LOCUS       NM_001136064            1962 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Mus musculus BSCL2 lipid droplet biogenesis associated, seipin
            (Bscl2), transcript variant 1, mRNA.
ACCESSION   NM_001136064
VERSION     NM_001136064.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   McClure,J.J., McIlroy,G.D., Symons,R.A., Clark,S.M., Cunningham,I.,
            Han,W., Kania,K., Colella,F., Rochford,J.J., De Bari,C. and
            Roelofs,A.J.
  TITLE     Disentangling the detrimental effects of local from systemic
            adipose tissue dysfunction on articular cartilage in the knee
  JOURNAL   Osteoarthritis Cartilage 32 (12), 1552-1565 (2024)
   PUBMED   39103079
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Guo,D., Hu,L., Xie,P., Sun,P. and Yu,W.
  TITLE     Seipin is involved in oxygen-glucose deprivation/reoxygenation
            induced neuroinflammation by regulating the TLR3/TRAF3/NF-kappaB
            pathway
  JOURNAL   Int Immunopharmacol 134, 112182 (2024)
   PUBMED   38703568
  REMARK    GeneRIF: Seipin is involved in oxygen-glucose
            deprivation/reoxygenation induced neuroinflammation by regulating
            the TLR3/TRAF3/NF-kappaB pathway.
REFERENCE   3  (bases 1 to 1962)
  AUTHORS   Li,Z., Jin,S., Xu,T., Chen,H., Cai,W., Du,J., Qiu,J., Zhuang,S.,
            Qi,Y., Gu,W. and Pang,L.
  TITLE     Effects of Seipin on Mouse Mesenchymal Stem Cell Osteo-Adipogenic
            Balance
  JOURNAL   Stem Cells Dev 33 (7-8), 177-188 (2024)
   PUBMED   38386508
  REMARK    GeneRIF: Effects of Seipin on Mouse Mesenchymal Stem Cell
            Osteo-Adipogenic Balance.
REFERENCE   4  (bases 1 to 1962)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 1962)
  AUTHORS   Shen,W.J., Cortez,Y., Singh,A., Chen,W., Azhar,S. and Kraemer,F.B.
  TITLE     Mice deficient in ER protein seipin have reduced adrenal
            cholesteryl ester lipid droplet formation and utilization
  JOURNAL   J Lipid Res 63 (12), 100309 (2022)
   PUBMED   36332685
  REMARK    GeneRIF: Mice deficient in ER protein seipin have reduced adrenal
            cholesteryl ester lipid droplet formation and utilization.
REFERENCE   6  (bases 1 to 1962)
  AUTHORS   Schwindinger,W.F., Giger,K.E., Betz,K.S., Stauffer,A.M.,
            Sunderlin,E.M., Sim-Selley,L.J., Selley,D.E., Bronson,S.K. and
            Robishaw,J.D.
  TITLE     Mice with deficiency of G protein gamma3 are lean and have seizures
  JOURNAL   Mol Cell Biol 24 (17), 7758-7768 (2004)
   PUBMED   15314181
REFERENCE   7  (bases 1 to 1962)
  AUTHORS   Floyd,J.A., Gold,D.A., Concepcion,D., Poon,T.H., Wang,X.,
            Keithley,E., Chen,D., Ward,E.J., Chinn,S.B., Friedman,R.A.,
            Yu,H.T., Moriwaki,K., Shiroishi,T. and Hamilton,B.A.
  TITLE     A natural allele of Nxf1 suppresses retrovirus insertional
            mutations
  JOURNAL   Nat Genet 35 (3), 221-228 (2003)
   PUBMED   14517553
REFERENCE   8  (bases 1 to 1962)
  AUTHORS   Kaisaki,P.J., Sebag-Montefiore,L.M., Brown,J.H., Magre,J.,
            Lathrop,M., Capeau,J. and Gauguier,D.
  TITLE     Localization, cDNA sequence and genomic organization of the rat
            seipin gene (Bscl2) and sequence analysis in inbred rat models of
            Type 2 diabetes mellitus
  JOURNAL   Cytogenet Genome Res 98 (1), 71-74 (2002)
   PUBMED   12584444
REFERENCE   9  (bases 1 to 1962)
  AUTHORS   Inoue,S., Sano,H. and Ohta,M.
  TITLE     Growth suppression of Escherichia coli by induction of expression
            of mammalian genes with transmembrane or ATPase domains
  JOURNAL   Biochem Biophys Res Commun 268 (2), 553-561 (2000)
   PUBMED   10679242
REFERENCE   10 (bases 1 to 1962)
  AUTHORS   Downes,G.B., Copeland,N.G., Jenkins,N.A. and Gautam,N.
  TITLE     Structure and mapping of the G protein gamma3 subunit gene and a
            divergently transcribed novel gene, gng3lg
  JOURNAL   Genomics 53 (2), 220-230 (1998)
   PUBMED   9790771
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK618551.1, BC061689.1 and AC129217.3.
            
            On Mar 20, 2014 this sequence version replaced NM_001136064.2.
            
            Transcript Variant: This variant (1) is the longer transcript and
            encodes the longer isoform (1). Variants 1 and 2 encode the same
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061689.1, SRR7652917.975460.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-262               CK618551.1         1-262
            263-1946            BC061689.1         1-1684
            1947-1962           AC129217.3         81467-81482
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 5.76 cM"
     gene            1..1962
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /note="BSCL2 lipid droplet biogenesis associated, seipin"
                     /db_xref="GeneID:14705"
                     /db_xref="MGI:MGI:1298392"
     exon            1..398
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            399..607
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     CDS             533..1864
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /note="isoform 1 is encoded by transcript variant 1;
                     seipin; bernardinelli-Seip congenital lipodystrophy type 2
                     protein homolog; G protein gamma 3 linked;
                     Bernardinelli-Seip congenital lipodystrophy 2 homolog;
                     Berardinelli-Seip congenital lipodystrophy 2 homolog
                     (seipin); Berardinelli-Seip congenital lipodystrophy 2
                     (seipin)"
                     /codon_start=1
                     /product="seipin isoform 1"
                     /protein_id="NP_001129536.1"
                     /db_xref="CCDS:CCDS29551.2"
                     /db_xref="GeneID:14705"
                     /db_xref="MGI:MGI:1298392"
                     /translation="MIHQRREAGARETCRDQIKGSDKDEEPSAALSHGQGYRPCGRPA
                     RNSKPEAGARPPAVPIMVNDPPVPALLWAQEVGHVLAGRARRLMLQFGVLFCTILLLL
                     WVSVFLYGSFYYSYMPTVSHLSPVHFHYRTDCDSSTASLCSFPVANVSLAKSGRDRVL
                     MYGQPYRVTLELELPESPVNQDLGMFLVTVSCYTRGGRIISTSSRSVMLHYRSQLLQV
                     LDTLLFSSLLLFGFAEQKQLLEVELYSDYRENSYVPTTGAIIEIHSKRIQMYGAYLRI
                     HAHFTGLRYLLYNFPMTCAFVGVASNFTFLSVIVLFSYMQWVWGAVWPRHRFSLQVNI
                     RQRDNSHHGAPRRISRHQPGQESTQQSDVTEDGESPEDPSGTEGQLSEEEKPEKRPLN
                     GEEEQEPEASDGSWEDAALLTEANPPTSASASALAPETLGSLRQRPTCSSS"
     exon            608..924
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1006
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1150
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1285
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1383
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1525
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1592
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1661
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1742
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1962
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1928..1933
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /note="hexamer: AATAAA"
     polyA_site      1946
                     /gene="Bscl2"
                     /gene_synonym="2900097C17Rik; Gng3lg"
                     /note="major polyA site"
ORIGIN      
        1 cagccgccat cttgggcatt ggctgccgcg ccgagtcgcc ctgtcccgcc ccacggcctc
       61 tcgggccacc tccccccggg gctgctgtcc ctgggcctgc ggaaacccac cctgctcgcc
      121 cttgtgtggt tctcgccgct tacgttgcgc gctcgtgtga atgctccgcg gcttctgagc
      181 gctcgtttta acaacccccc actgggggcg ctcgggcacc ttatactttg cgatctccaa
      241 tccttacaat gtctaacggg ctcgactggc cctgttccct ggatatggca agtgtatcga
      301 ttaaggatcc tctttcccaa gaacgctaac acagacgttg tcctctgagg ctctgaatga
      361 ctttattctt caaatgcctt tacctttcat agctccagtg acccaccgtt cttcagagct
      421 aggaaaatgt aacactctac aggtgatcag cttctagatc accactttct accacgtgag
      481 tgacgcgagc tgggagaccc agaggtaacc catcaggaag atagctaaag aaatgataca
      541 tcaaagaaga gaagctgggg caagagagac atgcagagac cagatcaaag gatcagacaa
      601 agacgaggaa ccttcagctg ccctatccca tggccaaggg tatcgtccct gtggaagacc
      661 agccaggaac tcaaagcctg aggctggggc cagaccccct gctgtcccca tcatggtcaa
      721 tgacccacca gtccccgcct tactgtgggc ccaggaagtg ggccacgtct tggcaggccg
      781 tgcccgcagg ctgatgctgc agtttggggt gctcttctgc accatccttc ttttgctttg
      841 ggtgtctgtg ttcctctatg gctccttcta ctactcctac atgccgacgg tcagccacct
      901 cagccctgtg cacttccact acaggacaga ctgtgattcc tccaccgcct ctctctgctc
      961 tttccctgtt gccaatgtct cactggctaa gagtggacgt gatcgggtac tgatgtatgg
     1021 acagccatat cgagtcacct tagagcttga actgcccgaa tcccctgtga atcaagactt
     1081 gggcatgttc ttagtcactg tttcatgtta taccagaggt ggccgaatca tctccacttc
     1141 ttcacgctca gtgatgctgc attaccgctc gcagctgctc caggtgctcg acacgctgct
     1201 gttctccagc ctcctgctgt ttggcttcgc tgaacagaag cagctcctgg aagtagaact
     1261 ctactctgac tatagggaga attcgtatgt gccaacaact ggagcaatta ttgagatcca
     1321 cagcaagcgc atccagatgt atggagccta cctccggatc catgcccact tcactgggct
     1381 caggtacctg ctgtacaact tccccatgac ctgcgccttc gtgggtgtgg ccagcaactt
     1441 cacgttcctc agcgtcatcg tgctcttcag ctatatgcag tgggtgtggg gtgctgtctg
     1501 gccccgccac cgcttctctc tgcaggttaa tatccgacaa agggataact cacaccacgg
     1561 ggccccgcgt cggatctcgc gccatcagcc aggtcaggaa tctacccagc agtcggatgt
     1621 gacggaggat ggtgagagcc ctgaagatcc ctcaggaaca gagggccagc tgtctgagga
     1681 agagaaacca gagaagcggc ccctgaatgg agaggaggag caagagccag aggctagtga
     1741 tggctcctgg gaagatgcag ctttgctgac agaggccaac ccacctacct ctgcctcagc
     1801 atctgccctt gcccctgaga ctctgggtag cctcaggcaa cgcccgacct gctccagttc
     1861 ctgaacaaag ggtgaattcc tcacattcca gcgctttccc atcatacacc ttttcccttg
     1921 ctcccttaat aaactatttt gtggcagctg cttgtgtgga ca
//
