LOCUS       NM_001081136            1395 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus acyl-CoA wax alcohol acyltransferase 1 (Awat1), mRNA.
ACCESSION   NM_001081136 XM_141972
VERSION     NM_001081136.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Veniaminova,N.A., Jia,Y.Y., Hartigan,A.M., Huyge,T.J., Tsai,S.Y.,
            Grachtchouk,M., Nakagawa,S., Dlugosz,A.A., Atwood,S.X. and
            Wong,S.Y.
  TITLE     Distinct mechanisms for sebaceous gland self-renewal and
            regeneration provide durability in response to injury
  JOURNAL   Cell Rep 42 (9), 113121 (2023)
   PUBMED   37715952
REFERENCE   3  (bases 1 to 1395)
  AUTHORS   Sawai,M., Watanabe,K., Tanaka,K., Kinoshita,W., Otsuka,K.,
            Miyamoto,M., Sassa,T. and Kihara,A.
  TITLE     Diverse meibum lipids produced by Awat1 and Awat2 are important for
            stabilizing tear film and protecting the ocular surface
  JOURNAL   iScience 24 (5), 102478 (2021)
   PUBMED   34113821
  REMARK    Erratum:[iScience. 2021 Sep 25;24(10):103160. doi:
            10.1016/j.isci.2021.103160.. PMID: 34622178]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1395)
  AUTHORS   Miklaszewska,M., Kawinski,A. and Banas,A.
  TITLE     Detailed characterization of the substrate specificity of mouse wax
            synthase
  JOURNAL   Acta Biochim Pol 60 (2), 209-215 (2013)
   PUBMED   23730681
  REMARK    GeneRIF: The highest activity was observed for 14:0-CoA, 12:0-CoA,
            and 16:0-CoA in combination with medium chain alcohols (up to 5.2,
            3.4, and 3.3 nmol wax esters/min/mg microsomal protein,
            respectively
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL671299.18.
            
            On Feb 6, 2007 this sequence version replaced XM_141972.6.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK137763.1, BC147683.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-166               AL671299.18        177663-177828
            167-274             AL671299.18        178885-178992
            275-345             AL671299.18        179246-179316
            346-550             AL671299.18        180115-180319
            551-722             AL671299.18        180938-181109
            723-922             AL671299.18        182762-182961
            923-1395            AL671299.18        183150-183622
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 43.72 cM"
     gene            1..1395
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /note="acyl-CoA wax alcohol acyltransferase 1"
                     /db_xref="GeneID:245533"
                     /db_xref="MGI:MGI:3588200"
     exon            1..166
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1077
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /EC_number="2.3.1.75"
                     /note="wax synthase 1; long-chain-alcohol
                     O-fatty-acyltransferase 1; diacylglycerol
                     O-acyltransferase 2-like protein 3"
                     /codon_start=1
                     /product="acyl-CoA wax alcohol acyltransferase 1"
                     /protein_id="NP_001074605.1"
                     /db_xref="CCDS:CCDS41074.1"
                     /db_xref="GeneID:245533"
                     /db_xref="MGI:MGI:3588200"
                     /translation="MSCSMKTEHLQSLSLLQWPLSYVAMFWIVQPLLICLLFTPLWPL
                     PTVYFVWLLLDWKTPDKGGRRSDWVRNWNVWNHIRDYFPITILKTKDLSPSENYIMGV
                     HPHGLLTFGAFCNFCTEATGFSKTFPGITPHLATLSWFFKIPIIRDYIMAKGLCSVSQ
                     ASIDYLLSHGTGNLVGIVVGGVGEALQSVPNTTTLLLKKRKGFVRTALQHGAHLVPTF
                     TFGETEVYDQVLFHEDSRMFKFQSLFRRIFGFYCCVFYGQGFHQDCKGLLPYHKPIIT
                     VVGEALPLPQVKNPSPEIVDKYHALYMDALYKLFEQHKVQYGCSNTQKLIFL"
     exon            167..274
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            275..345
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            346..550
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            551..722
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            723..922
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1395
                     /gene="Awat1"
                     /gene_synonym="Dgat2l3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 attcactgct aagtgagcat ccagagactg gtggcatcgt tcattggaac aagctctagg
       61 aactttacct ccctcagggt cctgggcatc atgtcttgct ctatgaagac tgaacactta
      121 cagagtctga gccttctgca gtggcccttg agctacgttg ccatgttttg gattgtgcag
      181 ccattgttaa tttgcctatt gttcacaccc ttgtggccgc taccaacagt ttactttgtc
      241 tggttacttc tcgactggaa gactccagat aaaggtggca ggcgttcaga ctgggtacgg
      301 aactggaatg tctggaacca catcagggac tatttcccca ttacaatcct gaagactaag
      361 gacctgtcac cttcagagaa ctacatcatg ggggtccacc cccatggtct cctgaccttc
      421 ggtgccttct gcaacttctg cactgaggcc acaggcttct cgaagacctt cccaggcatc
      481 actcctcact tggccacact gtcctggttc ttcaagatcc ccattattag ggactacatc
      541 atggccaaag gattgtgttc tgtgagccag gcatccatcg actacctgct gagccatggc
      601 actggaaacc tcgtgggcat tgtcgtggga ggagtgggag aggccctaca gagtgtgcct
      661 aacaccacca ccctcctcct caagaaacgc aaagggtttg tgcgcacagc cctccaacat
      721 ggggctcatc tggtccctac cttcacgttc ggagaaacag aggtatatga ccaggtactg
      781 tttcatgagg atagccggat gttcaagttc caaagcctct ttcgccggat ctttggtttc
      841 tattgctgtg tcttctatgg acaaggtttc catcaagact gcaagggact cctaccatac
      901 cacaaaccca tcatcactgt agttggggaa gctttgccac tgccccaggt taaaaaccca
      961 agcccagaga tagtggacaa ataccatgca ctctacatgg acgccctgta caagctgttt
     1021 gagcagcaca aggtccagta tggctgctcc aacacccaga agctgatatt cttgtgatca
     1081 aagtgtctgt gcactctaga gaagggcggc acctgctttg aagaagaaac tgtgttgcct
     1141 agaaccggag ttctggaaaa ggggaaaagg aaggaagatg aaggaggctg tgtgtgcagt
     1201 catggaaggg gcattaagag ctccttcctg accataatac tgcccctgcc tgcacccctg
     1261 caggagttgt ccctgaggat ccccacccag gcattcttca gaggggacac ccttagactc
     1321 ctcttgtcac acgtactttc tgaacctgaa atttttaaat ccagtttttt aaaatgttaa
     1381 taaaaagatg aaatt
//
