LOCUS       NM_009753               9667 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus BICD cargo adaptor 1 (Bicd1), transcript variant 2,
            mRNA.
ACCESSION   NM_009753
VERSION     NM_009753.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9667)
  AUTHORS   Budzinska,M.I., Villarroel-Campos,D., Golding,M., Weston,A.,
            Collinson,L., Snijders,A.P. and Schiavo,G.
  TITLE     PTPN23 binds the dynein adaptor BICD1 and is required for endocytic
            sorting of neurotrophin receptors
  JOURNAL   J Cell Sci 133 (6) (2020)
   PUBMED   32079660
  REMARK    GeneRIF: PTPN23 binds the dynein adaptor BICD1 and is required for
            endocytic sorting of neurotrophin receptors.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9667)
  AUTHORS   Terawaki,S., Yoshikane,A., Higuchi,Y. and Wakamatsu,K.
  TITLE     Structural basis for cargo binding and autoinhibition of
            Bicaudal-D1 by a parallel coiled-coil with homotypic registry
  JOURNAL   Biochem Biophys Res Commun 460 (2), 451-456 (2015)
   PUBMED   25796327
  REMARK    GeneRIF: findings suggest a molecular basis for cargo recognition
            and autoinhibition of BICD proteins during dynein-dependent
            intracellular retrograde transport
REFERENCE   5  (bases 1 to 9667)
  AUTHORS   Terenzio,M., Golding,M., Russell,M.R., Wicher,K.B., Rosewell,I.,
            Spencer-Dene,B., Ish-Horowicz,D. and Schiavo,G.
  TITLE     Bicaudal-D1 regulates the intracellular sorting and signalling of
            neurotrophin receptors
  JOURNAL   EMBO J 33 (14), 1582-1598 (2014)
   PUBMED   24920579
  REMARK    GeneRIF: BICD1 regulates neurotrophin signalling by modulating the
            endosomal sorting of internalised ligand-activated receptors.
REFERENCE   6  (bases 1 to 9667)
  AUTHORS   Garcia-Ortiz,J.E., Pelosi,E., Omari,S., Nedorezov,T., Piao,Y.,
            Karmazin,J., Uda,M., Cao,A., Cole,S.W., Forabosco,A.,
            Schlessinger,D. and Ottolenghi,C.
  TITLE     Foxl2 functions in sex determination and histogenesis throughout
            mouse ovary development
  JOURNAL   BMC Dev Biol 9, 36 (2009)
   PUBMED   19538736
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9667)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 9667)
  AUTHORS   Matanis,T., Akhmanova,A., Wulf,P., Del Nery,E., Weide,T.,
            Stepanova,T., Galjart,N., Grosveld,F., Goud,B., De Zeeuw,C.I.,
            Barnekow,A. and Hoogenraad,C.C.
  TITLE     Bicaudal-D regulates COPI-independent Golgi-ER transport by
            recruiting the dynein-dynactin motor complex
  JOURNAL   Nat Cell Biol 4 (12), 986-992 (2002)
   PUBMED   12447383
  REMARK    Erratum:[Nat Cell Biol. 2003 Jan;5(1):84.]
REFERENCE   9  (bases 1 to 9667)
  AUTHORS   Baens,M. and Marynen,P.
  TITLE     A human homologue (BICD1) of the Drosophila bicaudal-D gene
  JOURNAL   Genomics 45 (3), 601-606 (1997)
   PUBMED   9367685
REFERENCE   10 (bases 1 to 9667)
  AUTHORS   Baens,M., Aerssens,J., van Zand,K., Van den Berghe,H. and
            Marynen,P.
  TITLE     Isolation and regional assignment of human chromosome 12p cDNAs
  JOURNAL   Genomics 29 (1), 44-52 (1995)
   PUBMED   8530100
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK051379.1, AK043650.1, AK082523.1 and AC164090.3.
            
            On Oct 18, 2013 this sequence version replaced NM_009753.3.
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051379.1, AK045975.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-90                AK051379.1         2-91
            91-1175             AK043650.1         2-1086
            1176-3521           AK082523.1         1105-3450
            3522-9667           AC164090.3         1844-7989           c
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 78.94 cM"
     gene            1..9667
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="BICD cargo adaptor 1"
                     /db_xref="GeneID:12121"
                     /db_xref="MGI:MGI:1101760"
     exon            1..658
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     CDS             446..2953
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein bicaudal D homolog 1"
                     /codon_start=1
                     /product="protein bicaudal D homolog 1 isoform 2"
                     /protein_id="NP_033883.2"
                     /db_xref="CCDS:CCDS39723.1"
                     /db_xref="GeneID:12121"
                     /db_xref="MGI:MGI:1101760"
                     /translation="MAAEEALKTVDQYKTEIERLTKELTETTHEKIQAAEYGLVVLEE
                     KLTLKQQYDELEAEYDGLKQELEQLREAFGQSFSIHRKVAEDGETREETLLQESASKE
                     AYYLNKILEMQNELKQSRAVVTNVQAENERLSAVVQELKENNEMVELQRIRMKDEIRE
                     YKFREARLLQDYTELEEENITLQKLVSTLKQNQVEYEGLKHEIKRFEEETVLLNSQLE
                     DAIRLKEIAEHQLEEALETLKNEREQKNNLRKELSQYINLSDSHISISVDGLKFAEDG
                     SEPNNDDKMNGHIHGPLGKLNGDYRTPTTRKGESLHPVSDLFSELNISEIQKLKQQLI
                     QVEREKAILLANLQESQTQLEHTKGALTEQHERVHRLTEHVNAMRGLQNSKEIKAELD
                     CEKGRNSAEEAHDYEVDINGLEILECKYRVAVTEVIDLKAEIKALKEKYNKSVENYTE
                     EKTKYESKIQMYDEQVTNLEKTSKESGEKMAHMEKELQKMTGIANENHNTLNTAQDEL
                     VTFSEELAQLYHHVCLCNNETPNRVMLDYYRQSRVTRSGSLKGPDDPRGLLSPRLSRR
                     GVSSPVESRTSSEPVSKENTETSKEPSPTKTPTISPVITAPPSSPVLDTSDIRKEPMN
                     IYNLNAIIRDQIKHLQKAVDRSLQLSRQRAAARELAPMIDKDKEALMEEILKLKSLLS
                     TKREQIATLRAVLKANKQTAEVALANLKNKYENEKAMVTETMTKLRNELKALKEDAAT
                     FSSLRAMFATRCDEYVTQLDEMQRQLAAAEDEKKTLNTLLRMAIQQKLALTQRLEDLE
                     FDHEQSRRSKGKLGKSKIGSPKIVSSLLPPYRHSAHN"
     exon            659..871
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1024
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1450
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..2545
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2697
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2905
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..2981
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     exon            2982..9667
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3222..3227
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="hexamer: AATAAA"
     polyA_site      3239
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="major polyA site"
     regulatory      7264..7269
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="hexamer: AATAAA"
     polyA_site      7287
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
     regulatory      8684..8689
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="hexamer: AATAAT"
     polyA_site      8721
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
     regulatory      9019..9024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="hexamer: AATAAA"
     polyA_site      9047
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="major polyA site"
     regulatory      9112..9117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="hexamer: AATAAA"
     polyA_site      9134
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
     regulatory      9631..9636
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
                     /note="hexamer: AATAAA"
     polyA_site      9667
                     /gene="Bicd1"
                     /gene_synonym="B830009D06Rik; bic-D 1"
ORIGIN      
        1 agttggagtc cgcatctgtg tgcactggct gccgcagggc ccagcaagca ggtggagctc
       61 agcctcccac ggagcattgg gcggtggacc gcagtgacgc ggcctgagac atggcggcca
      121 cgcgcctctg aggccgcaga accggagtct cttgctgcgc gagttgcccc ttggccatgc
      181 agcttggctg ctgactgccc cggcgcgtgg cccggaggat cccggctgcg gtcatcacct
      241 tgcagtctcc atgctgcagg gtatattcct cccatattcc atgtattcct cctcagtgct
      301 tccggccgtg tagccgcagc tgccggccgg gcctcagggc gctctaccta ttattcatca
      361 ggcacttttc ttttccattt ttattttcat tcctatttgc ttctcctttt cccttctgct
      421 ttgcgtctat ctccgccacc ccgctatggc cgcagaggag gcgctgaaga cggtagacca
      481 atataagact gaaatagaga gactgaccaa ggagctcaca gaaacgaccc atgagaagat
      541 ccaggctgca gagtatgggc tggtggtgct ggaggagaaa ctgactctca agcaacagta
      601 tgacgagctg gaggccgagt atgacggcct caaacaagag ctggagcagc tcagagaggc
      661 atttgggcag tccttctcta tccaccgtaa agtagctgag gatggagaga ctcgggagga
      721 aacacttctt caggagtctg catccaaaga agcttactat ctaaacaaga tcttggagat
      781 gcagaatgag ctgaagcaga gccgggctgt ggttacaaat gtccaggcag aaaatgagag
      841 gctctcggcc gttgtacagg aactgaaaga gaacaacgag atggtggagc tacagagaat
      901 acgaatgaaa gatgaaatcc gagaatataa attccgggag gcccgactcc ttcaggacta
      961 cacagaattg gaagaagaaa atatcacatt gcagaaacta gtgtccacat tgaagcagaa
     1021 ccaggttgaa tacgaaggct taaagcatga aattaagcga tttgaagaag aaacagtatt
     1081 actgaatagc caactagaag atgccattcg actgaaagag attgcagagc atcagttgga
     1141 agaagccctt gagacactga agaatgaaag agagcaaaag aacaacctga ggaaggagct
     1201 gtcccagtac atcaacctca gtgatagcca catcagcatc tcagtagatg ggctcaagtt
     1261 tgctgaggat ggaagtgagc caaataacga tgacaagatg aatggacata tccatgggcc
     1321 tcttgggaaa ctgaatggag actatcgtac tcccactacc aggaaaggag agtccctaca
     1381 ccctgtgtct gacttattca gtgagttgaa catttcagaa atacagaaat tgaagcagca
     1441 gcttattcag gtagagaggg aaaaagctat tcttctggcc aacctccagg agtcacaaac
     1501 ccagctagaa cataccaagg gggcactgac agagcagcat gagcgagtgc accggctcac
     1561 agagcatgtc aatgctatga ggggcttgca aaacagcaaa gagatcaagg ctgagctaga
     1621 ttgtgagaag ggccggaact cagcggagga agctcatgac tatgaggtgg acatcaatgg
     1681 cttagagatc cttgagtgca aatatagagt ggccgtcaca gaagtcattg atttgaaagc
     1741 agaaattaag gccttaaagg aaaaatataa taaatctgta gaaaattata cagaagaaaa
     1801 gaccaaatat gagagtaaga tccaaatgta tgatgaacaa gtgacaaacc ttgagaagac
     1861 atctaaggag agtggtgaga agatggccca catggagaag gagttgcaaa agatgactgg
     1921 catagccaac gaaaatcaca acaccctcaa tacagcccag gatgagttgg tgacatttag
     1981 tgaggaacta gcccagcttt accaccatgt gtgtctatgt aataatgaaa ctcccaatag
     2041 agtcatgttg gactactata gacaaagcag agttactcga agtggcagcc tcaaggggcc
     2101 tgatgatccc agagggcttt tgtcgccaag attatccagg aggggtgtgt catcccctgt
     2161 agaatcaagg acatcatctg aaccagtttc aaaggaaaat acagagacta gtaaagagcc
     2221 aagtccaact aagactccca caatctctcc tgttattact gccccaccat catctccagt
     2281 tttggatacc agtgatattc gcaaagagcc aatgaatatc tacaacctca atgccataat
     2341 ccgggaccaa atcaagcatc tgcagaaggc agtggacagg tccttacagc tgtctcgtca
     2401 aagagcagca gcccgagagt tggcccccat gattgataag gacaaggaag ccttaatgga
     2461 agagattctc aaactgaagt ccttgctgag caccaaacgg gagcagattg cgacactgag
     2521 ggcagtgttg aaagccaata agcagacagc tgaagtggct ttagctaacc tgaagaacaa
     2581 atatgaaaat gaaaaggcaa tggttactga aacaatgaca aaactgagga atgaactaaa
     2641 ggctttgaag gaagatgctg caaccttctc atccctgaga gcaatgtttg caacaagatg
     2701 cgatgaatat gtcacacagt tggatgagat gcagagacag ttagcagctg cagaagatga
     2761 gaagaagact ctaaacactt tattgcgaat ggctatccag caaaaactcg ccctgacaca
     2821 gaggctggag gacttagagt ttgaccatga gcagtcccga cgcagcaaag gcaaacttgg
     2881 aaagagcaag atcggcagcc ctaaaattgt cagcagcctg ctgcctccgt accgccacag
     2941 tgctcacaac tagctgggag gcaagactac ccaacggtca gtccggacat tgccctcagt
     3001 aaggaacagc cacattccag ctcacagtgt gcacctcttc attgtctcac caagcctcct
     3061 tacccctagt cttcatctct catggatgaa tatctggact gaaggttttt tagtcacagt
     3121 gagggcactg cttaagatct agcgggtgtt ttcttcttgg atgttagatg tatatgtgga
     3181 ttccagtcca tcatttttga agagtaaaga gagttcagaa gaataaaatg atgcatagac
     3241 cttcgtgtga tcaatttctg gtttctctga atcacagatg acctgcaatc aaatggcaac
     3301 agttcactta aaactgaaat gaaacatgta tttcagatag ttggcttcac ctccatagca
     3361 taaaaaagat ccaagacaat gtaaaataga tatgctgtaa aatcatcttt ccctatactc
     3421 caaagtcagc tatagatgtt gattctaata attttcattg atacttactt tatattttat
     3481 tttctgtatg gtttatgttt ataaagatta tttctgtaag cagaattcat ttatttttaa
     3541 agagtcatag tttacttagt tatttaaagt tttaatttag tttaaaatct gaaaccggac
     3601 agattctggt cctttttctt gcagaaaatt acaaatgagg tgcatcagaa tgttaacaat
     3661 aactgttttg ctgctacatg aatattcttt atgcagttat tttctaatca gtaactcatt
     3721 aattgtcagt tattttttta actaggtttc ttcactaaac atttattaag taaatctaag
     3781 aaagaggcaa agctatgatt cattgactta atcaagtttt gagggtatct ttaatttttt
     3841 aaaattgcag ttgagtagat agatatattg gtgctgctcc tgtgtgtgtg tgtgtgtgtg
     3901 tgtgtgtgtg tgtgtgtggt cgtattaaag aaggacaaat tacttgaagg aaagaaatgg
     3961 tgtgcttatt taatgaccac aaatttttta aacttttcca aatttagggt cttactatgg
     4021 gtttcccact aaaagggatc acaataaatg caaaaatata tacatatata actgtaaggt
     4081 agccttggct agttatttta tgcagaaagt gtatgttttg ttgtttgttt tctttttcac
     4141 aatgaatctt ccttgccaaa aaaaaaaaaa atagatagta aaccttagct cattgtctac
     4201 ttttaacaaa cactcaggtg cctacagtca tattatattg agataagaca tgttcctact
     4261 taatttacag attctgctag gtaattttat gactttattt aaggcaatag aatttcatag
     4321 aaaaatttag tttttgcatt attgagaaaa caaggacaac agctagttga gctgtaaggt
     4381 ctaacttgga ttcctcagtt ctcaagtgct gcccacataa ctgcgcctaa ccctgactcc
     4441 ttgtctttgt caggtagcct taacttacat aaaaccataa aggttttatt ttttcatgta
     4501 acagtatctt taacttttct tcaaaacaga actagtcctc actgttagca aaaagatgac
     4561 tagttttgta ggacaccaac aatggcgatt ttagagctac ccagcttgtg gagggaaaga
     4621 tgtgcatgtt tatggagtgg tgacctaagg tcagctgtca gccacaaagc acattacaga
     4681 aatatttttt gtttgttatg ttcctgttga gtaccagtca tagttaccaa agcaacccaa
     4741 agattttgtg ttctttatcg gattaataat agaccctatg gaacttctca tcagtcagaa
     4801 tccctacaaa gtcgtccctc ctgtattact tggttgttcc tatacatttt cctcatttat
     4861 tggtgttaag tctacctggt gccaatctta gtgagacagt acagattttt gtcagagttc
     4921 aattaaagac aacttcctct gttctttcta ttcttttccc cctttgctaa gcatgattcg
     4981 aaacccaagt gtcagaatta ctaagttaat tttgtatttg aggaaataag tacctcaaag
     5041 caagttgtgt ctttgggctg ggaactcaac agtggatctc ttctctgtgt aaccactgtt
     5101 gacatagctg cattgtgttt gtgaatagag ttgacttctt ccagtatgaa atttatcact
     5161 gtctgttata aacgatttta ttatagttgt ttatttgaat atagtggaaa tttttgtttt
     5221 gaaatattac ttctataaaa tattaatttt aacaacttta ataagaagat atagaaattt
     5281 tgaaacaaat ttctcatttt cagcacaaaa gtaggtaata attgctaagc agtaagagac
     5341 tatcatgtat ttatgtagta acatcattta gtgcagttta aactgagctg tatttatttt
     5401 tatttattta cggacccttt caatgaataa atgtagagtg ctgataaaaa cgagtaccta
     5461 taaagataat atcagaatgt aaaaagtgaa aactggtgaa acaaaaaacc catgaatctc
     5521 aataagagaa ttgaaggtgc atttgatatt aaaatataaa gtaaagaaat tctacaaatg
     5581 ctaattgttg tagacatttt gaacaaaggt gagaggtgat gtttaagatc agttatcaaa
     5641 gtaaccaact actgttataa aatttcatta cattgtagtg cgtgcatgta tgtgtgtgtg
     5701 ctcacccaca actactgtta tacatttcat tacattgtag agtgtgtgtg tgtgtgtgtg
     5761 tatacacgcg cacacacaca cacacacaca ctatccacag tatgtgtggg aaagtcagag
     5821 gaaaacctac agaagttgct tctctcccac aactgtatgg atcccaggga ttgaactaag
     5881 attatcaggc ctagtggaaa acatctttac ctgctgagcc atcttattgg ttcccacggt
     5941 tttgtttttg gttttttaat gtaagcacaa ttacaaagct gaatagatga attagtatgc
     6001 agaaatgctt tttcactaag gattttcatt aaagcataat agtaaaataa aaagaagcaa
     6061 taaattgctt ttggcctaag tgcttaggag catgaacaaa actttggatg tataaacctt
     6121 ttgcatatct aattctggat ttaaaagaag agagaggagg aaaaatgaat tttgaaaact
     6181 tctagagaat ataaatctat aaaggaatga agaaaagact tatttggaaa ttataattta
     6241 tatttgccta aaggagaaga ggtacaataa tgaaatccaa caaaaagaac ttaacaaatg
     6301 acagagagta gctacagagc ctatatgact aaatgagaag ggttaatatt acctatagga
     6361 aagagatatt ttaaagtgga aacttaatca ttcgatagct ataaagattt gtttttaaaa
     6421 actggaatga tactggtagt cttgtaagta tagacaattt acaataatat ataatcctag
     6481 gagtccacaa ttctatgatt cttttgtttt aaaatttaaa atatattgaa tttttactga
     6541 ttggtcatag aaaatgttta cttttaaact aggataacaa tcttaatttt ctaagatatt
     6601 tattattttt aaaaggattt ctttaaaggt atctgaagta tagcaactcc atttaaagaa
     6661 gcttcagtgt cagagtctaa aaaacaaata tacccaagaa aacttcataa atacagtgta
     6721 tgcaatctca tagaggagac aaatactttt taaattttat tcacacacaa agatcttctg
     6781 tctttaacat ttgctttatc tatgctgtag aacatgttct tatacagata agcccttctc
     6841 tattaggtaa gcatgaacaa ttggtctact gaactagcta agaatgtgga tgttccctca
     6901 tatactatat tgaataaaat cctctccagt aaccaagtac aggttacaaa taattatatg
     6961 aataaatcaa atatattttc tactctgata aatgtcaact tttattgtat aattcagaca
     7021 aaagatttaa tctttcttac aaagaaatca gtgggcagga gtagggagta actcagccag
     7081 gggtgtttgc tgaacctgtg tgagaggata gcacccactg cactgcaaaa caaacaacaa
     7141 aacaaacact gatatttccc tgaataaaat cctgctcatt caatccaagg atgtcacaaa
     7201 tgaattaata ttgtacatat aatgaaattt gtaaaaccca caatgtttaa aacttttgtt
     7261 tttaataaat cagagtgatt taaataacct tgcattaagg ctgcaaaatt taaataaatt
     7321 tcaaataaag ctgaaagcta tcgaagtcat gctagtcagc atggccagtg ccaaggttga
     7381 aaagtactta cttcaaaatc agtgtcctag ctcctgagtt accataccca ttttctgtaa
     7441 taataaccca agatgagttt tttgaaaata aatttatctt taaatgttat tttaaccacc
     7501 atagtcccta atatcttgta acctgttttt attttaatac ccaaaagatt tcaaataaga
     7561 tatcttgttt attactcaag atataaatta atgagataat tagtgttaaa atttaaaatg
     7621 ttcttaaatt tgggattttt cagaaatgac aatctgttta taaggagcaa aattaacctt
     7681 cattagtgta aagcaagagt ataagacgtt tgcatgaaat atttcacatc atgtaagctt
     7741 tcccatatcc ataacattga ctctatatag aaatcttact tgctgtgtga tctcgccatt
     7801 aggaatgaag gaggctgtta catgcactgg atctcctttc catactgaag cgcatggctt
     7861 tgttcctcaa atttattttt acatctctca gatggagtta tttaataatc tgccacaatt
     7921 gaactgaagc ttaaatgacc tgctgattcg gataatttct ttgattgctc tacatttgct
     7981 gagaacttat ttttatttta acttctgcac ataatcagtc tctgctcagt atacctctct
     8041 aatgagaccg ttgagaagta aatttgatgc ttcttagtgt ggatgtatgc ttattttttt
     8101 tcaaaaaaat aatttttttt tacattttat tttatttatt tattgttatt tttttagaca
     8161 gagtttctct gtatactcct ggctgtcctg aaactcactt agaacaggct ggcctcaaat
     8221 ttgcagagat ccatctgcct ttccaaaaaa gtctttatca gagaactatg aagttaaggg
     8281 aaataacact gttgtgttga caaatcttta tcttgtaagg gagaaacaga aggaatcatt
     8341 gggttcatag ctatgactat gtgtcatatc agaggaaata ctttttaatt ttcttgccac
     8401 aaaactttta agctcaatgg tttgactgtt agagtcaatt aacaattttt gaaaatattt
     8461 tatgactttt ttgtaatata ttcagtgtct ttatttgcat ggctatgcat atatgaagat
     8521 gtgactctga cgttgtgatt ctttcccatg ttaaaatttg tgttgtgtgt ggtcaaacct
     8581 aatttttaca ctgtatgatt atccttgtaa tgattcagtt attccagcct ttgttattat
     8641 gccttcgggg agtaaacaat gtgaagtgtt tctatctaat aataataata ccatgaaaac
     8701 atgactgaaa aactaattta agaaatgcct ttcttgctaa ttatttttga aatccaaaca
     8761 tcagcgacat tatcaaagac acgtgcattt aagtacaaaa agttcttata aatttattgc
     8821 ttgctgttct ttttactttt ttgtagataa tcccaagtga acctctcaag ggagaatcct
     8881 tatgtcatat gtttgtttct gtgcactcaa tatttgtgaa aggcacccca ctgctaagga
     8941 aataactaga tactaataat tattatcttg gttaatctta ccaattgacc atactgtatt
     9001 gcaaaacttt ttccagtaaa taaactgatt atcttttgtt tatttcatca atgttaaatt
     9061 tttattagtt tacaattttt ctgtttgcca tttttattta tacctacaag aaataaaaag
     9121 tataggaatc actatatgat tatatatttt tatccaatat aagaccttat ttgtcttaac
     9181 atttctgcat attttgtgat gtgtatcaac ttcattttcc attttccaaa ctcataatgt
     9241 ttccttaaag ggtattttgg caggaaaatg cacttagcta aattaaacta gaaatgtatt
     9301 aatacaggaa acactgtttt aatgaaagga aaatgacact ttattatgga gatggttctt
     9361 tttttctttt acataggaaa gagctaagcc agcaaactcg agggaggtca cttttactgg
     9421 acctacaata taaatcatgg tagatcagct gctctgagga tcatctgttt tgattctgtt
     9481 ttattttaca gtggtgcagg gttaaggagg aaacaggcaa gtatgggttt atgtgaagtg
     9541 ataatgaaca aaaatgaaac ccttataact gaccaaattt atgggcctgg ttaaagttgc
     9601 ctcttcttct ttggcacaga aaacattaaa aataaagtgt cttaaaaatc attccctttt
     9661 ctaaaaa
//
