LOCUS       NM_008081               4086 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus beta-1,4-N-acetyl-galactosaminyl transferase 2
            (B4galnt2), mRNA.
ACCESSION   NM_008081
VERSION     NM_008081.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Mamsa,H., Stark,R.L., Shin,K.M., Beedle,A.M. and Crosbie,R.H.
  TITLE     Sarcospan increases laminin-binding capacity of alpha-dystroglycan
            to ameliorate DMD independent of Galgt2
  JOURNAL   Hum Mol Genet 31 (5), 718-732 (2022)
   PUBMED   34581784
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Cramer,M.L., Xu,R. and Martin,P.T.
  TITLE     Soluble Heparin Binding Epidermal Growth Factor-Like Growth Factor
            Is a Regulator of GALGT2 Expression and GALGT2-Dependent Muscle and
            Neuromuscular Phenotypes
  JOURNAL   Mol Cell Biol 39 (14), e00140-19 (2019)
   PUBMED   31036568
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4086)
  AUTHORS   Vallier,M., Abou Chakra,M., Hindersin,L., Linnenbrink,M.,
            Traulsen,A. and Baines,J.F.
  TITLE     Evaluating the maintenance of disease-associated variation at the
            blood group-related gene B4galnt2 in house mice
  JOURNAL   BMC Evol Biol 17 (1), 187 (2017)
   PUBMED   28806915
  REMARK    GeneRIF: B4galnt2 displays an interesting spatial allelic
            distribution in Western Europe, likely due to the recent action of
            natural selection.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4086)
  AUTHORS   Thomas,P.J., Xu,R. and Martin,P.T.
  TITLE     B4GALNT2 (GALGT2) Gene Therapy Reduces Skeletal Muscle Pathology in
            the FKRP P448L Mouse Model of Limb Girdle Muscular Dystrophy 2I
  JOURNAL   Am J Pathol 186 (9), 2429-2448 (2016)
   PUBMED   27561302
  REMARK    Erratum:[Am J Pathol. 2016 Dec;186(12):3316. doi:
            10.1016/j.ajpath.2016.10.001.. PMID: 27876153]
REFERENCE   5  (bases 1 to 4086)
  AUTHORS   Xu,R., Singhal,N., Serinagaoglu,Y., Chandrasekharan,K., Joshi,M.,
            Bauer,J.A., Janssen,P.M. and Martin,P.T.
  TITLE     Deletion of Galgt2 (B4Galnt2) reduces muscle growth in response to
            acute injury and increases muscle inflammation and pathology in
            dystrophin-deficient mice
  JOURNAL   Am J Pathol 185 (10), 2668-2684 (2015)
   PUBMED   26435413
  REMARK    GeneRIF: Overexpression of Galgt2 failed to inhibit skeletal muscle
            pathology in dystroglycan-deficient muscles
REFERENCE   6  (bases 1 to 4086)
  AUTHORS   Wilson,S.D., Billings,P.R., D'Eustachio,P., Fournier,R.E.,
            Geissler,E., Lalley,P.A., Burd,P.R., Housman,D.E., Taylor,B.A. and
            Dorf,M.E.
  TITLE     Clustering of cytokine genes on mouse chromosome 11
  JOURNAL   J Exp Med 171 (4), 1301-1314 (1990)
   PUBMED   1969921
REFERENCE   7  (bases 1 to 4086)
  AUTHORS   Takahashi,M., Yoshida,M.C., Satoh,H., Hilgers,J., Yaoita,Y. and
            Honjo,T.
  TITLE     Chromosomal mapping of the mouse IL-4 and human IL-5 genes
  JOURNAL   Genomics 4 (1), 47-52 (1989)
   PUBMED   2563351
REFERENCE   8  (bases 1 to 4086)
  AUTHORS   Bernier,L., Colman,D.R. and D'Eustachio,P.
  TITLE     Chromosomal locations of genes encoding 2',3' cyclic nucleotide
            3'-phosphodiesterase and glial fibrillary acidic protein in the
            mouse
  JOURNAL   J Neurosci Res 20 (4), 497-504 (1988)
   PUBMED   2903254
REFERENCE   9  (bases 1 to 4086)
  AUTHORS   Ozawa,M., Huang,R.P., Furukawa,T. and Muramatsu,T.
  TITLE     A teratocarcinoma glycoprotein carrying a developmentally regulated
            carbohydrate marker is a member of the immunoglobulin gene
            superfamily
  JOURNAL   J Biol Chem 263 (7), 3059-3062 (1988)
   PUBMED   2963822
REFERENCE   10 (bases 1 to 4086)
  AUTHORS   Mock,B., Hilgers,J., Huppi,K. and D'Hoostelaere,L.
  TITLE     A TaqI restriction fragment length polymorphism at the Hox-2.1
            locus cosegregates with the Dlb-1 locus on mouse chromosome 11
  JOURNAL   Nucleic Acids Res 15 (5), 2397 (1987)
   PUBMED   2882472
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL606704.20 and AL593858.20.
            
            On Feb 7, 2007 this sequence version replaced NM_008081.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L30104.1, BC139165.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                AL606704.20        8209-8260           c
            53-268              AL593858.20        183445-183660       c
            269-406             AL593858.20        182660-182797       c
            407-513             AL593858.20        180494-180600       c
            514-551             AL593858.20        175630-175667       c
            552-732             AL593858.20        167785-167965       c
            733-819             AL593858.20        165549-165635       c
            820-1007            AL593858.20        160921-161108       c
            1008-1148           AL593858.20        160037-160177       c
            1149-1368           AL593858.20        158437-158656       c
            1369-4086           AL593858.20        155246-157963       c
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 59.02 cM"
     gene            1..4086
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /note="beta-1,4-N-acetyl-galactosaminyl transferase 2"
                     /db_xref="GeneID:14422"
                     /db_xref="MGI:MGI:1342058"
     exon            1..52
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1574
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /EC_number="2.4.1.-"
                     /note="N-acetylgalactosaminyltransferase;
                     UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-
                     galactosyl-N-acetylglucosaminylpolypeptide-beta-1,
                     4-N-acetylgalactosaminyltransferase; dolichos lectin
                     binding 1; CT-Gal NAc transferase"
                     /codon_start=1
                     /product="beta-1,4 N-acetylgalactosaminyltransferase 2"
                     /protein_id="NP_032107.1"
                     /db_xref="CCDS:CCDS25285.1"
                     /db_xref="GeneID:14422"
                     /db_xref="MGI:MGI:1342058"
                     /translation="MTSSVSFASFRFPWLLKTFVLMVGLATVAFMVRKVSLTTDFSTF
                     KPKFPEPARVDPVLKLLPEEHLRKLFTYSDIWLFPKNQCDCNSGKLRMKYKFQDAYNQ
                     KDLPAVNARRQAEFEHFQRREGLPRPPPLLAPPNLPFGYPVHGVEVMPLHTILIPGLQ
                     YEGPDAPVYEVILKASLGTLNTLADVPDDEVQGRGQRQLTISTRHRKVLNFILQHVTY
                     TSTEYYLHKVDTVSMEYESSVAKFPVTIKQQTVPKLYDPGPERKIRNLVTIATKTFLR
                     PHKLKILLQSIRKYYPDITVIVADDSKEPLEINDDYVEYYTMPFGKGWFAGRNLAISQ
                     VTTKYVLWVDDDFLFSDKTKIEVLVDVLEKTELDVVGGSVQGNTYQFRLLYEQTKNGS
                     CLHQRWGSFQALDGFPGCTLTSGVVNFFLAHTEQLRRVGFDPILQRVAHGEFFIDGLG
                     RLLVGSCPGVIINHQVRTPPKDPKLAALEKTYDKYRANTNSVIQFKVALQYFKNHLYC
                     ST"
     exon            53..268
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            269..406
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            407..513
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            514..551
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            552..732
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            733..819
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            820..1007
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1148
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1368
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..4086
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4058..4063
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /note="hexamer: AATAAA"
     polyA_site      4079
                     /gene="B4galnt2"
                     /gene_synonym="Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1"
                     /note="major polyA site"
ORIGIN      
        1 ctgcggtagg cagtctgcag aagtggctgg agcgagctgc gatgacttcc agcgtgtctt
       61 ttgccagctt caggtttcca tggctcctca agacatttgt cctcatggta ggacttgcca
      121 ctgttgcgtt tatggtgaga aaggtgtccc ttacaacaga cttcagcacc ttcaagccaa
      181 agtttccaga gcctgcaagg gttgacccag tgctgaagct tctaccagag gagcatctga
      241 ggaaactctt tacctacagt gacatctggc tcttccccaa aaatcagtgt gactgtaact
      301 ctggcaaact gcgaatgaaa tataagtttc aggatgccta taaccagaag gaccttccag
      361 ctgtgaatgc cagaagacag gcagaatttg agcactttca gaggagagaa gggctgcctc
      421 gcccaccacc tctgctggct ccacccaatc tccccttcgg atacccagtc catggtgtgg
      481 aggtgatgcc cctgcataca attctcatcc caggcctcca gtatgaaggg ccagatgctc
      541 cagtctatga ggtcatcctg aaagcttctc tggggacact gaacaccctt gctgatgtgc
      601 cagatgatga ggttcagggc agaggccaga ggcagctgac catttccacc agacatcgga
      661 aggtcctgaa tttcatcctt cagcatgtga cgtacaccag cacagagtac tatctccaca
      721 aggtggacac agtaagtatg gaatacgagt cgtcagtggc caagtttcca gtgactatca
      781 aacaacagac tgtacccaag ttgtatgacc ctggacctga gaggaagatc agaaacctgg
      841 tgaccattgc cacgaagact tttctccgtc cccacaagct taagatcctg cttcagagta
      901 ttcgaaaata ttacccagac atcaccgtga ttgtagctga tgacagcaag gagcccctgg
      961 aaattaatga tgactacgtg gagtactaca ccatgccctt tgggaagggc tggtttgctg
     1021 ggaggaacct ggccatctca caggtgacta ctaaatatgt cctctgggtg gacgatgact
     1081 ttctcttcag cgacaagacc aagattgagg tactggtgga tgtcctggag aaaaccgaac
     1141 tggatgtggt gggtggcagc gtgcagggga atacttacca gttcaggctg ctgtatgaac
     1201 agaccaagaa tgggagctgt cttcaccaga ggtggggatc attccaggcc cttgacggct
     1261 ttcccggatg cacgttgacc agcggcgtgg tgaacttctt cttggctcac acggaacaac
     1321 tccgaagagt tggttttgat cccatcttgc aacgagtggc ccacggagag ttctttattg
     1381 atgggctggg gagactgttg gtgggctctt gcccgggtgt aattataaac caccaagtaa
     1441 gaacaccacc aaaggatcca aagctggctg ccttggagaa gacttatgac aaataccggg
     1501 ccaacaccaa ttctgtgatc caattcaagg ttgcactcca gtacttcaag aaccatctct
     1561 actgctccac ttaaaaagta ccaagaccag gaatcgcaat aaacagatta gcggcgggca
     1621 acaaaaaaaa tagtaggatt ggacattgga gggtaccaaa agcagactcc taacaagggg
     1681 aacagggaac aaagaagctg gaagctggag ctagggtagg accctgaagt cggctcagga
     1741 cccccaagac caaattctca gcacagagat ttatctgccc tagagagaga aagggcaggg
     1801 aatgagacaa agacaagggc tggaaggtgg tggggcacac ctttaatcac agcactccgg
     1861 gggcagaagc aggtggatct ctgcgcattt tgaggacagc ctggtctaca gagagagttc
     1921 caggacagcc agggctacag agaaactctt atcttaaaaa ggcaataagt tgatctaaaa
     1981 taatgttatt taaagccaga tgataaggtt ggtgtagtgt tgtgatccca aagcccagta
     2041 actgaagtag gaggattgag ttcaaggcca gcctgggcta tgtaacagcc catctttaaa
     2101 tgagtgacta gggggctgga gagagctcag tgcttaagag cgattaggac agaggaccta
     2161 gattggctcc caggacccac atgatgagtc acaacttcat acctgcagtt tcagggaatt
     2221 ggaagcatct gcttacctcc aaaggctcct gcatatgagt ggtgcataca tacacgcagg
     2281 cacacacata tgcacacaaa atacgcttta aaataaatga aggaatgagt gaatggctaa
     2341 atattaacca aataattccg attttatttt gtcaagactg cctggcctag cactcactgt
     2401 gtaagactga cctcacactc atggcaatcc tcctgtctcc acttcccagt gctgaggtat
     2461 gaggtgtgaa ccacaatttc tggcttggat ttttaaaaag aatttacaca cacacacata
     2521 catacacaca cactgtagct gtcttcagac acatcagaaa gggcatcaga tcccattaca
     2581 gatggttgtg agccaccatg tggttgctgg gaattgaact cagaacctct ggaaaagcag
     2641 tcagtgctct taactgataa gccatctctc tagtctctag cttgattttt aaactttgac
     2701 ttttaaatat acttattcat tataattttt tttctttttg agacagggtt tctctgtgta
     2761 gccctggctg tcctggaact cactctgtag accaggctgg cctcgaactc agaaatccgc
     2821 ctgcctctgc ctcccgaggg ctgggattaa aggcatgcac caccatgccc ggcttatatt
     2881 ttgattttaa tgggatatta agatggatgt taagattgtg agctttcaac cctgtctcgg
     2941 aaaaccaaaa aaaaaaaaaa aaagaaagaa aagattgtga gccttcatgt ggttgttggg
     3001 gatagaatct ccactcactc cagtcaaccc tgttctcagt ccctgctaac tccggcccaa
     3061 agatttattt attattataa ttattatacg taagtacact gtagctgact tcagacacac
     3121 tagaagagtg tcagatctca ttaccggtag ttgagtcacc atgtagttgc ggggacttga
     3181 actcaggatc tttggaagag cagtccatcc tcttaccctc tgagccatct cgccagccca
     3241 tttcaacact ctgaacatac ttacacttta ttttttactt cctgaggtaa atattgctct
     3301 gagctagacc atagaaagcc aaacaccagt gggtaaggag gcgaggaatg acatgccagc
     3361 tagttgtggt gtgggccttt aatcccagcc ttgaattcag agatcctcta gccagggcta
     3421 ggcagtgagc tctgttttgt tttgttttgt tctgttttca gaacaaaacc aaaatcaggg
     3481 gctgaggaaa tggctcaaaa ggtgaagtgt ttgttaccca agcatgagga cttgagttca
     3541 gattcccaga agtaaggcat agctgccagt ctgtaaccct gggcattctc acagcaaatg
     3601 ttaacatcct ccattttgac tgggttgtcg ttagcatgct caaacacagt cttttctcta
     3661 cttcaagatc tagaaccatc tctccagaag agagagagag agacagagag tgagttagtt
     3721 tcctatgatc cagtaacacc tcttccccag gaacacagga tgttctaaaa ggacatccat
     3781 tcaactcaaa gggttcaggg actccctgaa acccaccaga cagactaggc cccctctttt
     3841 ccaagtatat tagctgtgaa gactgctgag aggcactctc agacaagttg agctgcctga
     3901 aacagacatt ctctaacctg ttgagctgtc tgcaagttgt acagagggct tcaagtttac
     3961 agcttttgtg acatggtgag ctttcatggt gccgctgtct ttcgagtcat ttgtgatcct
     4021 gcggtaaccc ctcacccata cttctgtctg taaccccaat aaaactcatc agtctgctat
     4081 actgga
//
