LOCUS       NM_001177552            2635 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus bifunctional apoptosis regulator (Bfar), transcript
            variant 2, mRNA.
ACCESSION   NM_001177552
VERSION     NM_001177552.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Das,A., Cheng,H., Wang,Y., Kinch,L.N., Liang,G., Hong,S.,
            Hobbs,H.H. and Cohen,J.C.
  TITLE     The ubiquitin E3 ligase BFAR promotes degradation of PNPLA3
  JOURNAL   Proc Natl Acad Sci U S A 121 (6), e2312291121 (2024)
   PUBMED   38294943
  REMARK    GeneRIF: The ubiquitin E3 ligase BFAR promotes degradation of
            PNPLA3.
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Ahuja,N., Hiltabidle,M.S., Rajasekhar,H., Voss,S., Lu,S.Z.,
            Barlow,H.R., Cowdin,M.A., Daniel,E., Vaddaraju,V., Anandakumar,T.,
            Black,E., Cleaver,O. and Maynard,C.
  TITLE     Endothelial Cyp26b1 restrains murine heart valve growth during
            development
  JOURNAL   Dev Biol 486, 81-95 (2022)
   PUBMED   35364055
REFERENCE   3  (bases 1 to 2635)
  AUTHORS   Mrowiec,K., Chadalski,M., Paszek,A., Vydra,N., Janus,P.,
            Pietrowska,M., Toma-Jonik,A., Gramatyka,M. and Widlak,W.
  TITLE     The periphilin 1-like BFAR isoform 3 is highly expressed in
            transcriptionally silent oocytes and involved in RNA metabolism
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1868 (10), 119086 (2021)
   PUBMED   34175335
  REMARK    GeneRIF: The periphilin 1-like BFAR isoform 3 is highly expressed
            in transcriptionally silent oocytes and involved in RNA metabolism.
REFERENCE   4  (bases 1 to 2635)
  AUTHORS   Pei,S., Huang,M., Huang,J., Zhu,X., Wang,H., Romano,S., Deng,X.,
            Wang,Y., Luo,Y., Hao,S., Xu,J., Yu,T., Zhu,Q., Yuan,J., Shen,K.,
            Liu,Z., Hu,G., Peng,C., Luo,Q., Wen,Z., Dai,D. and Xiao,Y.
  TITLE     BFAR coordinates TGFbeta signaling to modulate Th9-mediated cancer
            immunotherapy
  JOURNAL   J Exp Med 218 (7) (2021)
   PUBMED   33914044
REFERENCE   5  (bases 1 to 2635)
  AUTHORS   Wiese,C.B., Ireland,S., Fleming,N.L., Yu,J., Valerius,M.T.,
            Georgas,K., Chiu,H.S., Brennan,J., Armstrong,J., Little,M.H.,
            McMahon,A.P. and Southard-Smith,E.M.
  TITLE     A genome-wide screen to identify transcription factors expressed in
            pelvic Ganglia of the lower urinary tract
  JOURNAL   Front Neurosci 6, 130 (2012)
   PUBMED   22988430
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2635)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154607.2, AK028592.1 and AI255749.1.
            
            Transcript Variant: This variant (2) lacks two alternate exons,
            resulting in the loss of an in-frame segment in the central coding
            region, compared to variant 1. The encoded isoform (2) is shorter
            than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028592.1, SRR17253014.26193.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               AC154607.2         65821-65982
            163-1730            AK028592.1         2-1569
            1731-2514           AC154607.2         96678-97461
            2515-2635           AI255749.1         1-121               c
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 9.45 cM"
     gene            1..2635
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /note="bifunctional apoptosis regulator"
                     /db_xref="GeneID:67118"
                     /db_xref="MGI:MGI:1914368"
     exon            1..221
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /inference="alignment:Splign:2.1.0"
     exon            222..558
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /inference="alignment:Splign:2.1.0"
     CDS             296..1273
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="bifunctional apoptosis regulator isoform 2"
                     /protein_id="NP_001171023.1"
                     /db_xref="CCDS:CCDS49769.1"
                     /db_xref="GeneID:67118"
                     /db_xref="MGI:MGI:1914368"
                     /translation="MEEPQKNDLSMREQEEEHPVRSSGPQISVSEFSCHCCYDTLVNP
                     TTLNCGHSFCRHCLALWWMSSKKTECPECREKWEGFPKVNILLRLLLTLTEEEFSRAP
                     YTIENSSHRRVILTELERVRALGVKPPQNLWEYKAVNPGRSLFLLYALKSSPRLGLLY
                     LYLFDYTDCFLPFIHTICPLQENSSGEDIFTKLLDLREPTWKQWREFLVKYSFLPYQL
                     IAEFAWDWLEVHYWTSRFLIVNAVLLSVLELFSFWRIWSRSELKTVPQRMWSHFWKVS
                     TQGLFMAMFWPLIPQFVCNCLFYWALYFNPIINIDLVVKEVRRLETQVL"
     exon            559..703
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /inference="alignment:Splign:2.1.0"
     exon            704..877
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /inference="alignment:Splign:2.1.0"
     exon            878..1080
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..2628
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2600..2605
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /note="hexamer: AATAAA"
     polyA_site      2623
                     /gene="Bfar"
                     /gene_synonym="3010001A07Rik; 3110001I22Rik; Bar; Rnf47"
                     /note="major polyA site"
ORIGIN      
        1 gttggtgcgc atgcgcccgc atgcccctag agcagccgct ctaggcagcc ggaggtcgca
       61 cggggttgag agcataaaga ggtctctcct ctatggttgc gggagctgat cgtagtcggc
      121 ccctaggccc aggttatttc cggaagcgaa gccttgtcct ctacttccgc ggggtcctgg
      181 caggtggcgg tgacagagtg ggtaccagga ctagtggcga gactttatga tgcattgcag
      241 cagtgtcctg agtctgaaag tgtctatgtg tatggatccc agaatttgct gagagatgga
      301 ggagcctcag aaaaatgacc tgagcatgag agagcaggag gaagagcatc ctgtcagaag
      361 cagcggccct cagatttctg tgagcgagtt ctcctgccac tgctgctacg acaccctggt
      421 taaccccacc accttgaact gtggccacag cttctgccgg cactgcctgg ctttatggtg
      481 gatgtcttca aagaagacag agtgtccaga atgcagagaa aaatgggaag gttttcctaa
      541 agtcaacatt ctcctcaggt tgcttttaac tttgacagaa gaagagtttt ccagggcacc
      601 ttataccata gagaacagta gccacagaag ggtcatcctc acggagctgg agcgtgtgag
      661 agccctggga gtgaagccac cgcagaatct ctgggagtac aaggctgtca acccgggtag
      721 atccctattc ttgctctacg ctctcaagag ctcacccaga cttggcttgc tatacctgta
      781 cctgtttgac tacacagact gcttcctacc cttcatccac accatctgcc ctctgcagga
      841 aaacagctct ggcgaggaca ttttcaccaa gcttctggac ctgagggagc ccacgtggaa
      901 gcagtggaga gaattcctcg tcaagtactc ctttcttccg taccagctga tcgcggagtt
      961 tgcctgggac tggctggagg tccactactg gacatctcgc ttcctgatcg tcaatgccgt
     1021 gctgctctct gttctggagt tgttctcttt ttggaggatc tggtcgagaa gtgagctgaa
     1081 gacagtgccc cagaggatgt ggagccactt ctggaaagta tcgacacagg ggcttttcat
     1141 ggccatgttc tggccgctta ttcctcagtt tgtctgcaac tgtttatttt actgggctct
     1201 gtacttcaac ccaattatta acattgatct ggtggtcaag gaagtccgac ggctggaaac
     1261 ccaagtgtta tgatgggcag caaggcccga gcgagcccgt gaatctacct gacaaccaag
     1321 ctgtgacatt caaagagaca agggactcgt ttctacccct ggtaatgcga ggtgctgctt
     1381 ctgtgtgtca aggcttcaac caggtccctc ttcctctgac tctggcacca tgccagctga
     1441 ctgctaccca ggtcagattg gactcatggt caatacccag ccagtcttct tacagggact
     1501 tgagaggctc aggctactat gaccaataga agtgaaagtc ttggtcataa tttggggtga
     1561 catgctgtca aaaggctaat atagactggg tgtggtagtg cacaccttga atctgagcac
     1621 taggaaaaca ggcaggtgga tttttgagtt caagaccaga gtggtctaca gagtgagttc
     1681 tacaacagcc agggctacat agaggaacca tgtctctaaa aaaaaaaaaa aagaaagaaa
     1741 gaaaaagaaa agaaaaaaga aagaaatcaa aacaaaacaa aaagaagaaa agataaaaag
     1801 gaaggccatt atggtcagta gccctaacct cactaactga catactccca aaccaggctt
     1861 cttgggccag gtctgtccca tgagtcaaat ttaggaaagc aaggataggt tttgtcatag
     1921 atatggatag ttggacatac aaaataaaga cctagaatcc agtcatagtt tctcactgaa
     1981 tagttggtag gtttcagaat aaactcagag aaaacttcag gctaagagac agtgctgcgt
     2041 agctgtgttg tttcctgtta tgagcattgg aaattgggtt ctccttctgt gagcatggag
     2101 ttgaatggaa tgcccaccag gccgagtctt cttagatgac tgagcactgc ttgatgttgt
     2161 gttttgaata atttaatctg tacaataaat acagtatagg ctgatcaagg agcattttgt
     2221 aagctgccct cacactatat aatggacaat ctttattctg ttctttagta taacagcaag
     2281 tataattatg ccgagtcctg ccatcataag tatggagtaa gctaaaggat gaccgttatg
     2341 cccgtgtaga catagtgtac tcagcagatg tagaatgtga gctcatcagc cttggcctgg
     2401 ctgaaacagg cttgcatcca acgtgagacc gacagacgga cagacggaca gacagaatgt
     2461 atgggtgaga gagagacaga gacaatggga gagagacaga aggagagagt cagagagact
     2521 tggtaattca ttaactcttt gtatctcaga gtgaagattt gacttaaact ttgcattgaa
     2581 agtttggaaa attacttgaa ataaaaaaag atttttatac aaatacaaaa aaaaa
//
