LOCUS       NM_175277               2282 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus bolA family member 3 (Bola3), transcript variant 1,
            mRNA.
ACCESSION   NM_175277
VERSION     NM_175277.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Tajima,K., Ikeda,K., Chang,H.Y., Chang,C.H., Yoneshiro,T.,
            Oguri,Y., Jun,H., Wu,J., Ishihama,Y. and Kajimura,S.
  TITLE     Mitochondrial lipoylation integrates age-associated decline in
            brown fat thermogenesis
  JOURNAL   Nat Metab 1 (9), 886-898 (2019)
   PUBMED   32313871
REFERENCE   3  (bases 1 to 2282)
  AUTHORS   Yu,Q., Tai,Y.Y., Tang,Y., Zhao,J., Negi,V., Culley,M.K., Pilli,J.,
            Sun,W., Brugger,K., Mayr,J., Saggar,R., Saggar,R., Wallace,W.D.,
            Ross,D.J., Waxman,A.B., Wendell,S.G., Mullett,S.J., Sembrat,J.,
            Rojas,M., Khan,O.F., Dahlman,J.E., Sugahara,M., Kagiyama,N.,
            Satoh,T., Zhang,M., Feng,N., Gorcsan,J. 3rd, Vargas,S.O.,
            Haley,K.J., Kumar,R., Graham,B.B., Langer,R., Anderson,D.G.,
            Wang,B., Shiva,S., Bertero,T. and Chan,S.Y.
  TITLE     BOLA (BolA Family Member 3) Deficiency Controls Endothelial
            Metabolism and Glycine Homeostasis in Pulmonary Hypertension
  JOURNAL   Circulation 139 (19), 2238-2255 (2019)
   PUBMED   30759996
  REMARK    GeneRIF: this study shows that in vivo, pharmacological knockdown
            of endothelial BOLA3 and targeted overexpression of BOLA3 promotes
            histological and hemodynamic manifestations of pulmonary
            hypertension
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159713.6.
            
            On May 29, 2018 this sequence version replaced NM_175277.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK131950.1, SRR7345562.1957527.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-79                AC159713.6         165009-165087
            80-203              AC159713.6         166799-166922
            204-292             AC159713.6         168575-168663
            293-2282            AC159713.6         173680-175669
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 35.94 cM"
     gene            1..2282
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /note="bolA family member 3"
                     /db_xref="GeneID:78653"
                     /db_xref="MGI:MGI:1925903"
     exon            1..79
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..358
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     bolA-like protein 3; bolA-like 3"
                     /codon_start=1
                     /product="bolA-like protein 3 isoform 1"
                     /protein_id="NP_780486.1"
                     /db_xref="CCDS:CCDS20278.1"
                     /db_xref="GeneID:78653"
                     /db_xref="MGI:MGI:1925903"
                     /translation="MAAWGPAAAAPLLRGSRGLPLWHCAQRMFASQTEGELKVTQVLK
                     EKFPRATAIQVTDISGGCGAMYEIKIESEEFKEKRTVQQHQMVNQALKEEIKGMHGLR
                     IFTSVPKC"
     exon            80..203
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            204..292
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            293..2282
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      512..517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      542
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /note="major polyA site"
     regulatory      2256..2261
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
                     /note="hexamer: AAGAAA"
     polyA_site      2282
                     /gene="Bola3"
                     /gene_synonym="1810056O20Rik"
ORIGIN      
        1 gtgacctcgg gctgcgtcgc cgggcatggc ggcatggggc ccggcggccg ctgcgcctct
       61 gctgcgcggg agccgcgggc tccctctgtg gcactgtgcg cagcgaatgt ttgcctctca
      121 gacggagggg gaactcaaag tgacccaggt tctcaaagaa aagtttcctc gagccacagc
      181 tattcaagtc acagacatct caggaggctg cggggccatg tatgaaatta aaatcgaatc
      241 ggaagaattt aaagagaaga ggactgtcca gcagcaccag atggtcaatc aggcactaaa
      301 agaagagatc aagggcatgc acggccttcg gatcttcacc tctgtcccca agtgctgaca
      361 gctgactgac tgctcacgct taaaacctgg agtgcacttc gctcacagca gtattttaga
      421 aaatccctgc ccacctacaa aaatatctat tttttgttca tagacatttc tatattataa
      481 ttatgggaga tacattgtct atttagagtt aattaaagtt cacaggtgcc tcctgctcag
      541 aacagatgtg ccatgcttca ggtgccccct ttcagaagca gtgacttttg ctcacactgt
      601 tggagtctct gtacagttca tcctactcct ggacacagag tgaaggccag agttttggtt
      661 ggttttgctt tttgaaacag gatctcattg tgtagccctg gctggcctgg aactatttag
      721 accaggctaa cttgaacttc taggtctcct gcttggatga aaggcgtgtg tcaccatgcc
      781 tgatgccagg gttttggttc tatgaagttt tagggactct ctcctcccat acttctatta
      841 gaatgacgaa cccccagttt ctcatctgca tctggtgggg tgagtttgcc agcagctgca
      901 cttttaaagc tgaccttgca cacactcacc ctgggctctg ggggtgactc tgggaagctg
      961 ggacatctgt ctggatgtca gggtcaccaa gtctggtgtt ctgggtagac attctagtgt
     1021 ctgatgctca gtcagtgtct ccgcatccac gacagtaggc agagggtgca ggctgacttt
     1081 cacccagctt cagggcctgc cttcttgtgt ttccaggagc tggctcacca gtgttcccct
     1141 aaatccagtg gccaaatacc tcgaatgtgg cttctgcctt cccggccatc cccacatccc
     1201 tcacatcctc actgaatagt tttttgtttt ttaagacagg gtctttctac agagttctag
     1261 ctgttctgga actcactgtg gacacaccag gatggcctcg aactcagaga tccacctgct
     1321 tctgcctctg gagtgatggg attaaaacct gcaccacacc tggcgacagc tttttttaaa
     1381 aatctgtgtg cgcatgtgca tgaggttgtg cttattgtac gtgtgctaca gtgcacactc
     1441 gggaggcaga gaaaacatgc agatctgagt cctggggtct gaactcaggc ttggcagtga
     1501 gcacttttac cagctgagtc tataggtgtt ctgggcatct taagatgtct taggtctgcg
     1561 tgggaagtca cttaatcttc ataactttag accaagtgtg aaaacccacc agggtcccag
     1621 ctttggaggg ctctgctgtg gtcttcttcc agcacacccc tccctgcgag tgggcataaa
     1681 gtgtctgtca tggtgggctc atgttagtct gagttagtca aaagcccgtg ccaggatacg
     1741 ggtgggctaa cagtcacgag gaagagcggg cttgtggacg cttaggggta gaatccagat
     1801 ttggtatgag gatgccacta ggaaggagga gggaggcctg gcagagtggc tttgattttg
     1861 tggcacttac tgcttcagct ctgacaaatt gccatggagc accatgcagc cacccagaag
     1921 tcaccagtga ggccagagca agtcatggct ttgtagagcc ctgacactgg tagtcacaca
     1981 aaggcaggtg gctgcagcgg gactcaatgc ctggacactg caactcaatg ccatgagcta
     2041 taaagctcat gtttgagagc cacgtgactg agtcagtcat aggaaaatta ctggaccagg
     2101 aggtggccac aaagtctgat gatctgagac ctgagctggg gtcctacagc ctacatggga
     2161 ggaggagagg acccattccc gccggtgggc tgccaacctt tacatgtgtg ccagggcggg
     2221 catctctccc agctgtcatg tacccaatat aagaaaagaa aaaaaaagtt tcaaaacacc
     2281 ca
//
