LOCUS       NM_001001488            6440 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), mRNA.
ACCESSION   NM_001001488
VERSION     NM_001001488.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Tamura,R., Sabu,Y., Mizuno,T., Mizuno,S., Nakano,S., Suzuki,M.,
            Abukawa,D., Kaji,S., Azuma,Y., Inui,A., Okamoto,T., Shimizu,S.,
            Fukuda,A., Sakamoto,S., Kasahara,M., Takahashi,S., Kusuhara,H.,
            Zen,Y., Ando,T. and Hayashi,H.
  TITLE     Intestinal Atp8b1 dysfunction causes hepatic choline deficiency and
            steatohepatitis
  JOURNAL   Nat Commun 14 (1), 6763 (2023)
   PUBMED   37990006
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6440)
  AUTHORS   Yang,W.J., Cao,R.C., Xiao,W., Zhang,X.L., Xu,H., Wang,M.,
            Zhou,Z.T., Chen,H.J., Xu,J., Chen,X.M., Zeng,J.L., Li,S.J., Luo,M.,
            Han,Y.J., Yang,X.B., Feng,G.D., Lu,Y.H., Ni,Y.Y., Wu,C.G.,
            Bai,J.J., Yuan,Z.Q., Jin,J. and Zhang,G.W.
  TITLE     Acinar ATP8b1/LPC pathway promotes macrophage efferocytosis and
            clearance of inflammation during chronic pancreatitis development
  JOURNAL   Cell Death Dis 13 (10), 893 (2022)
   PUBMED   36273194
  REMARK    GeneRIF: Acinar ATP8b1/LPC pathway promotes macrophage
            efferocytosis and clearance of inflammation during chronic
            pancreatitis development.
            Erratum:[Cell Death Dis. 2022 Nov 7;13(11):930. doi:
            10.1038/s41419-022-05374-8.. PMID: 36344486]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6440)
  AUTHORS   Westermann-Clark,E., Soundararajan,R., Fukumoto,J., Patil,S.S.,
            Stearns,T.M., Saji,S., Czachor,A., Hernandez-Cuervo,H.,
            Breitzig,M., Krishnamurthy,S., Lockey,R.F. and Kolliputi,N.
  TITLE     Matrix Metalloproteinase 7 Expression and Apical Epithelial Defects
            in Atp8b1 Mutant Mouse Model of Pulmonary Fibrosis
  JOURNAL   Biomolecules 12 (2), 283 (2022)
   PUBMED   35204783
  REMARK    GeneRIF: Matrix Metalloproteinase 7 Expression and Apical
            Epithelial Defects in Atp8b1 Mutant Mouse Model of Pulmonary
            Fibrosis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6440)
  AUTHORS   Langedijk,J., Bolier,R., Tolenaars,D., Ten Bloemendaal,L.,
            Duijst,S., de Waart,D., Beuers,U., Bosma,P. and Elferink,R.O.
  TITLE     Reduced spontaneous itch in mouse models of cholestasis
  JOURNAL   Sci Rep 11 (1), 6127 (2021)
   PUBMED   33731871
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6440)
  AUTHORS   van Mil,S.W., van Oort,M.M., van den Berg,I.E., Berger,R.,
            Houwen,R.H. and Klomp,L.W.
  TITLE     Fic1 is expressed at apical membranes of different epithelial cells
            in the digestive tract and is induced in the small intestine during
            postnatal development of mice
  JOURNAL   Pediatr Res 56 (6), 981-987 (2004)
   PUBMED   15496606
  REMARK    GeneRIF: Fic1 is expressed in a tissue-specific and developmentally
            regulated fashion at the apical membranes of epithelial cells
REFERENCE   7  (bases 1 to 6440)
  AUTHORS   Pawlikowska,L., Groen,A., Eppens,E.F., Kunne,C., Ottenhoff,R.,
            Looije,N., Knisely,A.S., Killeen,N.P., Bull,L.N., Elferink,R.P. and
            Freimer,N.B.
  TITLE     A mouse genetic model for familial cholestasis caused by ATP8B1
            mutations reveals perturbed bile salt homeostasis but no impairment
            in bile secretion
  JOURNAL   Hum Mol Genet 13 (8), 881-892 (2004)
   PUBMED   14976163
  REMARK    GeneRIF: mutation disrupts bile salt homeostasis without impairment
            of canalicular bile secretion
REFERENCE   8  (bases 1 to 6440)
  AUTHORS   Ujhazy,P., Ortiz,D., Misra,S., Li,S., Moseley,J., Jones,H. and
            Arias,I.M.
  TITLE     Familial intrahepatic cholestasis 1: studies of localization and
            function
  JOURNAL   Hepatology 34 (4 Pt 1), 768-775 (2001)
   PUBMED   11584374
  REMARK    Erratum:[Hepatology 2002 Jan;35(1):246]
REFERENCE   9  (bases 1 to 6440)
  AUTHORS   Lammert,F., Carey,M.C. and Paigen,B.
  TITLE     Chromosomal organization of candidate genes involved in cholesterol
            gallstone formation: a murine gallstone map
  JOURNAL   Gastroenterology 120 (1), 221-238 (2001)
   PUBMED   11208732
  REMARK    Review article
REFERENCE   10 (bases 1 to 6440)
  AUTHORS   Halleck,M.S., Lawler,J.F. Jr., Blackshaw,S., Gao,L., Nagarajan,P.,
            Hacker,C., Pyle,S., Newman,J.T., Nakanishi,Y., Ando,H.,
            Weinstock,D., Williamson,P. and Schlegel,R.A.
  TITLE     Differential expression of putative transbilayer amphipath
            transporters
  JOURNAL   Physiol Genomics 1 (3), 139-150 (1999)
   PUBMED   11015572
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC137585.3, AK036959.1, AC102268.11, BQ714379.1 and AA242626.1.
            
            On May 9, 2006 this sequence version replaced NM_001001488.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK157316.1, AY506548.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-301               AC137585.3         84130-84430         c
            302-1710            AK036959.1         223-1631
            1711-1752           AC102268.11        101795-101836       c
            1753-1796           AC102268.11        99622-99665         c
            1797-1953           AC102268.11        99032-99188         c
            1954-2523           AK036959.1         1876-2445
            2524-2741           BQ714379.1         373-590
            2742-3030           AC102268.11        82569-82857         c
            3031-3254           AC102268.11        78730-78953         c
            3255-3338           AC102268.11        77737-77820         c
            3339-3584           AC102268.11        76483-76728         c
            3585-4017           AA242626.1         40-472
            4018-6440           AC102268.11        66431-68853         c
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 37.49 cM"
     gene            1..6440
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /note="ATPase, class I, type 8B, member 1"
                     /db_xref="GeneID:54670"
                     /db_xref="MGI:MGI:1859665"
     exon            1..301
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            302..504
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     CDS             324..4079
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /EC_number="7.6.2.1"
                     /note="probable phospholipid-transporting ATPase IC;
                     aminophospholipid translocase; familial intrahepatic
                     cholestasis type I; ATPase 8B1, p type; ATPase B1, class
                     I; P4-ATPase flippase complex alpha subunit ATP8B1"
                     /codon_start=1
                     /product="phospholipid-transporting ATPase IC"
                     /protein_id="NP_001001488.2"
                     /db_xref="CCDS:CCDS29304.1"
                     /db_xref="GeneID:54670"
                     /db_xref="MGI:MGI:1859665"
                     /translation="MSTERDSETTFDEESQPNDEVVPYSDDETEDELEDQGSTVEPEQ
                     NRVNREAEKKRETFRKDCTWQVKANDRKFHEQPHFMNTKFFCIKESKYASNAIKTYKY
                     NGFTFLPMNLFEQFKRAANFYFLILLILQAIPQISTLAWYTTLVPLLLVLGITAIKDL
                     VDDVARHKMDKEINNRTCEVIKDGRFKIIKWKDIQVGDVIRLKKNDFIPADILLLSSS
                     EPNSLCYVETAELDGETNLKFKMALEITDQYLQIEDNLATFDGFIECEEPNNRLDKFT
                     GTLFWKNQSFPLDADKILLRGCVIRNTDVCHGLVIFAGADTKIMKNSGKTRFKRTKID
                     YLMNYMVYTIFIVLILVSAGLAIGHAYWEAQVGNYSWYLYDGENATPSYRGFLNFWGY
                     IIVLNTMVPISLYVSVEVIRLGQSHFINWDLQMYYAEKDTPAKARTTTLNEQLGQIHY
                     IFSDKTGTLTQNIMTFKKCCINGTIYGDHRDASQHSHSKIELVDFSWNTFADGKLAFY
                     DHYLIEQIQSGKEPEVRQFFFLLSICHTVMVDRIDGQINYQAASPDEGALVNAARNFG
                     FAFLARTQNTITVSELGSERTYNVLAILDFNSDRKRMSIIVRTPEGSIRLYCKGADTV
                     IYERLHRMNPTKQETQDALDIFASETLRTLCLCYKEIEEKEFTEWNNKFMAASVASSN
                     RDEALDKVYEEIEKDLILLGATAIEDKLQDGVPETISKLAKADIKIWVLTGDKKETAE
                     NIGFACELLTEDTTICYGEDINSLLHTRMENQRNRGGVSAKFAPPVYEPFFPPGENRA
                     LIITGSWLNEILLEKKTKRSKILKLKFPRTEEERRMRSQSRRRLEEKKEQRQKNFVDL
                     ACECSAVICCRVTPKQKAMVVDLVKRYKKAITLAIGDGANDVNMIKTAHIGVGISGQE
                     GMQAVMSSDYSFAQFRYLQRLLLVHGRWSYIRMCKFLRYFFYKNFAFTLVHFWYSFFN
                     GYSAQTAYEDWFITLYNVLYSSLPVLLMGLLDQDVSDKLSLRFPGLYVVGQRDLLFNY
                     KRFFVSLLHGVLTSMVLFFIPLGAYLQTVGQDGEAPSDYQSFAVTVASALVITVNFQI
                     GLDTSYWTFVNAFSIFGSIALYFGIMFDFHSAGIHVLFPSAFQFTGTASNALRQPYIW
                     LTIILTVAVCLLPVVAIRFLSMTIWPSESDKIQKHRKRLKAEEQWKRRQSVFRRGVSS
                     RRSAYAFSHQRGYADLISSGRSIRKKRSPLDAIIADGTAEYRRTVES"
     exon            505..602
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            603..716
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            717..815
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            816..877
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            878..950
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1021
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1104
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1263
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1352
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1543
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1752
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1796
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1953
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2142
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2255
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2420
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2532
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2608
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            2609..2741
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            2742..3030
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            3031..3254
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            3255..3338
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            3339..3584
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            3585..3723
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            3724..3854
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     exon            3855..6440
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6406..6411
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /note="hexamer: AATAAA"
     polyA_site      6426
                     /gene="Atp8b1"
                     /gene_synonym="FIC1; Ic"
                     /note="major polyA site"
ORIGIN      
        1 gagaaaccag tttgtccggt cgggcagagc ggagggaaaa gggctgccgc ctaggaaggg
       61 cgcacggacg cggggcgggg ggaaggcggg gagcccttgg cgagccacga gagcctgagg
      121 agtcgcggcc aactggacag gcttcccggg gcccccggcc agcccctgtg tgccatgcag
      181 cgctagcgca gccgcagcgc cggggccggg cgggaggtgg ccaggtgcgc accagcctcg
      241 gacaagcctc ggagcgccgc gcaccccgcg gatgcccaag ccggggccca gtgagcacta
      301 ggtagttcca atttgccagc agaatgagca cagaaagaga ctcggaaaca acatttgatg
      361 aggagtctca gcccaacgat gaagtggtcc cctacagtga tgatgaaact gaagacgaac
      421 ttgaagatca ggggtccacg gttgaaccag aacagaatcg tgtcaacaga gaagctgaga
      481 agaagagaga gacattccgg aaagattgca cgtggcaagt caaagcaaat gaccgaaagt
      541 tccacgaaca acctcatttc atgaacacca agttcttttg tattaaggag agcaaatatg
      601 cgagtaatgc aattaaaaca tacaagtaca atggatttac ctttctaccc atgaatttgt
      661 ttgagcagtt taagagggca gccaacttct atttcctcat cctcctgatc ctgcaggcaa
      721 ttcctcaaat ctccaccctg gcatggtata ccacacttgt acctctgctt ttggtgctgg
      781 gcatcacggc catcaaagac ctggtagatg atgtggctcg tcataaaatg gacaaggaaa
      841 tcaacaatag gacgtgtgaa gtcattaagg atggcaggtt caaaatcatc aagtggaaag
      901 atatccaggt tggagatgtc atccgcctga agaagaatga ttttattcca gctgacatcc
      961 tgctgttgtc cagttcagag cctaacagcc tctgctatgt agaaacggct gagctcgacg
     1021 gagaaaccaa tttgaagttc aaaatggccc tggaaatcac ggaccaatat ctccaaatag
     1081 aagataactt ggcaacattt gatggtttca tcgaatgtga agaaccaaac aatcggttag
     1141 acaagttcac aggaacgctg ttttggaaga accaaagctt tcctttggat gctgataaaa
     1201 tcctgttacg aggttgtgtg ataaggaaca ccgatgtctg ccatggcctg gtcatttttg
     1261 caggtgctga cacaaagata atgaagaata gtgggaaaac cagatttaaa agaaccaaaa
     1321 ttgattactt gatgaactac atggtgtaca cgatctttat tgttctcatc ctggtttctg
     1381 ccggcctcgc cattggccac gcctattggg aagcccaagt tggcaattat tcctggtacc
     1441 tctatgatgg agaaaatgcc acaccgtcct accggggatt cctgaatttc tggggctaca
     1501 tcattgtatt gaacaccatg gtgcccattt ctctctatgt cagtgtggaa gtgatccgtc
     1561 tgggacagag tcatttcatc aactgggacc tgcagatgta ctatgctgag aaggacactc
     1621 ctgccaaggc gaggaccacc accttgaatg agcagctagg gcagattcat tacatcttct
     1681 ctgataagac ggggacactc acgcaaaata tcatgacctt caaaaagtgc tgcattaacg
     1741 ggactatcta tggagaccat cgagatgctt ctcaacacag ccacagcaaa atcgagctag
     1801 tggactttag ctggaataca tttgccgatg gaaagcttgc attttatgac cattacctta
     1861 tcgagcaaat acagtcaggg aaagagccag aagtacgaca gttcttcttt ttgctttcga
     1921 tttgccatac ggtcatggtg gacagaattg atggtcagat caactaccag gcggcttctc
     1981 ctgatgaagg cgccctggta aacgccgcca ggaacttcgg ctttgccttt ctcgcccgga
     2041 cgcagaacac catcaccgtc agcgaactgg gctcagagag gacctacaac gtccttgcca
     2101 ttttggattt caacagcgac cgaaagcgga tgtctatcat cgtgaggacc ccagaaggta
     2161 gtatccggct gtactgtaaa ggtgctgaca ccgtgattta tgaacggctc catcggatga
     2221 atcctacgaa gcaagaaaca caggatgccc tggatatctt tgcaagtgag actctcagga
     2281 ccctgtgcct gtgctacaag gaaattgaag aaaaagaatt tacagaatgg aataataagt
     2341 ttatggccgc cagcgtggcc tcaagcaacc gagacgaagc gttggataaa gtgtacgagg
     2401 agattgagaa agacttgatt ctcttgggag ctactgctat tgaagacaag ctacaggatg
     2461 gagtcccaga aaccatttca aaacttgcaa aagctgacat taagatctgg gtactcaccg
     2521 gagacaaaaa ggaaactgca gaaaacatag gttttgcctg tgaactcttg acggaagaca
     2581 ctactatctg ctacggagag gacataaact ctcttcttca caccaggatg gagaatcaga
     2641 gaaacagagg tggagtctct gcgaaatttg cacctcctgt gtatgaaccc ttttttccac
     2701 ctggagaaaa ccgtgcctta atcatcactg gctcctggct gaatgaaatc cttctagaga
     2761 agaagaccaa gagaagtaag atcctgaagc tgaagttccc aaggacagag gaggagaggc
     2821 ggatgcggag ccagagccgg agacggctgg aagagaagaa agagcagcgg caaaagaact
     2881 tcgtggacct ggcctgcgag tgcagcgccg tcatctgctg ccgcgtcacg cccaagcaga
     2941 aggccatggt ggtggacctg gtgaagaggt acaagaaagc catcaccctg gccatcggag
     3001 acggggccaa tgatgtcaac atgatcaaaa ctgcccacat tggtgttgga ataagtggac
     3061 aagaagggat gcaagctgtc atgtccagtg actattcttt tgctcagttc agatatctgc
     3121 agcggttgct gctggtccac ggccggtggt cttacataag gatgtgcaag ttcctacgat
     3181 acttctttta caagaacttt gcctttactt tagttcattt ctggtactcc ttcttcaatg
     3241 gctactcagc ccagacggcg tatgaggatt ggtttatcac cctctacaac gtgctgtact
     3301 ccagtttgcc tgtgctcctc atggggctgc tggatcagga cgtgagtgac aagctgagcc
     3361 tccgattccc tgggctgtat gtagtggggc agagggactt gctgttcaac tataagagat
     3421 tctttgtcag tttgctgcat ggggtcttaa cctcaatggt cctcttcttc ataccgcttg
     3481 gtgcttatct gcaaacagtg ggacaggatg gggaggcgcc ttcagactac cagtccttcg
     3541 ccgtcacggt ggcctccgct ctagtgataa cggtcaactt ccagattggc ttggatactt
     3601 catactggac ttttgtgaat gccttttcaa ttttcggcag catcgcactg tacttcggca
     3661 tcatgtttga tttccacagt gctgggatac acgttctctt tccgtccgca tttcagttca
     3721 caggtacagc ttccaatgcc ctgagacagc catacatctg gctgaccatc atcctgacgg
     3781 ttgccgtgtg cttactacct gtcgtcgcca tccgtttcct gtccatgacc atttggccct
     3841 cagaaagtga taagatccag aagcatcgaa agcgattgaa ggctgaagag cagtggaaac
     3901 ggcggcagag cgtgttccgg aggggagtat cttccaggcg ctcagcctat gccttttcgc
     3961 atcagagagg ctacgcagac ctcatctcct ctggccgcag catccgcaag aagcgctcgc
     4021 ccctggatgc catcatcgcg gatggcacgg ctgagtaccg gcgcacagtg gagagctgat
     4081 tggcattgct ctgctgagag accggagcgc tgggaagacg ttttgttttt gtttttgttt
     4141 ttttctttaa tgaacgactc aggacctttt taaaaacaca aacaaactga agttgtcact
     4201 gatgacaata accttttcaa taaacttcta ggattgtgct gttaagcaat cgcaaagcca
     4261 agacaccacc tgttattcag cggattgtgt ctgctgagat tcaaagggaa cttttttttt
     4321 tttaatcctg gcctaatgga agacggcaga gtttagaaaa gtctcccatt tcagctggaa
     4381 ggacactact acactacaaa gttacaatta cactcttctc tctgtggcct caaatgtgga
     4441 tttctcagat gcactccccc ctctgcttgt catctttcaa aagaagaaaa cagaaaatct
     4501 gtttttaatg tatgggtgca gtaacaaagg gttgctaatg tttatcacct gacaggaagt
     4561 ggtttaatga ctttttcaga ccttgttggg gctccctgtt tctttctacc aactttcgaa
     4621 atttgaaaca agtttccttg attgaatgat ttaggtgcaa catttcactc tggttttgat
     4681 tttactacca agctaaataa tgtatcccat gtttttttac tatttggtat ttctctgtac
     4741 taccaagcgg tttgactctc tactttaatt ttaaattttt aaaaatgaca aaaaaatatt
     4801 ttttaaaagc tgaggacatt atcaactagt attaagtacc atttagataa gagaaataaa
     4861 ttgtggcatc tcttgcaccg taagggatca tgataataga tcatgataat ggaatgtgtg
     4921 tttcagaaat gctagacgaa agggatttaa atgttttcaa caagaagtga taactgagaa
     4981 atggaaaaaa tgtgatgtca actttataca atatacatgt gctgaggaac atggcaggcc
     5041 accttgtggc ttggtggcaa agctggagtc ttaggtccca tagcagtata aaatggaaga
     5101 aacatcacgt gatgtcctat aaatagacac aattatggat cacctaaaat tgactagaaa
     5161 gggacaggac tatgcctact gaaattttcc ttgtggtttc ctttggaaga caacactatt
     5221 tagctcttcc cagtcactgg atggactggg aggttgcggg gtgggagggg agaaacaaga
     5281 gccttgactc ttaagtgatg ggaacattgt tactactgcc cacaacagag gagaataata
     5341 catacaaagg tgtatccaaa ctgctgccgg tggccacatc caggcaagga gaactatgaa
     5401 tatagcccaa tacaaaaatg gttaaaatta cttaaaacac taggaaattg tgtgtgtata
     5461 tgcccatgtg caactccatt gcttggtctt gagtgtgagc tttcctggta gtttgtccca
     5521 ctgtcaggag catagactta cctggtaggg agtctgtgga gaactttccc ctgtgtttcc
     5581 tggaagtaaa cgagaggtct actgttcaga acagataagt ttaaacaaac aaacaaaaag
     5641 cccagagggc atcagtgata cctttgggaa tattttgcag agttctctgt tgaaaagaaa
     5701 ctgcttgtgt cccccttccc agaaagtatt ttatacaagt cttttggagc ctcgctcttg
     5761 catctgtctt atgtctaaga caaaaagaac agttttttcg tttcgttttt gttttttatt
     5821 ttggaaaaaa cattaggtga ttaaggaaaa tatccttgaa aaggtaactt atttttcccc
     5881 ccaggggatt tttctcattt tatttctttc acagaacagt ccccctgctc tactgaaggc
     5941 ctctgtgggt tgtagtaggt aactttcttt ccccttgcaa atgttcatag cagtctgtga
     6001 attcccaaga ggaaatcatg tgtagttcat cttctataca caggacagga aaacattaaa
     6061 ttgtactttt ataatgcatt gtctagatat gatgaaagaa tgtatgtcta tctcctagag
     6121 gtaaatttct gaaagtttac aactttacca tgtaaataaa aagaattgta tgttttcctt
     6181 tttttatgga aaatttagct tgtgtaattt tgtcaatttc aagtttcgct tttgtttttg
     6241 ttttttttgt tttgtttttt gtttttgaat ggtgggaagg gttggcaatg caaatgtgtc
     6301 aaagacttac actgctgggt gcaatattaa taatttaaaa tgcaaatcat aaattatttc
     6361 tatatgctgt aaatctgaga tttaatgtat atttttgttt aagaaaataa atgctttagt
     6421 aaaatatttg gaacatgtca
//
