LOCUS       NM_001412000            1653 bp    mRNA    linear   ROD 13-MAY-2024
DEFINITION  Mus musculus branched chain aminotransferase 2, mitochondrial
            (Bcat2), transcript variant 4, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001412000
VERSION     NM_001412000.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wang,Y., Zhao,N., Meng,Y., Chen,J., Qi,C., Hu,X., Zhu,H., Yang,D.,
            Zhang,X., Ma,H., Zhao,J., Di,T., Li,P. and Wang,Y.
  TITLE     Bcat2-Mediated Branched-Chain Amino Acid Catabolism Is Linked to
            the Aggravated Inflammation in Obese with Psoriasis Mice
  JOURNAL   Mol Nutr Food Res 68 (8), e2300720 (2024)
   PUBMED   38581348
  REMARK    GeneRIF: Bcat2-Mediated Branched-Chain Amino Acid Catabolism Is
            Linked to the Aggravated Inflammation in Obese with Psoriasis Mice.
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Portero,V., Nicol,T., Podliesna,S., Marchal,G.A., Baartscheer,A.,
            Casini,S., Tadros,R., Treur,J.L., Tanck,M.W.T., Cox,I.J.,
            Probert,F., Hough,T.A., Falcone,S., Beekman,L., Muller-Nurasyid,M.,
            Kastenmuller,G., Gieger,C., Peters,A., Kaab,S., Sinner,M.F.,
            Blease,A., Verkerk,A.O., Bezzina,C.R., Potter,P.K. and Remme,C.A.
  TITLE     Chronically elevated branched chain amino acid levels are
            pro-arrhythmic
  JOURNAL   Cardiovasc Res 118 (7), 1742-1757 (2022)
   PUBMED   34142125
REFERENCE   3  (bases 1 to 1653)
  AUTHORS   Uddin,G.M., Karwi,Q.G., Pherwani,S., Gopal,K., Wagg,C.S.,
            Biswas,D., Atnasious,M., Wu,Y., Wu,G., Zhang,L., Ho,K.L.,
            Pulinilkunnil,T., Ussher,J.R. and Lopaschuk,G.D.
  TITLE     Deletion of BCATm increases insulin-stimulated glucose oxidation in
            the heart
  JOURNAL   Metabolism 124, 154871 (2021)
   PUBMED   34478752
  REMARK    GeneRIF: Deletion of BCATm increases insulin-stimulated glucose
            oxidation in the heart.
REFERENCE   4  (bases 1 to 1653)
  AUTHORS   Li,J.T., Yin,M., Wang,D., Wang,J., Lei,M.Z., Zhang,Y., Liu,Y.,
            Zhang,L., Zou,S.W., Hu,L.P., Zhang,Z.G., Wang,Y.P., Wen,W.Y.,
            Lu,H.J., Chen,Z.J., Su,D. and Lei,Q.Y.
  TITLE     BCAT2-mediated BCAA catabolism is critical for development of
            pancreatic ductal adenocarcinoma
  JOURNAL   Nat Cell Biol 22 (2), 167-174 (2020)
   PUBMED   32029896
REFERENCE   5  (bases 1 to 1653)
  AUTHORS   Koike,H., Zhang,R.R., Ueno,Y., Sekine,K., Zheng,Y.W., Takebe,T. and
            Taniguchi,H.
  TITLE     Nutritional modulation of mouse and human liver bud growth through
            a branched-chain amino acid metabolism
  JOURNAL   Development 144 (6), 1018-1024 (2017)
   PUBMED   28219950
REFERENCE   6  (bases 1 to 1653)
  AUTHORS   Wu,J.Y., Kao,H.J., Li,S.C., Stevens,R., Hillman,S., Millington,D.
            and Chen,Y.T.
  TITLE     ENU mutagenesis identifies mice with mitochondrial branched-chain
            aminotransferase deficiency resembling human maple syrup urine
            disease
  JOURNAL   J Clin Invest 113 (3), 434-440 (2004)
   PUBMED   14755340
REFERENCE   7  (bases 1 to 1653)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 1653)
  AUTHORS   Ben-Yosef,T., Eden,A. and Benvenisty,N.
  TITLE     Characterization of murine BCAT genes: Bcat1, a c-Myc target, and
            its homolog, Bcat2
  JOURNAL   Mamm Genome 9 (7), 595-597 (1998)
   PUBMED   9657861
REFERENCE   9  (bases 1 to 1653)
  AUTHORS   Bledsoe,R.K., Dawson,P.A. and Hutson,S.M.
  TITLE     Cloning of the rat and human mitochondrial branched chain
            aminotransferases (BCATm)
  JOURNAL   Biochim Biophys Acta 1339 (1), 9-13 (1997)
   PUBMED   9165094
REFERENCE   10 (bases 1 to 1653)
  AUTHORS   Bassett,D.E. Jr., Boguski,M.S., Spencer,F., Reeves,R., Kim,S.,
            Weaver,T. and Hieter,P.
  TITLE     Genome cross-referencing and XREFdb: implications for the
            identification and analysis of genes mutated in human disease
  JOURNAL   Nat Genet 15 (4), 339-344 (1997)
   PUBMED   9090377
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151602.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253011.5875257.1,
                                           SRR17253013.4374743.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AC151602.4         20065-20115         c
            52-129              AC151602.4         15274-15351         c
            130-204             AC151602.4         14959-15033         c
            205-408             AC151602.4         14462-14665         c
            409-519             AC151602.4         5437-5547           c
            520-639             AC151602.4         5234-5353           c
            640-803             AC151602.4         4887-5050           c
            804-946             AC151602.4         2673-2815           c
            947-1032            AC151602.4         2444-2529           c
            1033-1173           AC151602.4         2085-2225           c
            1174-1248           AC151602.4         1936-2010           c
            1249-1653           AC151602.4         754-1158            c
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.36 cM"
     gene            1..1653
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /note="branched chain aminotransferase 2, mitochondrial"
                     /db_xref="GeneID:12036"
                     /db_xref="MGI:MGI:1276534"
     exon            1..51
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1287
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /EC_number="2.6.1.42"
                     /note="isoform 3 is encoded by transcript variant 4;
                     branched-chain-amino-acid aminotransferase, mitochondrial"
                     /codon_start=1
                     /product="branched-chain-amino-acid aminotransferase,
                     mitochondrial isoform 3"
                     /protein_id="NP_001398929.1"
                     /db_xref="GeneID:12036"
                     /db_xref="MGI:MGI:1276534"
                     /translation="MAAATLGQECSLTCSLSLVVVPSKLSTSCGQSSLVWARKLLPVP
                     WLLCGSKRCVSSIFKAADLQIQMTKEPQKKPAPSQALLFGKTFTDHMLMVEWNNKAGW
                     GPPRIQPFQNLTLHPACSGLHYSLQLFEGLKAYKGGDQQVRLFRPWLNMDRMLRSARR
                     LCLPDFDKQELLECIRQLIEVDKDWVPDGNGTSLYVRPVLIGNEPSLGVGMVTQALLY
                     VILCPVGSYFPGDSMTPVSLLADPSFVRAWIGGVGDCKLGGNYGPTVAVQREAQKRGC
                     EQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDLART
                     WGEFRVAERKVTMKELKRALEEGRVREVFGSGTACQVCPVHQILYEGKQLHIPTMENG
                     PELILRFQKELKAIQYGASAHDWMFRV"
     exon            52..129
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            130..204
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            205..408
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            409..519
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            520..639
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            640..803
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            804..946
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1032
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1173
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1248
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1653
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1632..1637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /note="hexamer: AATAAA"
     polyA_site      1653
                     /gene="Bcat2"
                     /gene_synonym="Bcat(m); Bcat-2; Eca40"
                     /note="major polyA site"
ORIGIN      
        1 acagtcacca gccgcacaca ccggatcatg gctgcagcca cactaggaca ggagtgttcc
       61 cttacctgct cgctgtccct ggtcgttgtc ccgtctaagc tgtctacaag ctgcggccaa
      121 agttctctag tctgggcccg aaagcttctg cctgttccct ggcttctatg tgggtccaaa
      181 agatgtgtgt cctccatatt caaggctgca gacctccaga ttcagatgac caaagaacca
      241 cagaagaagc cagcccctag ccaggcactg ctgtttggga agaccttcac agaccacatg
      301 ctgatggtgg agtggaataa caaggctgga tggggcccac caaggatcca gcccttccag
      361 aacctcacgc tacacccagc ctgctctggt ctgcactact ctctgcagct ctttgagggc
      421 ttgaaagcat acaaaggtgg agaccagcag gtacgcctct tccggccctg gctcaacatg
      481 gacaggatgc tacgttctgc taggcgcctc tgcctgccag actttgacaa gcaggagctg
      541 ttggagtgta tccgccagct cattgaagta gacaaagact gggttcccga tggcaacgga
      601 acgagcctct acgtgcggcc cgtgctgatc gggaatgagc cctcgctggg tgttggtatg
      661 gtcacacaag ccctcctgta cgtcattctg tgccctgtgg gctcctactt ccctggagac
      721 tccatgacac ctgtctccct cctggcggac ccttcattcg tcagagcctg gataggcgga
      781 gtcggtgact gcaagttggg ggggaactat ggacccactg tggctgtgca gcgagaagcc
      841 cagaagagag gctgcgagca ggtcctctgg ctgtacgggc cagaccacca gctcactgag
      901 gtgggcacca tgaacatctt tgtctactgg acccatgaag acggagtact ggagctggta
      961 acccccccac tgaatggcgt catcttgcct ggagtagttc gacaaagtct gctggatctg
     1021 gccaggactt ggggtgagtt ccgggtggca gaacgcaagg tcactatgaa ggaattgaag
     1081 cgggcgctgg aggaaggccg agtgcgagaa gtcttcggct caggcactgc ttgtcaggtc
     1141 tgcccggtgc accaaatcct gtatgaaggc aagcaactcc acatacctac catggagaac
     1201 ggcccagagc tcatcctgcg cttccagaag gaactgaagg ctattcagta cggagccagt
     1261 gcccacgatt ggatgtttcg ggtgtgacgg tgggggctgt gccctccctg gttggatctg
     1321 cagacgtgcc gcatctagtc cagcgtcctc gcttcccaga gactcaagag aagtgcaata
     1381 gggaaaggtc tgtccagatg ctggatctct ggcaccccct cgtggacgtt acactccaaa
     1441 gccttaaggg cgggacccat gcaatttgga cgccacctca aattttgggt ctcaggactc
     1501 agctgctccg cgtcctggtg ttcaagggag aaccttggct tcttctctat ctccgttctc
     1561 aggccggatc cccggtgctc ttcgtgtcct tccccccccc ccccttttct ccgtacttgc
     1621 tgcaattttg aaataaaatg ccaaagaaca aga
//
