LOCUS       NM_001145552            2189 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus BLOC-1 related complex subunit 8 (Borcs8), transcript
            variant 1, mRNA.
ACCESSION   NM_001145552
VERSION     NM_001145552.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Park,H., Miyazaki,H., Yamanaka,T. and Nukina,N.
  TITLE     Non-coding RNA Neat1 and Abhd11os expressions are dysregulated in
            medium spiny neurons of Huntington disease model mice
  JOURNAL   Neurosci Res 147, 58-63 (2019)
   PUBMED   30391555
REFERENCE   3  (bases 1 to 2189)
  AUTHORS   Yordanov,T.E., Hipolito,V.E.B., Liebscher,G., Vogel,G.F.,
            Stasyk,T., Herrmann,C., Geley,S., Teis,D., Botelho,R.J., Hess,M.W.
            and Huber,L.A.
  TITLE     Biogenesis of lysosome-related organelles complex-1 (BORC)
            regulates late endosomal/lysosomal size through PIKfyve-dependent
            phosphatidylinositol-3,5-bisphosphate
  JOURNAL   Traffic 20 (9), 674-696 (2019)
   PUBMED   31314175
REFERENCE   4  (bases 1 to 2189)
  AUTHORS   Timmermans,S. and Libert,C.
  TITLE     Overview of inactivating mutations in the protein-coding genome of
            the mouse reference strain C57BL/6J
  JOURNAL   JCI Insight 3 (13), 121758 (2018)
   PUBMED   29997285
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2189)
  AUTHORS   Pu,J., Schindler,C., Jia,R., Jarnik,M., Backlund,P. and
            Bonifacino,J.S.
  TITLE     BORC, a multisubunit complex that regulates lysosome positioning
  JOURNAL   Dev Cell 33 (2), 176-188 (2015)
   PUBMED   25898167
REFERENCE   6  (bases 1 to 2189)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 2189)
  AUTHORS   VanBuren,V., Piao,Y., Dudekula,D.B., Qian,Y., Carter,M.G.,
            Martin,P.R., Stagg,C.A., Bassey,U.C., Aiba,K., Hamatani,T.,
            Kargul,G.J., Luo,A.G., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Assembly, verification, and initial annotation of the NIA mouse
            7.4K cDNA clone set
  JOURNAL   Genome Res 12 (12), 1999-2003 (2002)
   PUBMED   12466305
REFERENCE   8  (bases 1 to 2189)
  AUTHORS   Hidaka,K., Morisaki,T., Byun,S.H., Hashido,K., Toyama,K. and
            Mukai,T.
  TITLE     The MEF2B homologue differentially expressed in mouse embryonal
            carcinoma cells
  JOURNAL   Biochem Biophys Res Commun 213 (2), 555-560 (1995)
   PUBMED   7646512
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BE626539.1, AK009829.1, AC124327.9, CX218046.1, AI627072.1,
            AI153493.1, BE623475.1 and AI480554.1.
            
            On Jun 11, 2015 this sequence version replaced NM_001145552.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189670.138457.1,
                                           SRR5189676.102034.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-228               BE626539.1         1-228
            229-727             AK009829.1         4-502
            728-728             AC124327.9         6893-6893
            729-810             AK009829.1         504-585
            811-863             CX218046.1         617-669
            864-1131            AC124327.9         7029-7296
            1132-1509           AI627072.1         15-392
            1510-1529           AI153493.1         35-54
            1530-1530           AC124327.9         7695-7695
            1531-1605           AI153493.1         55-129
            1606-2099           BE623475.1         1-494
            2100-2155           AI480554.1         35-90               c
            2156-2156           AC124327.9         8321-8321
            2157-2189           AI480554.1         1-33                c
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.15 cM"
     gene            1..2189
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /note="BLOC-1 related complex subunit 8"
                     /db_xref="GeneID:72368"
                     /db_xref="MGI:MGI:1919618"
     exon            1..274
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /inference="alignment:Splign:2.1.0"
     exon            275..387
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /inference="alignment:Splign:2.1.0"
     exon            388..452
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /inference="alignment:Splign:2.1.0"
     exon            453..563
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /inference="alignment:Splign:2.1.0"
     exon            564..643
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /inference="alignment:Splign:2.1.0"
     exon            644..2189
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      795..800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /note="hexamer: AATACA"
     polyA_site      815
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /note="major polyA site"
     regulatory      2146..2151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
                     /note="hexamer: ATTAAA"
     polyA_site      2189
                     /gene="Borcs8"
                     /gene_synonym="2310045N01Rik; Mef2bnb"
ORIGIN      
        1 attatctgta cgtgtagcag ggagtccttg acgaggcata ctataacatc aagaaatctc
       61 tatcctcccg acacgctcgc ataggactgc ctccttccgt ctatctcgtg tctgaataga
      121 agttacctcg taagaggagc cagcgcacag gacgccagaa cccatacctg aacgtttaaa
      181 cgtctcaacc tcccgacttc cgcttccggt ttctgggctc tgctggctgc cttcactatg
      241 gaagaacctg agatgcagct gaagggaaag aaagtcacag acaagttcac cgagagtgtc
      301 tatgtccttg ccaacgagcc gtcagtggcc ctgtaccggc tacaggaaca tgtgcgccgt
      361 tcactgccag agctggccca gcacaaggct gacatgcaga gatgggaaga gcagagccag
      421 ggcgccatat acaccgtgga gtacgcctgc agtgctgtga agagcttggt ggacagcagc
      481 gtgtacttcc gtagtgtgga aggcctgctc aaacaggcca tcagtatccg ggaccacatg
      541 aacaccagtg cccaaggcca cagccaggag aaactgtccc ctcctccttc cctggcctga
      601 ccctgaccct ggaagactca gggctacctg cttccccacc caggcctgga taccacattg
      661 aagactcacc aagaactgaa gacctagcaa gtggccagtg cctgctgcct actgattctg
      721 ccataattgt ctaccttggt cataaattag ggtcactctt ccaggagcat tcacataaca
      781 gtctttgtgc actgaataca ctgactgtcc agaaaccgtc tctttccatg ctggggtcac
      841 ataacctaca tcatgtcaca tagctgaagg cccgccctgc catgtgtgat gcaggactag
      901 gtgcttcccc tctcatagga gcccctggaa aaacagcacc cacctccatt cagggcaagg
      961 cctgagaccc tggcgtggcc actagcttcc atcttgtaga ccgtggtggg tcagggtgct
     1021 tacttggcct acactgggtt ctggcttgtg tccccagaat aacatacaca cacaccacaa
     1081 actctaacaa ccaaaagttg ccaaagtcat tggagcagtt gactggagga gagcagtttt
     1141 aggtctgatt aggaacttag ctgaaacagg gtggtggacc tcatggccta catggcctcc
     1201 aaagagtaag aaaccctgaa ccacccgccg cagcaaggac actgagagca agagagaggc
     1261 tctcggttgc tgaggagtcc ctgtcccaac ttggccccca acactgagca tctccttcac
     1321 agtttctatc ccagacccca taataacagg aggagccgag ggagccctcc ctactctctt
     1381 gaataccatc aataaagttc actgcacccc tcccccccaa aagacttcag ctgagcccat
     1441 gtacaggaag tgtttgacaa gcccagctgt gtgctagata gaagccctgt gccagttaca
     1501 catgctagga tctggggaga tggcttagcc agcaaaatgc ttgcctcaag tcggggacta
     1561 gagtctgacc atcagaccca cattaaaaaa gtcaggcgta gtggtcaaag cttgtagccc
     1621 agcatccaca caaatatatg tctgtagata catacataag aataagtaat agatgtgaaa
     1681 agtaccccct gggtccagcc acggaagcaa cagggtgctc agggcattgg ggaacagtcc
     1741 tggtgtgcca cagcccctgg gtacccttct cagctggcag ctggcacctt tcacaggttt
     1801 cagaacagtc tatctcccac ctcagcacct gttgtccaag cctcccaggg ccatggctaa
     1861 tttacatttc atttgtatcc ccccgccccc cacaagaaca gagcattggt ctaggtgcac
     1921 agtcctgcct acttacacaa ccagccagag gctcctggtc cttcaggagg aactgagtca
     1981 ctcagcaagg ccttatatct gcccacaacc aaggctcaca gccttccttc cccatgtaac
     2041 tctggagcca ggtggtcact gttctgtgct gtggggcatc cagaaggaac ctggcaccca
     2101 cccactgact aggataactg gaaaggagtc tgacctgaat gacgcattaa actcctgcag
     2161 atctgtgtca gggcaaaaag ggaacgaaa
//
