LOCUS       NM_054054               4710 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus bromodomain, testis-specific (Brdt), transcript
            variant 1, mRNA.
ACCESSION   NM_054054
VERSION     NM_054054.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   He,Z., Yan,R.G., Shang,Q.B. and Yang,Q.E.
  TITLE     Transcriptomic dynamics and cell-to-cell communication during the
            transition of prospermatogonia to spermatogonia revealed at
            single-cell resolution
  JOURNAL   BMC Genomics 26 (1), 58 (2025)
   PUBMED   39838296
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Wada,S., Ideno,H., Nakashima,K., Komatsu,K., Demura,N.,
            Tomonari,H., Kimura,H., Tachibana,M. and Nifuji,A.
  TITLE     The histone H3K9 methyltransferase G9a regulates tendon formation
            during development
  JOURNAL   Sci Rep 14 (1), 20771 (2024)
   PUBMED   39237663
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4710)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4710)
  AUTHORS   Dali,O., D'Cruz,S., Legoff,L., Diba Lahmidi,M., Heitz,C.,
            Merret,P.E., Kernanec,P.Y., Pakdel,F. and Smagulova,F.
  TITLE     Transgenerational epigenetic effects imposed by neonicotinoid
            thiacloprid exposure
  JOURNAL   Life Sci Alliance 7 (2), e202302237 (2023)
   PUBMED   37973188
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4710)
  AUTHORS   Her,Y.R., Wang,L., Chepelev,I., Manterola,M., Berkovits,B., Cui,K.,
            Zhao,K. and Wolgemuth,D.J.
  TITLE     Genome-wide chromatin occupancy of BRDT and gene expression
            analysis suggest transcriptional partners and specific epigenetic
            landscapes that regulate gene expression during spermatogenesis
  JOURNAL   Mol Reprod Dev 88 (2), 141-157 (2021)
   PUBMED   33469999
  REMARK    GeneRIF: Genome-wide chromatin occupancy of BRDT and gene
            expression analysis suggest transcriptional partners and specific
            epigenetic landscapes that regulate gene expression during
            spermatogenesis.
REFERENCE   6  (bases 1 to 4710)
  AUTHORS   Paillisson,A., Levasseur,A., Gouret,P., Callebaut,I., Bontoux,M.,
            Pontarotti,P. and Monget,P.
  TITLE     Bromodomain testis-specific protein is expressed in mouse oocyte
            and evolves faster than its ubiquitously expressed paralogs BRD2,
            -3, and -4
  JOURNAL   Genomics 89 (2), 215-223 (2007)
   PUBMED   17049203
  REMARK    GeneRIF: phylogenetic analyses show that the BRDT germ
            cell-specific orthology group clearly evolves faster than its
            ubiquitously expressed paralogs BRD2, BRD3, and BRD4
REFERENCE   7  (bases 1 to 4710)
  AUTHORS   Govin,J., Lestrat,C., Caron,C., Pivot-Pajot,C., Rousseaux,S. and
            Khochbin,S.
  TITLE     Histone acetylation-mediated chromatin compaction during mouse
            spermatogenesis
  JOURNAL   Ernst Schering Res Found Workshop (57), 155-172 (2006)
   PUBMED   16568954
REFERENCE   8  (bases 1 to 4710)
  AUTHORS   Shang,E., Salazar,G., Crowley,T.E., Wang,X., Lopez,R.A., Wang,X.
            and Wolgemuth,D.J.
  TITLE     Identification of unique, differentiation stage-specific patterns
            of expression of the bromodomain-containing genes Brd2, Brd3, Brd4,
            and Brdt in the mouse testis
  JOURNAL   Gene Expr Patterns 4 (5), 513-519 (2004)
   PUBMED   15261828
REFERENCE   9  (bases 1 to 4710)
  AUTHORS   Pivot-Pajot,C., Caron,C., Govin,J., Vion,A., Rousseaux,S. and
            Khochbin,S.
  TITLE     Acetylation-dependent chromatin reorganization by BRDT, a
            testis-specific bromodomain-containing protein
  JOURNAL   Mol Cell Biol 23 (15), 5354-5365 (2003)
   PUBMED   12861021
REFERENCE   10 (bases 1 to 4710)
  AUTHORS   Taniguchi,Y., Suzuki,H., Ohtsuka,M., Kikuchi,N., Kimura,M. and
            Inoko,H.
  TITLE     Isolation and characterization of three genes paralogous to mouse
            Ring3
  JOURNAL   Nucleic Acids Res Suppl (1), 247-248 (2001)
   PUBMED   12836357
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF358660.1, BY726053.1,
            BY724250.1, AK039465.1 and AK078663.1.
            
            On Dec 29, 2006 this sequence version replaced NM_054054.1.
            
            Summary: The protein encoded by this gene belongs to the BET
            protein family. BET proteins have two N-terminal bromodomains and
            one C-terminal extraterminal domain (ET domain). BET proteins
            regulate chromatin reorganization via binding to acetylated
            histones. This gene is thought to play a role in the
            transcriptional regulation of spermatogenesis. Although referred to
            as testis-specific bromodomain (Brdt) protein, RT-PCR indicates
            that this gene is expressed in both mouse oocytes and testes.
            Alternative splicing results in multiple transcript variants
            encoding different proteins. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF358660.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-971               AF358660.1         36-1006
            972-972             BY726053.1         298-298
            973-2378            AF358660.1         1008-2413
            2379-2379           BY724250.1         145-145
            2380-3347           AF358660.1         2415-3382
            3348-3377           AK039465.1         905-934
            3378-4710           AK078663.1         869-2201
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 52.23 cM"
     gene            1..4710
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /note="bromodomain, testis-specific"
                     /db_xref="GeneID:114642"
                     /db_xref="MGI:MGI:1891374"
     exon            1..114
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            115..256
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            257..496
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..3178
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /note="isoform A is encoded by transcript variant 1;
                     bromodomain testis-specific protein; RING3-like protein;
                     bromodomain-containing female sterile homeotic-like
                     protein; female sterile homeotic-related gene 3"
                     /codon_start=1
                     /product="bromodomain testis-specific protein isoform A"
                     /protein_id="NP_473395.2"
                     /db_xref="CCDS:CCDS19500.1"
                     /db_xref="GeneID:114642"
                     /db_xref="MGI:MGI:1891374"
                     /translation="MSLPSRQTAIVNPPPPEYINTKKSGRLTNQLQFLQRVVLKALWK
                     HGFSWPFQQPVDAVKLKLPDYYTIIKTPMDLNTIKKRLENKYYEKASECIEDFNTMFS
                     NCYLYNKTGDDIVVMAQALEKLFMQKLSQMPQEEQVVGGKERIKKDIQQKIAVSSAKE
                     QIPSKAAENVFKRQEIPSGLPDISLSPLNMAQEAPPICDSQSLVQITKGVKRRADTTT
                     PTTSIAKASSESPPTLRETKPVNMPVKENTVKNVLPDSQQQHKVLKTVKVTEQLKHCS
                     EILKEMLAKKHLPYAWPFYNPVDADALGLHNYYDVVKNPMDLGTIKGKMDNQEYKDAY
                     EFAADVRLMFMNCYKYNPPDHEVVAMARTLQDVFELHFAKIPDEPIESMHACHLTTNS
                     AQALSRESSSEASSGDASSEDSEDERVQHLAKLQEQLNAVHQQLQVLSQVPLRKLKKK
                     NEKSKRAPKRKKVNNRDENPRKKPKQMKQKEKAKINQPKKKKPLLKSEEEDNAKPMNY
                     DEKRQLSLDINKLPGDKLGRIVHIIQSREPSLRNSNPDEIEIDFETLKASTLRELEKY
                     VLACLRKRSLKPQAKKVVRSKEELHSEKKLELERRLLDVNNQLNCRKRQTKRPAKVEK
                     PPPPPPPPPPPPPPPELASGSRLTDSSSSSGSGSGSSSSSSGSSSSSSSSGSASSSSD
                     SSSSDSSDSEPEIFPKFTGVKQNDLPPKENIKQIQSSVQDITSAEAPLAQQSTAPCGA
                     PGKHSQQMLGCQVTQHLQATENTASVQTQPLSGDCKRVLLGPPVVHTSAESLTVLEPE
                     CHAPAQKDIKIKNADSWKSLGKPVKASSVLKSSDELFNQFRKAAIEKEVKARTQEQMR
                     KHLEHNAKDPKVSQENQREPGSGLTLESLSSKVQDKSLEEDQSEQQPPSEAQDVSKLW
                     LLKDRNLAREKEQERRRREAMAGTIDMTLQSDIMTMFENNFD"
     exon            497..634
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            635..749
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            750..922
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1273
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1402
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1591
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1764
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..2045
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2171
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            2172..2387
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2449
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            2450..2624
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            2625..2722
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            2723..2928
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..3109
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     exon            3110..4710
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3975..3980
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /note="hexamer: AATGAA"
     polyA_site      4020
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /note="major polyA site"
     regulatory      4102..4107
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
                     /note="hexamer: AATATA"
     polyA_site      4122
                     /gene="Brdt"
                     /gene_synonym="7420412D09Rik; Brd6; Fsrg3"
ORIGIN      
        1 ccgccatccg ggcgagagct ggcggccacc ggtggagaac cgggagcgac ggtcctgcac
       61 tgcttcatct agtgcaaact gaaagggtga tcggataagg ccctttccta agaggaattt
      121 gtagactttt cctgctaaga cctcaatttg tatttttgaa gaacagaaag aaattctgtg
      181 acgtgttgaa ataatttaaa ccatctaaga ccaagacaca cataatgaag caatgcatac
      241 tatttatata aaaaaggaca tgtcctctta gacaacttta aaataccagg atccgaagtg
      301 gttgagaatg tctctgccaa gtcgacaaac agctattgtt aacccccctc caccagagta
      361 cataaacact aagaagagtg ggcggttgac gaatcagctg cagttcctac agagggttgt
      421 gctgaaggcc ctgtggaagc acggcttctc ttggcctttc caacagccgg tggacgccgt
      481 gaaactaaag ctgcctgact attacaccat cataaaaacc ccaatggatt taaatacaat
      541 taagaagcgg ttggaaaata agtattatga gaaggcttcc gaatgcatag aagacttcaa
      601 cacaatgttc tcaaactgct acttatacaa caagactgga gatgacattg ttgttatggc
      661 ccaagctcta gagaagttat ttatgcagaa attatctcag atgccacaag aagagcaagt
      721 tgtgggtggt aaggagagaa taaagaaaga tatccaacaa aagatagcgg tttcctctgc
      781 taaggaacaa atcccctcca aagcggcaga gaatgtcttt aagcggcaag agattccttc
      841 tgggcttcct gacatctctc tctcgccctt aaacatggca caagaagctc ccccgatctg
      901 tgactcgcag tctctggtcc aaattacaaa gggtgtgaag aggcgagcag acacaacaac
      961 tcctaccact tccatagcta aagcaagtag cgaatctcct ccgacactta gagaaacaaa
     1021 gccagtgaac atgccagtaa aagaaaatac agtaaagaac gttttgccag actctcagca
     1081 acaacacaaa gttttaaaaa cagtcaaagt aacggagcaa ttaaagcact gcagcgagat
     1141 tcttaaagaa atgcttgcca agaaacattt gccgtatgca tggcccttct ataatcctgt
     1201 ggatgctgat gctttgggac tccacaacta ctatgacgtt gtcaaaaatc ccatggatct
     1261 cggaacgatc aaggggaaaa tggacaatca agaatataag gatgcatatg aatttgctgc
     1321 agatgtcaga ctgatgttca tgaactgtta caagtataat cctcccgatc atgaagttgt
     1381 ggcgatggct cggacccttc aggatgtttt tgaactacat tttgccaaga ttcccgatga
     1441 acctattgag agtatgcatg catgccacct tacaacaaac agtgcacaag ccctcagcag
     1501 agaaagcagc agcgaggcct cctcggggga cgcctcttct gaagactctg aggatgaacg
     1561 agtgcagcat cttgccaagc ttcaggagca gcttaatgct gttcatcagc agctgcaagt
     1621 tctgtcccaa gttccattac gtaagctgaa gaaaaagaat gagaagtcca aaagggcgcc
     1681 aaaaaggaaa aaggttaata acagagatga aaacccaagg aaaaagccta agcaaatgaa
     1741 gcaaaaggag aaggccaaaa tcaatcaacc aaagaagaag aagccgcttt tgaaatcgga
     1801 agaggaagac aatgcgaagc ctatgaacta cgatgagaaa aggcagctga gcttggacat
     1861 aaacaaactg cctggagaca agcttggtcg aatagttcac ataatacagt caagggagcc
     1921 ttccctgaga aactccaacc cggacgagat cgagatcgac tttgagaccc tgaaggcatc
     1981 cacacttaga gaactggaga agtatgtcct ggcctgtctg aggaagaggt cgctgaagcc
     2041 ccaagcaaag aaggttgtca ggtctaagga ggagctccat tctgaaaaaa agctggagct
     2101 ggagaggcgg ttgctggacg tcaacaatca gctgaattgt aggaaacggc aaacgaaacg
     2161 tccagcaaaa gtggagaagc cgcccccgcc cccgcccccg cccccgcccc cgccgcctcc
     2221 cccggagctg gccagtgggt ctcgcctgac cgacagcagc agcagcagcg gcagcggcag
     2281 cggcagcagc agcagcagca gcggcagcag cagcagcagc agctcctcgg ggtccgcgag
     2341 cagcagcagc gactcgagct cctccgacag cagcgattct gaaccagaaa tatttcctaa
     2401 atttactgga gtaaaacaga atgatttacc tccaaaagaa aatataaagc agatacaatc
     2461 ttctgtgcaa gacataacct ctgctgaagc tccccttgct cagcagagca cagcaccatg
     2521 cggagcccca gggaagcact cccagcagat gcttggttgc caagtgacac agcatctgca
     2581 ggccacagag aacactgcat ctgtgcagac tcagcctctt tcaggtgatt gcaagcgagt
     2641 attgcttggc ccccctgtgg tgcacacctc tgctgagagc ctcacagtgc tggagccaga
     2701 atgccatgct cccgcgcaga aggatataaa gattaagaat gcagactctt ggaaaagttt
     2761 agggaagcca gtgaaagcat caagtgtact gaagtcttca gatgagcttt tcaaccagtt
     2821 tagaaaagct gcaatagaaa aggaggtaaa ggcccggaca caggagcaga tgcggaaaca
     2881 cctggagcat aatgcgaagg acccaaaagt atctcaagaa aatcagaggg agcctggaag
     2941 tggattgact ctagaatctt tatcaagtaa agtgcaagat aagtctcttg aagaagatca
     3001 gagtgaacag cagccaccgt cggaagctca agacgtaagc aagctctggc ttctcaaaga
     3061 ccggaatcta gcgagggaga aagagcaaga gcgcaggaga agagaagcaa tggcgggtac
     3121 cattgatatg actcttcaaa gtgacattat gacaatgttt gaaaataact ttgattaaaa
     3181 ctcagtttgt aaattaaccc tcaactttaa atgaaaggta aaagtgttta cagtgggcgt
     3241 ggttaaagtg gttactttaa acaccagaat gtcaatcact gattgtgttc tgactgccct
     3301 aaataatttt tcatttacat tttaataaat atgttctttt aatgacataa ttgataaaat
     3361 gttttataat tttgacctct gaaagtggat agattttatt catcctaaag aggactgtca
     3421 gtgatgtaat atagtcctga tctaagtcta aaagggatca tcagattgaa aacaaatgaa
     3481 gaatacataa aagtatataa aacacgtttt gttgagattt taacttttgt tttttgtcta
     3541 aacctgtttg ttggttagtt ggtttgtgtc cttgtttgag acagggtctc atatactcag
     3601 gctggctttg gtgaactcaa catgtagcca aggctggcct tgatctcctg attctcctgc
     3661 cctcagctct caagtactgg actccagaag tgtgtcttca tgcctggctg agttgttaac
     3721 ttttcaaaga gatatgtgtc tgctgtcaga attgtaacag aacttctaac aagaatactt
     3781 ggtttccatg tgagtcacag tgtgtcagat tctctgctag ttccatgttt aaattccata
     3841 ctgccttttg ttttaaagta atattagcta tgatttgaaa ggtgttaaat tttaagcaaa
     3901 gtaatagtaa atattgtgta tacaagcttc tgtgggaatg tgtcagacat ttcttaaaat
     3961 gtgtaggtga tagcaatgaa tatttgccta accaggggaa attatagcct atgaaacatt
     4021 gatgttttgt catttttcaa actgcatgca tgtctaacca tatgtaatct tttttaaaat
     4081 tttaaacctt tgaacatttg aaatataacc tgattaaggc aatgctgtga tctaaagtac
     4141 aaactcagtg tgtgtccaga cagggaagtg cacacctgct gtccagacag ggcagtgcac
     4201 acctgctatg cagacagggc agtgcacatc tgctgtccag acagggcagt gcacacctgc
     4261 tatgcagaca gggcagtgca cacctgctgt ccagacaggg cagtgcacac ctgctatgta
     4321 gacagggcag tgcacacctg ctgtccagac agggcagtgc acacctgctg tccagacagg
     4381 gcagtgcaca cctgctgtcc agacagggca gtgaacacct gctgtccaga cagggcagtg
     4441 cacacctgct gtaattccag tatttggggg gcagaggcag agaaggagtc actgctgata
     4501 actcaagacc agcctggttc acatagcaat cttcagattc tggtcatagc cagacctcct
     4561 tgattgagaa gaaccaagaa agagagagag aaagagagta agaagatggt attgctttca
     4621 ggaaacattg ctttcaggaa acaaccagaa ttaaagtgat agatattttg ttgtacctat
     4681 tttctacttt ttaattaaaa actttatatg
//
