LOCUS       NM_001378266            7062 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus BTB domain containing 8 (Btbd8), transcript variant 1,
            mRNA.
ACCESSION   NM_001378266
VERSION     NM_001378266.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Yang,X., He,Z., Dong,Q., Nai,S., Duan,X., Yu,J., Zhao,N., Du,X. and
            Chen,L.
  TITLE     Btbd8 deficiency reduces susceptibility to colitis by enhancing
            intestinal barrier function and suppressing inflammation
  JOURNAL   Front Immunol 15, 1382661 (2024)
   PUBMED   38558797
  REMARK    GeneRIF: Btbd8 deficiency reduces susceptibility to colitis by
            enhancing intestinal barrier function and suppressing inflammation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 7062)
  AUTHORS   Piccini,A., Castroflorio,E., Valente,P., Guarnieri,F.C., Aprile,D.,
            Michetti,C., Bramini,M., Giansante,G., Pinto,B., Savardi,A.,
            Cesca,F., Bachi,A., Cattaneo,A., Wren,J.D., Fassio,A., Valtorta,F.,
            Benfenati,F. and Giovedi,S.
  TITLE     APache Is an AP2-Interacting Protein Involved in Synaptic Vesicle
            Trafficking and Neuronal Development
  JOURNAL   Cell Rep 21 (12), 3596-3611 (2017)
   PUBMED   29262337
  REMARK    GeneRIF: APache is a presynaptic AP2 interactor on clathrin-coated
            vesicles.  APache silencing impairs clathrin-mediated endocytosis
            and synaptic function.
REFERENCE   4  (bases 1 to 7062)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC126598.13 and AC134411.7.
            
            On May 30, 2020 this sequence version replaced NM_001378266.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.7729.1,
                                           SRR10223971.6021.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               AC126598.13        138144-138359
            217-411             AC126598.13        146077-146271
            412-608             AC126598.13        152203-152399
            609-726             AC126598.13        161652-161769
            727-816             AC134411.7         51467-51556
            817-897             AC134411.7         65806-65886
            898-994             AC134411.7         66516-66612
            995-1083            AC134411.7         71422-71510
            1084-1186           AC134411.7         72238-72340
            1187-1369           AC134411.7         76630-76812
            1370-1507           AC134411.7         77495-77632
            1508-1637           AC134411.7         78480-78609
            1638-1699           AC134411.7         80786-80847
            1700-2375           AC134411.7         84098-84773
            2376-2466           AC134411.7         85263-85353
            2467-2606           AC134411.7         85750-85889
            2607-4853           AC134411.7         87635-89881
            4854-7062           AC134411.7         91111-93319
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 52.23 cM"
     gene            1..7062
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /note="BTB domain containing 8"
                     /db_xref="GeneID:100503185"
                     /db_xref="MGI:MGI:3646208"
     exon            1..216
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..5323
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /note="isoform 1 is encoded by transcript variant 1;
                     BTB/POZ domain-containing protein 8; APache; BTB (POZ)
                     domain containing 8; AP2-interacting clathrin-endocytosis
                     protein"
                     /codon_start=1
                     /product="BTB/POZ domain-containing protein 8 isoform 1"
                     /protein_id="NP_001365195.1"
                     /db_xref="GeneID:100503185"
                     /db_xref="MGI:MGI:3646208"
                     /translation="MAHCGAGGAAPVALLSSPGHGRKGLPRKGPGERRRLKAAVSEQL
                     SRDVLRLLREEIHTDTILSVSGSLFKVHKAVLLARAPGFHSHIIEHTSSDLTNELVPV
                     DGVEASEFKAFLQIVYSSNKNIKNYEEEIVKKLKVGSLMPEKGPDVSFPRYRTSSDCF
                     LGKGEIPEDITGGGDCFISKADSDLEPASELGGDLLKLYLSQHYPDIDICVDGRSFRA
                     HRAILSARSSYFAAMLSGCWAESSQECITLQGITHVEMNVMMHFIYGGTLDFPEKANV
                     GQILNVADMYGLEGLREVAIYVLRRDYCNFFQKPVPRTWASILECLIIAHSVGVESLF
                     ADCMNCIISHFARFWSERSFANVPPEIQKTCLSMQIQSLNHGNAAFLLMESDRLIMGL
                     PRVKWTEAALSMASQLQEECVTFIVENFSQIIESENFTLLLQSQAMSSTAHLLDKIFK
                     AIEDNITAENSCSLLMALDTLLNSESTKEMGFTCRIQAVRGKLWAFLVQSFYAVRHTG
                     SWKLMHLDDQQKIQVAALDKGDDRRLGRKPVLTSSQQRRQGSDVDVLKIKPWTENNKK
                     PCQTSLSTNQKMRSDGLGASGHASSTNRNSINKVSKHGDSTKMSKVVKEMKTGGKYVS
                     GKSKTMVKPQTENSDHTKIEGLSSTVVGRPSRVTAAGRKDPVHGKGVQNQEVETTGAR
                     PKVLTANLNVQARAKPLQTLRGKDSTCPASVGPSSRSTHSSTELLASMGSVDETKENG
                     SVEDKCRDGKPYVSDSPGQMVSNGVMSTAAVKSRAVARITNGTASNKSFTHEQDSHGN
                     SSVIKRGDGKGLSASAPQTAAKKRGSSNGCTAAQPRTKSAPPTLAQAQGSQGESPHSV
                     KSSVSSRQSDENVTRLRHGTDKQIPKRKVVKQGHTTLQKVNAKLVPMPKIPSQPKKGG
                     TVNSKDSKQKVLPGQIMLQSHASQRPSKPEVAEKSVLHGVSDSRNHVSKQRPHESPCT
                     LACDTSGPEVSQIPCRPQPQSPLGSQEKKRLELACQDRSTLGDSVKRELRSELAGIEQ
                     SHTSAYKDSSQCNGNPNCGSIAALKSVISNPNENLINSNPVHNSDSANTEQAYLSDRE
                     RETGRENTDTEPSMSCINGKAFLCVPEGTSSTLNPTQSDRRLTFHGAAQSQLPDDCAT
                     EDSKCATVTSAVSSKCLLGQPSEKNCKNMEMSETPESHGTPEAPFVGPWDLNTGATHQ
                     RESPESDTGSATTSSDDIKPRSEDYDAGGSQDDEGSHDRGISKCSTALCHDFLGRSSS
                     DTSTPEELKVHEGDLRTEVRVRKQGGGDHQIHSASDDEIPRKKPEPWSRSTIGYPREK
                     GSTPRGSIPSAQEGDQVSSSADETEDERSEAENVGENSSPCSSGTQQVQGIINLAFDD
                     GAEHESREFSATKKFRRSVLLSVDECEEVGSDEGEGHTPFQPSLDSLSPSDVFDGVSY
                     EHHGRTGYSRFFKENEATTAEHNHNKGNGGYKNESFLLNSRSKDFEKQDKQCVSTDQK
                     TRLDVPPKGSQQLFPENEEVISKREAAHSFQQHSTFMDGDTKSQERPCHLELHQRELS
                     SNIPKISSVKSLDSCPSQGLPQEGQVKESRPTPPKGANNAVFSGDIDDCDTMAQTSMY
                     DHRPSKTLSPIYEMSLTAACEQEVESETHVADRGSEDEQHFAKQDWTLLRQLLSEQDA
                     NLNITSSLPEDLSLAQYLINQTLLLARDSSKPQGSAHADTWNRWSELSSPLDDSATSA
                     TTASVSSTDCSPQGEWTILELETQH"
     exon            217..411
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            412..608
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            609..726
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            727..816
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            817..897
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            898..994
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1083
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1186
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1369
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1507
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1637
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1699
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..2375
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2466
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..2606
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            2607..4853
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     exon            4854..7062
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5412..5417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /note="hexamer: AATACA"
     polyA_site      5434
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /note="major polyA site"
     regulatory      5685..5690
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /note="hexamer: AATAAA"
     polyA_site      5714
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
     regulatory      7033..7038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
                     /note="hexamer: ATTAAA"
     polyA_site      7062
                     /gene="Btbd8"
                     /gene_synonym="A830010M20Rik; AI849226; EG627196; Gm16115;
                     mKIAA1107"
ORIGIN      
        1 gctgttgggc tcgggaacgc ccccgtaccg gtccagcgcg gcctggcacc ggggttctgc
       61 ggccgacatg gcccactgtg gagcgggagg tgcggccccg gtggcccttc tgagttcccc
      121 ggggcatggc aggaaggggc tgcccaggaa ggggcccggg gagcggcgcc ggctgaaggc
      181 ggcggtgtcg gagcagctca gccgcgacgt gctcaggctt ttaagggagg aaatccacac
      241 agacaccatt ctttctgtca gtggctcgct gttcaaagtg cacaaggcag tcttgttagc
      301 cagagctcct ggctttcatt cccatatcat tgaacacaca tcaagtgacc taacaaatga
      361 gcttgttcct gtggatggtg ttgaagcttc agaatttaaa gcatttttac agattgtata
      421 ttcatcaaac aaaaacataa aaaactatga agaggaaatt gttaaaaaac tgaaagtggg
      481 aagcttaatg ccagaaaagg ggccagacgt cagcttcccg cggtatagaa cttcgtctga
      541 ctgttttctt gggaaaggtg aaattccaga ggatattact ggtggaggtg actgctttat
      601 ttccaaggct gattctgacc tggagcctgc gtctgagtta ggaggagatt tgttgaagct
      661 ttacctgagt cagcactacc ctgacattga catttgtgtt gatgggagga gcttccgagc
      721 ccacagggcc attttaagtg ccaggtcgag ttattttgct gcaatgctga gtggctgctg
      781 ggctgaaagc tcccaagagt gtattactct tcaaggaata acccatgtag aaatgaatgt
      841 tatgatgcac tttatatatg gaggaacttt ggattttccc gaaaaggcta atgttggtca
      901 gatactgaac gtggctgata tgtatggact ggaaggattg agagaagtag caatctatgt
      961 tttaagaaga gattactgta atttctttca gaagcctgtt cccagaacgt gggcatcaat
     1021 tctagaatgc ttaatcattg ctcattcagt cggagtggaa agcctttttg ctgactgcat
     1081 gaactgcatt ataagccatt ttgcaaggtt ttggtcggaa agaagctttg caaatgttcc
     1141 tcctgagatt cagaaaacct gtctcagtat gcagattcag tccctaaatc atgggaatgc
     1201 tgcttttctt ctaatggaaa gtgacaggct gatcatgggt ttacccagag taaagtggac
     1261 agaagcagcc ctgtcgatgg catcccagct ccaagaagag tgtgtgacat ttattgttga
     1321 aaacttctct cagatcattg agagtgagaa cttcactctt ctcttgcagt cacaagcaat
     1381 gagcagcaca gctcatctgt tggacaaaat ttttaaagca attgaagaca atattaccgc
     1441 tgaaaatagt tgctctctcc ttatggctct ggacacgtta ctgaattctg aaagcacaaa
     1501 ggaaatgggc tttacgtgca ggatccaagc tgtgcgtggt aagctgtggg ccttcctggt
     1561 acagtctttc tatgctgttc gtcacacagg aagctggaaa ctgatgcact tagatgatca
     1621 gcagaaaatt caagtagctg cacttgacaa aggtgatgac cgcagacttg gcaggaagcc
     1681 tgtgctcacc agctctcagc aaaggagaca aggctctgat gttgatgttt taaaaataaa
     1741 accttggaca gaaaataaca aaaaaccgtg tcagacctct ctctctacta atcagaagat
     1801 gagatccgat ggattgggag catcaggaca tgcatcaagc actaacagaa acagtataaa
     1861 taaagtgtcc aagcatggtg acagtacaaa aatgtccaaa gttgtgaaag agatgaaaac
     1921 tgggggaaaa tatgtctctg gcaagtctaa aactatggta aagccccaaa cagaaaacag
     1981 tgatcataca aagatagaag gtttgtcatc cactgttgtg ggaagaccat caagagtgac
     2041 agcagctgga cggaaggacc cagtacatgg aaaaggagtc cagaaccaag aagtggagac
     2101 cacaggggca agacctaagg tactcactgc aaacctgaac gtgcaagcca gagccaagcc
     2161 tttgcaaaca ctgagaggaa aagattccac atgccccgcc tctgtagggc cctccagtag
     2221 atctacacat tccagcacgg agcttctggc ttccatgggc tctgtggatg agacaaagga
     2281 aaacggatca gtagaagaca agtgtcgtga tgggaagcca tacgttagtg attccccagg
     2341 acagatggtg agcaatggag taatgtcaac tgcagcagta aagtctcgag cagttgcaag
     2401 aattaccaat ggaacagcca gtaacaaaag ctttactcat gaacaagact ctcacgggaa
     2461 tagcagtgta ataaaaagag gggatggcaa aggacttagt gcttcagcac cacagacagc
     2521 tgcgaagaaa agagggagca gcaatggatg cacagcagct cagccacgga cgaagagcgc
     2581 ccctcccacc ctggctcaag ctcaaggatc ccaaggggag tcaccacact ctgtaaaatc
     2641 ttcagtctct tcaaggcagt ctgatgaaaa tgtaacaagg ttgaggcatg gaacagataa
     2701 gcaaatacct aagagaaaag ttgtcaagca aggacacaca actttgcaga aggttaatgc
     2761 aaagctggta ccaatgccta aaatcccaag tcagcccaag aaaggtggaa ctgtaaacag
     2821 caaagattca aaacaaaaag tgcttcctgg acaaattatg ctgcaaagtc atgcttctca
     2881 gcgaccttcc aaacctgagg tagctgagaa aagcgtgctt catggtgtga gtgacagcag
     2941 gaaccatgtc tctaaacaga ggcctcacga gtccccgtgt acccttgctt gtgacaccag
     3001 tggtcctgaa gtgtcccaga taccttgcag acctcagcca caaagtccat taggcagtca
     3061 agagaaaaag agactggagc tagcatgtca agataggtca accctgggcg actcagtgaa
     3121 acgtgagctg aggtcagaac tggctggcat agagcaaagt cacacatccg cttacaaaga
     3181 ctcgagtcag tgcaatggga atccaaactg tgggagcatt gctgctctga aatctgtgat
     3241 ttcaaatcca aatgaaaact tgataaactc taacccagtt cataattcag actcagcaaa
     3301 tacagagcaa gcctatttat cagatagaga gagagaaaca gggagagaaa acacagatac
     3361 tgaaccaagt atgagttgta taaatgggaa agctttcctg tgtgttcctg aagggacaag
     3421 cagtaccctc aaccctactc aaagtgacag aagactcaca tttcatggag cagctcaaag
     3481 ccagttacct gacgactgtg ctacagagga cagtaaatgt gctacagtca cctcagctgt
     3541 ttcttccaaa tgtcttttgg gacaaccatc agaaaaaaat tgtaaaaata tggaaatgtc
     3601 agaaacccca gagagccatg gaactccaga agctccattc gtgggcccct gggatttgaa
     3661 cactggtgcc acacatcaaa gagagagtcc agaatccgac actggtagtg ctaccacatc
     3721 ttcagacgac ataaagcccc gctcagaaga ctatgatgct ggggggtctc aggatgatga
     3781 gggctcacac gacagaggca tctccaagtg cagcacagcg ctgtgccatg acttccttgg
     3841 gaggagcagc agcgacacca gcacccctga agagttgaag gtgcacgagg gtgacctcag
     3901 gactgaagtc agagtgagaa agcagggtgg gggtgaccac cagatccact cggcaagtga
     3961 tgacgagatc cctaggaaga agccagaacc ttggtctcga tctacaatag gctatccccg
     4021 ggagaaggga agtacgccac ggggtagcat cccatctgct caggaagggg accaggtttc
     4081 ttcttcagca gatgaaacag aagatgaaag atctgaagca gagaatgttg gggaaaattc
     4141 ctctccatgc agctcaggta ctcagcaggt tcagggaata attaacttag cttttgacga
     4201 tggagctgaa catgaaagtc gtgagttttc tgcaactaag aagtttagaa ggtcagtgtt
     4261 gctctcggta gatgagtgtg aagaagtggg atctgatgaa ggtgaaggcc acaccccctt
     4321 tcagccttcc ctagattctc tttcaccttc tgatgttttt gatggtgttt cttatgagca
     4381 tcatggaagg acaggttact ctagattttt caaagaaaat gaagctacta ctgcagagca
     4441 taatcacaac aaaggcaatg gtggatataa aaatgaaagc tttctcctga attctagaag
     4501 caaagacttc gaaaaacaag ataaacaatg tgtctcaaca gatcaaaaga ccaggttaga
     4561 tgtcccaccc aaaggaagcc aacagctttt tccggaaaac gaagaagtga taagtaaacg
     4621 ggaggcggct cacagctttc agcagcacag cacattcatg gatggggaca ctaagtctca
     4681 agaacgaccg tgtcatttgg agctccatca gagagaactc agctccaata taccaaagat
     4741 cagctcagtg aaatctctgg actcctgtcc cagccagggg ctgcctcagg aagggcaagt
     4801 gaaagagagc cgtcccacgc ctcccaaggg agctaataac gctgtgttct caggagacat
     4861 agatgattgt gatacaatgg cacaaacctc catgtatgac caccggcctt caaaaaccct
     4921 ctctccaata tatgagatgt ctctaacagc agcgtgtgag caggaagtgg agtcagaaac
     4981 ccatgtcgca gacaggggct ctgaagacga gcagcacttt gcaaagcagg actggacact
     5041 gctgaggcag ctgctttctg aacaagacgc aaacttaaat atcacaagct ctctgcctga
     5101 agacctaagc ttagcccagt atttaatcaa ccagacactc ctcttagctc gagatagctc
     5161 gaaacctcag ggttcagcac acgctgacac ttggaacagg tggagtgaac tgtcatcccc
     5221 acttgatgat tcggcaacaa gtgccaccac ggctagtgtt tcttccacag attgttcgcc
     5281 tcaaggggag tggacaatcc tggaattgga aactcagcat taggagtctc agcagtcggg
     5341 aaagatttag actacgccac ccctgtattt ttgatgccta ctttatattt aaattataat
     5401 ttagttagcc aaatacagac tgtccttaaa ttgatggagt cttttaattt attgaggtaa
     5461 atatagctta tttattgata tgatgtctca tattttgaaa atgtttttct aaatttatga
     5521 gcatgttttc tacatcaatt aagttagaag gctgaaaaca gtctttgcta cagttttctt
     5581 tcctacctaa taatttgttc atttattcaa gaatttccat gtcagtacac aagtagatca
     5641 accccttact cttgactctg catatggtat cataataatt taacaataaa cttactgtgt
     5701 gagtgtgcac cgtacagaat cagtctgtga ataacattca gactcaggtg agtgttgcgt
     5761 ctggggacac agagggaccc ccagcttccc cagtgtttca ggactaggac atggagaggt
     5821 gagtgcacct tgagtttcag ccatactgct aataaactaa gaggagttgg gcttttctgt
     5881 ttatgtcagg gataggaatt tatttcctct cttcaaaggc atgtagaaac tgttttgttt
     5941 gtggctggct ctaacagtca ctttgtcttg catgccatgg taggaatact ttggagctgc
     6001 actgctctga gctaacacag cattaaccaa aattcgtgca tgaggcagct gagtgagtcc
     6061 tagtgtgggc gtgtgcttcc tcttgtctgt ttatgtcctt tgtctgtcag atttctctca
     6121 gtctgtagct ttgtaaaaaa ccagataatc agtgctacaa gagaggagca gtaggatctt
     6181 agatgctaat gaggatccct ttaaggtgat gggaaaataa caaccaggtt aatacagaac
     6241 tatttgtccc aaaacaacaa ttacgttttc atttaagcac tcatctatta taccataaaa
     6301 gtttctcatg aattttaaaa aaggttttaa gtgttttata ttaatttatt ttgtgggtag
     6361 gcatgtgcat gtgtgctaca acacacacgt ggaagtcagt ggacagcctg tgagtcagct
     6421 ctctccttcc accacgggag tcccagggac tgaagtcagg tcatcaggct tggtggcgag
     6481 tgcctttacc cactgagcca tccctccgat cctggttcta tgccttttta aaaagctaga
     6541 cactcttgca ggactactca ttcatgtggg gcttagttat actcagctca acaggtaaat
     6601 aaataggcat tggatgtaag cactcaatag agggtaaaat agaattaagt aaaaagttcg
     6661 gtttctagtg tagggcagga gaatgtaagg aaaacattga aatatgaaca cacacacaca
     6721 cacatacttt atatcttaaa gcttggtaga aatttaaaga ttagactagt ctctttaaat
     6781 ggtaaaggaa ggcttttgtg gccataagaa tgaaaaggtc ctacagccta ctcgtgaggt
     6841 agaatgcatt atttcttaat tccaacttgc tgttatttct accacacaga acactctttc
     6901 tcatggagct gtgaacaaac aatgacacac tgaaacattt gtagtgtgaa aatgtctatg
     6961 tgatgacaaa gtgcaccttc aaacattgac acactgaaac atttgtagta tggaaatgac
     7021 tatgtgatga caattaaagt gcaccttcat tcattcccaa ga
//
