LOCUS       NM_001009930            4507 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript
            variant 3, mRNA.
ACCESSION   NM_001009930
VERSION     NM_001009930.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Xu,R., Wang,K., Yao,Z., Zhang,Y., Jin,L., Pang,J., Zhou,Y.,
            Wang,K., Liu,D., Zhang,Y., Sun,P., Wang,F., Chang,X., Liu,T.,
            Wang,S., Zhang,Y., Lin,S., Hu,C., Zhu,Y. and Han,X.
  TITLE     BRSK2 in pancreatic beta cells promotes hyperinsulinemia-coupled
            insulin resistance and its genetic variants are associated with
            human type 2 diabetes
  JOURNAL   J Mol Cell Biol 15 (5) (2023)
   PUBMED   37188647
  REMARK    GeneRIF: BRSK2 in pancreatic beta cells promotes
            hyperinsulinemia-coupled insulin resistance and its genetic
            variants are associated with human type 2 diabetes.
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Nakanishi,K., Niida,H., Tabata,H., Ito,T., Hori,Y., Hattori,M.,
            Johmura,Y., Yamada,C., Ueda,T., Takeuchi,K., Yamada,K.,
            Nagata,K.I., Wakamatsu,N., Kishi,M., Pan,Y.A., Ugawa,S.,
            Shimada,S., Sanes,J.R., Higashi,Y. and Nakanishi,M.
  TITLE     Isozyme-Specific Role of SAD-A in Neuronal Migration During
            Development of Cerebral Cortex
  JOURNAL   Cereb Cortex 29 (9), 3738-3751 (2019)
   PUBMED   30307479
  REMARK    GeneRIF: Isozyme-Specific Role of SAD-A in Neuronal Migration
            During Development of Cerebral Cortex.
REFERENCE   3  (bases 1 to 4507)
  AUTHORS   Chu,Q., Yao,C., Qi,X., Stripp,B.R. and Tang,N.
  TITLE     STK11 is required for the normal program of ciliated cell
            differentiation in airways
  JOURNAL   Cell Discov 5, 36 (2019)
   PUBMED   31636950
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4507)
  AUTHORS   Wang,K., Liu,D., Zhang,Y., Chang,X., Xu,R., Pang,J., Li,K., Sun,P.,
            Zhu,Y. and Han,X.
  TITLE     SAD-A, a downstream mediator of GLP-1 signaling, promotes the
            phosphorylation of Bad S155 to regulate in vitro beta-cell
            functions
  JOURNAL   Biochem Biophys Res Commun 509 (1), 76-81 (2019)
   PUBMED   30573363
  REMARK    GeneRIF: SAD-A is activated by the incretin hormone GLP-1 in
            pancreatic beta-cell. SAD-A promotes the phosphorylation of Bad
            S155.
REFERENCE   5  (bases 1 to 4507)
  AUTHORS   Nie,J., Sun,C., Chang,Z., Musi,N. and Shi,Y.
  TITLE     SAD-A Promotes Glucose-Stimulated Insulin Secretion Through
            Phosphorylation and Inhibition of GDIalpha in Male Islet beta Cells
  JOURNAL   Endocrinology 159 (8), 3036-3047 (2018)
   PUBMED   29873699
  REMARK    GeneRIF: Glucose and incretin stimulate insulin secretion through
            the phosphorylation of GDIalpha at Ser174 by SAD-A, which leads to
            the activation of Rho GTPases, culminating in insulin exocytosis.
REFERENCE   6  (bases 1 to 4507)
  AUTHORS   Barnes,A.P., Lilley,B.N., Pan,Y.A., Plummer,L.J., Powell,A.W.,
            Raines,A.N., Sanes,J.R. and Polleux,F.
  TITLE     LKB1 and SAD kinases define a pathway required for the polarization
            of cortical neurons
  JOURNAL   Cell 129 (3), 549-563 (2007)
   PUBMED   17482548
  REMARK    GeneRIF: Once activated, LKB1 phosphorylates and thereby activates
            SAD-A and SAD-B kinases, which are also required for neuronal
            polarization in the cerebral cortex.
REFERENCE   7  (bases 1 to 4507)
  AUTHORS   Munton,R.P., Tweedie-Cullen,R., Livingstone-Zatchej,M.,
            Weinandy,F., Waidelich,M., Longo,D., Gehrig,P., Potthast,F.,
            Rutishauser,D., Gerrits,B., Panse,C., Schlapbach,R. and Mansuy,I.M.
  TITLE     Qualitative and quantitative analyses of protein phosphorylation in
            naive and stimulated mouse synaptosomal preparations
  JOURNAL   Mol Cell Proteomics 6 (2), 283-293 (2007)
   PUBMED   17114649
REFERENCE   8  (bases 1 to 4507)
  AUTHORS   Kishi,M., Pan,Y.A., Crump,J.G. and Sanes,J.R.
  TITLE     Mammalian SAD kinases are required for neuronal polarization
  JOURNAL   Science 307 (5711), 929-932 (2005)
   PUBMED   15705853
  REMARK    GeneRIF: results show that SAD-A and SAD-B, mammalian orthologs of
            a kinase needed for presynaptic differentiation in Caenorhabditis
            elegans, are required for neuronal polarization.
REFERENCE   9  (bases 1 to 4507)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   10 (bases 1 to 4507)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772165.15, AK158574.1, AL603836.13 and BE986320.1.
            
            On Oct 13, 2012 this sequence version replaced NM_001009930.2.
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longest isoform (gamma).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY533674.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-256               AL772165.15        120844-121099
            257-610             AK158574.1         2-355
            611-1190            AK158574.1         360-939
            1191-1191           AL603836.13        189813-189813       c
            1192-2277           AK158574.1         941-2026
            2278-2409           BE986320.1         1-132               c
            2410-4507           AL603836.13        173360-175457       c
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 87.43 cM"
     gene            1..4507
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /note="BR serine/threonine kinase 2"
                     /db_xref="GeneID:75770"
                     /db_xref="MGI:MGI:1923020"
     exon            1..429
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     CDS             336..2495
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.26"
                     /note="isoform gamma is encoded by transcript variant 3;
                     BR serine/threonine-protein kinase 2;
                     serine/threonine-protein kinase SAD-A; brain-specific
                     serine/threonine-protein kinase 2;
                     serine/threonine-protein kinase BRSK2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase BRSK2 isoform
                     gamma"
                     /protein_id="NP_001009930.1"
                     /db_xref="CCDS:CCDS22022.1"
                     /db_xref="GeneID:75770"
                     /db_xref="MGI:MGI:1923020"
                     /translation="MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQ
                     KVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGG
                     ELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDERNNIRIAD
                     FGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFD
                     DDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKN
                     EPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMI
                     YFLLLDRKERYPSHEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDG
                     GSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPV
                     HTPKESPAGTPNPTPPSSPSVGGVPWRTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSN
                     LTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVI
                     SQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFK
                     RVVETIQAQLLSTHDQPSAQHLSDTTNCMEVMTGRLSKCDEKNGQAAQAPSTPAKRSA
                     HGPLGDSAAAGPGGDTEYPMGKDMAKMGPPAARREQP"
     exon            430..524
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            525..610
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            611..751
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            752..868
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            869..902
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            903..971
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1118
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1150
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1315
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1413
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1564
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1625
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1833
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1882
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..2006
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2187
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2277
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2325
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..4507
                     /gene="Brsk2"
                     /gene_synonym="4833424K13Rik; SAD-A; SADA"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctcggttc ggttgctcgg cggggctcgg ctgcacggcc gcggacgggc gtgcgctggg
       61 ggggggcgcg gggcgcgggc ctcggaggtg gcggcggaag cctggtgccc ccgcccgccg
      121 ggtcctctca gagaggcaga gcggcattcc cgcggcccgg cctcgtacgg gtgcggcgtg
      181 gggggcggcg ggggcgcgga gcagcgcggc ccgcccgggg gcgccgcggg ccgggtcagc
      241 gcggacggcg ctcggcggac tcgggcgcac tgctggccgg cccctccctg cccgcgcgcc
      301 cgggcgccca ctcgccggcg cggggcccgg gagcgatgac atcgacgggg aaggacggcg
      361 gcggggcgca gcacgcgcag tatgtggggc cctaccggct ggagaagacg ctgggcaagg
      421 ggcagacagg cttggtgaag ctgggaatcc actgtgtcac ttgccagaag gtcgccatca
      481 aaatcgtgaa ccgtgagaag ctcagtgagt cggtgctgat gaaggtggag cgagagattg
      541 ccatcttgaa gctcatcgag catccacatg tactgaagct gcatgacgtc tatgaaaaca
      601 aaaaatattt atacctggtg ctagaacatg tgtctggggg agagctgttc gactacctgg
      661 tgaagaaggg ccggctgacc cccaaggagg cccgcaagtt cttccggcag atcatctctg
      721 cactggactt ctgtcacagc cactccatat gccatagaga cttgaagcca gagaacctgc
      781 tgctagatga gaggaacaac atccgtattg cagactttgg catggcatcc ctgcaggtgg
      841 gagacagcct gctggagacc agctgcggat ctccacacta tgcctgtccg gaagtgattc
      901 ggggcgagaa gtatgatggc cgcaaggcag atgtgtggag ctgtggtgtg atcctgttcg
      961 ccttgctggt gggggctctg ccttttgatg atgacaacct gcggcagttg ctggagaagg
     1021 tcaagcgtgg tgtgttccac atgccacact ttatcccacc agactgccag agtctcctgc
     1081 gtggcatgat tgaggtggat gcagctcggc gcctcacgct agagcacatt cagaaacaca
     1141 tatggtatat aggtggcaag aatgagccag agcccgaaca gcccatccca cgcaaggtgc
     1201 agatccgctc actacccagc ttggaagaca ttgaccctga tgtgttggac agcatgcact
     1261 cactgggctg cttccgagac cgcaacaagc tgctgcagga tctgctatct gaggaggaga
     1321 atcaggaaaa gatgatttat ttcctcctcc tggatcggaa agaacggtat ccaagccatg
     1381 aggatgagga cctgcccccc aggaatgaga tagaccctcc ccggaagcgt gtggattccc
     1441 cgatgctgaa ccggcatggc aagcggcgac ctgagcgcaa gtccatggaa gtgctcagtg
     1501 tgacagatgg tggctcccca gtgcctgcac ggagagccat tgagatggcc cagcatggcc
     1561 agagatctcg atccatcagt ggtgcgtcct caggcctttc tacaagtcca ctcagcagtc
     1621 ctcgggtgac ccctcacccc tcaccaaggg gtagtcccct tcctaccccc aaagggacgc
     1681 ctgtccacac gccaaaggag agcccagctg gcacacccaa ccccacacca ccatccagcc
     1741 ctagtgttgg aggagtgccc tggcggacac gactgaactc catcaagaac agcttcctgg
     1801 gctcacctcg attccaccgc cggaaactcc aagttcccac gccagaggag atgtccaacc
     1861 tgaccccaga atcctctcca gagctggcca agaaatcgtg gttcgggaac ttcatcaacc
     1921 tggagaagga ggagcagatc tttgtggtga tcaaggacaa gcccctgagc tccatcaagg
     1981 ctgacatcgt tcatgccttc ctgtcgatcc ccagcctcag ccacagcgtt atttcccaga
     2041 caagcttcag ggctgaatac aaggccacag ggggcccagc agtgttccag aagccggtca
     2101 agttccaggt ggacatcacc tacactgagg gcggagaggc ccagaaggag aatggcatct
     2161 actcagtcac attcacttta ctctcaggcc ccagtcgccg cttcaagagg gtggtggaga
     2221 ccatccaggc ccagctgtta agcacccatg accagccatc agcccagcac ctgtcagaca
     2281 ccactaactg tatggaagtg atgacggggc ggctttccaa atgtgacgag aagaacgggc
     2341 aggcggccca ggcccccagc acacccgcca agcggagtgc ccacggcccc ctgggtgact
     2401 ccgcggccgc tggccctgga ggggacaccg agtacccgat gggcaaggac atggccaaga
     2461 tggggccgcc cgccgcccgc cgtgagcagc cttagacact agtccctccc cagcacagca
     2521 cagcacagca caacacagca ctgactgtgg ccttctgccg ccctgtgtgg acaaggcccc
     2581 acctgcctgc ccagaccttg tctcaagcct gggaggaaag tagagtggcc agggcgcggc
     2641 ccgcaggctg ggctgctggg cttgctctct tttctctctt gcggtctctg tctctgcctg
     2701 tctctgacag catcgcttgt ttccgctctg ataccaggaa ttatcccgaa aagttaacat
     2761 gtcacctcca cgaggccatc ctctgtgctg cgtgtggaca ctggctgacc gaaggcagct
     2821 gctgcggacc accctcctcc tgctgctgct gctgggcaga gaggctagcc cactatccac
     2881 cacctctgcc tgtcccctgg cagctccgca tggcccaagg gaggagggcc tgccgcccag
     2941 gggttcctct tctcgcccag ccaccctgga tcccagtgac cagatcctca gcagggacaa
     3001 cctgtccacc atcttccagg gtaggggacc atggtgggtg aagcagtggg aatggattgt
     3061 cctccacgcc gcatctggag gcctcctggg ccactcacct cgccttttcc acatgttggc
     3121 ctcgcagggc cacctcttct catctgcatc cattgctcca ccttgccagg ctgcagagct
     3181 ccagctgtga tggttctcat gggctgaagt ggagataaaa gacctgggtg ctccagagct
     3241 gccagggagc tgggctgaga ccagtattat ttatttgctg ttttaattta ttgagtttct
     3301 ttatgtctct gttctctgtt cagggaagga ggagtagcag cagcagcacc ctcctgccgt
     3361 ctgtctgtct gtttgttctg tctgtctgac ttgggcaggg tgggtccaga gcccaggttc
     3421 cctctgagga cagagccgct gtggggctgg ggacagtgga gagccagcca gtggatgcag
     3481 cactggcaag ctactgtaaa ctttaaaaga attcctgcaa gatattttta taaactttct
     3541 tttcttggtt gtttttggaa aagggtgtgg ggtggggccg ctggggcaga gctagagttt
     3601 gtgtttcggc tccttgctct tggctatttc tatacacata tacatatata tatatatata
     3661 tatttaaaga acgatgtggt tagctctgtc ccgggttgtt ttattactac ttagcagcag
     3721 ctccagttcc acacgagagc tcaggatcca gggcgtcagc ctgcactggt cagctgtgcc
     3781 tgctgcccag aagcactgcc cagtacttct ctgctgcatc aggctgaggc tgatcttggt
     3841 ttggtgtctg attgagaagg ggtagaaggc cctgagctgg caccctactc acacacagga
     3901 tggcagcagt acctcggttt gtttggtttt ttttttttta aattaaaatt aaaaagttta
     3961 aaaaaaaaat caaagccaga aatggagtta gcagagtgct ctgcagtggg ttcctcttgg
     4021 aggtggcgag ggcaaggggc ccacaccact gcctgctgcc caccaaagcc cacatcactt
     4081 ccctcatctt cagcttttgt ctgtgttccg ttctgtctgg ctgagccagt ccctcccagc
     4141 tggcccaagg tgcctggcat agcagcagga ggggctgggg gcaggtttca tgcatgcctt
     4201 tgctcttccc ctcatgtaca gattttaatg cttctattac attcaaccac cacaggctgg
     4261 gatttctata catagaacaa aatcaaacac ctgtaacaac agcaaagcca cgcagtacaa
     4321 gtgggaaggg gcatatctac ggtgtcagga tactgaaagc aatacctctc gttagccttc
     4381 ctcttttgta ctttggacac atgcggattc gcagacatca caaaaatgga caagccccat
     4441 ctggtgtaac ccctgtcaaa taactgtgag caacttcagt tctggaacaa taaattcctt
     4501 ccataaa
//
