LOCUS       NM_133217               2093 bp    mRNA    linear   ROD 01-OCT-2024
DEFINITION  Mus musculus beta-carotene oxygenase 2 (Bco2), transcript variant
            1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_133217 XM_993021
VERSION     NM_133217.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Miller,A.P., Hornero-Mendez,D., Bandara,S., Parra-Rivero,O.,
            Limon,M.C., von Lintig,J., Avalos,J. and Amengual,J.
  TITLE     Bioavailability and provitamin A activity of neurosporaxanthin in
            mice
  JOURNAL   Commun Biol 6 (1), 1068 (2023)
   PUBMED   37864015
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Thomas,L.D., Ramkumar,S., Golczak,M. and von Lintig,J.
  TITLE     Genetic deletion of Bco2 and Isx establishes a golden mouse model
            for carotenoid research
  JOURNAL   Mol Metab 73, 101742 (2023)
   PUBMED   37225015
REFERENCE   3  (bases 1 to 2093)
  AUTHORS   Li,B., Vachali,P., Chang,F.Y., Gorusupudi,A., Arunkumar,R., Shi,L.,
            Rognon,G.T., Frederick,J.M. and Bernstein,P.S.
  TITLE     HDL is the primary transporter for carotenoids from liver to
            retinal pigment epithelium in transgenic ApoA-I-/-/Bco2-/- mice
  JOURNAL   Arch Biochem Biophys 716, 109111 (2022)
   PUBMED   34942193
REFERENCE   4  (bases 1 to 2093)
  AUTHORS   Miller,A.P., Black,M. and Amengual,J.
  TITLE     Fenretinide inhibits vitamin A formation from beta-carotene and
            regulates carotenoid levels in mice
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1867 (2), 159070 (2022)
   PUBMED   34742949
REFERENCE   5  (bases 1 to 2093)
  AUTHORS   Arunkumar,R., Gorusupudi,A., Li,B., Blount,J.D., Nwagbo,U.,
            Kim,H.J., Sparrow,J.R. and Bernstein,P.S.
  TITLE     Lutein and zeaxanthin reduce A2E and iso-A2E levels and improve
            visual performance in Abca4-/-/Bco2-/- double knockout mice
  JOURNAL   Exp Eye Res 209, 108680 (2021)
   PUBMED   34161819
  REMARK    GeneRIF: Lutein and zeaxanthin reduce A2E and iso-A2E levels and
            improve visual performance in Abca4(-/-)/Bco2(-/-) double knockout
            mice.
REFERENCE   6  (bases 1 to 2093)
  AUTHORS   Amengual,J., Lobo,G.P., Golczak,M., Li,H.N., Klimova,T.,
            Hoppel,C.L., Wyss,A., Palczewski,K. and von Lintig,J.
  TITLE     A mitochondrial enzyme degrades carotenoids and protects against
            oxidative stress
  JOURNAL   FASEB J 25 (3), 948-959 (2011)
   PUBMED   21106934
  REMARK    GeneRIF: we found that the third family member,
            beta,beta-carotene-9',10'-oxygenase (BCDO2), is a mitochondrial
            carotenoid-oxygenase with broad substrate specificity
REFERENCE   7  (bases 1 to 2093)
  AUTHORS   Shmarakov,I., Fleshman,M.K., D'Ambrosio,D.N., Piantedosi,R.,
            Riedl,K.M., Schwartz,S.J., Curley,R.W. Jr., von Lintig,J.,
            Rubin,L.P., Harrison,E.H. and Blaner,W.S.
  TITLE     Hepatic stellate cells are an important cellular site for
            beta-carotene conversion to retinoid
  JOURNAL   Arch Biochem Biophys 504 (1), 3-10 (2010)
   PUBMED   20470748
REFERENCE   8  (bases 1 to 2093)
  AUTHORS   Ford,N.A., Clinton,S.K., von Lintig,J., Wyss,A. and Erdman,J.W. Jr.
  TITLE     Loss of carotene-9',10'-monooxygenase expression increases serum
            and tissue lycopene concentrations in lycopene-fed mice
  JOURNAL   J Nutr 140 (12), 2134-2138 (2010)
   PUBMED   20962153
REFERENCE   9  (bases 1 to 2093)
  AUTHORS   Hu,K.Q., Liu,C., Ernst,H., Krinsky,N.I., Russell,R.M. and Wang,X.D.
  TITLE     The biochemical characterization of ferret
            carotene-9',10'-monooxygenase catalyzing cleavage of carotenoids in
            vitro and in vivo
  JOURNAL   J Biol Chem 281 (28), 19327-19338 (2006)
   PUBMED   16672231
REFERENCE   10 (bases 1 to 2093)
  AUTHORS   Kiefer,C., Hessel,S., Lampert,J.M., Vogt,K., Lederer,M.O.,
            Breithaupt,D.E. and von Lintig,J.
  TITLE     Identification and characterization of a mammalian enzyme
            catalyzing the asymmetric oxidative cleavage of provitamin A
  JOURNAL   J Biol Chem 276 (17), 14110-14116 (2001)
   PUBMED   11278918
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127254.3, BB624957.1, BC107006.2 and CB527507.1.
            
            On Aug 25, 2007 this sequence version replaced NM_133217.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AJ290392.1, BC107006.2 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMN00849374,
                                    SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               AC127254.3         174245-174362       c
            119-175             BB624957.1         164-220
            176-1815            BC107006.2         19-1658
            1816-2093           CB527507.1         208-485
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 27.74 cM"
     gene            1..2093
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /note="beta-carotene oxygenase 2"
                     /db_xref="GeneID:170752"
                     /db_xref="MGI:MGI:2177469"
     exon            1..118
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            119..321
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..1756
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /EC_number="1.13.11.71"
                     /note="isoform 1 is encoded by transcript variant 1;
                     beta-carotene 9', 10'-dioxygenase 2;
                     b,b-carotene-9',10'-dioxygenase; beta,beta-carotene
                     9',10'-oxygenase; beta-carotene dioxygenase 2;
                     beta-diox-II; carotenoid-cleaving dioxygenase,
                     mitochondrial"
                     /codon_start=1
                     /product="carotenoid-cleaving dioxygenase, mitochondrial
                     isoform 1"
                     /protein_id="NP_573480.1"
                     /db_xref="CCDS:CCDS40620.1"
                     /db_xref="GeneID:170752"
                     /db_xref="MGI:MGI:2177469"
                     /translation="MLGPKQSLPCIAPLLTTAEETLSAVSARVRGHIPEWLNGYLLRV
                     GPGKFEFGKDRYNHWFDGMALLHQFRMERGTVTYKSKFLQSDTYKANSAGGRIVISEF
                     GTLALPDPCKSIFERFMSRFEPPTMTDNTNVNFVQYKGDYYMSTETNFMNKVDIEMLE
                     RTEKVDWSKFIAVNGATAHPHYDPDGTAYNMGNSYGPRGSCYNIIRVPPKKKEPGETI
                     HGAQVLCSIASTEKMKPSYYHSFGMTKNYIIFVEQPVKMKLWKIITSKIRGKPFADGI
                     SWEPQYNTRFHVVDKHTGQLLPGMYYSMPFLTYHQINAFEDQGCIVIDLCCQDDGRSL
                     DLYQLQNLRKAGEGLDQVYELKAKSFPRRFVLPLDVSVDAAEGKNLSPLSYSSASAVK
                     QGDGEIWCSPENLHHEDLEEEGGIEFPQINYGRFNGKKYSFFYGCGFRHLVGDSLIKV
                     DVTNKTLRVWREEGFYPSEPVFVPVPGADEEDSGVILSVVITPNQSESNFLLVLDAKS
                     FTELGRAEVPVQMPYGFHGTFVPI"
     exon            322..533
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            534..649
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            650..752
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            753..881
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1042
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1210
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1348
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1531
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1642
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..2075
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2052..2057
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /note="hexamer: ATTAAA"
     polyA_site      2075
                     /gene="Bco2"
                     /gene_synonym="B-diox-II; Bcdo2; Bcmo2; CMO2"
                     /note="major polyA site"
ORIGIN      
        1 aatctcaaga cctgagttaa gaactgagtt aaatactatt tctcaactgt ttctgcgttt
       61 cctcagcagc cttcttgaca ccgcagggtt cttcctgtgg cagtgtacca tcgcccagtt
      121 ttgaaggagt atgtgaggga atactcacca ggaaaccatg ttgggaccga agcagagcct
      181 gccatgcatt gccccactgc tgaccacggc ggaggagact ctgagtgctg tctctgctcg
      241 ggtccgagga catattcctg aatggcttaa tggttatcta cttcgagttg gacctgggaa
      301 gtttgaattt gggaaggata gatacaatca ttggtttgat ggaatggcgt tgcttcacca
      361 gttccgaatg gagaggggca cagtgacata caagagcaag tttctacaga gtgacacata
      421 taaggccaac agtgctggag gtagaattgt gatctcagaa tttggcacgc tggcccttcc
      481 tgacccatgc aagagcatct ttgaacgttt catgtcaagg tttgagccac ctactatgac
      541 tgacaacacc aacgtcaact ttgtgcagta caaaggtgat tactacatga gcacagagac
      601 taattttatg aataaggtgg acattgagat gctggaaagg acagaaaagg tggactggag
      661 caaattcatt gctgtgaatg gagccactgc acatcctcat tacgacccag atgggacagc
      721 atacaacatg gggaacagct atgggccaag aggttcttgc tataatatta ttcgtgttcc
      781 tccaaaaaag aaagagcccg gggagacgat tcacggagca caggtgctat gttccattgc
      841 ctccactgag aaaatgaagc cttcttacta ccatagcttt ggaatgacaa aaaactacat
      901 aatctttgtc gaacagcctg taaagatgaa gctgtggaaa ataatcactt ctaaaatccg
      961 gggaaagccc tttgctgatg ggataagctg ggagccccag tataacacgc ggtttcatgt
     1021 ggtggataaa cacactggac agcttctccc aggaatgtac tacagcatgc cttttcttac
     1081 ctatcatcaa atcaatgcct ttgaggacca gggctgtatt gtgattgatc tgtgctgcca
     1141 ggatgatggg agaagcctag acctttacca actacagaat ctcaggaaag ctggagaggg
     1201 gcttgatcag gtctatgagt taaaggcaaa gtctttccct cgaagatttg tcttgccctt
     1261 agatgttagt gtggatgctg ctgaaggaaa gaacctcagc ccactgtcct attcttcagc
     1321 cagcgctgtg aaacagggtg atggagagat ctggtgctct cctgaaaatc tacaccacga
     1381 agacctggaa gaggaagggg ggattgaatt ccctcagatc aactatggcc gattcaatgg
     1441 caaaaagtat agtttcttct atggctgcgg ttttcgacat ttggtggggg attctctgat
     1501 taaggttgac gtgacgaaca agacactaag ggtttggaga gaagaaggct tttatccctc
     1561 ggagcccgtt tttgttccgg tgccaggagc agatgaggaa gacagtgggg ttatactctc
     1621 tgtggtgatc actcccaacc agagtgaaag caacttcctc cttgtcttgg atgccaagag
     1681 cttcacagag ctggggcgag cggaagtacc cgtgcagatg ccttacgggt tccatggcac
     1741 ctttgtgcct atctgacggc agaggcgcaa ggaaggctag gatcgggctt cgatgagcac
     1801 actctgagga aaagagaaaa tggtggatct cactcaaaag ctgttgtagt ttggacctga
     1861 ccctgacccc taaggactca tagacccgac tcccgtgggc tcatcgaccc tgacccccaa
     1921 cgtgctgata gatcctgacc accacgggat catatttaaa ttcttgttcc cagcttgtgg
     1981 cactactttt tttttttttg tagcagtggt aaaataacta ataatctccc catgtatggt
     2041 tttatatttc tattaaaaca caacttgtga ggtaaaaaaa aaaaaaaaaa aaa
//
