LOCUS       NM_007542               2423 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus biglycan (Bgn), transcript variant 2, mRNA.
ACCESSION   NM_007542
VERSION     NM_007542.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Zhu,M., Wu,S.C.M., Tam,W.K., Wong,C.K., Liao,P., Cheah,K.S.,
            Chan,D., James,A.W. and Leung,V.Y.
  TITLE     Biglycan fragment modulates TGF-beta activity in intervertebral
            disc via an eIF6-coupled intracellular path
  JOURNAL   Sci Adv 11 (7), eadq8545 (2025)
   PUBMED   39951526
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2423)
  AUTHORS   Beach,Z.M., Nuss,C.A., Weiss,S.N. and Soslowsky,L.J.
  TITLE     Neonatal Achilles Tendon Microstructure is Negatively Impacted by
            Decorin and Biglycan Knockdown After Injury and During Development
  JOURNAL   Ann Biomed Eng 52 (3), 657-670 (2024)
   PUBMED   38079083
  REMARK    GeneRIF: Neonatal Achilles Tendon Microstructure is Negatively
            Impacted by Decorin and Biglycan Knockdown After Injury and During
            Development.
REFERENCE   4  (bases 1 to 2423)
  AUTHORS   Lin,Y., Yang,Q., Lin,X., Liu,X., Qian,Y., Xu,D., Cao,N., Han,X.,
            Zhu,Y., Hu,W., He,X., Yu,Z., Kong,X., Zhu,L., Zhong,Z., Liu,K.,
            Zhou,B., Wang,Y., Peng,J., Zhu,W. and Wang,J.
  TITLE     Extracellular Matrix Disorganization Caused by ADAMTS16 Deficiency
            Leads to Bicuspid Aortic Valve With Raphe Formation
  JOURNAL   Circulation 149 (8), 605-626 (2024)
   PUBMED   38018454
REFERENCE   5  (bases 1 to 2423)
  AUTHORS   Guo,P., Lim,R.C., Rajawasam,K., Trinh,T., Sun,H. and Zhang,H.
  TITLE     A methylation-phosphorylation switch controls EZH2 stability and
            hematopoiesis
  JOURNAL   Elife 13, e86168 (2024)
   PUBMED   38346162
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2423)
  AUTHORS   Bergsagel,P.L., Timblin,C.R., Kozak,C.A. and Kuehl,W.M.
  TITLE     Sequence and expression of murine cDNAs encoding Xlr3a and Xlr3b,
            defining a new X-linked lymphocyte-regulated Xlr gene subfamily
  JOURNAL   Gene 150 (2), 345-350 (1994)
   PUBMED   7821804
REFERENCE   7  (bases 1 to 2423)
  AUTHORS   Scholzen,T., Solursh,M., Suzuki,S., Reiter,R., Morgan,J.L.,
            Buchberg,A.M., Siracusa,L.D. and Iozzo,R.V.
  TITLE     The murine decorin. Complete cDNA cloning, genomic organization,
            chromosomal assignment, and expression during organogenesis and
            tissue differentiation
  JOURNAL   J Biol Chem 269 (45), 28270-28281 (1994)
   PUBMED   7961765
REFERENCE   8  (bases 1 to 2423)
  AUTHORS   Rau,W., Just,W., Vetter,U. and Vogel,W.
  TITLE     A dinucleotide repeat in the mouse biglycan gene (EST) on the X
            chromosome
  JOURNAL   Mamm Genome 5 (6), 395-396 (1994)
   PUBMED   8043960
REFERENCE   9  (bases 1 to 2423)
  AUTHORS   Chatterjee,A., Faust,C.J., Molinari-Storey,L., Kiochis,P.,
            Poustka,A. and Herman,G.E.
  TITLE     A 2.3-Mb yeast artificial chromosome contig spanning from Gabra3 to
            G6pd on the mouse X chromosome
  JOURNAL   Genomics 21 (1), 49-57 (1994)
   PUBMED   8088815
REFERENCE   10 (bases 1 to 2423)
  AUTHORS   Bianco,P., Fisher,L.W., Young,M.F., Termine,J.D. and Robey,P.G.
  TITLE     Expression and localization of the two small proteoglycans biglycan
            and decorin in developing human skeletal and non-skeletal tissues
  JOURNAL   J Histochem Cytochem 38 (11), 1549-1563 (1990)
   PUBMED   2212616
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL732461.23 and AK164867.1.
            
            On Oct 16, 2015 this sequence version replaced NM_007542.4.
            
            Summary: This gene encodes a small, leucine-rich repeat
            proteoglycan that plays important roles in bone mineralization and
            connective tissue metabolism. The encoded preproprotein undergoes
            post-translational processing during which chondroitin sulfate or
            dermatan sulfate chains are attached before incorporation into the
            extracellular matrix. Mice lacking the encoded protein exhibit
            reduced growth rate and acquire diminished bone mass progressively
            with age. [provided by RefSeq, Oct 2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019502.1, L20276.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AL732461.23        214244-214277
            35-1349             AK164867.1         2-1316
            1350-2423           AL732461.23        225506-226579
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 37.33 cM"
     gene            1..2423
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /note="biglycan"
                     /db_xref="GeneID:12111"
                     /db_xref="MGI:MGI:88158"
     exon            1..162
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /inference="alignment:Splign:2.1.0"
     exon            163..414
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..1283
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 2; bone/cartilage proteoglycan I"
                     /codon_start=1
                     /product="biglycan isoform 2 preproprotein"
                     /protein_id="NP_031568.2"
                     /db_xref="CCDS:CCDS30204.1"
                     /db_xref="GeneID:12111"
                     /db_xref="MGI:MGI:88158"
                     /translation="MCPLWLLTLLLALSQALPFEQKGFWDFTLDDGLLMMNDEEASGS
                     DTTSGVPDLDSVTPTFSAMCPFGCHCHLRVVQCSDLGLKTVPKEISPDTTLLDLQNND
                     ISELRKDDFKGLQHLYALVLVNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPS
                     SLVELRIHDNRIRKVPKGVFSGLRNMNCIEMGGNPLENSGFEPGAFDGLKLNYLRISE
                     AKLTGIPKDLPETLNELHLDHNKIQAIELEDLLRYSKLYRLGLGHNQIRMIENGSLSF
                     LPTLRELHLDNNKLSRVPAGLPDLKLLQVVYLHSNNITKVGINDFCPMGFGVKRAYYN
                     GISLFNNPVPYWEVQPATFRCVTDRLAIQFGNYKK"
     exon            415..527
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /inference="alignment:Splign:2.1.0"
     exon            528..741
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /inference="alignment:Splign:2.1.0"
     exon            742..852
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /inference="alignment:Splign:2.1.0"
     exon            853..946
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1085
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..2423
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2399..2404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /note="hexamer: ATTAAA"
     polyA_site      2423
                     /gene="Bgn"
                     /gene_synonym="BG; DSPG1; PG-S1; PGI; SLRR1A"
                     /note="major polyA site"
ORIGIN      
        1 agcctttagc ttccctccca ccgcctctgt ctccctctct ccacgaactg cccaggagcg
       61 agcagctgct cccggttggc cctgacggac agacaaaccg acagcctgac aacctagtcc
      121 accaactaag cagcctgcac ctggctgctt gtccctcccc aggaacattg accatgtgtc
      181 ccctgtggct actcaccttg ctgctggccc tgagccaggc cttgcccttt gagcagaagg
      241 gtttctggga cttcaccttg gatgatgggc tgctcatgat gaatgatgag gaggcttcag
      301 gttcagacac cacttcaggt gtccccgacc tggactctgt cacacctacc ttcagtgcca
      361 tgtgtccttt cggttgccac tgccacctgc gggttgttca gtgctctgac ttgggtctga
      421 agactgtgcc caaggagatc tcacctgaca ccacactgct agacctgcag aacaatgaca
      481 tttctgagct tcgcaaggat gacttcaaag gcctccagca cctctacgcc ctggtcttgg
      541 taaacaataa gatctccaag atccatgaga aggcctttag ccctctgcgg aagctgcaaa
      601 aactctacat ctccaagaac cacctggtgg agattcctcc caacctgccc agctccctgg
      661 tagaactacg aatccatgac aaccgtatcc gcaaagtgcc caagggcgtg ttcagcgggc
      721 tccggaacat gaactgcatt gagatgggcg ggaatcccct ggagaacagt ggctttgaac
      781 caggagcctt tgatggcctg aagctcaatt acctgcgcat ctcagaggcc aagctcactg
      841 gcatccccaa agatctccct gagaccctga acgaacttca cctggaccac aacaaaatcc
      901 aggctattga gttggaggac ctacttcgat actccaagct gtacaggttg ggcttaggtc
      961 acaatcagat tcggatgatt gagaatggga gcctgagttt tctgcctacc ctgagggaac
     1021 ttcacttgga caacaacaag ctgtcccggg tgcctgctgg cctcccagat ctcaagctcc
     1081 tccaggttgt ctatctgcac tccaacaaca tcaccaaggt gggcatcaat gacttctgtc
     1141 ctatgggctt cggagtcaag agggcctact ataatggcat cagcctcttc aacaaccctg
     1201 tgccctactg ggaagtgcag cctgccacct tccgctgcgt tactgaccgc ctggccatcc
     1261 aatttggaaa ttataagaag tagaggcagt ggttgccacc atggtggcct tggtgagagt
     1321 ctctgaggaa catagccaga tgaagaagca acacctttgt tccccaatat taactcactg
     1381 ccccaccaca gcttccccct gactcctaag catgcatata tgcacatggc ctggccctct
     1441 cacccattcc cctcaacctt tgaaatttaa cattcatcaa ccatgtccac tcagagactc
     1501 cctataaatc tttcttcttg ctcatcctga aactcagatg tttttggcaa gaggggctag
     1561 gaaagatgga tagagcacac tgccaccgcc attgttccat ccaggcatgt gttcctcctc
     1621 ttccttgctc atgtctgact tccagctctc ctgggctctg cttgctgccc ttatcctctg
     1681 gtgttctctc ttcaacaagt tcactacctg tcaatcccag ctacaacctg gctgtactaa
     1741 ctcctggatc tttccctctc tccaaccctg ttatgcttcc tgacactttt cttccttctg
     1801 gagttattga cctgtcccct tccatctctg gacctaggtc atatttctcc atctttgtct
     1861 ctttctgtat ctccttgcct atatctctgt ctgtctctat ttctgtctct ctgtctctgt
     1921 gtatctctct atctttgtat ctgtctctct ctgacaacac acacacacac acacacacac
     1981 acacacacac acacacacac acacacggat catctgcccc aggctgcttt ctgcttcaca
     2041 ggtctctagc cagtccctcc acaaacaaat atggggcaac tatcttcctg attgccctac
     2101 ccagaacttg accccccaac cctggaggaa gctggaaggt ggaggcccag aatcctgtcc
     2161 attttgtcca ggaaagggtt catactctgc tatcaagacg aggatcaagg agcttcctag
     2221 cccctggaga ggctcagcag gccatcagag ccgccagaac cagtttgcat tggcccctgc
     2281 tctctcccca agatggctag gtcccctccc tcacccctgg gtccctgatg tggtaggagg
     2341 tgatggtcag ttgcacccag caagagggag tgctgcttat gaggtcagtt gtctctcaat
     2401 taaagaaaca ctgtgcaata cga
//
