LOCUS       NM_007517               1435 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus ancient ubiquitous protein 1 (Aup1), transcript
            variant 1, mRNA.
ACCESSION   NM_007517 XM_620335
VERSION     NM_007517.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Lohmann,D., Spandl,J., Stevanovic,A., Schoene,M.,
            Philippou-Massier,J. and Thiele,C.
  TITLE     Monoubiquitination of ancient ubiquitous protein 1 promotes lipid
            droplet clustering
  JOURNAL   PLoS One 8 (9), e72453 (2013)
   PUBMED   24039768
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Jo,Y., Hartman,I.Z. and DeBose-Boyd,R.A.
  TITLE     Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination
            of 3-hydroxy-3-methylglutaryl CoA reductase in lipid
            droplet-associated endoplasmic reticulum membranes
  JOURNAL   Mol Biol Cell 24 (3), 169-183 (2013)
   PUBMED   23223569
REFERENCE   3  (bases 1 to 1435)
  AUTHORS   Klemm,E.J., Spooner,E. and Ploegh,H.L.
  TITLE     Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet
            accumulation and endoplasmic reticulum (ER) protein quality control
  JOURNAL   J Biol Chem 286 (43), 37602-37614 (2011)
   PUBMED   21857022
REFERENCE   4  (bases 1 to 1435)
  AUTHORS   Jang,W., Weber,J.S., Bashir,R., Bushby,K. and Meisler,M.H.
  TITLE     Aup1, a novel gene on mouse chromosome 6 and human chromosome 2p13
  JOURNAL   Genomics 36 (2), 366-368 (1996)
   PUBMED   8812468
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104324.21.
            
            On Feb 19, 2021 this sequence version replaced NM_007517.4.
            
            Summary: The protein encoded by this gene contains several
            conserved domains including a hydrophobic domain, an
            acetyltransferase domain, a ubiquitin binding domain, and a domain
            required for recruitment of ubiquitin-conjugating enzyme E2 G2
            (Ube2g2). In humans, this protein localizes to the endoplasmic
            reticulum and to lipid droplets. This protein is thought to be
            involved both in the degradation of misfolded proteins from the
            endoplasmic reticulum and in the storage of neutral lipids. Reduced
            expression of the human ortholog of this gene strongly reduces
            lipid droplet clustering in the cell, and causes stabilization of
            misfolded proteins. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Aug 2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016485.1, U41736.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               AC104324.21        91270-91373
            105-242             AC104324.21        91452-91589
            243-393             AC104324.21        91670-91820
            394-578             AC104324.21        92047-92231
            579-651             AC104324.21        92439-92511
            652-725             AC104324.21        92608-92681
            726-792             AC104324.21        92909-92975
            793-895             AC104324.21        93083-93185
            896-1015            AC104324.21        93300-93419
            1016-1131           AC104324.21        93519-93634
            1132-1250           AC104324.21        93817-93935
            1251-1435           AC104324.21        94039-94223
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 35.94 cM"
     gene            1..1435
                     /gene="Aup1"
                     /note="ancient ubiquitous protein 1"
                     /db_xref="GeneID:11993"
                     /db_xref="MGI:MGI:107789"
     exon            1..104
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1287
                     /gene="Aup1"
                     /note="isoform 1 is encoded by transcript variant 1; lipid
                     droplet-regulating VLDL assembly factor AUP1"
                     /codon_start=1
                     /product="lipid droplet-regulating VLDL assembly factor
                     AUP1 isoform 1"
                     /protein_id="NP_031543.3"
                     /db_xref="CCDS:CCDS39526.1"
                     /db_xref="GeneID:11993"
                     /db_xref="MGI:MGI:107789"
                     /translation="MEPPPAPGPERLFDSHRLPSDGFLLLALLLYAPVGLCLLVLRLF
                     LGLHVFLVSCALPDSVLRRFVVRTMCAVLGLVARQEDSGLRDHRVRVLISNHVTPFDH
                     NIVNLLTTCSTPLLNSPPSFVCWSRGFMEMDRRVELVESLKKFCASTRLPPTPLLLFP
                     EEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSVTVSDASWVSELLWSLFVPF
                     TVYQVRWLHPIRRQLGEESEEFALRVQQLVAKELGQIGTRLTPADKAEHMKRQRHPRL
                     RPQSVQSSFPSPPSPSSDVQLTTLAHRVKEVLPHVPLNVIQRDLARTGCVDLTITNLL
                     EGAVAFMPEDVTEGSQSPPAPSAPKFPSSGLATPQPTALTFAKSSWARQESLQERKQA
                     LYEYARRRFRERQAQEAE"
     exon            105..242
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            243..393
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            394..578
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            579..651
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            652..725
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            726..792
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            793..895
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1015
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1131
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1250
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1435
                     /gene="Aup1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1412..1417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aup1"
                     /note="hexamer: ATTAAA"
     polyA_site      1435
                     /gene="Aup1"
                     /note="major polyA site"
ORIGIN      
        1 gcggccctgc gaagatggcg gcgacagcgg ccccgctctc ctgaagcggc tgcgatggaa
       61 cctcccccag ctccggggcc ggagcggctc tttgactcgc accggctccc gagtgacggc
      121 ttcctgctgc tcgcgctact gctctacgcg ccggtcggcc tctgcctcct ggtcctgcga
      181 ctcttcctcg ggctccacgt cttcctggtc agctgcgctc tgcccgacag cgtcctccgc
      241 aggttcgtgg tacgaaccat gtgtgcggtc ctggggctcg tggcccggca ggaggactcc
      301 ggactccgcg atcaccgcgt cagggtcctt atttccaacc acgtaacacc ttttgaccac
      361 aacatagtca acctcctcac cacctgtagc acccctctac tcaatagtcc ccccagcttt
      421 gtgtgttggt ctcggggctt catggagatg gataggcggg tggagttggt ggagtcactc
      481 aagaaattct gtgcttccac gaggcttccg cccacacctt tgctgctctt ccccgaggaa
      541 gaggccacca atggccgaga agggctgctg cgtttcagtt cgtggccatt ttctattcag
      601 gacgtggtac aacctcttac cctgcaagtt cagagacccc tggtctctgt gacggtgtca
      661 gatgcctcct gggtctcaga actgctgtgg tccctttttg ttcctttcac ggtgtatcaa
      721 gtaaggtggc ttcatcccat tcgtcgacag ctaggggaag agagtgagga gtttgcgctc
      781 cgtgtacaac agctggtggc caaagaattg ggccagatag ggacacggct cactccagca
      841 gacaaagcag aacacatgaa gcgacaaaga caccccagat tacgccccca gtcagtgcag
      901 tcttcttttc cttctcctcc cagcccttct tctgatgtgc agctgaccac tctcgctcac
      961 agagtcaagg aggttctgcc ccatgtgcca ttgaatgtca tccagagaga cctggccagg
     1021 actgggtgtg tagacttgac catcacaaac ctgctcgagg gggctgtggc tttcatgcct
     1081 gaagatgtca ctgagggatc tcagtccccg cctgcaccct ctgccccaaa gttccccagc
     1141 tcgggcctgg cgacccctca gcccacagcc ctaacgtttg ccaagtcttc ctgggcccgt
     1201 caggagagcc tgcaggagcg caagcaggca ctgtatgaat atgcaagaag gagattcaga
     1261 gagagacagg cccaggaggc tgaatgagcc caaaggaaca ggatggtacc cagagccgca
     1321 ggacggagac tgggggcagc cctcacccag ctaacaacag gccggatgag tgggtggtaa
     1381 aaagggaggg atggggctcc cccaatctca cattaaattc agggttttca ctcaa
//
