LOCUS       NM_028227               3737 bp    mRNA    linear   ROD 01-JUL-2024
DEFINITION  Mus musculus BRCA1 associated protein (Brap), transcript variant 1,
            mRNA.
ACCESSION   NM_028227
VERSION     NM_028227.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Zheng,J., Wang,H., Wang,J., Peng,Z., Yao,X., Weber,H.C., Qin,X.,
            Xiang,Y., Liu,C., Ji,M., Liu,H. and Qu,X.
  TITLE     Bombesin receptor-activated protein homolog deficiency altered the
            pattern of pathological changes of psoriasis - like skin lesion in
            mice
  JOURNAL   Int J Med Sci 21 (2), 357-368 (2024)
   PUBMED   38169666
  REMARK    GeneRIF: Bombesin receptor-activated protein homolog deficiency
            altered the pattern of pathological changes of psoriasis - like
            skin lesion in mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3737)
  AUTHORS   Wang,H., Zuo,S., Zheng,J., Peng,Z., Yao,X., Wang,J., Weber,H.C.,
            Qin,X., Xiang,Y., Liu,C., Ji,M., Liu,H., Pan,L. and Qu,X.
  TITLE     Knockout of the BRAP homolog in mice leads to abnormal tracheal
            cilia
  JOURNAL   FEBS Lett 597 (21), 2626-2642 (2023)
   PUBMED   37715941
  REMARK    GeneRIF: Knockout of the BRAP homolog in mice leads to abnormal
            tracheal cilia.
REFERENCE   4  (bases 1 to 3737)
  AUTHORS   Guo,Y., Chomiak,A.A., Hong,Y., Lowe,C.C., Kopsidas,C.A., Chan,W.C.,
            Andrade,J., Pan,H., Zhou,X., Monuki,E.S. and Feng,Y.
  TITLE     Histone H2A ubiquitination resulting from Brap loss of function
            connects multiple aging hallmarks and accelerates neurodegeneration
  JOURNAL   iScience 25 (7), 104519 (2022)
   PUBMED   35754718
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3737)
  AUTHORS   Chomiak,A.A., Guo,Y., Kopsidas,C.A., McDaniel,D.P., Lowe,C.C.,
            Pan,H., Zhou,X., Zhou,Q., Doughty,M.L. and Feng,Y.
  TITLE     Nde1 is required for heterochromatin compaction and stability in
            neocortical neurons
  JOURNAL   iScience 25 (6), 104354 (2022)
   PUBMED   35601919
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3737)
  AUTHORS   Fatima,S., Wagstaff,K.M., Loveland,K.L. and Jans,D.A.
  TITLE     Interactome of the negative regulator of nuclear import
            BRCA1-binding protein 2
  JOURNAL   Sci Rep 5, 9459 (2015)
   PUBMED   25820252
  REMARK    GeneRIF: Together the results indicate for the first time that
            BRAP2 may play an important NRNI role in germ cells of the testis,
            with an additional, scaffold/structural role in mature spermatozoa.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3737)
  AUTHORS   Davies,R.G., Wagstaff,K.M., McLaughlin,E.A., Loveland,K.L. and
            Jans,D.A.
  TITLE     The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention
            factor for nuclear and nuclear envelope-localizing testicular
            proteins
  JOURNAL   Biochim Biophys Acta 1833 (12), 3436-3444 (2013)
   PUBMED   23707952
  REMARK    GeneRIF: ectopic expression of BRAP2 inhibits nuclear localization
            of HMG20A and NuMA1, and prevents nuclear envelope accumulation of
            SYNE2.
REFERENCE   8  (bases 1 to 3737)
  AUTHORS   Lanctot,A.A., Peng,C.Y., Pawlisz,A.S., Joksimovic,M. and Feng,Y.
  TITLE     Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap
            complex patterns mammalian CNS
  JOURNAL   Dev Cell 25 (3), 241-255 (2013)
   PUBMED   23673330
  REMARK    GeneRIF: A molecular complex composed of Nde1, Lis1, and Brap
            regulates the dynamic MAPK signaling threshold in a spatially
            dependent fashion.
REFERENCE   9  (bases 1 to 3737)
  AUTHORS   Czyzyk,J., Chen,H.C., Bottomly,K. and Flavell,R.A.
  TITLE     p21 Ras/impedes mitogenic signal propagation regulates cytokine
            production and migration in CD4 T cells
  JOURNAL   J Biol Chem 283 (34), 23004-23015 (2008)
   PUBMED   18577512
  REMARK    GeneRIF: p21 Ras/Imp regulate cytokine production and migration in
            CD4 T cells
REFERENCE   10 (bases 1 to 3737)
  AUTHORS   Nakajima,A., Kataoka,K., Hong,M., Sakaguchi,M. and Huh,N.H.
  TITLE     BRPK, a novel protein kinase showing increased expression in mouse
            cancer cell lines with higher metastatic potential
  JOURNAL   Cancer Lett 201 (2), 195-201 (2003)
   PUBMED   14607334
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY739796.1, BC062932.1 and AC116104.7.
            
            On Jan 23, 2014 this sequence version replaced NM_028227.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062932.1, AF321920.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BY739796.1         2-24
            24-560              BC062932.1         1-537
            561-562             AC116104.7         128303-128304       c
            563-3115            BC062932.1         539-3091
            3116-3737           AC116104.7         104463-105084       c
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 61.9 cM"
     gene            1..3737
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /note="BRCA1 associated protein"
                     /db_xref="GeneID:72399"
                     /db_xref="MGI:MGI:1919649"
     exon            1..286
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..1980
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     impedes mitogenic signal propagation; RING-type E3
                     ubiquitin transferase BRAP2"
                     /codon_start=1
                     /product="BRCA1-associated protein isoform 1"
                     /protein_id="NP_082503.2"
                     /db_xref="CCDS:CCDS19640.1"
                     /db_xref="GeneID:72399"
                     /db_xref="MGI:MGI:1919649"
                     /translation="MSVSLVVIRLELAGHSPVPTDFGFSAAAGEMSDEEIKKKTLASA
                     VACLEGKSAGEKAAIIHQHLGRREMTDVIIETMKARADEVRDTVEEKKPSAAPVSAQR
                     SREQSESVNTAPESPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSA
                     MLCVLTVPATMTSHDLMKFVAPFNDVIEQMKIIRDSTPNQYMVLIKFSAQADADSFYM
                     ACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNG
                     ILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHI
                     GCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEG
                     DTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEK
                     CDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQELNKCLRANQLVLQNQLK
                     EEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQINIAMA
                     SAPNPPSSGAGGKLQSRKGRSKRGK"
     exon            287..448
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            449..644
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            645..834
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            835..948
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1097
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1173
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1312
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1422
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1512
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1513..1616
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..3737
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3710..3715
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /note="hexamer: AATAAA"
     polyA_site      3736
                     /gene="Brap"
                     /gene_synonym="3010002G07Rik; BRAP2; IMP"
                     /note="major polyA site"
ORIGIN      
        1 gggtcagaga cggcggccga caggcggaag cggaggttgt tgtggctcgg caggctgcgc
       61 gcgacggggg ctgccgctgc tgaggcggcg gccgcggctt ctgaggcggt ggcggcgctg
      121 ccgaccgcgg gccgctcggc ctctgggctc gggccgcagc cttccgtcag cccaccgggg
      181 cccacgccga gcagcgcctg tcctatgagt gtgtcactgg tggtcatccg actggagctc
      241 gcgggtcact cgccggtccc caccgacttc ggcttcagcg ccgccgctgg agagatgtct
      301 gatgaggaga tcaaaaagaa gacattggct tccgctgtcg cctgtctaga agggaagtca
      361 gccggggaga aagcagccat catccaccag caccttggcc gtcgggaaat gacggatgtg
      421 attattgaga ccatgaaggc cagagcagat gaagtgagag acacagtgga agaaaagaag
      481 ccttcagcag cccctgtctc tgcacagaga agtagagaac agagtgagtc tgtgaacaca
      541 gccccagagt ctccatccaa acagctccca gaccagattt ccttcttcag tggcaaccct
      601 tctgttgaaa tagtccacgg cattatgcac ctgtacaaga caaataagat gacctcccta
      661 aaagaagacg ttcggcgcag tgccatgctg tgtgtcctca ccgtccctgc caccatgacc
      721 agtcatgacc ttatgaagtt tgttgcccca ttcaatgatg taattgaaca aatgaaaatc
      781 atcagggact ctactccgaa tcagtacatg gtactgatca agttcagtgc gcaggctgat
      841 gcagacagtt tctacatggc gtgcaatggc cgccagttca actcaatcga agatgacgtc
      901 tgccagctgg tctatgtgga aagggctgaa gtgctgaaat ctgaagatgg cgccagcctc
      961 cccgtgatgg acctgacgga gctgcccaag tgcactgtgt gtctggagcg gatggacgag
     1021 tctgtgaatg gcatcctcac caccctctgc aaccacagct tccatagtca gtgtctgcag
     1081 cggtgggatg acaccacgtg tcctgtgtgc cgatactgtc aaacgccaga gccagtggaa
     1141 gaaaacaaat gttttgagtg tggtgtccag gaaaacctct ggatttgttt aatatgcggc
     1201 cacataggct gtgggcggta cgtgagtcgg catgcttaca agcactttga ggagacccag
     1261 cacacatacg ccatgcagct caccaaccat cgagtctggg actatgctgg agataattat
     1321 gtccatcgac tggttgcaag caagacggat ggaaagatcg ttcagtacga gtgtgagggc
     1381 gacacctgcc aggaagagaa gatagatgcc ttacagttag agtactcgta cctgttgaca
     1441 agccagctgg aatcgcagcg gatatactgg gagaacaaaa tcgtgcgcat agagaaggac
     1501 acggcagagg agattaacaa catgaagacc aagtttaaag agaccatcga gaagtgtgac
     1561 agcctggagc tcaggctcag tgacctcctg aaggagaagc agtctgtgga aaggaagtgt
     1621 acccagctga acaccagagt ggccaagctc agcacggagc tgcaggagga gcaggagctg
     1681 aacaagtgtc tgcgcgccaa ccagctggtg ctgcagaacc agctcaagga ggaggagaag
     1741 ctgctgaagg agacctgtgc ccagaaagac ctgcagatca ccgagatcca ggagcagctg
     1801 cgcgatgtca tgttctacct ggagacacag cagcagatca gccacctgcc tgcggagacg
     1861 aggcaggaga tccaggaagg ccagatcaac atcgccatgg cctcagcgcc caacccaccc
     1921 tcttccgggg ccggtgggaa gctgcagtcc agaaagggcc gcagcaagag gggcaagtga
     1981 ccatcagaga cttgtccctg agacttcctg ggcaccacag ggtctgctgg gacctttgct
     2041 gagcggaagt gtgggcccta ataagtacgt ctgaggacga actgtggtca tgtggctcct
     2101 tcatttgttg agtgtagtgt gaggagacta aaagaggggg aagggttggc agcacttccc
     2161 aagggtgagt cacctcatgg ccattctgcc ttccagctgg gtagccccca ggcttgtgcg
     2221 gttttccatt gtatttattc agttggcgca cttgcagttg ggttccaggg taaatctatg
     2281 acatgacgta accttcctag cactaaagga attccagcat taactagcac taaagcacag
     2341 tgcctcccag aggcccagct gccttcagga gaggagccca ctgcccactg cagaaggtct
     2401 ttcctgccat ccctccctcc cttctcctgc agagtagaag agagagctgc ttgcatgtca
     2461 tagagaaagg gctaggttgt aaaccaagca caggcttgtc ttcagcagcc tggcttgggg
     2521 caaatggagt atacccagtt tagggaagac agtgacagct ctctttattt ctgatgactc
     2581 catccgaaat gtgcctgtca ataagaaaag ccttcctggc tccttctcag ctcaggtaac
     2641 tgagagtgtc ctccatccag caggattcaa ggggcaagcc tagctagcca tcgagaggcc
     2701 tctgacaagc tgactgactt gtgttgctgg ggtcagtgga tactgactgt ttacaggcag
     2761 ggccgcatgt tcaggtcgca gctagaggga gcttgggcag atttcagtta ctcttttatg
     2821 atcgtttcaa aggctgcttc tccagaaact ggcttcagca gagcctcttc caaacagcca
     2881 tacaatattt aatatgacca acagaggggc tgtgtgcctc tctttagagc caggaacatc
     2941 aggtaacttg taacagatta attgaacaga cttgagggga caccatgttg ggatttagct
     3001 tctaaaagag cctgggcagt tgacagctgt gtgtagtaag gacagtttaa ttttttgctt
     3061 tatcatcagc caaaggttta aaatgtcaca ctttagggac tttttttttt ttttttccga
     3121 ctgactggac ctcgtccttt gaccggaatg tctgacatgt caccacgttg cctgtgttgc
     3181 tgatcaccgt ccagttcagt ggaatgtgct gtagcatttc ctaaaacccc acctgtgact
     3241 gcagagtgag agtgctcaga ctcggacctc tgtagccggc tcttcctcag ggcaggccag
     3301 cactgcagat cctccggccc gagccacaca ggcggctggg gctggggctg gggctggggc
     3361 agagccgcct cttccagagc ttctatttta tctagaacgc gttacacact gttacagtct
     3421 taccggctca gccctgcttg tcttgcacat ctgaccttgc agaagctggg gtgggtggta
     3481 gcatgctaat taagagaatc agaagtagtt tccagagctg gctccccttt tcatcacaac
     3541 ccacccagca gccctgctgc ccagaggaga gtagcagtgc ttcaccacac aggttggggg
     3601 aaatgcctgg gaaggctgtg gccagtggaa gcagccgacg ggcagaggtt tccaaactac
     3661 ttgaaggtct tttaaaaatg ttccaggaaa acaaaaatca agatgtgtaa ataaaatgtt
     3721 attttaaaag gtcaaag
//
