORS   Sudakin,V., Chan,G.K. and Yen,T.J.
  TITLE     Checkpoint inhibition of the APC/C in HeLa cells is mediated by a
            complex of BUBR1, BUB3, CDC20, and MAD2
  JOURNAL   J Cell Biol 154 (5), 925-936 (2001)
   PUBMED   11535616
REFERENCE   8  (bases 1 to 2154)
  AUTHORS   Kalitsis,P., Earle,E., Fowler,K.J. and Choo,K.H.
  TITLE     Bub3 gene disruption in mice reveals essential mitotic spindle
            checkpoint function during early embryogenesis
  JOURNAL   Genes Dev 14 (18), 2277-2282 (2000)
   PUBMED   10995385
REFERENCE   9  (bases 1 to 2154)
  AUTHORS   Martinez-Exposito,M.J., Kaplan,K.B., Copeland,J. and Sorger,P.K.
  TITLE     Retention of the BUB3 checkpoint protein on lagging chromosomes
  JOURNAL   Proc Natl Acad Sci U S A 96 (15), 8493-8498 (1999)
   PUBMED   10411903
REFERENCE   10 (bases 1 to 2154)
  AUTHORS   Fowler,K.J., Kalitsis,P. and Choo,K.H.
  TITLE     Mouse mitotic spindle checkpoint Bub3 gene maps to the distal
            region of chromosome 7 by interspecific backcross analysis
  JOURNAL   Cytogenet Cell Genet 87 (1-2), 91-92 (1999)
   PUBMED   10640819
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159994.7.
            
            On Sep 9, 2022 this sequence version replaced NM_001317350.1.
            
            Summary: This gene encodes a component of the mitotic spindle
            assembly complex. The encoded protein is involved in the regulation
            of chromosome segregation during oocyte meiosis. Disruption of this
            gene results in the accumulation of mitotic errors and is lethal in
            the embryonic stage. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.2854613.1,
                                           SRR13422598.1077493.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               AC159994.7         109968-110132
            166-360             AC159994.7         110307-110501
            361-430             AC159994.7         111134-111203
            431-582             AC159994.7         112931-113082
            583-741             AC159994.7         115790-115948
            742-919             AC159994.7         117781-117958
            920-1136            AC159994.7         118102-118318
            1137-2154           AC159994.7         120476-121493
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 74.36 cM"
     gene            1..2154
                     /gene="Bub3"
                     /note="BUB3 mitotic checkpoint protein"
                     /db_xref="GeneID:12237"
                     /db_xref="MGI:MGI:1343463"
     exon            1..165
                     /gene="Bub3"
                     /inference="alignment:Splign:2.1.0"
     CDS             166..1146
                     /gene="Bub3"
                     /note="isoform a is encoded by transcript variant 2;
                     mitotic checkpoint protein BUB3; WD repeat type I
                     transmembrane protein A72.5; budding uninhibited by
                     benzimidazoles 3 homolog"
                     /codon_start=1
                     /product="mitotic checkpoint protein BUB3 isoform a"
                     /protein_id="NP_001304279.1"
                     /db_xref="CCDS:CCDS40160.1"
                     /db_xref="GeneID:12237"
                     /db_xref="MGI:MGI:1343463"
                     /translation="MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDV
                     PANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCV
                     EYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVL
                     VWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKK
                     YAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYP
                     TSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKST"
     exon            166..360
                     /gene="Bub3"
                     /inference="alignment:Splign:2.1.0"
     exon            361..430
                     /gene="Bub3"
                     /inference="alignment:Splign:2.1.0"
     exon            431..582
                     /gene="Bub3"
                     /inference="alignment:Splign:2.1.0"
     exon            583..741
                     /gene="Bub3"
                     /inference="alignment:Splign:2.1.0"
     exon            742..919
                     /gene="Bub3"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1136
                     /gene="Bub3"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..2154
                     /gene="Bub3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1407..1412
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bub3"
                     /note="hexamer: AATAAA"
     polyA_site      1435
                     /gene="Bub3"
                     /note="major polyA site"
     regulatory      2129..2134
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bub3"
                     /note="hexamer: ATTAAA"
     polyA_site      2154
                     /gene="Bub3"
ORIGIN      
        1 gccttctccg ctttgcctgc gagtgctcgg agggaagcgg atcggtagtg gccggtgcag
       61 aaacgttgct taggcggaca ggaggtagag gttcgggcga gggcgggcgg agcgggatct
      121 gtggcccgtg gccggcgcat cgatgcacag agggatccgg tcgagatgac cggttcgaac
      181 gaattcaagc tgaaccagcc acccgaggat ggcatctcct cggttaagtt cagccccaac
      241 acatcccagt tcctcctggt ctcctcctgg gatacgtctg tgcgcctcta cgatgtgccc
      301 gccaattcca tgaggctcaa gtaccagcac actggtgctg tcctggactg cgccttctac
      361 gatccaacgc atgcctggag tggtggatta gatcatcaac tgaaaatgca cgatttgaac
      421 actgatcaag aaaatcttgt tggaactcac gatgccccta tcagatgtgt tgaatactgt
      481 ccagaagtga acgtgatggt aactggaagt tgggatcaga cagttaaact gtgggacccc
      541 agaactccgt gtaatgctgg gactttctcc cagccggaaa aggtctacac cctgtcagtg
      601 tctggggaca ggctgattgt gggcacggcg ggccgccgag tgctggtgtg ggacttgcgg
      661 aacatgggct atgtgcagca gcggagggag tccagcctga agtaccagac tcgctgcatc
      721 cgagccttcc cgaacaagca gggttatgtg ttgagctcca tcgaaggccg agtggctgtg
      781 gaatacttgg acccgagccc tgaggtgcag aagaagaagt acgccttcaa gtgccacagg
      841 ctaaaggaga acaacattga gcagatttac ccagtcaacg ccatctcctt tcacaacatc
      901 cacaacacgt ttgccacagg tggttcggat ggattcgtca atatttggga cccatttaac
      961 aagaagcgcc tgtgccagtt ccatcggtac cccaccagca tcgcttccct tgccttcagt
     1021 aatgacggaa ctacgcttgc aatagcatcg tcatatatgt atgagatgga tgacaccgag
     1081 catcctgaag atggtatctt cattcgccaa gtgacagacg cagaaacaaa gcccaagtcc
     1141 acgtaatctg gggaaagcag tttctctgga aaagctttgc ttcctacaaa cacgcgcttt
     1201 ccctgaggcc tgcttgcagc cattggacac ttggtctagt gtgtggattt ctatgtcttc
     1261 tgtcgtaaca gaattgtctc tgtgccctgt gattgttttg ggtagggacg ctttgtatcc
     1321 ttgtacagta gtcttgacga ttctcctccc ctacgccatt tttgtttgtg tttgctatct
     1381 aaaactgtta tatttttctg ttgtctaata aatcagagat ttggaacttt taaaagttgg
     1441 gtgtttgtaa acattttggt agtgtcattt attgcataac aaggcttttc ttaggcaaag
     1501 tactctttgt cattagaggt gtcatttgac tgaatgtacc atgccttatt tattatatct
     1561 gctcttgtac cagggggtca gccctgggta aaggaatcca cttgacttct ttcagccagt
     1621 ttttgctcct gtttctttgg aggctcagtg aggcctggga gccagctctg cagcttctca
     1681 agatgggcac tgaccggcag catctgcgtg gctgctagca gtgccagtgt gggtagggct
     1741 catgcttccg tgggctgacg ataccatcct ctgtagacag agccaaaggg caggcccctc
     1801 tggacttagc catcagctgc tatgtcactg aagccctccg gacgataaat ggtgtaccag
     1861 ccaggagcta ctggtgggat tggtcttgtg gtgtagtcgt atgcttttta aagtcataac
     1921 cacgtgagca taaatttcaa acattgtata acttaaaaag agaatgggcc ttgttaaagg
     1981 tgaccaaatt aaagccgtgt tatggacttg tgtttgaagt ggtatagaga gctgctgatg
     2041 gttccgtttg gctgtccctc tgtccctaaa tgtgtctcct accccttgtg tgccacctgt
     2101 gcagctcacc ctctcaattt gcctgtttat taaagttacc tttgctgcca gttt
//
