LOCUS       NM_009739               2857 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Mus musculus branched chain ketoacid dehydrogenase kinase (Bckdk),
            mRNA.
ACCESSION   NM_009739
VERSION     NM_009739.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Zhou,F., Sheng,C., Ma,X., Li,T., Ming,X., Wang,S., Tan,J., Yang,Y.,
            Sun,H., Lu,J., Liu,J., Deng,R., Wang,X. and Zhou,L.
  TITLE     BCKDH kinase promotes hepatic gluconeogenesis independent of BCKDHA
  JOURNAL   Cell Death Dis 15 (10), 736 (2024)
   PUBMED   39389936
  REMARK    GeneRIF: BCKDH kinase promotes hepatic gluconeogenesis independent
            of BCKDHA.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Murashige,D., Jung,J.W., Neinast,M.D., Levin,M.G., Chu,Q.,
            Lambert,J.P., Garbincius,J.F., Kim,B., Hoshino,A., Marti-Pamies,I.,
            McDaid,K.S., Shewale,S.V., Flam,E., Yang,S., Roberts,E., Li,L.,
            Morley,M.P., Bedi,K.C. Jr., Hyman,M.C., Frankel,D.S.,
            Margulies,K.B., Assoian,R.K., Elrod,J.W., Jang,C., Rabinowitz,J.D.
            and Arany,Z.
  TITLE     Extra-cardiac BCAA catabolism lowers blood pressure and protects
            from heart failure
  JOURNAL   Cell Metab 34 (11), 1749-1764 (2022)
   PUBMED   36223763
REFERENCE   3  (bases 1 to 2857)
  AUTHORS   Heinemann-Yerushalmi,L., Bentovim,L., Felsenthal,N.,
            Vinestock,R.C., Michaeli,N., Krief,S., Silberman,A., Cohen,M.,
            Ben-Dor,S., Brenner,O., Haffner-Krausz,R., Itkin,M., Malitsky,S.,
            Erez,A. and Zelzer,E.
  TITLE     BCKDK regulates the TCA cycle through PDC in the absence of PDK
            family during embryonic development
  JOURNAL   Dev Cell 56 (8), 1182-1194 (2021)
   PUBMED   33773101
  REMARK    GeneRIF: BCKDK regulates the TCA cycle through PDC in the absence
            of PDK family during embryonic development.
REFERENCE   4  (bases 1 to 2857)
  AUTHORS   Mizusawa,A., Watanabe,A., Yamada,M., Kamei,R., Shimomura,Y. and
            Kitaura,Y.
  TITLE     BDK Deficiency in Cerebral Cortex Neurons Causes Neurological
            Abnormalities and Affects Endurance Capacity
  JOURNAL   Nutrients 12 (8), 2267 (2020)
   PUBMED   32751134
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2857)
  AUTHORS   Neinast,M.D., Jang,C., Hui,S., Murashige,D.S., Chu,Q.,
            Morscher,R.J., Li,X., Zhan,L., White,E., Anthony,T.G.,
            Rabinowitz,J.D. and Arany,Z.
  TITLE     Quantitative Analysis of the Whole-Body Metabolic Fate of
            Branched-Chain Amino Acids
  JOURNAL   Cell Metab 29 (2), 417-429 (2019)
   PUBMED   30449684
REFERENCE   6  (bases 1 to 2857)
  AUTHORS   Joshi,M.A., Jeoung,N.H., Obayashi,M., Hattab,E.M., Brocken,E.G.,
            Liechty,E.A., Kubek,M.J., Vattem,K.M., Wek,R.C. and Harris,R.A.
  TITLE     Impaired growth and neurological abnormalities in branched-chain
            alpha-keto acid dehydrogenase kinase-deficient mice
  JOURNAL   Biochem J 400 (1), 153-162 (2006)
   PUBMED   16875466
  REMARK    GeneRIF: branched-chain a-keto acid dehydrogenase complex kinase
            plays an important role in conserving branched-chain amino acids by
            limiting their oxidation
REFERENCE   7  (bases 1 to 2857)
  AUTHORS   Trinidad,J.C., Specht,C.G., Thalhammer,A., Schoepfer,R. and
            Burlingame,A.L.
  TITLE     Comprehensive identification of phosphorylation sites in
            postsynaptic density preparations
  JOURNAL   Mol Cell Proteomics 5 (5), 914-922 (2006)
   PUBMED   16452087
REFERENCE   8  (bases 1 to 2857)
  AUTHORS   Muller,E.A. and Danner,D.J.
  TITLE     Tissue-specific translation of murine branched-chain alpha-ketoacid
            dehydrogenase kinase mRNA is dependent upon an upstream open
            reading frame in the 5'-untranslated region
  JOURNAL   J Biol Chem 279 (43), 44645-44655 (2004)
   PUBMED   15302860
  REMARK    GeneRIF: Tissue-specific translation of murine BCKDK mRNA is
            dependent upon an upstream open reading frame in the
            5'-untranslated region
REFERENCE   9  (bases 1 to 2857)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   10 (bases 1 to 2857)
  AUTHORS   Doering,C.B., Coursey,C., Spangler,W. and Danner,D.J.
  TITLE     Murine branched chain alpha-ketoacid dehydrogenase kinase; cDNA
            cloning, tissue distribution, and temporal expression during
            embryonic development
  JOURNAL   Gene 212 (2), 213-219 (1998)
   PUBMED   9611264
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY077717.1 and AK150607.1.
            
            On Feb 25, 2006 this sequence version replaced NM_009739.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK150607.1, AK167519.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            regulatory uORF                    :: PMID: 15302860
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BY077717.1         2-10
            10-2857             AK150607.1         2-2849
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.82 cM"
     gene            1..2857
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /note="branched chain ketoacid dehydrogenase kinase"
                     /db_xref="GeneID:12041"
                     /db_xref="MGI:MGI:1276121"
     exon            1..97
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            98..460
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      98..157
                     /regulatory_class="uORF"
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:15302860]"
                     /note="this uORF is predicted to encode a 19 aa peptide
                     (PMID:15302860)"
     CDS             266..1504
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.4"
                     /note="[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]]
                     kinase, mitochondrial; BCKDHKIN; branched-chain
                     alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDH
                     kinase"
                     /codon_start=1
                     /product="branched-chain alpha-ketoacid dehydrogenase
                     kinase precursor"
                     /protein_id="NP_033869.1"
                     /db_xref="CCDS:CCDS21884.1"
                     /db_xref="GeneID:12041"
                     /db_xref="MGI:MGI:1276121"
                     /translation="MILTSVLGSGPRSWSSLWPLLGSSLSLRARSTSATDTHHVELAR
                     ERSKTVTSFYNQSAIDVAAEKPSVRLTPTMMLYSGRSQDGSHLLKSGRYLQQELPVRI
                     AHRIKGFRSLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLD
                     DHKDVVTLLAEGLRESRKHIQDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFV
                     GIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPEL
                     LKNAMRATMESHLDTPYNVPDVVITIANNDIDLIIRISDRGGGIAHKDLDRVMDYHFT
                     TAEASTQDPRINPLFGHLDMHSGGQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGI
                     GTDVYLRLRHIDGREESFRI"
     exon            461..529
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            530..640
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            641..688
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            689..808
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            809..907
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            908..981
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1110
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1200
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1359
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..2857
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1777..1782
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /note="hexamer: ATTAAA"
     polyA_site      1816
                     /gene="Bckdk"
                     /gene_synonym="BDK"
                     /note="major polyA site"
ORIGIN      
        1 gttacagccg gggagggcga aggcgggctc ggctagccga gcgggtcgac tgctcccgcg
       61 gttccgctcg aacccgggcc cagcgtctgg cctcgcgatg gaagctgctg tgcgggcgct
      121 gagcacgtct gcatcctcga gggaaccaca accctgaaat ccggagaaaa tcccgcccac
      181 ccactttctc tttactccca ctttggggtc accattgcct gtcgggtgct atagtgtcac
      241 cctagttcaa caaagtagca ggacaatgat actgacttca gtgctgggca gcggccctcg
      301 gagctggtct tcactttggc ccctcttggg gtcctcactg tcactccggg ctcgctcaac
      361 atcagccacc gatacacacc atgtagagct ggccagggaa cgctccaaga ctgtcacctc
      421 tttttacaac cagtcagcca ttgatgtggc agcagagaag ccctcagttc gcctcactcc
      481 caccatgatg ctctattccg gtcgctcaca ggatggcagc caccttctga aaagtggccg
      541 ctacttgcag caagagttac cagtgaggat tgctcaccgc atcaagggct tccgtagcct
      601 tcctttcatc attggttgca accctaccat actgcatgtg cacgagctat acatccgggc
      661 cttccagaag ctgacagact tccctccgat caaggaccag gcagatgagg cccagtactg
      721 ccagctggtg agacagctgc tagatgacca caaggatgtg gtgaccctgt tagctgaggg
      781 tcttcgtgag agccggaaac acatccagga tgaaaagcta gtccggtact tcttggataa
      841 aacactgacg tcaagacttg ggatccgaat gctggctact caccacttgg cgctacatga
      901 agacaagcct gattttgttg gcatcatctg tactcgtctg tcgcccaaga agattatcga
      961 gaagtgggtg gattttgcca gacgcctgtg tgagcacaag tatggcaatg cccctcgagt
     1021 ccgcatcaac ggacatgtgg ctgcccggtt ccccttcatt cccatgccgc tggactatat
     1081 cctgcctgag ctgctcaaga atgccatgag agccacaatg gagagtcacc tagacactcc
     1141 ctacaatgtc ccagatgtgg tcatcaccat cgccaataat gatattgatc tcatcatcag
     1201 gatctcagac cggggtggag gaatcgctca taaggacctg gatcgggtca tggactacca
     1261 cttcaccaca gctgaggcca gcacccagga cccccgaatc aaccccctct tcggccacct
     1321 ggatatgcac agtggtggcc agtcaggacc tatgcatggc tttggcttcg ggttgcccac
     1381 gtccagggca tatgcagagt atcttggtgg ctccctgcag ctgcagtccc tgcagggcat
     1441 tggcacggat gtctacctac ggctccgcca cattgatggt cgggaggaaa gcttcagaat
     1501 ctaatggtgg attttggccg gctagcctgc cacactcctc ggaggacctc ctaggcctgg
     1561 cagggggccg ctgggctcca cagactgcca cttcgcttta tggagcttag ggcctcagac
     1621 aggtggactt agatggagct gggcaccact cctcctcagc agggctcact gctttctacc
     1681 tcccggcctt ggggggagga agcaggtgcc tctgaggcct ctgataccag ctctgctctg
     1741 ctcattcttg aggactcctc atttgacctg ctgcttatta aagtttgcat ttttgaatgc
     1801 cctcttgggc cccaaatgtt gggagggcag gcgagctttg tttctgcccc ctttggattc
     1861 cctaggcctt gggagttaac ctggtcatgt ccagtctctg gctgtcctgg cactgtggca
     1921 gaagtgattt caaggttctt gcctatttga tttggcaaga gtgagagtga ctttggcaac
     1981 ttgggctcat tgaacaagtc tttctgggcc ccagacgcta tcagaggccc caaaagactt
     2041 agcttcaggc ccgactcttc ccttcctggc agccgtgggt ttgagaggta aaggtcacta
     2101 gactgggcag tctgtaagcc acctgattcc tgagcttgca tgaggttaaa aatactgcct
     2161 gcccactgtg gctgcaaaaa gaattgcatg aggttatgca gagacctgat tacctcagac
     2221 agatacggag aagttgtctt agggaggacc agggacttgg gcagaaagca cggtgaccag
     2281 aagtgtgtgg aacagaactt taggtgaact ttgaaccaag agtaactctc cccgctgtgt
     2341 aagacctacg gacctgcttg gtaggactcc caaaagggag taggagaagc aggaaaggaa
     2401 aggggagagg tcaaggaaat gacttgtaat gagaagtcca gagctagtcc actgtccctc
     2461 aggtacacat aagagagtga ggctccatgg taaccagttc cctcacttga cgctgggatg
     2521 gaagaaattc ctacactgaa aactttagcc tttccaggta gaatcaaatg tacacacttg
     2581 tggtcctagt actagtgatg cttacatagg agtatcacga gttcaaggcc aacctgggct
     2641 gcttggcaaa accctgtctt aacgggtagt tgtcagagaa gagcctcatg ttctagtctt
     2701 tgaatgtaca tacagaccgg gggtggtggc acacgccttt aatcccagca cttgggaggc
     2761 agaggcaggc ggatttctga gttcaaggcc agcctgatct acagagtgag ttccaggaca
     2821 gccaggacta tacagagaaa ccctgtctcg aaaaacc
//
