LOCUS       NM_001171739            1365 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus BCL2-associated athanogene 1 (Bag1), transcript
            variant 1, mRNA.
ACCESSION   NM_001171739
VERSION     NM_001171739.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Neeb,A., Figueiredo,I., Bogdan,D., Cato,L., Stober,J.,
            Jimenez-Vacas,J.M., Gourain,V., Lee,I.I., Seeger,R., Muhle-Goll,C.,
            Gurel,B., Welti,J., Nava Rodrigues,D., Rekowski,J., Qiu,X.,
            Jiang,Y., Di Micco,P., Mateos,B., Bielskute,S., Riisnaes,R.,
            Ferreira,A., Miranda,S., Crespo,M., Buroni,L., Ning,J.,
            Carreira,S., Brase,S., Jung,N., Grassle,S., Swain,A.,
            Salvatella,X., Plymate,S.R., Al-Lazikani,B., Long,H.W., Yuan,W.,
            Brown,M., Cato,A.C.B., de Bono,J.S. and Sharp,A.
  TITLE     Thio-2 Inhibits Key Signaling Pathways Required for the Development
            and Progression of Castration-resistant Prostate Cancer
  JOURNAL   Mol Cancer Ther 23 (6), 791-808 (2024)
   PUBMED   38412481
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Inose-Maruyama,A., Irokawa,H., Takeda,K., Taguchi,K., Morita,M.,
            Yamamoto,M., Sasaki,M. and Kuge,S.
  TITLE     Bag1 protein loss sensitizes mouse embryonic fibroblasts to
            glutathione depletion
  JOURNAL   Cell Stress Chaperones 29 (3), 497-509 (2024)
   PUBMED   38763404
  REMARK    GeneRIF: Bag1 protein loss sensitizes mouse embryonic fibroblasts
            to glutathione depletion.
REFERENCE   3  (bases 1 to 1365)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1365)
  AUTHORS   Soma,S., Murakami,K., Fukuchi,Y., Kunimoto,H. and Nakajima,H.
  TITLE     O-Linked N-Acetylglucosamine Transferase Ensures Survival of Mouse
            Fetal Liver Hematopoietic Progenitors Partly by Regulating Bcl-xL
            and Oxidative Phosphorylation
  JOURNAL   Stem Cells 42 (1), 55-63 (2024)
   PUBMED   37813816
REFERENCE   5  (bases 1 to 1365)
  AUTHORS   Stuart,J.K., Myszka,D.G., Joss,L., Mitchell,R.S., McDonald,S.M.,
            Xie,Z., Takayama,S., Reed,J.C. and Ely,K.R.
  TITLE     Characterization of interactions between the anti-apoptotic protein
            BAG-1 and Hsc70 molecular chaperones
  JOURNAL   J Biol Chem 273 (35), 22506-22514 (1998)
   PUBMED   9712876
REFERENCE   6  (bases 1 to 1365)
  AUTHORS   Takayama,S., Krajewski,S., Krajewska,M., Kitada,S., Zapata,J.M.,
            Kochel,K., Knee,D., Scudiero,D., Tudor,G., Miller,G.J.,
            Miyashita,T., Yamada,M. and Reed,J.C.
  TITLE     Expression and location of Hsp70/Hsc-binding anti-apoptotic protein
            BAG-1 and its variants in normal tissues and tumor cell lines
  JOURNAL   Cancer Res 58 (14), 3116-3131 (1998)
   PUBMED   9679980
  REMARK    GeneRIF: Alternate protein isoforms arise through the use of a
            non-AUG (CUG) codon in addition to two downstream in-frame AUG
            codons.
REFERENCE   7  (bases 1 to 1365)
  AUTHORS   Packham,G., Brimmell,M. and Cleveland,J.L.
  TITLE     Mammalian cells express two differently localized Bag-1 isoforms
            generated by alternative translation initiation
  JOURNAL   Biochem J 328 (Pt 3) (Pt 3), 807-813 (1997)
   PUBMED   9396724
  REMARK    GeneRIF: The longer p50 isoform is generated through use of an
            upstream, in-frame, non-AUG translation initiation codon.
REFERENCE   8  (bases 1 to 1365)
  AUTHORS   Takayama,S., Bimston,D.N., Matsuzawa,S., Freeman,B.C.,
            Aime-Sempe,C., Xie,Z., Morimoto,R.I. and Reed,J.C.
  TITLE     BAG-1 modulates the chaperone activity of Hsp70/Hsc70
  JOURNAL   EMBO J 16 (16), 4887-4896 (1997)
   PUBMED   9305631
REFERENCE   9  (bases 1 to 1365)
  AUTHORS   Takayama,S., Kochel,K., Irie,S., Inazawa,J., Abe,T., Sato,T.,
            Druck,T., Huebner,K. and Reed,J.C.
  TITLE     Cloning of cDNAs encoding the human BAG1 protein and localization
            of the human BAG1 gene to chromosome 9p12
  JOURNAL   Genomics 35 (3), 494-498 (1996)
   PUBMED   8812483
REFERENCE   10 (bases 1 to 1365)
  AUTHORS   Takayama,S., Sato,T., Krajewski,S., Kochel,K., Irie,S., Millan,J.A.
            and Reed,J.C.
  TITLE     Cloning and functional analysis of BAG-1: a novel Bcl-2-binding
            protein with anti-cell death activity
  JOURNAL   Cell 80 (2), 279-284 (1995)
   PUBMED   7834747
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CX225744.1, AK009149.1,
            AK013611.1 and BQ031093.1.
            
            Summary: The oncogene Bcl2 encodes a membrane protein that blocks a
            step in a pathway leading to apoptosis or programmed cell death.
            The protein encoded by this gene binds to Bcl2 protein and is
            referred to as Bcl2-associated athanogene. It enhances the
            anti-apoptotic effects of Bcl2 and represents a link between growth
            factor receptors and anti-apoptotic mechanisms. At least two
            protein isoforms are encoded by this mRNA through the use of a
            non-AUG (CUG) start site and an alternative, downstream, AUG
            translation initiation site. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This transcript (1) encodes multiple isoforms
            due to the use of alternative translation initiation codons. The
            longer isoform (BAG-1L or p50) is derived from an upstream non-AUG
            (CUG) start codon, while the shorter isoform (BAG-1S or p32) is
            derived from a downstream AUG start codon. The shorter isoform (1S)
            is represented in this RefSeq.
            
            CCDS Note: This CCDS ID represents the shortest mouse Bag1 isoform,
            known as BAG-1S or p32, as described in the literature, including
            PMIDs 9679980 and 9396724. This isoform initiates translation at an
            AUG start codon. Alternative translation initiation at an upstream
            non-AUG (CUG) start codon produces an isoform that is 136 aa longer
            at the N-terminus, known as BAG-1L or p50. The longer isoform is
            represented by CCDS 38713.1. Evidence in PMID:9396724 indicates
            that these isoforms may have distinct subcellular distributions,
            which may contribute to the multifunctionality of the protein.
            Alternative translation initiation resulting in isoforms with
            differential subcellular localization has also been demonstrated
            for the human ortholog.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003722.1, AK154469.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-364               CX225744.1         1-364
            365-1196            AK009149.1         276-1107
            1197-1218           AK013611.1         483-504
            1219-1365           BQ031093.1         1-147               c
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 20.67 cM"
     gene            1..1365
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /note="BCL2-associated athanogene 1"
                     /db_xref="GeneID:12017"
                     /db_xref="MGI:MGI:108047"
     exon            1..573
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /inference="alignment:Splign:2.1.0"
     CDS             501..1160
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /note="isoform 1S is encoded by transcript variant 1;
                     Bcl-2-binding protein; BAG family molecular chaperone
                     regulator 1; bcl-2-associated athanogene 1"
                     /codon_start=1
                     /product="BAG family molecular chaperone regulator 1
                     isoform 1S"
                     /protein_id="NP_001165210.1"
                     /db_xref="CCDS:CCDS51139.1"
                     /db_xref="GeneID:12017"
                     /db_xref="MGI:MGI:108047"
                     /translation="MAKTEEMVQTEEMETPRLSVIVTHSNERYDLLVTPQQGNSEPVV
                     QDLAQLVEEATGVPLPFQKLIFKGKSLKEMETPLSALGMQNGCRVMLIGEKSNPEEEV
                     ELKKLKDLEVSAEKIANHLQELNKELSGIQQGFLAKELQAEALCKLDRKVKATIEQFM
                     KILEEIDTMVLPEQFKDSRLKRKNLVKKVQVFLAECDTVEQYICQETERLQSTNLALA
                     E"
     exon            574..702
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /inference="alignment:Splign:2.1.0"
     exon            703..785
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /inference="alignment:Splign:2.1.0"
     exon            786..899
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1007
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1070
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1349
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1301..1306
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /note="hexamer: AATGAA"
     polyA_site      1330
                     /gene="Bag1"
                     /gene_synonym="BAG-1; Rap46"
                     /note="major polyA site"
ORIGIN      
        1 ggcgagtaga tggggcggaa ctggtgagta gtggggcggg gttgcaaggc tgcgcgcccg
       61 gtagccaccg ctgcgctgtg aacaaacgcg gcctggcggg tcgcagcgcc gcgcggcgac
      121 cgcgaggcga ccgggagccg ctgggaccca ggctgcgcgc ccctcggcct gcccgggagc
      181 cgcgccagtc ggagtcccgg gccgagcggg gcttgcctcc ctctcagcgt tcctctgtgc
      241 gcagtgcagc cagcgggcat gaccgatcca ccaggggcgc cgccgccggc gcctgcaagc
      301 cgcgggtgaa gaagaaagtc cggccccgct cttctcagag cgagaaggta gggagcagca
      361 gcagggagtt gactagaagt aagaaagtga cccgtagcaa gaacgtgacc gggacccagg
      421 tagaggaggt gaccaagatc gaggaggcga cccaaaccga ggaagtaact gtggcagaag
      481 aggtgaccca gaccgacaac atggccaaga ccgaggagat ggtccagacg gaggaaatgg
      541 aaacacccag actcagcgtg atcgtcaccc acagcaatga gaggtatgac cttcttgtta
      601 ccccacagca aggtaacagt gagccagttg tccaagactt ggctcagctt gttgaagagg
      661 ccacaggagt tccactacct tttcagaagc tcatatttaa gggaaaatct ctgaaagaaa
      721 tggaaacacc gttgtcagca cttggaatgc aaaatggttg ccgagtcatg ttaattggtg
      781 aaaagagcaa tccagaagaa gaggttgagt taaagaagct gaaagatttg gaggtatctg
      841 cagagaagat agctaaccac ctgcaagaat tgaataaaga gctttctggc atccagcagg
      901 gttttctggc taaggaattg caagcggagg ctctctgcaa acttgatagg aaagtaaaag
      961 caacaattga gcaattcatg aagatcttgg aggagattga cacaatggtc ctaccagaac
     1021 aatttaaaga cagcaggcta aaaaggaaga atttggtgaa aaaggttcag gtgttcttag
     1081 cagagtgtga cacagtggag caatacatct gccaagagac agagcggctg cagtctacaa
     1141 acttggccct ggctgaatga agtgcagtgg agagtggctg tactggcctg aagagcagct
     1201 ttacagccct gccctctctg gaacagaagt cgcctgtttc tccatggctg ccaggggcaa
     1261 ctagccaaat gtcaatttcc ctgctcctcc gtcggttctc aatgaaaaag tcctgtcttt
     1321 gcaacctgaa ttagacttgt gttttctcaa aaaaaaaaaa aaaaa
//
