LOCUS       NM_054052               4548 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus UDP-GlcNAc:betaGal
            beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript
            variant 2, mRNA.
ACCESSION   NM_054052
VERSION     NM_054052.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Kuan,C.T., Chang,J., Mansson,J.E., Li,J., Pegram,C., Fredman,P.,
            McLendon,R.E. and Bigner,D.D.
  TITLE     Multiple phenotypic changes in mice after knockout of the B3gnt5
            gene, encoding Lc3 synthase--a key enzyme in lacto-neolacto
            ganglioside synthesis
  JOURNAL   BMC Dev Biol 10, 114 (2010)
   PUBMED   21087515
  REMARK    GeneRIF: Results demonstrate that Lc3 synthase and lacto-neolacto
            series gangliosides are essential to multiple physiological
            functions, especially the control of reproductive output, and
            spleen B-cell abnormality.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Togayachi,A., Kozono,Y., Ikehara,Y., Ito,H., Suzuki,N., Tsunoda,Y.,
            Abe,S., Sato,T., Nakamura,K., Suzuki,M., Goda,H.M., Ito,M.,
            Kudo,T., Takahashi,S. and Narimatsu,H.
  TITLE     Lack of lacto/neolacto-glycolipids enhances the formation of
            glycolipid-enriched microdomains, facilitating B cell activation
  JOURNAL   Proc Natl Acad Sci U S A 107 (26), 11900-11905 (2010)
   PUBMED   20547865
REFERENCE   3  (bases 1 to 4548)
  AUTHORS   Biellmann,F., Hulsmeier,A.J., Zhou,D., Cinelli,P. and Hennet,T.
  TITLE     The Lc3-synthase gene B3gnt5 is essential to pre-implantation
            development of the murine embryo
  JOURNAL   BMC Dev Biol 8, 109 (2008)
   PUBMED   19014510
  REMARK    GeneRIF: B3gnt5 is essential to pre-implantation development of the
            murine embryo.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4548)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   5  (bases 1 to 4548)
  AUTHORS   Henion,T.R., Zhou,D., Wolfer,D.P., Jungalwala,F.B. and Hennet,T.
  TITLE     Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase
            GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding
            the key regulator of lacto-series glycolipid biosynthesis
  JOURNAL   J Biol Chem 276 (32), 30261-30269 (2001)
   PUBMED   11384981
REFERENCE   6  (bases 1 to 4548)
  AUTHORS   Togayachi,A., Akashima,T., Ookubo,R., Kudo,T., Nishihara,S.,
            Iwasaki,H., Natsume,A., Mio,H., Inokuchi,J., Irimura,T., Sasaki,K.
            and Narimatsu,H.
  TITLE     Molecular cloning and characterization of
            UDP-GlcNAc:lactosylceramide beta
            1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential
            enzyme for the expression of HNK-1 and Lewis X epitopes on
            glycolipids
  JOURNAL   J Biol Chem 276 (25), 22032-22040 (2001)
   PUBMED   11283017
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK083839.1, BC063076.1 and BB667148.1.
            
            On Apr 16, 2009 this sequence version replaced NM_054052.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. All three variants encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083839.1, BC063076.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2208              AK083839.1         1-2208
            2209-4258           BC063076.1         2044-4093
            4259-4548           BB667148.1         364-653
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 12.23 cM"
     gene            1..4548
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /note="UDP-GlcNAc:betaGal
                     beta-1,3-N-acetylglucosaminyltransferase 5"
                     /db_xref="GeneID:108105"
                     /db_xref="MGI:MGI:2137302"
     exon            1..244
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /inference="alignment:Splign:2.1.0"
     exon            245..431
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /inference="alignment:Splign:2.1.0"
     exon            432..538
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /inference="alignment:Splign:2.1.0"
     exon            539..4548
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /inference="alignment:Splign:2.1.0"
     CDS             828..1958
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /EC_number="2.4.1.206"
                     /note="BGnT-5; beta3Gn-T5; lc3 synthase; beta-1,3-Gn-T5;
                     lc(3)Cer synthase; lactotriaosylceramide synthase;
                     beta-1,3-N-acetylglucosaminyltransferase 5;
                     UDP-GlcNAc:beta-Gal
                     beta-1,3-N-acetylglucosaminyltransferase 5"
                     /codon_start=1
                     /product="lactosylceramide
                     1,3-N-acetyl-beta-D-glucosaminyltransferase"
                     /protein_id="NP_473393.2"
                     /db_xref="CCDS:CCDS28040.1"
                     /db_xref="GeneID:108105"
                     /db_xref="MGI:MGI:2137302"
                     /translation="MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSY
                     SYRYLVNSYGFVNNSLSLKHSSVQPHYPYLINHREKCQAQDVLLLLFIKTAPENYGRR
                     SAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKELQKRLIGEDQVYKDIIQQDFI
                     DSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQIGVRDFWI
                     GHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSS
                     MYIDDVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEA
                     THPKVKNISKGFFGQIYCRLIKIVLLCRLTYRNSYPCWAAFA"
     regulatory      4205..4210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /note="hexamer: AATAAT"
     regulatory      4239..4244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /note="hexamer: AATAAA"
     polyA_site      4249
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
     polyA_site      4280
                     /gene="B3gnt5"
                     /gene_synonym="beta3GnT5"
                     /note="major polyA site"
ORIGIN      
        1 gaccaaataa gcagctggag gctcctgcgg ctgcgcccca gcctgagtga gccaagaccc
       61 ttaatcaggc ccgcccctct cagccggctc tagccggctc cagcggggtc tctcttggag
      121 ccctgcccta gcttcctcgc ttacaaccga aatttagttt gcaacttttg ttcttgagac
      181 ttttccccca gggcttctag gaggacagcg gacctctggg acatttaagg acttgggttc
      241 ctcggctccc aggagctcaa gtcctaagtg tcctgacaga agaccggcgg gctcttaggg
      301 tttgctagga aactacgaga cgatgggaga accgctgtcc actcaaaacc ttcagctcgg
      361 aaatgactaa ctgaaacgtg gtttggatga atgggtctcc agtcttaacc aacgccgctc
      421 ctggatgaaa ggtcgagatt tcaggttgca acattatacc tactgattct ttggtggact
      481 atcaacccct gaaaggtcaa tgaagtcaca tctagcatcc tgtctgtggc cttttcaggg
      541 ctacagtgca tgttcgtaga aagaactacg tggatcaagc atgtgaggcc ataagcagta
      601 cttactgagg aacaccaggg tcgacggatt tggatgtacc aatgtcttca ttctatagtg
      661 tcccatcctt ttaccatccc agagaaaatg ttgctagaag agaatattgt gtggaatgag
      721 accttttccc atattttatg agcaatatgc attaagaaat gcatctattc tacagagctc
      781 tatagccctg aagaagcaag cagccatata cccacagacc agagaacatg agactgtttg
      841 ttagcagaag agtcaaaaga tggaaaattt ttcacttttt tgtcacttgt tttatattaa
      901 gcttcatggt tttttggagc ccaatcaata attacatcat gagccatatg aagtcctact
      961 cctacagata cctcgtaaat agctatggct ttgtaaacaa ttccctgtct ctcaagcaca
     1021 gctctgtgca gcctcactac ccatacttga tcaaccacag agagaagtgt caggctcaag
     1081 atgtcctcct cttactgttt ataaagactg cccctgaaaa ctatggccga cgttctgcaa
     1141 tcagaaaaac gtggggcaat gagaactatg ttcagtctca actcaatgcc aacatcaaaa
     1201 ttctgtttgc attaggaact cctggtccac tgaagggaaa agaactgcaa aaaagactaa
     1261 tcggggaaga tcaagtgtac aaggatataa ttcagcaaga tttcattgat tctttccaca
     1321 atcttacttc taaattcctt cttcagttca gctgggcaaa taccttttgt ccacatgcca
     1381 aattcctgat gactgctgat gatgatatat ttatccacat gccaaatctc attgaatatc
     1441 ttcaagggct agagcagatt ggagttcgag acttttggat tggtcacgtt catcgaggtg
     1501 gccctcctgt tagggataaa agcagcaaat actatgttcc ctatgaaatg tacaagtggc
     1561 cagcctaccc tgactataca gctggtgctg cctatgttgt ctccagagat gtagctgcca
     1621 aaatctatga ggcatcgcag acactgaatt ccagtatgta catagatgat gtattcatgg
     1681 gcctctgtgc caataaagtg ggaatcttgc cacaggacca tgtatttttc tctggggaag
     1741 ggaaaattcc ttatcacccc tgcatctatg aaaagatgat gacatctcac ggacacttac
     1801 aagatctgca ggacctctgg atagaggcta cacatcctaa agtaaagaac atttcaaaag
     1861 ggttttttgg tcaaatatac tgcaggttaa ttaagatagt tcttctctgc agactgactt
     1921 acaggaattc atacccttgt tgggctgcat ttgcttagta gtacatgaat gctgtactgt
     1981 gttaagtgtc actgagccaa acttgaatgg aaaataataa tctgtaaatg tttctctcta
     2041 ccctaagtca aatgaattga aagcaaagag attcatttca aaacccagtc aaactggtca
     2101 gtgtaactcc aaattaaact gcagcttaat ttcatttcaa ggaattcata ttctaaacga
     2161 cttagatcat gagcaagggt aaagtaagaa tttcaagttc tcatttggtt ctacacatgt
     2221 atttaaggta tggagaacac ctgagaagcc ttgatatcaa aataatagtt tggggaagat
     2281 acctgagaaa tatataatat agcatttcta aaaaattaat acctcttaga acaagcaatc
     2341 ccaattatct tattaaagag ttctaggtag aggtggaggg aggcaggtga tgcagaagga
     2401 acgtgatgaa aggttcatcc gaatgaggac atatgtggaa aatgtatata atttcttata
     2461 gttaagtcgt ttaacattgg cattcctaca gccagttacc taggcagttc tggaatcatt
     2521 taagatatat ttgtttcatt ttctcaaatt taatttttag tgtaaaaact ttagaaagtg
     2581 gtcagctcta ccctaataaa aactatatgt acgctttctc taaagttata aaacaagaat
     2641 agttcctggc ccctggagga ccttctttaa agataaatca ctatcaactc caataagctt
     2701 gttttaattt aatgaattat tcaatggctt ttaaaacact agatattggc ctctgtttaa
     2761 ggtagttgtt tggatgcagc ttacaatgag gagtataata acagaagaga cttaaaggaa
     2821 agatagaact aggggtatag ctcagcatcc aaaagggaag acaaagaagc aataaaagtc
     2881 tcttttcctt gtaattgtaa catcagcttg gagtccagac catggcactc aatactcaaa
     2941 tgccgcttcc aataaggtct tgtaggacac agattttgtt ccaagtgaag acttttagct
     3001 tgaaagtttt taatatagta ccacgtctaa aataatccat tatttgactc aatagctatc
     3061 agaggttatc ttttaaatat ttcaaacatg ggagcaaaat aagagtgcta gagaaactga
     3121 ccaaatgaga ttagaaatgt tggtaaatct gcatgttctt tgtaatcaga ggcttggcag
     3181 atctaataaa ttaaattaca tttattttac aaaataggat taaaacatgt ggctggtatg
     3241 caaacagtgc accagcacag aaaggactaa ggggtattca tcgattaagg acctgtaatg
     3301 caggtcaaaa cacgatttca gcaaatgaag atggatgctt aacaaccctc agacagatgc
     3361 atataatagg gaaagtttag ctactattat tagggtggtt ttggaaacaa tgcaaaaaaa
     3421 tctaaaaata cacaactaaa tgatatctgt gttttcacag gtagattttt tttaaaaaaa
     3481 tgtttacaga aatctttgtg aagtttttta ttatgagata tgaagcttgt ttatcctact
     3541 cgggtagttt cctactaatt tcaagactag tgttaaacat tctaaggtag ttatcttagg
     3601 gtagattcaa ggttttagat gactaacagt tcagattttc tgatcaattt tttaaacact
     3661 agagaataaa agtgtactag agaataaaag cagcttcata gttaattctc accaattggc
     3721 cctttgctag ctgctggctt taggtacaca taggataata tgtgtccacg tttctacttg
     3781 gaactggtaa aagttgtcac tggctggaaa atggtatctc tctcttgtat acaagatggt
     3841 ccattgacac tggtacttta tgaagcagtt ctttgtttgt ttgattgagc tctcttgaac
     3901 cttgttcatc ttttagtttt tgcttagaat agaatggaac tggtttgaag ttaaaggaaa
     3961 tattcatttt gaaacttgtt cattttgaaa ggaaatgcaa gtttcaaaat gaaaaataaa
     4021 atgaaaaagg aaataaatta ttgtcccaga tggtcacttg agttttaaaa aatggctgca
     4081 cacagtaaaa ctgctaaaaa caaaaactta cctcattatt ggtttgcatc ttttttcagc
     4141 tactaatttt ataccaaaat gttaaatatt tatattgttt gagtttcaat cttgtatgga
     4201 aaaaaataat tagtaggtct aaaaatgcca tgctttccaa taaagaagtt aaaaaaatca
     4261 tcagtaatgt gaatttaaaa tcctgtgtgg gcatcattct gtgtatcgtc acctgctgtc
     4321 ttataccaga agaacctgca ctacccatcc attgtcctat gtgtgctcac cctgctgctg
     4381 tgctcactgg tgggctgtca tggagctact taaggagcta tgctgtgact gtgggaaatg
     4441 ctgtttgtcc agaatgccat gacaaaaagc tcttgcagtt ttagtgtatt aagatgccat
     4501 ctaaagaggt tagactctat tcattagctg aataaaagaa gaacttgg
//
