LOCUS       NM_175938               2983 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus butyrophilin, subfamily 2, member A2 (Btn2a2),
            transcript variant 1, mRNA.
ACCESSION   NM_175938
VERSION     NM_175938.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Frech,M., Danzer,H., Uchil,P., Azizov,V., Schmid,E., Schalter,F.,
            Durholz,K., Mauro,D., Rauber,S., Munoz,L., Taher,L., Ciccia,F.,
            Schober,K., Irla,M., Sarter,K., Schett,G. and Zaiss,M.M.
  TITLE     Butyrophilin 2a2 (Btn2a2) expression on thymic epithelial cells
            promotes central T cell tolerance and prevents autoimmune disease
  JOURNAL   J Autoimmun 139, 103071 (2023)
   PUBMED   37356345
  REMARK    GeneRIF: Butyrophilin 2a2 (Btn2a2) expression on thymic epithelial
            cells promotes central T cell tolerance and prevents autoimmune
            disease.
REFERENCE   3  (bases 1 to 2983)
  AUTHORS   Frech,M., Omata,Y., Schmalzl,A., Wirtz,S., Taher,L., Schett,G.,
            Zaiss,M.M. and Sarter,K.
  TITLE     Btn2a2 Regulates ILC2-T Cell Cross Talk in Type 2 Immune Responses
  JOURNAL   Front Immunol 13, 757436 (2022)
   PUBMED   35145516
  REMARK    GeneRIF: Btn2a2 Regulates ILC2-T Cell Cross Talk in Type 2 Immune
            Responses.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2983)
  AUTHORS   Frech,M., Schuster,G., Andes,F.T., Schett,G., Zaiss,M.M. and
            Sarter,K.
  TITLE     RANKL-Induced Btn2a2 - A T Cell Immunomodulatory Molecule - During
            Osteoclast Differentiation Fine-Tunes Bone Resorption
  JOURNAL   Front Endocrinol (Lausanne) 12, 685060 (2021)
   PUBMED   34421818
  REMARK    GeneRIF: RANKL-Induced Btn2a2 - A T Cell Immunomodulatory Molecule
            - During Osteoclast Differentiation Fine-Tunes Bone Resorption.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2983)
  AUTHORS   Sarter,K., Leimgruber,E., Gobet,F., Agrawal,V., Dunand-Sauthier,I.,
            Barras,E., Mastelic-Gavillet,B., Kamath,A., Fontannaz,P., Guery,L.,
            Duraes Fdo,V., Lippens,C., Ravn,U., Santiago-Raber,M.L.,
            Magistrelli,G., Fischer,N., Siegrist,C.A., Hugues,S. and Reith,W.
  TITLE     Btn2a2, a T cell immunomodulatory molecule coregulated with MHC
            class II genes
  JOURNAL   J Exp Med 213 (2), 177-187 (2016)
   PUBMED   26809444
  REMARK    GeneRIF: Btn2a2(-/-) mice exhibited enhanced effector CD4(+) and
            CD8(+) T cell responses, impaired CD4(+) regulatory T cell
            induction, potentiated antitumor responses, and exacerbated
            experimental autoimmune encephalomyelitis.
REFERENCE   6  (bases 1 to 2983)
  AUTHORS   Ammann,J.U., Cooke,A. and Trowsdale,J.
  TITLE     Butyrophilin Btn2a2 inhibits TCR activation and
            phosphatidylinositol 3-kinase/Akt pathway signaling and induces
            Foxp3 expression in T lymphocytes
  JOURNAL   J Immunol 190 (10), 5030-5036 (2013)
   PUBMED   23589618
REFERENCE   7  (bases 1 to 2983)
  AUTHORS   Smith,I.A., Knezevic,B.R., Ammann,J.U., Rhodes,D.A., Aw,D.,
            Palmer,D.B., Mather,I.H. and Trowsdale,J.
  TITLE     BTN1A1, the mammary gland butyrophilin, and BTN2A2 are both
            inhibitors of T cell activation
  JOURNAL   J Immunol 184 (7), 3514-3525 (2010)
   PUBMED   20208008
  REMARK    GeneRIF: These data are consistent with a coinhibitory role for
            mouse Butyrophilins
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ065534.1, AK051061.1 and AL714025.12.
            
            On Apr 11, 2007 this sequence version replaced NM_175938.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1). Variants 1 and 3
            encode the same isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051061.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164132, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                CJ065534.1         1-15
            16-561              AK051061.1         2-547
            562-1563            AK051061.1         549-1550
            1564-1564           AL714025.12        82407-82407         c
            1565-1803           AK051061.1         1552-1790
            1804-2983           AL714025.12        80988-82167         c
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 9.74 cM"
     gene            1..2983
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /note="butyrophilin, subfamily 2, member A2"
                     /db_xref="GeneID:238555"
                     /db_xref="MGI:MGI:3606486"
     exon            1..222
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /inference="alignment:Splign:2.1.0"
     exon            223..352
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..1812
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; butyrophilin 2"
                     /codon_start=1
                     /product="butyrophilin subfamily 2 member A2 isoform 1
                     precursor"
                     /protein_id="NP_787952.2"
                     /db_xref="CCDS:CCDS36619.1"
                     /db_xref="GeneID:238555"
                     /db_xref="MGI:MGI:3606486"
                     /translation="MEPTTSLRSCPIASLLFFLVLSLFVLVSAQFTVIGPAEPILAMV
                     GENTTLHCHLSPERNAEEMEVRWFRWRFFPAVLVYRGHQERPEEQMVAYRGRTTFMRT
                     DISKGRVALIIHNVTAYDNGIYCCYFQEGRSYDQATMKLMVASLGSEPLIKMKTLEDG
                     SILLECTSEGWYPEPRAVWRDPYDEVVPALEEEYTADREGLFTVTMTIIIRDCSVRNM
                     TCSVNNTLLSQEVESVILIPESFVPSLPLWMVAVAVTLPVVMLILLTSGSICLVKKHR
                     RKKSILSAEKEAEYEEKEAARQLQEELRWRRTLLHAADVVLDPDTAHPELFLSDDQRS
                     VIRGSSRQSVPDNPERFDCRPCVLGRESFSSGKHYWEVEVENVMVWAIGVCRDSVERK
                     GEALLVPQNGFWTLEMFGSQYRALSSPEKIIPLKERLHRIAVFLDCEGGDISFYNMRD
                     RSHIYTCPPVTFTGPLRPFFRLGSDDSPLFICPAFTGAQGVTIPEGGLFLYKTRPISQ
                     SLVRKP"
     exon            353..700
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /inference="alignment:Splign:2.1.0"
     exon            701..982
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1156
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1177
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1204
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..2983
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2818..2823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /note="hexamer: ATTAAA"
     polyA_site      2837
                     /gene="Btn2a2"
                     /gene_synonym="Btn2; D030063K05"
                     /note="major polyA site"
ORIGIN      
        1 gaaattgtga gacttgcacg cggaatgggt cctccgaggt ctgctgtcgc gagtcccagc
       61 actttgcaag taatggagaa cagaaaattc tttcctctct actgtagcag tttgttctct
      121 ggtggcgact gtgctcagcg acaagttgga gagtagagaa aaggcaagat aatcagcatt
      181 tgagggtcag agaagaaaag aaaacgcagt taattctaga aggttttctg tccacacgtg
      241 acctaggtga ctctgtcctg aagacctatg gagcctacaa cttccctgcg ttcttgcccg
      301 atagcctccc ttctcttctt cttggtcctc agcctgtttg tgctggtctc agcccagttt
      361 actgtcatag gaccagctga gcccatcctg gccatggtag gagagaatac cacactacac
      421 tgccacctgt caccagagag aaatgccgaa gagatggagg tgcggtggtt ccggtggcgt
      481 ttcttccctg cagtgctggt gtacagaggc catcaagaga gaccagagga gcagatggtg
      541 gcataccgag gaagaaccac cttcatgcgc acagacatca gcaagggaag agttgcgctc
      601 attatccaca atgtcacagc ctatgacaat ggcatctact gctgttactt ccaggaaggc
      661 aggtcctatg accaggcaac catgaagctt atggtggcaa gccttggctc tgagccactt
      721 attaaaatga agacacttga ggatgggagc atcttgctag agtgcacatc tgaagggtgg
      781 tacccagagc cccgagctgt gtggagagac ccctatgatg aagttgtacc tgccctggag
      841 gaggagtata cagctgacag agaaggcctc ttcacagtca ccatgactat aatcatcagg
      901 gactgctctg tgaggaacat gacctgctct gtcaataaca ctctgctcag ccaggaggtg
      961 gaaagtgtga ttctcattcc agaatccttc gtgcccagcc ttcctctgtg gatggtggct
     1021 gtggctgtca ctctgcctgt agtaatgctg attctcctca catctggaag catctgcctt
     1081 gtcaagaaac accgcaggaa gaaatctatt ctgtcagctg aaaaagaagc cgaatatgaa
     1141 gagaaggaag ctgcacggca acttcaagag gaactgcgat ggagacgaac cctcttacat
     1201 gctgctgacg tggtcctgga cccagataca gctcatcctg agctcttcct gtcagatgac
     1261 cagagaagtg taatacgagg ctcttcgagg cagagtgtgc ctgacaaccc tgagagattt
     1321 gactgccgtc catgtgtcct gggcagggaa agcttctcct cagggaagca ttactgggag
     1381 gtggaggtgg aaaatgtaat ggtgtgggcc attggtgttt gtagagacag cgtggaaagg
     1441 aaaggggagg ccctgttggt tcctcagaat ggcttctgga ccctggagat gtttggaagc
     1501 cagtatcgag ccctgtcctc cccagaaaag atcatacctc tgaaagagcg tcttcaccgt
     1561 atagctgtct tcctggactg tgagggtgga gatatttctt tctacaacat gagagacaga
     1621 tcacacattt acacatgtcc tcctgtgact ttcactgggc ccctgagacc cttctttagg
     1681 cttggttctg atgacagtcc cctgttcatc tgtccagcat tcacaggggc acagggagtt
     1741 acaatacctg agggtggctt attcctatat aagacaagac caatttctca gagccttgta
     1801 aggaagccat agctctctac acagtaccat ctgttggaga ctagacccca tgtccttcag
     1861 atcacatgga gcatcttcca gctgccacct tcacacatac ttcaggccca gtcctcagat
     1921 tactacatca tttcttctaa ctatgggcct aggtagagcc agtcttaggg gactattgct
     1981 gtaatacagc tctctcctga gaagaaagtg tgagaagggc agaaaacttg gagtttcaac
     2041 atgctgctct ggtcacagtg gatatcaggc aagagcaaca gggtggatca ggatgtaaga
     2101 agtgagaact acagaggaag gagacagata aagatgaatt gaggccgaag atggaggaaa
     2161 tggactgaag agctctgggg taagccctat gtgacagctg tggataggta ggagctaatg
     2221 gtccattgat atccaaagcc aaagatttaa atatcacata gtgtgtctgg agtgtatatc
     2281 tgtagaccta cacatgagag gaaacaatca tagtgatgaa ctggatgtaa gctggctcag
     2341 acgtccctac aataaacact tctgagttcc atgtctgtgc tcagtaagaa tggcttgagg
     2401 cttgcggtcc atgctgagca gccaggtcca catgaatcgg atttactaga gtaggtagca
     2461 gttcaagttc cttaggctca ggatgtcttc ctttccccca agcccttccc ccttcaagat
     2521 aggtctcact atgtagacca ggccagcctc cacctccaga gttctgggat taaagacaag
     2581 cacaaccatg tccagtttat gagcttgtga tatatacaga agattaagtt ctgtgttctt
     2641 gggttagtaa ctgttgagat ttgttttgag tcatgctctc actggctagc actgctcttg
     2701 actttctctc cccatctttt tgttattgct tttcaagaca tggtttcact gtgtatttct
     2761 ggctgataag ctgattttga attcacagag atctgcctct gcctcctgag tgctgggatt
     2821 aaaggtgtgt tacactacgc ctggcttcac tctatctctt cagtgtgggg attataggtt
     2881 tatactatca tgcctaacta atgtctgttg ctgcatatga catttgaact ttagaacaga
     2941 aaaacaacta tacatattaa tatatattaa actaataata agc
//
