LOCUS       NM_009772               4334 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus BUB1, mitotic checkpoint serine/threonine kinase
            (Bub1), transcript variant 2, mRNA.
ACCESSION   NM_009772
VERSION     NM_009772.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Yoshida,S., Ikedo,A., Yanagihara,Y., Sakaue,T., Saeki,N. and
            Imai,Y.
  TITLE     Bub1 suppresses inflammatory arthritis-associated bone loss in mice
            through inhibition of TNFalpha-mediated osteoclastogenesis
  JOURNAL   J Bone Miner Res 39 (3), 341-356 (2024)
   PUBMED   38477771
  REMARK    GeneRIF: Bub1 suppresses inflammatory arthritis-associated bone
            loss in mice through inhibition of TNFalpha-mediated
            osteoclastogenesis.
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4334)
  AUTHORS   Taliani,V., Buonaiuto,G., Desideri,F., Setti,A., Santini,T.,
            Galfre,S., Schirone,L., Mariani,D., Frati,G., Valenti,V.,
            Sciarretta,S., Perlas,E., Nicoletti,C., Musaro,A. and Ballarino,M.
  TITLE     The long noncoding RNA Charme supervises cardiomyocyte maturation
            by controlling cell differentiation programs in the developing
            heart
  JOURNAL   Elife 12, e81360 (2023)
   PUBMED   36877136
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4334)
  AUTHORS   Chong,D., Gu,Y., Zhang,T., Xu,Y., Bu,D., Chen,Z., Xu,N., Li,L.,
            Zhu,X., Wang,H., Li,Y., Zheng,F., Wang,D., Li,P., Xu,L., Hu,Z. and
            Li,C.
  TITLE     Neonatal ketone body elevation regulates postnatal heart
            development by promoting cardiomyocyte mitochondrial maturation and
            metabolic reprogramming
  JOURNAL   Cell Discov 8 (1), 106 (2022)
   PUBMED   36220812
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4334)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4334)
  AUTHORS   Pangilinan,F., Li,Q., Weaver,T., Lewis,B.C., Dang,C.V. and
            Spencer,F.
  TITLE     Mammalian BUB1 protein kinases: map positions and in vivo
            expression
  JOURNAL   Genomics 46 (3), 379-388 (1997)
   PUBMED   9441741
REFERENCE   7  (bases 1 to 4334)
  AUTHORS   Taylor,S.S. and McKeon,F.
  TITLE     Kinetochore localization of murine Bub1 is required for normal
            mitotic timing and checkpoint response to spindle damage
  JOURNAL   Cell 89 (5), 727-735 (1997)
   PUBMED   9182760
REFERENCE   8  (bases 1 to 4334)
  AUTHORS   Kerr,S.M., Taggart,M.H., Lee,M. and Cooke,H.J.
  TITLE     Ott, a mouse X-linked multigene family expressed specifically
            during meiosis
  JOURNAL   Hum Mol Genet 5 (8), 1139-1148 (1996)
   PUBMED   8842733
REFERENCE   9  (bases 1 to 4334)
  AUTHORS   Bassett,D.E. Jr., Boguski,M.S., Spencer,F., Reeves,R., Goebl,M. and
            Hieter,P.
  TITLE     Comparative genomics, genome cross-referencing and XREFdb
  JOURNAL   Trends Genet 11 (9), 372-373 (1995)
   PUBMED   7482790
REFERENCE   10 (bases 1 to 4334)
  AUTHORS   Kerr,S.M., Vambrie,S., McKay,S.J. and Cooke,H.J.
  TITLE     Analysis of cDNA sequences from mouse testis
  JOURNAL   Mamm Genome 5 (9), 557-565 (1994)
   PUBMED   8000140
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK042932.1, U89795.2 and AL844204.3.
            
            On Dec 27, 2007 this sequence version replaced NM_009772.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the mid-coding region, compared to variant 1,
            resulting in a shorter protein (isoform 2), compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK042932.1, U89795.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-108               AK042932.1         2-109
            109-3409            U89795.2           100-3400
            3410-4334           AL844204.3         149624-150548       c
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 62.1 cM"
     gene            1..4334
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /note="BUB1, mitotic checkpoint serine/threonine kinase"
                     /db_xref="GeneID:12235"
                     /db_xref="MGI:MGI:1100510"
     exon            1..140
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..3291
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     mitotic checkpoint serine/threonine-protein kinase BUB1;
                     mBUB1; budding uninhibited by benzimidazoles 1 homolog"
                     /codon_start=1
                     /product="mitotic checkpoint serine/threonine-protein
                     kinase BUB1 isoform 2"
                     /protein_id="NP_033902.2"
                     /db_xref="GeneID:12235"
                     /db_xref="MGI:MGI:1100510"
                     /translation="MDNLENVFRMFEAHMQSYTGNDPLGEWESFIKWVEENFPDNKEY
                     LMTLLEHLMKEFLHKKNYHNDSRFINYCLKFAEYNSDRHQFFEFLYNQGIGTKSSYIY
                     MSWAGHLEAQGELQHASAIFQTGIHNEAEPKELLQQQYRLFQARLTGIHLPAQATTSE
                     PLHSAQILNQVMMTNSSPEKNSACVPRSQGSECSGVASSTCDEKSNMEQRVIMISKSE
                     CSVSSSVAPKPEAQQVMYCKEKLIRGDSEFSFEELRAQKYNQRKKHEQWVSEDRNYMK
                     RKEANAFEEQLLKQKMDELHKKLHQVVELSHKDLPASENRPDVSLVCVGQNTCSQQEL
                     RGPSLSSISHQTSESSGEKPQEEPSVPLMVNAVNSTLLFPAANLPALPVPVSGQSLTD
                     SRCVNQSVHEFMPQCGPETKEVCETNKVASINDFHTTPNTSLGMVQGTPCKVQPSPTV
                     HTKEALGFIMDMFQAPTLPDISDDKDEWPSLDQNEDAFEAQFQKNAVSSGDWGVKKIM
                     TLSSAFPIFEDGNKENYGLPQPKNKPLGARTFGERSLSKYSSRSNEMPHTDEFMDDST
                     VCGIRCNKTLAPSPKSIGDFTSAAQLSSTPFHKFPADLVQIPEDKENVVATQYTHMAL
                     DSCKENIVDLSKGRKLGPIQEKISASLPCPSQPATGGLFTQEAVFGLEAFKCTGIDHA
                     TVEDLSDANAGLQVECVQTLGNVNAPSFTVENPWDDELILKLLSGLSKPVTSYSNTFE
                     WQSKLPAIKTKTEYQLGSLLVYVNHLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQR
                     PANSWEFYIGMQLMERLKPEVHHMFIKFYSAHLFKNGSILVGELYSYGTLLNVINLYK
                     NTSEKVMPQALVLTFAIRMLYMVEQVHSCEIIHGDIKPDNFILGHRFLEQADEDLATG
                     LALIDLGQSIDMKLFPKGTVFTGKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCML
                     FGSYMKVKNEGGVWKPEGLFRRLPHLDMWEEFFHIMLNIPDCHNLPSLDFLRQNMKKL
                     LEQQYSNKIKTLRNRLIVMLSEYKRSRK"
     exon            141..200
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            201..339
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            340..536
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            537..580
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            581..681
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            682..734
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            735..916
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1068
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1295
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1354
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1483
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1594
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1691
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1773
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1948
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2036
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2239
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            2240..2383
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2499
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2661
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..2819
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2988
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3095
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     exon            3096..4334
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3390..3395
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /note="hexamer: AATAAA"
     polyA_site      3413
                     /gene="Bub1"
                     /gene_synonym="Bub1a; D2Xrf87"
                     /note="major polyA site"
ORIGIN      
        1 ggctgcgcgg ggaattcgaa tcgccggcgg ccttcgagac tcggggttag gggctgatgg
       61 ggagtttggt cggcggctgg tggggtattt cgccgctgcg cgttttttct cgtcatggac
      121 aacctagaaa atgtctttcg catgtttgaa gcccatatgc aaagctacac gggtaatgac
      181 ccacttggag aatgggaaag ctttataaag tgggtagaag agaattttcc tgacaataaa
      241 gaatacttga tgacattatt agaacattta atgaaggaat ttttacataa gaagaactac
      301 cacaatgatt caagattcat caattattgc ttaaaatttg ctgagtacaa cagtgaccgt
      361 catcagtttt ttgagtttct gtacaaccag ggaattggaa ccaagtcatc atatatatac
      421 atgtcctggg cagggcatct ggaagcccag ggagagctgc agcatgccag tgctattttt
      481 cagacaggaa ttcacaatga ggctgaacct aaagaactac tacagcaaca atacaggcta
      541 ttccaagcac gccttactgg aatccatttg ccagctcaag ctacaacctc agaacctttg
      601 catagtgcac agattttaaa ccaagttatg atgacaaact caagtccaga aaaaaactca
      661 gcctgtgttc ctaggagtca gggttcagaa tgttctggtg tggcatcttc cacttgtgat
      721 gaaaagtcta atatggaaca aagggtgatc atgatttcca agtcagaatg ctctgtcagc
      781 tcatctgtgg cacccaagcc tgaggctcag caagttatgt actgcaagga aaagcttatt
      841 cgtggagatt cagaattttc ttttgaagaa ctgagagccc agaaatataa tcaaaggaag
      901 aagcatgagc agtgggttag tgaagacaga aattatatga aaaggaaaga agcaaatgct
      961 tttgaagagc aattattaaa acagaaaatg gatgaacttc acaagaaatt gcatcaagtg
     1021 gtggaattgt cacacaagga ccttcctgct tctgagaaca ggcctgatgt tagtctagta
     1081 tgtgttggac aaaatacttg ctcccagcag gaattgaggg gtccaagtct ttcatccatc
     1141 agtcatcaga cctcagagag ttcaggagag aaaccacagg aagaaccttc tgttcctctt
     1201 atggtaaatg ctgttaacag cactttgctg ttcccagctg ccaacctgcc agctcttcct
     1261 gttcctgtaa gtggccagtc attgacagac tccagatgtg tgaatcaaag tgttcatgaa
     1321 ttcatgccac agtgtggacc agaaacaaaa gaagtgtgtg aaacaaataa agttgccagc
     1381 attaatgatt ttcatacaac tccaaacaca tcattgggaa tggttcaagg aacaccatgc
     1441 aaagtgcagc catcaccaac tgtccacacc aaggaagcat taggtttcat catggacatg
     1501 tttcaggctc caacacttcc tgacatttct gatgataaag atgaatggcc atctctggac
     1561 caaaatgaag atgcatttga agcccagttt caaaaaaatg cagtatcttc gggagattgg
     1621 ggagttaaaa aaattatgac tttgtcatct gcttttccta tttttgaaga tggaaacaaa
     1681 gaaaattatg gcttaccaca gcctaaaaat aagcccttag gagctaggac ctttggagaa
     1741 cgatctctca gtaaatattc ctcgagatca aatgaaatgc ctcacactga tgagtttatg
     1801 gatgattcaa cagtatgtgg tattcgctgc aacaaaactc tagctcccag tcctaaaagt
     1861 ataggagact ttacatctgc tgcccaactt tcgtctacac cattccacaa atttccagca
     1921 gatttagtac agattccaga agataaagaa aatgtggtag ccacacagta tacacatatg
     1981 gctttggatt cttgtaaaga aaacatagtg gacctctcaa aaggcagaaa gcttgggcca
     2041 attcaagaga aaatttcagc atctttaccc tgtcctagtc agcctgccac aggtggtttg
     2101 ttcacccagg aagcagtgtt cggccttgag gcttttaaat gcacaggcat tgaccatgcg
     2161 acagtggaag acctatccga tgccaatgct gggctccaag ttgaatgcgt gcagacactt
     2221 ggaaatgtca atgctccaag ctttactgtt gagaacccat gggatgacga attgattctt
     2281 aaacttctct ctggactttc taagccagtt acttcctatt caaatacttt tgagtggcag
     2341 agtaaacttc cagccatcaa gaccaagaca gaatatcaat tgggttcttt gctggtctat
     2401 gtgaatcacc ttcttggaga aggagccttt gctcaagtct ttgaagctat tcatggagat
     2461 gtgagaaatg ccaaaagtga acagaaatgc attttgaagg tgcagagacc tgccaactcc
     2521 tgggaattct acattgggat gcagctgatg gaaagactaa agccagaagt acatcacatg
     2581 ttcatcaagt tttattctgc tcatttattc aagaatggca gcatattagt aggggaactc
     2641 tacagctatg ggacgttact aaatgtcatt aacctctata aaaatacctc tgaaaaagtg
     2701 atgccccagg ctcttgtcct cactttcgct atcagaatgc tttacatggt tgaacaagtc
     2761 cacagctgcg aaatcattca tggagacatt aagccagata acttcatact aggacacaga
     2821 tttttggaac aggctgatga agacttagct accggcttgg cattgattga cctgggtcag
     2881 agtatagata tgaaactttt ccctaaagga actgtattta caggaaaatg tgaaacatct
     2941 ggttttcagt gtcctgagat gctcagtaac aagccatgga actaccagat tgattacttt
     3001 ggagttgctg caacaatata ctgtatgctc tttggctctt acatgaaagt aaaaaatgaa
     3061 ggaggagtct ggaaacctga aggtcttttt agaaggcttc ctcatttgga tatgtgggag
     3121 gaattttttc acatcatgtt gaatataccg gattgtcata atcttccatc tttggatttt
     3181 ctgagacaga atatgaagaa attacttgaa caacagtatt ccaacaagat taagaccttg
     3241 cgtaataggc taattgtgat gctttcagaa tataagcgtt caagaaaata aaatgaatat
     3301 ggaccttctc caaaacacac ttagtaaatt taaactctca ctttgaatcc tatttttttc
     3361 atacttacta tttatgtatg tttaaaaaaa ataaagccca tatgatatac ccatgtgacc
     3421 tagttgttct aaatactcta acaaacaaag taaggagacc cctgtaagca ggctgatcta
     3481 ttgggttgct cttagtatac aaatcttctt gagaatgatt caaatttaga tgctttatag
     3541 aagttacctc cctgccccca actccaagac aatttctcag tataaccaga gccctggctg
     3601 tcctggactt ggctgtagac caggctggcc tcgagctcat agagaaccac ccgcctctgc
     3661 ctcccaagtg ctggggttac gggagtaacc accatgccct gctagaaatc acctttctaa
     3721 acactgacct atttaagtta aaagtaagcc ataaaaagag ttataaatta tgtttagtta
     3781 gcgtatgtag gattaggttt ccttatggca ttttaagaaa aaaaaatgtt tatttttctt
     3841 ccccatactt ttgaccccct ttgttctctt tcacacccca tagcttactt tccacttcca
     3901 tgtctcatct gtcaatgtcc cctcctcctt tcttaacatc tttttccctt aaggtcttta
     3961 ctttctaacc ccatgtattt ttatacaaat ttcatggtgt acatctgaaa agaattttat
     4021 tgtgtatgat tccacattgt cattatacaa ccatcagttg attgataaac atctgggctg
     4081 attccatttc tttgctattg tgaatagagg aacaattgac atggatgtgc aggtttctct
     4141 tgtaaaagat agaatccttt gtgtgccctg gagtaggaag ctagttcata aacagttcta
     4201 gttttctggg ggtttgtgtg tgtgtgtgtg tgtgtgttgt tttgttcttg ttgtgagacc
     4261 accatactga tttccacaat gaccatacta ggtcgcattt caactaacaa tgaataaaga
     4321 atttctgttt actc
//
