LOCUS       NM_001081204            4570 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus beta-3-glucosyltransferase (B3glct), mRNA.
ACCESSION   NM_001081204 XM_355669
VERSION     NM_001081204.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Berardinelli,S.J., Sillato,A.R., Grady,R.C., Neupane,S., Ito,A.,
            Haltiwanger,R.S. and Holdener,B.C.
  TITLE     O-fucosylation of thrombospondin type I repeats is dispensable for
            trafficking thrombospondin 1 to platelet secretory granules
  JOURNAL   Glycobiology 33 (4), 301-310 (2023)
   PUBMED   36721988
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Liau,E.S., Jin,S., Chen,Y.C., Liu,W.S., Calon,M., Nedelec,S.,
            Nie,Q. and Chen,J.A.
  TITLE     Single-cell transcriptomic analysis reveals diversity within
            mammalian spinal motor neurons
  JOURNAL   Nat Commun 14 (1), 46 (2023)
   PUBMED   36596814
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4570)
  AUTHORS   Neupane,S., Goto,J., Berardinelli,S.J., Ito,A., Haltiwanger,R.S.
            and Holdener,B.C.
  TITLE     Hydrocephalus in mouse B3glct mutants is likely caused by defects
            in multiple B3GLCT substrates in ependymal cells and subcommissural
            organ
  JOURNAL   Glycobiology 31 (8), 988-1004 (2021)
   PUBMED   33909046
  REMARK    GeneRIF: Hydrocephalus in mouse B3glct mutants is likely caused by
            defects in multiple B3GLCT substrates in ependymal cells and
            subcommissural organ.
REFERENCE   4  (bases 1 to 4570)
  AUTHORS   Holdener,B.C., Percival,C.J., Grady,R.C., Cameron,D.C.,
            Berardinelli,S.J., Zhang,A., Neupane,S., Takeuchi,M.,
            Jimenez-Vega,J.C., Uddin,S.M.Z., Komatsu,D.E., Honkanen,R.,
            Dubail,J., Apte,S.S., Sato,T., Narimatsu,H., McClain,S.A. and
            Haltiwanger,R.S.
  TITLE     ADAMTS9 and ADAMTS20 are differentially affected by loss of B3GLCT
            in mouse model of Peters plus syndrome
  JOURNAL   Hum Mol Genet 28 (24), 4053-4066 (2019)
   PUBMED   31600785
  REMARK    GeneRIF: Results provide compelling evidence that ADAMTS9 and
            ADAMTS20 were differentially sensitive to B3GLCT inactivation and
            suggest that the developmental defects in PTRPLS result from
            disruption of a subset of highly sensitive POFUT2/B3GLCT targets
            such as ADAMTS20.
            Erratum:[Hum Mol Genet. 2020 Oct 10;29(17):2986-2987. doi:
            10.1093/hmg/ddaa090.. PMID: 32533185]
REFERENCE   5  (bases 1 to 4570)
  AUTHORS   Dubail,J., Vasudevan,D., Wang,L.W., Earp,S.E., Jenkins,M.W.,
            Haltiwanger,R.S. and Apte,S.S.
  TITLE     Impaired ADAMTS9 secretion: A potential mechanism for eye defects
            in Peters Plus Syndrome
  JOURNAL   Sci Rep 6, 33974 (2016)
   PUBMED   27687499
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4570)
  AUTHORS   Sato,T., Sato,M., Kiyohara,K., Sogabe,M., Shikanai,T., Kikuchi,N.,
            Togayachi,A., Ishida,H., Ito,H., Kameyama,A., Gotoh,M. and
            Narimatsu,H.
  TITLE     Molecular cloning and characterization of a novel human
            beta1,3-glucosyltransferase, which is localized at the endoplasmic
            reticulum and glucosylates O-linked fucosylglycan on thrombospondin
            type 1 repeat domain
  JOURNAL   Glycobiology 16 (12), 1194-1206 (2006)
   PUBMED   16899492
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114342.12.
            
            On May 19, 2018 this sequence version replaced NM_001081204.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927932.1927705.1,
                                           SRR9219379.42112.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-150               AC114342.12        112801-112950       c
            151-200             AC114342.12        94706-94755         c
            201-240             AC114342.12        87862-87901         c
            241-350             AC114342.12        81751-81860         c
            351-427             AC114342.12        65693-65769         c
            428-539             AC114342.12        64315-64426         c
            540-676             AC114342.12        61120-61256         c
            677-740             AC114342.12        58444-58507         c
            741-860             AC114342.12        55691-55810         c
            861-930             AC114342.12        51579-51648         c
            931-1044            AC114342.12        45641-45754         c
            1045-1144           AC114342.12        44576-44675         c
            1145-1264           AC114342.12        40630-40749         c
            1265-1409           AC114342.12        36971-37115         c
            1410-4570           AC114342.12        28581-31741         c
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 89.18 cM"
     gene            1..4570
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /note="beta-3-glucosyltransferase"
                     /db_xref="GeneID:381694"
                     /db_xref="MGI:MGI:2685903"
     exon            1..150
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..1577
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /EC_number="2.4.1.122"
                     /note="beta-1,3-glucosyltransferase;
                     beta-3-glycosyltransferase-like; beta
                     1,3-galactosyltransferase-like"
                     /codon_start=1
                     /product="beta-1,3-glucosyltransferase precursor"
                     /protein_id="NP_001074673.1"
                     /db_xref="CCDS:CCDS39410.1"
                     /db_xref="GeneID:381694"
                     /db_xref="MGI:MGI:2685903"
                     /translation="MRPPALLALFSCSAAFALMSEEIKEKVTPSQDLRQSSLPGRHDI
                     DLKEIVFVIQSQSNSFHAKRAEQLKKNILKQAANLTQDLPRVLLLHQLAKQEGAWTIL
                     PLLPHFSVTYSKNSAWIFFCEEETRLQIPRLLDTLRRYDPSKEWFLGKALYDEESTII
                     HHYAFSENPTVFKYPDFAAGWALSIPLVNKLAKRLKSEALKSDFTIDLKHEIALYIWD
                     KGGGPALTPVPEFCTEDVDPRCVTTFHSFLPLCGVPVKKEEIFVAVKTCKKFHADRIP
                     IVKKTWAAQASLIEYYSDYAETAIPTVDLGIPNTDRGHCGKTFAILEKFLNHSHNKIS
                     WLVIVDDDTLISISRLRHLLSCYDSSDPVFLGERYGYGLGTGGYSYVTGGGGMVFSRE
                     AIRRLLVSSCRCYSNDAPDDMVLGMCFSGLGVPVTHSPLFHQARPVDYPKDYLAHQIP
                     VSFHKHWHIDPVKVYLTWLAPSEEDQATQETQKDPREEL"
     exon            151..200
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            201..240
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            241..350
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            351..427
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            428..539
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            540..676
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            677..740
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            741..860
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            861..930
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1044
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1144
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1264
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1409
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..4570
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1835..1840
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /note="hexamer: AATAAA"
     polyA_site      1855
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
     regulatory      4549..4554
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /note="hexamer: AATAAA"
     polyA_site      4570
                     /gene="B3glct"
                     /gene_synonym="B3galtl; beta3Glc-T; Gm1057"
                     /note="major polyA site"
ORIGIN      
        1 gagccggcgc ggcgccggga aggaggaggg gagcgcgaga agggtcaggc gcgcagggcg
       61 tccgcgcagc tcggctccgg gaagggcgcg gagcctctgc ctccgggatg cggcctcccg
      121 cgctgctggc gctgttcagc tgctccgcgg cttttgcttt gatgtctgaa gagataaaag
      181 aaaaggtcac gccttctcag gatttgaggc agagtagtct accggggaga catgacatag
      241 acttaaaaga aatcgtgttt gtcatccaga gtcaaagtaa ttcctttcat gccaagagag
      301 cagaacagtt gaaaaaaaac atcttaaagc aggctgcaaa tctgacacag gatctcccta
      361 gagtcctcct tctccatcag ttggcaaagc aggaaggtgc ttggaccatc cttcccttgt
      421 taccgcactt ttctgtaaca tacagcaaga attcagcgtg gattttcttc tgtgaagaag
      481 agacgagact acaaatcccc agactcctag acacactcag acgatatgac ccatctaagg
      541 aatggttttt aggaaaagca ctgtatgacg aagagtcgac gatcatccac cattacgcct
      601 tctctgagaa cccaacagtc tttaaatatc ccgacttcgc tgctggctgg gcccttagca
      661 tcccgctggt gaacaagctt gccaaaagat taaagagtga ggcgctgaag tctgacttca
      721 caattgattt aaagcacgag attgccctct acatctggga caaaggtgga ggaccggccc
      781 tcacccctgt gcctgagttt tgtacagaag atgtggaccc ccgctgcgtc accacgttcc
      841 attctttcct accgctctgt ggagtaccgg taaagaagga ggagattttt gttgcagtga
      901 aaacgtgcaa gaaatttcat gctgacagaa tacccatcgt aaagaagaca tgggcggcac
      961 aggctagcct tatcgaatac tacagtgact atgcagaaac cgccatcccc acggtggatt
     1021 tgggaattcc caatacagac aggggtcatt gtggaaaaac ctttgccatt ttggaaaaat
     1081 ttttgaatca cagtcacaac aaaatctcat ggttagtcat tgtggacgat gacacattaa
     1141 tcagcatctc caggctccga cacctgctta gctgctatga ctccagcgac cctgtgtttc
     1201 tgggagagcg ctatggctac ggcctaggca caggcggcta cagctatgtc acaggaggag
     1261 gcgggatggt ctttagcagg gaagccatcc ggagacttct cgtgagcagc tgcagatgct
     1321 acagcaatga cgctccggac gacatggttc tgggcatgtg cttcagtggg ctgggggtcc
     1381 ccgtgacaca cagccccctc ttccatcagg ctcggccagt agattacccc aaggactacc
     1441 tcgcccatca gattccagta tcatttcaca aacactggca cattgacccg gtaaaggtgt
     1501 acctcacgtg gctggcaccc agtgaggaag accaagccac gcaggagaca cagaaggacc
     1561 caagagaaga attatagaac agagtctggg acacacggat acaagagatt caccctgtct
     1621 gttgagctgt ggtccagctg ctcgtgtatg ctgtgtgaaa tggacacttc ccagtccttg
     1681 tggaaatagg atattttctg tatcggtaga ctcataaggg aaactgggaa aaatcgcttg
     1741 ttttcataga tgtcgtaatt gtaacacggc aattattgtg cctattttag cccaccgtgg
     1801 gctgtaaatc aagagctttt tgactgtcag aggaaataaa tggtacctaa agtccttttc
     1861 tgttttctcc attgtaacag ggacgccaat acaagaatat gactggctat catcccacat
     1921 aaacattgaa tctttaaaca tggatattgc caaaggaatg agcctttaaa ccaatggttc
     1981 tcaacctgtg ggtcgagacc ccctaggagt ggcagttggt cctatgtcag gaatcctaca
     2041 tattaggtac ttacattgca attcgtaatt gtagaaaaat tacagttatg aagtagcaag
     2101 gaaatagttt tatggttgta ggtcacctca gcatgaagga actactgtat taaaggacca
     2161 ctgcctaagg aaggttgaga accatggctt taaatggctc tattgttgat ggaaattggt
     2221 tcccaggcac tcagtaggaa tgaaatactg aggctgattg gtaaaaccta aaactcaata
     2281 tgaatacccc caactataca atcacggttg ctttttcccc tattctgctg gttgcacccc
     2341 caaaacagac tcaaggccat ctggtgaagg ctctgctccc tgccagcctc cccaaagccc
     2401 tccgccccca atagagtgga cagagtgtca ccggaaatta ggatgctagg ttgacaggtc
     2461 tcttctgtgt cagcagtggg cgtggggggg tctggtaatg tgagctgcat ctggagctgg
     2521 cctgttactc ctgctgttcc ctgggtgtta catagtaact cgcttctggc tctggctcag
     2581 gctagactgc ctccgtcctg gcagcatcag catggcccct tgtgagagga cagcatggag
     2641 atcagcaatt tctctgggtt ttaaaagaat gaaatctgaa caacatgctt taaagcaacc
     2701 ctcgttttcc agtgacacct tttattatgt cctaatttaa caattcagcg ttaatatata
     2761 taaaaaaaga gtactgtgtg ttaatgttta aagtaagatg atgattttgt tatataaatg
     2821 tctttgcaca tgatttgctc tcaagttcct aaggttgtat gttagaatcc tttccttttt
     2881 ttcaatctgt acttcattaa ccttacttat aaggctccta agcccttata gcctttaaag
     2941 ccactccatc agcaatacct atgtgattac tttttaacaa tgtatattcc tatgtagctt
     3001 acttctgact catgtccaaa ttcatgcagt agtgtttatc atccttttcc caaattaata
     3061 agcaccagga ttggtgtgtg gcccactact tctggtttct ggtggaacac agactatgaa
     3121 aactgattct gtggttcagc tagaaactga actgaggaca gtcaagtgca tgaaagggac
     3181 cataatggct tctgggtgtc ctgcagatgg ctgtagcatc attgctgaca cggcaatgat
     3241 gaatgccacc attgtgacca ttgctatcat ggtacttacc ttcttgacct tgcagggata
     3301 ccccgggggg acctggttgc tacaccagtg ccgctcaggg aattcctcag tccacccctc
     3361 ccatctctgc tcctgaaatg ttaccaacca gagaacagag gaagtttcat ttggtaggac
     3421 aaaagctagc gcatctgagg aatgaagaat gttcaatctt ttttctctgt tatgacctgt
     3481 gccctctttc cccctcctcc ttcccctccc cctcccccct ccctccagga tcctgctggt
     3541 atttctagat atgtggttaa ctatgtttcc cttttggagc tacagaagtc tcatcatgaa
     3601 tacaatgtct tcagcttact tctgaccttg cattttgtct attggttgag gctagatgtg
     3661 gggtgggtag gagcatgctg gacaggcgct gagaaattgg gaacactggc agattactaa
     3721 gacagtcaat gaaaactaaa catcttagga aaaagcaaag aaaaaaaaac agttttaaaa
     3781 gaaccacgtg ttatgtttta tacatagcaa atggcaggct ctatttgaac accaactgag
     3841 aattgatatc ttaaaaaaaa agaaaaaaca aaacaaaaca aaaaacctta attttgagcc
     3901 tggattcttc agggcacatc cctccctctt tggtattctt tttcccttta agtaaggatt
     3961 tctttgccag tagatatttt tatcctaagg atttagcacc aaggatttat gtatagaatt
     4021 acctgatagg tcatttttac ttttaagcca tttctcatga cccagcacat cgcctgcctg
     4081 tagaattttt acatcttaca gctaaatcag aagtcgagac acaaaaataa cctttgttga
     4141 atgcttggga ccgtaatcgc tcagcagcca gagttacaaa caatgctacg cagggccgtg
     4201 ttagatgcca gtgttcactg cgtgtcctga ttgatcgacg acatttagta cgttgcatct
     4261 atcatggtta ttgatgtgta tagagaacct cagagattca ccacacagta gacatttgta
     4321 agaatatttg tggtgcctcc cgttccttcg tagatgtgtc atttttgtgg aagaaaaaaa
     4381 aaagcaatca atgtaccatt aagtacacta tagcagactt acaaaggggc tttgttcctg
     4441 ctgtctttga atgacaggcc tgaaaaggtg ctatttaaat tgtttatgtc ctcaagccct
     4501 ctaaacaagt cacctgcaaa atgtgttctc ctcctttcta tagaataaaa taaaggattt
     4561 gtttctggta
//
