LOCUS       NM_027861               3446 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus BRO1 domain and CAAX motif containing (Brox),
            transcript variant 1, mRNA.
ACCESSION   NM_027861 XM_135707
VERSION     NM_027861.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ichioka,F., Kobayashi,R., Katoh,K., Shibata,H. and Maki,M.
  TITLE     Brox, a novel farnesylated Bro1 domain-containing protein that
            associates with charged multivesicular body protein 4 (CHMP4)
  JOURNAL   FEBS J 275 (4), 682-692 (2008)
   PUBMED   18190528
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   3  (bases 1 to 3446)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   4  (bases 1 to 3446)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AEKQ02108040.1 and CAAA01119163.1.
            
            On Jan 26, 2019 this sequence version replaced NM_027861.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC030340.1, AK050525.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-106               AEKQ02108040.1     2473-2578
            107-194             CAAA01119163.1     28541-28628         c
            195-311             CAAA01119163.1     28098-28214         c
            312-418             CAAA01119163.1     26239-26345         c
            419-515             CAAA01119163.1     25671-25767         c
            516-611             CAAA01119163.1     21505-21600         c
            612-684             CAAA01119163.1     19890-19962         c
            685-790             CAAA01119163.1     18801-18906         c
            791-880             CAAA01119163.1     17897-17986         c
            881-966             CAAA01119163.1     16250-16335         c
            967-1048            CAAA01119163.1     14979-15060         c
            1049-1199           CAAA01119163.1     14564-14714         c
            1200-1359           CAAA01119163.1     13234-13393         c
            1360-3446           CAAA01119163.1     10116-12202         c
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1"
     gene            1..3446
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /note="BRO1 domain and CAAX motif containing"
                     /db_xref="GeneID:71678"
                     /db_xref="MGI:MGI:1918928"
     exon            1..106
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            107..194
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            195..311
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            312..418
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            419..515
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            516..611
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            612..684
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            685..790
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            791..880
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            881..966
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1048
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1199
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1359
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..3446
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2157..2162
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2179
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
     regulatory      3425..3430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3444
                     /gene="Brox"
                     /gene_synonym="0610010K06Rik"
                     /note="major polyA site"
ORIGIN      
        1 agttatctcg gtgctaccgg cgccttcctt gtctcgctgg aggactccgt cccaccttcc
       61 ctttccttca gcaggaagag tggccttacg gactctgagg tccccgccat ccaaccagag
      121 tctcagcttt gctttgcaga agtctaatct ggaagccctg cagtgtcagc acctcaggct
      181 tctcccacag actgagcaca ttttgtgaag atgacccatt ggtttcatag gaacccatta
      241 aaagctacag ctcccgtgtc ttttaactac tatggcatga tcactggtcc tcccgcttca
      301 aagatttgca atgacttgag gtccgccagg actcggctcc tcgagttgtt cactgatttg
      361 agctgtaatc cagaaacaat gaagaatgcg gcagacttgt atttctctct tttacaaggt
      421 ttcataaatt cagtgggtga ctctacacag gaaagcaaat tacgatacat tcagaatttc
      481 aagtggaccg atacattgca aggacacgtt ccgagtgccc agcaggatgc tgtttttgaa
      541 ctgatttcca tgggatttaa tgtagcgctg tggtacacaa aatatgcttc aagattggct
      601 ggaaaagaaa acataacaga agatgaagca aaagaagttc accgaagcct aaaaattgca
      661 gctgggattt ttaaacattt aaaggaaagt catatcccca aacttcttac accagcagaa
      721 aaggggcggg acttggaggc tcggctcatc gatgcttaca tcatccagtg ccaggccgag
      781 gctcaggaag tgacaatcgc ccgagccatt gagctgaagc acgctccggg actgatcgct
      841 gcactcgcct atgacacagc cagcttctac cagaaagcag atcatacttt gtccagtttg
      901 gagcctgcac actctgctaa atggagaaag taccttcact tgaagatgtg cttctacaca
      961 gcgtatgcgt attgctacca tggccagact ctgttggcga gcgataagtg tggagaagcc
     1021 atccgatctc tccaagaagc agaaaaattg tacgcagagg cagaagccct atgcaaagag
     1081 tacggggaaa ccaaaggacc agggccgacg gccaagccgt caggacactt gttcttccgg
     1141 aagcttggga gccttgtgaa gaacaccctg gacaaatgtc agagagaaaa tggcttcatt
     1201 tactttcaaa aaattccaac agaagcccca caactggaac tcaaagcaaa ttatgggctg
     1261 gtagagcctg tgccttttga attccctcct atgagtgctc actggacacc ggaagcgttg
     1321 gctgcgtttg atctcaccaa gagacccaag gatgacagtg tcaaacccaa accggaagag
     1381 gacgtgaaac ctgtaaagga accagatatc agacctcaga aggacactgg gtgctcggtc
     1441 tcataagaat gtctgtcact cactgcaatc tctgctggtc cacaagagaa actgtggggc
     1501 ttcagttcgc cgtttctgat ttctctgaag cccacaggat aattgttagc ttcagagcaa
     1561 cttggccttt catttattta ttcttgattt ttaacataga ggcatttcat gattttgctt
     1621 ctgggctctg cttttttaat caggacaaca tttggaaggg ttgtttttta tgcctctaat
     1681 gagtatatgt gggggattgg accttttctg aactctagat agtaagtttc atgttcatgt
     1741 ttttctactt tcacagaaat gtttgttctt cttgtttgat accagaagga tgttttgaaa
     1801 agagatgggt tcacctttca tcaccacatt gtcagatccg agtctagctt agtctaggtg
     1861 gggaaacaga atcgcagtta aaatcagaaa acctcagacc aagctctttc cctgtactag
     1921 gaaatcgtgt cttcaggaat atggaagtta cgtgaaattc agcagtctgg gtctgtgccc
     1981 ctcggcctgg ctcagcagcg gagggccgtg ctctctccac ccgcactacc cacacagtgg
     2041 tggttttctt acaagtacct tttctaatga actttttact taaggggtaa agctttgtac
     2101 tagcatttag tgcttctgtg tagagagagg aagagaatgg tagtgtgttt tgaagaatta
     2161 aaatgttgca tgttgtaaag aaagccttcg taaactcaac caagataggt tgtcctgact
     2221 cacactggta attcctttgt tagaagagtt ttaagaaaat gcctttgttc cctgattttg
     2281 gtggacactg tgtttttaac atggttttct tcagtatcct atagtaatgg cttggcgtta
     2341 ggccatagtg acagaggtta ggatatagac acacaaacta tgtgtttaaa ggatttgctt
     2401 atacccacac actgggaatg atgggtgtgt gatactttac caagtaacag atgtgggtgt
     2461 atcctgcttg gttgctctcc tggggtgtta acttcctttt ctaggtgaca gattgcttcg
     2521 cataatagga ataataatgt atgtgagaac ttactgtgtg ccagacagtg tataggcaca
     2581 tacgtagacc actgcatccc tttagccacc ctgtgagaaa cacatcaatc tgcataaagt
     2641 attgaaacag gaatatacat ctcatgatga agaatgaaga gattatacct gtcaatcaag
     2701 acagtagtca ataaatcata tatcatagtt tatactcctt tgaaaagcta catataatca
     2761 ctaagggttt tttaaaaaat aatttaaaat gtgagttaca gcttgcagta tctaatattt
     2821 aagctgtagc tttcagaata cttggagtaa ctactgaatg ggcattaagt tttattttgc
     2881 tgcctatagg aaaacaatac tttattacgt tgtcaccctt cctacagagt cacttgttaa
     2941 atgccagtga ctgacaaaac taactagtta ctgacaaaac taactagtac ttgctgctgc
     3001 ctgtttatac tctgcctgta ggacttgctc tgtgtactaa ccctcattaa cctaggtagg
     3061 cacgctgcgg tttctgtgga ctcgactgcc gttcatgaaa tggaaacaca tactatgtcc
     3121 ggcgcttctg taaaggcagt aaatgcaggt gttgaaatcc atcattggcc atgttcacgt
     3181 gatcgcatga gcagtcttgt tgattgtaaa gctaacacca gaagcctcgt atacgccggg
     3241 cgcaccatga ggttccgaag cagtagcagg gattgtgggg acgctgacta taaagcactg
     3301 gcaggcaggc tgtgcagaga tgacactgga cattggtgtt gcatgtgttt cataccaggc
     3361 gaggacagct cctgtacagc tacttctgta cagtgtttat atgatttatc tttttattta
     3421 tgaaaataaa agccatttta cttaca
//
