LOCUS       NM_020508               5948 bp    mRNA    linear   ROD 07-JAN-2025
DEFINITION  Mus musculus bromodomain containing 4 (Brd4), transcript variant 1,
            mRNA.
ACCESSION   NM_020508
VERSION     NM_020508.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Li,C., Zhang,M., Du,Y., Liu,S., Li,D., Zhang,S., Ji,F., Zhang,J.
            and Jiao,J.
  TITLE     Compromised cell competition exhausts neural stem cells pool
  JOURNAL   Cell Prolif 57 (12), e13710 (2024)
   PUBMED   39010274
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Huang,C., Liu,Z., Chen,M., Zhang,H., Mo,R., Chen,R., Liu,Y.,
            Wang,S. and Xue,Q.
  TITLE     Up-regulation of BRD4 contributes to gestational diabetes
            mellitus-induced cardiac hypertrophy in offspring by promoting
            mitochondria dysfunction in sex-independent manner
  JOURNAL   Biochem Pharmacol 226, 116387 (2024)
   PUBMED   38944397
  REMARK    GeneRIF: Up-regulation of BRD4 contributes to gestational diabetes
            mellitus-induced cardiac hypertrophy in offspring by promoting
            mitochondria dysfunction in sex-independent manner.
REFERENCE   3  (bases 1 to 5948)
  AUTHORS   Zhang,F., Zhou,J., Lu,P., Zhang,X., Yang,L., Wu,J., Zhang,L.,
            Zhang,L., Pang,J., Xie,H., Xie,B., Jiang,Y. and Peng,J.
  TITLE     Lactylation of histone by BRD4 regulates astrocyte polarization
            after experimental subarachnoid hemorrhage
  JOURNAL   J Neuroinflammation 21 (1), 186 (2024)
   PUBMED   39080649
  REMARK    GeneRIF: Lactylation of histone by BRD4 regulates astrocyte
            polarization after experimental subarachnoid hemorrhage.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5948)
  AUTHORS   Chen,F., Li,S., Liu,M., Qian,C., Shang,Z., Song,X., Jiang,W. and
            Tu,C.
  TITLE     Targeting BRD4 mitigates hepatocellular lipotoxicity by suppressing
            the NLRP3 inflammasome activation and GSDMD-mediated hepatocyte
            pyroptosis
  JOURNAL   Cell Mol Life Sci 81 (1), 295 (2024)
   PUBMED   38977508
  REMARK    GeneRIF: Targeting BRD4 mitigates hepatocellular lipotoxicity by
            suppressing the NLRP3 inflammasome activation and GSDMD-mediated
            hepatocyte pyroptosis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5948)
  AUTHORS   Padmanabhan,A., de Soysa,T.Y., Pelonero,A., Sapp,V., Shah,P.P.,
            Wang,Q., Li,L., Lee,C.Y., Sadagopan,N., Nishino,T., Ye,L., Yang,R.,
            Karnay,A., Poleshko,A., Bolar,N., Linares-Saldana,R., Ranade,S.S.,
            Alexanian,M., Morton,S.U., Jain,M., Haldar,S.M., Srivastava,D. and
            Jain,R.
  TITLE     A genome-wide CRISPR screen identifies BRD4 as a regulator of
            cardiomyocyte differentiation
  JOURNAL   Nat Cardiovasc Res 3 (3), 317-331 (2024)
   PUBMED   39196112
  REMARK    GeneRIF: A genome-wide CRISPR screen identifies BRD4 as a regulator
            of cardiomyocyte differentiation.
REFERENCE   6  (bases 1 to 5948)
  AUTHORS   Maruyama,T., Farina,A., Dey,A., Cheong,J., Bermudez,V.P.,
            Tamura,T., Sciortino,S., Shuman,J., Hurwitz,J. and Ozato,K.
  TITLE     A Mammalian bromodomain protein, brd4, interacts with replication
            factor C and inhibits progression to S phase
  JOURNAL   Mol Cell Biol 22 (18), 6509-6520 (2002)
   PUBMED   12192049
  REMARK    GeneRIF: brd4 binds to replication factor C and inhibits
            progression to S phase
REFERENCE   7  (bases 1 to 5948)
  AUTHORS   Houzelstein,D., Bullock,S.L., Lynch,D.E., Grigorieva,E.F.,
            Wilson,V.A. and Beddington,R.S.
  TITLE     Growth and early postimplantation defects in mice deficient for the
            bromodomain-containing protein Brd4
  JOURNAL   Mol Cell Biol 22 (11), 3794-3802 (2002)
   PUBMED   11997514
  REMARK    GeneRIF: Growth and early postimplantation defects in mice
            deficient for the bromodomain-containing protein Brd4.
REFERENCE   8  (bases 1 to 5948)
  AUTHORS   Banu,Y., Matsuda,M., Yoshihara,M., Kondo,M., Sutou,S. and
            Matsukuma,S.
  TITLE     Golgi matrix protein gene, Golga3/Mea2, rearranged and re-expressed
            in pachytene spermatocytes restores spermatogenesis in the mouse
  JOURNAL   Mol Reprod Dev 61 (3), 288-301 (2002)
   PUBMED   11835574
REFERENCE   9  (bases 1 to 5948)
  AUTHORS   Dey,A., Ellenberg,J., Farina,A., Coleman,A.E., Maruyama,T.,
            Sciortino,S., Lippincott-Schwartz,J. and Ozato,K.
  TITLE     A bromodomain protein, MCAP, associates with mitotic chromosomes
            and affects G(2)-to-M transition
  JOURNAL   Mol Cell Biol 20 (17), 6537-6549 (2000)
   PUBMED   10938129
REFERENCE   10 (bases 1 to 5948)
  AUTHORS   Resch,K., Korthaus,D., Wedemeyer,N., Lengeling,A., Ronsiek,M.,
            Thiel,C., Baer,K., Jockusch,H. and Schmitt-John,T.
  TITLE     Homology between human chromosome 2p13.3 and the wobbler critical
            region on mouse chromosome 11: comparative high-resolution mapping
            of STS and EST loci on YAC/BAC contigs
  JOURNAL   Mamm Genome 9 (11), 893-898 (1998)
   PUBMED   9799840
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY779550.1, AK147545.1 and CJ192204.1.
            
            On Nov 8, 2013 this sequence version replaced NM_020508.3.
            
            Transcript Variant: This variant (1) has two alternate splice
            sites, one in the 5' UTR and one in the 5' coding region, compared
            to variant 3. The resulting isoform (1) lacks an internal aa,
            compared to isoform 3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF461395.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                BY779550.1         2-40
            40-231              AK147545.1         5-196
            232-824             AK147545.1         241-833
            825-5944            AK147545.1         837-5956
            5945-5948           CJ192204.1         447-450
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 17.39 cM"
     gene            1..5948
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /note="bromodomain containing 4"
                     /db_xref="GeneID:57261"
                     /db_xref="MGI:MGI:1888520"
     exon            1..71
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            72..231
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            232..550
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     CDS             266..4468
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /EC_number="3.1.1.31"
                     /note="isoform 1 is encoded by transcript variant 1;
                     bromodomain-containing protein 4; fsh-like protein;
                     mitotic chromosome-associated protein;
                     bromodomain-containing 5"
                     /codon_start=1
                     /product="bromodomain-containing protein 4 isoform 1"
                     /protein_id="NP_065254.3"
                     /db_xref="CCDS:CCDS50057.1"
                     /db_xref="GeneID:57261"
                     /db_xref="MGI:MGI:1888520"
                     /translation="MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTN
                     PPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIK
                     TPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQK
                     INELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPP
                     VQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPII
                     ATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKK
                     DVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYC
                     DIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDV
                     FEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEER
                     AQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKT
                     KELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDIN
                     KLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKP
                     QAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHH
                     HHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSS
                     PPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQH
                     AVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVL
                     LEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQ
                     LQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQ
                     PPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPP
                     QQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVV
                     KEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQS
                     APPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEH
                     FRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQE
                     QQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREA
                     MAATIDMNFQSDLLSIFEENLF"
     exon            551..688
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            689..824
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            825..1117
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1480
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1609
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1819
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..2019
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2315
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2426
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            2427..2479
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            2480..2852
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..3443
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            3444..3818
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            3819..3949
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            3950..4155
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            4156..4399
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     exon            4400..5948
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5923..5928
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /note="hexamer: AATAAA"
     polyA_site      5946
                     /gene="Brd4"
                     /gene_synonym="Brd5; HUNK1; MCAP; WI-11513"
                     /note="major polyA site"
ORIGIN      
        1 aagtgaggag gaggaggagg aggaggagga ggaagcgact gcctctggat ctgcggtgcg
       61 cgccgggccg cccaacaaga tagctgtgtt ccagttttgt tcaaaactac tagtgaagaa
      121 gtcctcctct cctctcattc ccatatctgc tttccgtctg gacacaacat tgggtctaag
      181 aaaatcagct caccaggctg tgaccactga atcacattgg agttctctgt aagtgcctgg
      241 tgaagaatgt gatgggatca ctagcatgtc tacggagagc ggccctggga caagattgag
      301 aaatctgcca gtaatggggg atggactaga aacctcccaa atgtctacaa cgcaggccca
      361 ggcccaaccc cagccagcaa atgcagccag caccaatcct ccacccccag agacctccaa
      421 ccctaacaag cccaagagac agacaaacca actgcaatat ctgctcagag tggtgctcaa
      481 gacactatgg aaacaccagt ttgcgtggcc tttccagcag cccgtggatg ccgtcaagct
      541 gaacctccct gattactata agattattaa aacacccatg gatatgggaa caataaagaa
      601 gcgcttggaa aacaactatt actggaatgc tcaggaatgt atccaggact tcaacactat
      661 gtttacaaat tgttacatct ataacaagcc tggagatgac atcgtcttaa tggcagaagc
      721 tctggagaag ctcttcttgc aaaaaatcaa tgaactgcct acagaagaaa ctgagatcat
      781 gatagtccag gcaaaaggaa gaggacgagg gaggaaagaa acaggggcag caaagcctgg
      841 tgtatccacg gtaccaaaca caactcaagc atcaacttct ccgcagaccc agacgcctca
      901 gcagaaccct cctccacctg tgcaggccac aactcacccc tttcctgctg tcaccccaga
      961 cctcattgcc cagcctcctg tcatgacaat ggtgccccct cagccacttc agactccttc
     1021 accggtaccc ccccagccac cacccccacc tgctccagtt ccacagcctg tgcagagtca
     1081 cccgcccatc attgcgacca ccccccagcc tgtgaagaca aagaaagggg tgaagaggaa
     1141 agcagatacc accaccccta ccaccatcga ccccattcat gagccaccct cactggcccc
     1201 agagcccaag accgccaagc tgggtcctcg gcgggagagc agcagacctg tgaagcctcc
     1261 aaagaaggat gtaccggact cacagcagca cccagggcca gagaagagca gcaagatctc
     1321 tgagcagcta aagtgctgca gtggcatcct caaggagatg tttgccaaga aacatgctgc
     1381 ctatgcctgg cctttctaca agcctgtgga tgtggaggca ctgggtctgc acgactactg
     1441 tgacatcatc aaacatccca tggacatgag cacaatcaag tctaaactag agtcccgaga
     1501 gtacagagat gcccaggaat ttggtgctga tgtccgattg atgttctcca actgctacaa
     1561 gtacaacccc cctgaccatg aagtggtagc catggctcga aaactccagg atgtgtttga
     1621 aatgcgcttt gccaagatgc ctgatgagcc tgaagagcca gttgttacag tgtcctctcc
     1681 tgcagtgcca ccccctacaa aggtggtagc cccaccctca tctagtgaca gcagcagcga
     1741 cagttcttcc gacagcgaca gttccactga cgactctgag gaagagcgag cccagcggct
     1801 ggctgaactc caggaacagc tcaaggccgt gcatgagcag cttgcagccc tctcacagcc
     1861 ccagcagaac aaaccaaaga aaaaggagaa ggacaagaag gaaaagaaaa aggaaaagca
     1921 caaaaagaaa gaagaagtgg aggaaaataa aaaaagcaaa accaaggaac ttcctcccaa
     1981 aaagacaaag aaaaataaca gcagcaacag caatgtgagc aagaaggaac cagtacccac
     2041 gaagaccaag ccgcctccca catatgaatc agaagaggag gataagtgta agcccatgtc
     2101 ttatgaggag aagcggcagc taagtctaga tatcaacaaa cttcctggtg agaagctagg
     2161 ccgtgtagta cacataattc agtcaaggga accatcactt aaaaactcca accccgatga
     2221 gattgagatt gactttgaga ccctgaagcc atctacacta cgagagttgg agcgatatgt
     2281 cacctcctgt ttgcggaaga aaaggaaacc tcaagctgaa aaagttgacg tgattgctgg
     2341 ttcttccaag atgaagggat tctcatcctc tgagtcggag agcaccagcg aatccagctc
     2401 ctctgacagt gaagactctg aaacagagat ggctcccaag tcaaaaaaga aggggcacac
     2461 tgggagggac cagaaaaagc atcatcacca tcaccatcca cagatgcagc cagccccagc
     2521 tcctgtgccc cagcagccgc ccccacctcc acagcagcct cctccacccc cacctccgca
     2581 gcagcagcag cagcaacctc cacccccacc acctccgccc tccatgccac agcagactgc
     2641 cccagcgatg aagtcctcgc ccccaccctt catcactgcc caggtccccg tcctggaacc
     2701 acagctgcca ggcagtgtct ttgaccctat tggccacttc acccagccca tcttgcacct
     2761 gccgcagccg gagctgcctc ctcacctgcc ccagccacct gagcacagca ctccacccca
     2821 tctcaaccag catgctgtgg tctctcctcc agctttgcac aatgcgctgc cccaacagcc
     2881 atctcggccc agtaaccgag ctgctgctct gcccccaaag cctacccgac ccccagctgt
     2941 gtcccctgcc ctggcccagc cccccctgct cccacaacca ccaatggctc agccccccca
     3001 agtgctgctg gaggatgaag agccacctgc cccacccctc acctccatgc agatgcagtt
     3061 gtacctacag cagctgcaga aggtgcagcc tcccacacca ctactccctt ccgtgaaggt
     3121 gcagtcccaa cccccaccgc ctttgccgcc tccaccccac ccttctgtgc agcagcagca
     3181 gctccagcca cagccaccgc cacccccacc tcctcagcca cagccaccac cccagcaaca
     3241 acaccagcct ccaccacgac cagttcactt gccatccatg cccttttctg ctcatattca
     3301 gcagccccca ccacccccag gacagcaacc tactcaccca cccccaggac agcagccccc
     3361 accaccacag cctgccaagc cccagcaagt catccagcat cacccttccc cccggcacca
     3421 caagtcagac ccctactcag ctggtcatct tcgtgaggct ccctctccgc ttatgataca
     3481 ttcccctcag atgccacagt tccagagcct gacccatcag tctcctcccc agcaaaacgt
     3541 ccagcctaag aagcaggtaa agggcagggc tgagccacag ccaccagggc cagtcatggg
     3601 ccaaggccag ggatgcccac ctgcctcacc ggctgccgtg cctatgctgt cccaggagct
     3661 gcgaccacct tcagtcgtcc agccccagcc cctggtggta gtaaaggagg agaagattca
     3721 ctcaccaatc attcgcagcg agcctttcag cacctcactt cgaccagagc cccccaagca
     3781 cccagagaac atcaaggccc cagtccacct gccccagcgg cctgagatga agcctgtaga
     3841 catagggagg cctgtgatcc ggcctccaga gcagagtgca cccccaccag gggcccctga
     3901 caaggacaaa cagaagcagg agccaaaaac accagtggca cccaaaaagg acctgaaaat
     3961 taagaacatg ggctcctggg ccagcctggt acagaagcat ccgaccaccc catcctccac
     4021 agccaagtcc tcaagtgaca gctttgagca tttccgccgt gctgctcggg agaaggagga
     4081 gagggaaaag gccctgaagg ctcaggctga gcatgcagag aaggagaagg agcggctgag
     4141 gcaggagcgc atgaggagcc gagaggatga agatgcgctg gaacaggcac gtcgagccca
     4201 tgaggaggca cgacggcgcc aggagcagca gcagcagcag cagcagcaga gacaggagca
     4261 gcagcagcag cagcagcagg cagctgctgt ggccgctgcc tctgcccccc aggcccagag
     4321 ctcccagccc cagtctatgc tggaccagca gagggagttg gcccggaaac gagagcagga
     4381 gcggaggcgc agggaggcaa tggcagctac aattgacatg aatttccaga gtgatctttt
     4441 gtcaatattt gaagaaaatc ttttttgaga gcacccaggt gacttctgac tttttctggc
     4501 aaaaatgttg actctccatc catagtgtta ggggcagccg tggaagcagc agcagccagg
     4561 gatgtctctg ggcctggctc tcctgcatgc tgtgcccggg gcaggcctga cgggcgggca
     4621 gctgaggatc gcagagccca tctgccttac agccagccgg acagacgtcc tactacatgc
     4681 caccccctca gaggacgtca gttcagggca cgtctcaata tgagtcaagt gccagctgga
     4741 cctgaggcct gcttccccgc gtgcggggca gaggcccttc tctgcgccaa atgtctgact
     4801 acacagtata cacaggacat tgttgctgcc gccaccgaga ctggttttct gtccctgaga
     4861 acatgacgtt tgtgacgtcc tacccattgg gagtccttca caccccagcc atcgctgccc
     4921 actcccagga ggccagggca agcctgtgtg gactggagca gcaaggcact ggcccacccc
     4981 ttgctggcac tgactttgcc ttgaacagac cctctcccct cccccacaag cctttaattg
     5041 agagccgctc tttataagtg ttcgcttgtg caaaagggaa tagtgccgtg gaagtgtgtg
     5101 tccatggcag tttggacagg ggtgactgtt cacacgtatg gacaggcctc gcctggggag
     5161 taaggccacc aaccaaagtc agttccttcc cacctgtgtt tctgattttg ttttgtttta
     5221 tttttttcct atatatatat ttttttttgt tgttgttgaa ttctatttta tttttaactc
     5281 tcttctcctc agacacagtg gcactgctta tctccaaatg gtgtgatcgt ctcctcagtg
     5341 agcggcggct cccactgcgc tgtgggtagt gtgtgactgt ggctgtactg tatagtgaac
     5401 atagttggca tatctttgtt tgaagtttgt tggtgattcc accaaactgg tgtaaaaaac
     5461 aaaaacaaaa aaacccacaa accacccaaa actcacaaaa aaaatcctgc ttgtattcag
     5521 tttcaaactt tattagtctc acttttaatt ataaaaccag aaagctgcaa tttcttttcc
     5581 ctctacccct tatttgttgg cttctttgtt tttttaatgt caaatctgtt tgtttttacc
     5641 aagaaaggga gagcctgagg aggtctgcag gctttggtgg gctgcgaacc gaggcggagg
     5701 cccccaccca gtacctggtg agaccagtgt gctgcgcctt ccttcctatt agtatttttg
     5761 tttcgttttg tttttaaatt ggagcccctg gtgaggttac atgtgccatg agaacccact
     5821 ctacaccata gtgctggtgc ctcagtgttg gccaaactct ggagtcactg actggtttga
     5881 ctttcatgtg gtaaatatgc atttggtctg tactgatcat ggaataaaca catctctctt
     5941 ttttaatg
//
