LOCUS       NM_177746               1632 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus acyl-CoA wax alcohol acyltransferase 2 (Awat2),
            transcript variant 1, mRNA.
ACCESSION   NM_177746
VERSION     NM_177746.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Hisey,E.A., Wong,S., Park,S., Gamarra,K.A., Adelman,S.A.,
            Knickelbein,K.E., Quan,M., Ferneding,M.H., McCorkell,M., Daley,N.,
            Ureno,V., Le,S., Ardon,M., Williams,L., Puentes,B., Bowman,M.,
            Motta,M.J., Pham,H.Q.H., Wilkerson,A., Yuksel,S., Jester,J.V.,
            Thomasy,S.M., Morgan,J.T., Butovich,I.A. and Leonard,B.C.
  TITLE     Meibomian gland lipid alterations and ocular surface sequela in
            Awat2 knockout murine model of meibomian gland dysfunction and
            evaporative dry eye disease
  JOURNAL   Ocul Surf 34, 489-503 (2024)
   PUBMED   39414024
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Puentes,B., Hisey,E.A., Ferneding,M., Ureno,V.N., Do,M.A.H.,
            Karpinen,P.M., Luo,C.C., Thomasy,S.M. and Leonard,B.C.
  TITLE     Development and validation of a method to generate phenol red
            thread tests
  JOURNAL   Ocul Surf 34, 262-266 (2024)
   PUBMED   39127389
REFERENCE   3  (bases 1 to 1632)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1632)
  AUTHORS   Widjaja-Adhi,M.A.K., Kolesnikov,A.V., Vasudevan,S., Park,P.S.,
            Kefalov,V.J. and Golczak,M.
  TITLE     Acyl-CoA:wax alcohol acyltransferase 2 modulates the cone visual
            cycle in mouse retina
  JOURNAL   FASEB J 36 (7), e22390 (2022)
   PUBMED   35665537
  REMARK    GeneRIF: Acyl-CoA:wax alcohol acyltransferase 2 modulates the cone
            visual cycle in mouse retina.
REFERENCE   5  (bases 1 to 1632)
  AUTHORS   Sawai,M., Watanabe,K., Tanaka,K., Kinoshita,W., Otsuka,K.,
            Miyamoto,M., Sassa,T. and Kihara,A.
  TITLE     Diverse meibum lipids produced by Awat1 and Awat2 are important for
            stabilizing tear film and protecting the ocular surface
  JOURNAL   iScience 24 (5), 102478 (2021)
   PUBMED   34113821
  REMARK    Erratum:[iScience. 2021 Sep 25;24(10):103160. doi:
            10.1016/j.isci.2021.103160.. PMID: 34622178]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1632)
  AUTHORS   Arne,J.M., Widjaja-Adhi,M.A.K., Hughes,T., Huynh,K.W.,
            Silvaroli,J.A., Chelstowska,S., Moiseenkova-Bell,V.Y. and
            Golczak,M.
  TITLE     Allosteric modulation of the substrate specificity of acyl-CoA wax
            alcohol acyltransferase 2
  JOURNAL   J Lipid Res 58 (4), 719-730 (2017)
   PUBMED   28096191
  REMARK    GeneRIF: hese data provide the experimental basis for understanding
            regulation of AWAT2 substrate specificity.
REFERENCE   7  (bases 1 to 1632)
  AUTHORS   Alvarez-Dominguez,J.R., Zhang,X. and Hu,W.
  TITLE     Widespread and dynamic translational control of red blood cell
            development
  JOURNAL   Blood 129 (5), 619-629 (2017)
   PUBMED   27899360
REFERENCE   8  (bases 1 to 1632)
  AUTHORS   Kawelke,S. and Feussner,I.
  TITLE     Two Predicted Transmembrane Domains Exclude Very Long Chain Fatty
            acyl-CoAs from the Active Site of Mouse Wax Synthase
  JOURNAL   PLoS One 10 (12), e0145797 (2015)
   PUBMED   26714272
  REMARK    GeneRIF: Transmembrane Domains Exclude Very Long Chain Fatty
            acyl-CoAs from the Active Site of Mouse Wax Synthase
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1632)
  AUTHORS   Alvarez-Dominguez,J.R., Hu,W., Yuan,B., Shi,J., Park,S.S.,
            Gromatzky,A.A., van Oudenaarden,A. and Lodish,H.F.
  TITLE     Global discovery of erythroid long noncoding RNAs reveals novel
            regulators of red cell maturation
  JOURNAL   Blood 123 (4), 570-581 (2014)
   PUBMED   24200680
REFERENCE   10 (bases 1 to 1632)
  AUTHORS   Cheng,J.B. and Russell,D.W.
  TITLE     Mammalian wax biosynthesis. II. Expression cloning of wax synthase
            cDNAs encoding a member of the acyltransferase enzyme family
  JOURNAL   J Biol Chem 279 (36), 37798-37807 (2004)
   PUBMED   15220349
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY611032.1 and AY611031.1.
            
            On Mar 12, 2014 this sequence version replaced NM_177746.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY611031.1, AY611032.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849381,
                                           SAMN01164135 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1345              AY611032.1         1-1345
            1346-1632           AY611031.1         1321-1607
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 43.71 cM"
     gene            1..1632
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /note="acyl-CoA wax alcohol acyltransferase 2"
                     /db_xref="GeneID:245532"
                     /db_xref="MGI:MGI:3045345"
     exon            1..137
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..1054
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /EC_number="2.3.1.20"
                     /EC_number="2.3.1.75"
                     /EC_number="2.3.1.76"
                     /note="isoform 1 is encoded by transcript variant 1; wax
                     synthase 2; long-chain-alcohol O-fatty-acyltransferase 2;
                     diacylglycerol O-acyltransferase 2-like protein 4;
                     acyl-CoA retinol O-fatty-acyltransferase;
                     11-cis-RE-synthase; 11-cis-specific retinyl-ester
                     synthase"
                     /codon_start=1
                     /product="acyl-CoA wax alcohol acyltransferase 2 isoform
                     1"
                     /protein_id="NP_808414.2"
                     /db_xref="CCDS:CCDS30300.1"
                     /db_xref="GeneID:245532"
                     /db_xref="MGI:MGI:3045345"
                     /translation="MFWPTKKDLKTAMEVFALFQWALSALVIVTTVIIVNLYLVVFTS
                     YWPVTVLMLTWLAFDWKTPERGGRRFTCVRKWRLWKHYSDYFPLKMVKTKDISPDRNY
                     ILVCHPHGLMAHSCFGHFATDTTGFSKTFPGITPYMLTLGAFFWVPFLRDYVMSTGSC
                     SVSRSSMDFLLTQKGTGNMLVVVVGGLAECRYSTPGSTTLFLKKRQGFVRTALKHGVS
                     LIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQKMVHIYPCAFYGRGLTKNSWGLLPYS
                     QPVTTVVGEPLPLPKIENPSEEIVAKYHTLYIDALRKLFDQHKTKFGISETQELVIV"
     exon            138..248
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /inference="alignment:Splign:2.1.0"
     exon            249..319
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /inference="alignment:Splign:2.1.0"
     exon            320..524
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /inference="alignment:Splign:2.1.0"
     exon            525..699
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /inference="alignment:Splign:2.1.0"
     exon            700..899
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1619
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1324..1329
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /note="hexamer: AATAAA"
     polyA_site      1345
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /note="major polyA site"
     regulatory      1585..1590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
                     /note="hexamer: ATTAAA"
     polyA_site      1619
                     /gene="Awat2"
                     /gene_synonym="9430062J17Rik; ARAT; Dgat2l4; WS"
ORIGIN      
        1 gggctccaga gccaaacagc gtctacctgg aactcctgag attctgggca ccatgttctg
       61 gcccaccaag aaggacctca agaccgccat ggaggtcttt gctcttttcc agtgggccct
      121 cagtgccttg gttatagtca ccactgtgat cattgtcaac ctctacttgg tggtgttcac
      181 atcttactgg cctgtcaccg tgttgatgct cacctggctg gcttttgact ggaagacccc
      241 tgagcgaggt ggccgcaggt tcacctgtgt gaggaagtgg cgtctgtgga aacactatag
      301 cgactacttc ccactcaaga tggtgaagac gaaagatatt tcacctgacc gcaactacat
      361 ccttgtctgc catcctcatg ggctcatggc ccattcatgt tttggtcact ttgccactga
      421 cacaacaggc ttttccaaga cctttcctgg tatcactcct tatatgctca cactaggagc
      481 ctttttctgg gtacctttcc tcagagatta tgtaatgtct acagggtcgt gctctgtgag
      541 tagatcctcc atggactttt tgcttaccca gaagggcaca ggcaacatgc ttgtggtggt
      601 ggttggtggc ctggctgagt gcagatacag cacgccaggc tctaccaccc tgttcttgaa
      661 gaagcggcaa ggctttgtgc gcacagccct taaacatggg gtgtctctaa tcccagctta
      721 tgcctttgga gagacagacc tctatgacca gcacattttc actcctgggg gctttgtcaa
      781 tcgcttccag aagtggttcc agaagatggt acacatctac ccctgtgctt tctatgggcg
      841 tggcctcacc aagaactcct ggggccttct gccctattct cagccagtaa ccactgttgt
      901 tggagaacct ctaccattgc ccaagattga gaatccgagc gaggagattg tggccaaata
      961 ccacacactc tatattgatg ccctacgaaa attgtttgat cagcataaga ccaagtttgg
     1021 catctcagag acccaggagc tggtgatagt ttgaaagaca ttcccttgac tggatggtgt
     1081 ggaactgatg acaacagggc aaggttacct gtggagcttc acttctgact tgactggtcc
     1141 tcactggagg gaggtggggg tgtgcatcca tgtggttgtt ttctgtgccc atcctttccc
     1201 acctccctct ctttctctta gtttttgtct ttttcccctg agacagagaa gactctaggc
     1261 aaaagtccac tgaagtcatt ggactttcat gaaagcatta gcagcaagga aacctgtagg
     1321 tggaataaac agcccatctc tggtactgtg tgctccagct ggtctgtttg gagggctcta
     1381 agaccttgtg tgtctgccat tgtcctggct tcaagactct tacaggactg ggtatctcaa
     1441 gagccagggg acattgggtt cagtccgtag catgctcaag acctatgatg tttaagggca
     1501 atccgtcacc ttttatcttt tttctatagc tataaaatgg gactaacaat ccttcccttg
     1561 cttacctcaa aggtttctgt caaaattaaa tgctactgca tgtgcctgaa catgataaaa
     1621 aaaaaaaaaa aa
//
