LOCUS       NM_001284345            8176 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus ATPase phospholipid transporting 8A1 (Atp8a1),
            transcript variant 3, mRNA.
ACCESSION   NM_001284345
VERSION     NM_001284345.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Guo,Y., Xiang,P., Sun,X., Liu,W., Zhou,J., Yin,B., Hou,L.,
            Qiang,B., Li,H., Shu,P. and Peng,X.
  TITLE     Docking protein 6 (DOK6) selectively docks the neurotrophic
            signaling transduction to restrain peripheral neuropathy
  JOURNAL   Signal Transduct Target Ther 9 (1), 32 (2024)
   PUBMED   38351062
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8176)
  AUTHORS   Xu,H., Oses-Prieto,J.A., Khvotchev,M., Jain,S., Liang,J.,
            Burlingame,A. and Edwards,R.H.
  TITLE     Adaptor protein AP-3 produces synaptic vesicles that release at
            high frequency by recruiting phospholipid flippase ATP8A1
  JOURNAL   Nat Neurosci 26 (10), 1685-1700 (2023)
   PUBMED   37723322
  REMARK    GeneRIF: Adaptor protein AP-3 produces synaptic vesicles that
            release at high frequency by recruiting phospholipid flippase
            ATP8A1.
REFERENCE   4  (bases 1 to 8176)
  AUTHORS   Zheng,L., Pan,C., Tian,W., Liang,C., Feng,Y., He,W., Yang,Z.,
            Wang,B., Qiu,Q., Li,N., Sun,Y., Qiu,H., Sample,K.M., Zhou,L.,
            Zhu,X. and Hu,Y.
  TITLE     Atp8a1 deletion increases the proliferative activity of
            hematopoietic stem cells by impairing PTEN function
  JOURNAL   Cell Oncol (Dordr) 46 (4), 1069-1083 (2023)
   PUBMED   36930333
  REMARK    GeneRIF: Atp8a1 deletion increases the proliferative activity of
            hematopoietic stem cells by impairing PTEN function.
REFERENCE   5  (bases 1 to 8176)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8176)
  AUTHORS   Wang,L., Beserra,C. and Garbers,D.L.
  TITLE     A novel aminophospholipid transporter exclusively expressed in
            spermatozoa is required for membrane lipid asymmetry and normal
            fertilization
  JOURNAL   Dev Biol 267 (1), 203-215 (2004)
   PUBMED   14975727
  REMARK    GeneRIF: role in normal phospholipid distribution in the bilayer,
            and for normal binding, penetration, and signaling by the zona
            pellucida
REFERENCE   7  (bases 1 to 8176)
  AUTHORS   Ding,J., Wu,Z., Crider,B.P., Ma,Y., Li,X., Slaughter,C., Gong,L.
            and Xie,X.S.
  TITLE     Identification and functional expression of four isoforms of ATPase
            II, the putative aminophospholipid translocase. Effect of isoform
            variation on the ATPase activity and phospholipid specificity
  JOURNAL   J Biol Chem 275 (30), 23378-23386 (2000)
   PUBMED   10801890
REFERENCE   8  (bases 1 to 8176)
  AUTHORS   Wang,Y., Saigoh,K., Osaka,H., Yamanishi,T., Suh,J., Kiyosawa,H.,
            Sakai,Y., Wakana,S. and Wada,K.
  TITLE     YAC/BAC-based physical and transcript mapping around the gracile
            axonal dystrophy (gad) locus identifies Uchl1, Pmx2b, Atp3a2, and
            Hip2 genes
  JOURNAL   Genomics 66 (3), 333-336 (2000)
   PUBMED   10873389
REFERENCE   9  (bases 1 to 8176)
  AUTHORS   Halleck,M.S., Lawler,J.F. Jr., Blackshaw,S., Gao,L., Nagarajan,P.,
            Hacker,C., Pyle,S., Newman,J.T., Nakanishi,Y., Ando,H.,
            Weinstock,D., Williamson,P. and Schlegel,R.A.
  TITLE     Differential expression of putative transbilayer amphipath
            transporters
  JOURNAL   Physiol Genomics 1 (3), 139-150 (1999)
   PUBMED   11015572
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 8176)
  AUTHORS   Halleck,M.S., Pradhan,D., Blackman,C., Berkes,C., Williamson,P. and
            Schlegel,R.A.
  TITLE     Multiple members of a third subfamily of P-type ATPases identified
            by genomic sequences and ESTs
  JOURNAL   Genome Res 8 (4), 354-361 (1998)
   PUBMED   9548971
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK151709.1, AK045367.1, AK141559.1, BC094235.1 and AC123662.13.
            
            Transcript Variant: This variant (3) lacks an alternate exon and
            contains a different alternate exon in the 5' coding region,
            compared to variant 1. It encodes isoform c, which is of the same
            size but lacks an internal segment, compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC129872.1, SRR10662773.365692.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AK151709.1         2-20
            20-671              AK045367.1         1-652
            672-745             AK151709.1         673-746
            746-4577            AK141559.1         681-4512
            4578-6888           BC094235.1         3283-5593
            6889-8176           AC123662.13        48441-49728
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 36.15 cM"
     gene            1..8176
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /note="ATPase phospholipid transporting 8A1"
                     /db_xref="GeneID:11980"
                     /db_xref="MGI:MGI:1330848"
     exon            1..270
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..3716
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /EC_number="7.6.2.1"
                     /note="isoform c is encoded by transcript variant 3;
                     probable phospholipid-transporting ATPase IA; chromaffin
                     granule ATPase II; ATPase class I type 8A member 1; ATPase
                     8A1, p type; ATPase 8A1, aminophospholipid transporter
                     (APLT), class I; P4-ATPase flippase complex alpha subunit
                     ATP8A1; ATPase, aminophospholipid transporter (APLT),
                     class I, type 8A, member 1"
                     /codon_start=1
                     /product="phospholipid-transporting ATPase IA isoform c"
                     /protein_id="NP_001271274.1"
                     /db_xref="CCDS:CCDS80295.1"
                     /db_xref="GeneID:11980"
                     /db_xref="MGI:MGI:1330848"
                     /translation="MPTMRRTVSEIRSRAEGYEKTDDVSEKTSLADQEEVRTIFINQP
                     QLTKFCNNHVSTAKYNVITFLPRFLYSQFRRAANSFFLFIALLQQIPDVSPTGRYTTL
                     VPLLFILAVAAIKEIIEDIKRHKADNAVNKKQTQVLRNGAWEIVHWEKVNVGDIVIIK
                     GKEYIPADTVLLSSSEPQAMCYIETSNLDGETNLKIRQGLPATSDIKDIDSLMRISGR
                     IECESPNRHLYDFVGNIRLDGHGTVPLGADQILLRGAQLRNTQWVHGIVVYTGHDTKL
                     MQNSTSPPLKLSNVERITNVQILILFCILIAMSLVCSVGSAIWNRRHSGKDWYLHLHY
                     GGASNFGLNFLTFIILFNNLIPISLLVTLEVVKFTQAYFINWDLDMHYEPTDTAAMAR
                     TSNLNEELGQVKYIFSDKTGTLTCNVMQFKKCTIAGVAYGHVPEPEDYGCSPDEWQSS
                     QFGDEKTFNDPSLLDNLQNNHPTAPIICEFLTMMAVCHTAVPEREGDKIIYQAASPDE
                     GALVRAAKQLNFVFTGRTPDSVIIDSLGQEERYELLNVLEFTSARKRMSVVVRTPSGK
                     LRLYCKGADTVIYERLAETSKYKEITLKHLEQFATEGLRTLCFAVAEISESDFEEWRA
                     VYHRASTSVQNRLLKLEESYELIEKNLQLLGATAIEDKLQDQVPETIETLMKADIKIW
                     ILTGDKQETAINIGHSCRLLKRNMGMIVINEGSLDGTRETLSRHCTTLGDALRKENDF
                     ALIIDGKTLKYALTFGVRQYFLDLALSCKAVICCRVSPLQKSEVVEMVKKQVKVITLA
                     IGDGANDVSMIQTAHVGVGISGNEGLQAANSSDYSIAQFKYLKNLLMVHGAWNYNRVS
                     KCILYCFYKNIVLYIIEIWFAFVNGFSGQILFERWCIGLYNVMFTAMPPLTLGIFERS
                     CRKENMLKYPELYKTSQNALDFNTKVFWVHCLNGLFHSVILFWFPLKALQYGTVFGNG
                     KTSDYLLLGNFVYTFVVITVCLKAGLETSYWTWFSHIAIWGSIALWVVFFGIYSSLWP
                     AVPMAPDMSGEAAMLFSSGVFWVGLLSIPVASLLLDVLYKVIKRTAFKTLVDEVQELE
                     AKSQDPGAVVLGKSLTERAQLLKNVFKKNHVNLYRSESLQQNLLHGYAFSQDENGIVS
                     QSEVIRAYDTTKQRPDEW"
     exon            271..385
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            386..485
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            486..584
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            585..630
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            631..671
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            672..745
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            746..815
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            816..943
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1055
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1221
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1349
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1427
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1516
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1561
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1634
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1740
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1823
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1873
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..1943
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2028
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2168
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2307
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2372
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2545
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2729
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2840
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2915
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            2916..3038
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3117
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            3118..3179
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            3180..3236
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            3237..3344
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            3345..3433
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            3434..3526
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            3527..3618
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     exon            3619..8176
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7132..7137
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /note="hexamer: AATAAA"
     polyA_site      7154
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /note="major polyA site"
     regulatory      8141..8146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
                     /note="hexamer: ATTAAA"
     polyA_site      8158
                     /gene="Atp8a1"
                     /gene_synonym="APLT; Atp3a2; B230107D19Rik; ClassI"
ORIGIN      
        1 actgcgggga gacctaggcg gctctgcgga cgcagctcct tcgccgcctt ccccctcccg
       61 tccagtgccc aggcggctcc tggcggcgac gctgccctgg gtgggaggcg cggccccgcg
      121 gcagctgagc cctctgcgcg gcgcagccag ctctcccgcc cgcgcggcgc cgtgacaggt
      181 gcagggtccc cgcccgagac ccacctgcag gggctgtcga gatgccgacc atgcggagga
      241 cagtgtcgga gatccgctcg cgcgcggaag gttatgagaa gacagatgat gtttcagaga
      301 agacctcgct ggcagatcag gaggaagtga gaaccatctt catcaaccag cctcagctga
      361 caaaattctg caacaaccac gtcagtactg caaaatacaa cgtgatcaca ttccttccga
      421 ggttcctcta ttctcagttc cgaagagcgg ctaattcgtt ctttctcttt attgccctgc
      481 tccagcaaat tcctgatgtg tcgccgaccg gtcgttacac aacactggtt cctctcttat
      541 ttattttagc tgtggctgct attaaagaga taatagaaga tattaaacga cacaaggctg
      601 ataatgctgt gaacaagaaa cagacacaag ttttgagaaa tggtgcatgg gaaattgttc
      661 actgggaaaa ggtaaatgtt ggagatatag ttataataaa aggcaaagag tatatacctg
      721 ctgacactgt ccttctctcg tcaagtgagc cccaggccat gtgctacatc gagacatcca
      781 acttagatgg tgaaacaaac ttgaaaatta gacaaggctt accggcaaca tcggatatca
      841 aagacattga cagtttgatg agaatttctg gcagaatcga gtgtgaaagt ccaaaccgac
      901 atctctacga ttttgttggg aacataaggc ttgatggcca tggcaccgtt cccctggggg
      961 cagaccagat ccttctccga ggagctcagt taagaaatac ccagtgggtt catgggatag
     1021 ttgtctacac tggccacgac accaagctga tgcagaattc cacaagccca ccacttaaac
     1081 tctccaatgt ggaacggatt acaaatgtac aaattctgat tttattttgc atcttaattg
     1141 ccatgtctct tgtgtgttct gtgggctcag ccatttggaa ccgaaggcat tccgggaaag
     1201 actggtacct ccatctacac tatggtggcg ctagtaactt tggactgaac ttcctgactt
     1261 tcattatcct tttcaacaac ctcattccca tcagcttgct ggtcacatta gaagtggtga
     1321 agtttactca ggcatacttc ataaattggg atcttgacat gcattatgag cccacagaca
     1381 ccgcagcaat ggctcggaca tctaatctga atgaggaact tggccaggtt aaatacatat
     1441 tttctgacaa aactgggacc ctgacatgca atgtgatgca gttcaagaag tgcaccatcg
     1501 cgggcgtggc ctatggccat gtccctgaac ctgaggatta tgggtgctcg cccgatgaat
     1561 ggcagagctc acagtttgga gatgaaaaaa cctttaatga cccgtcgttg ctggacaatc
     1621 tccagaataa ccacccaacc gcacctatca tctgtgaatt tctcacaatg atggccgtct
     1681 gccacacagc tgtaccggag agagaagggg acaagatcat ttatcaggct gcatcgccag
     1741 atgagggtgc actggtcaga gccgccaagc agttgaattt tgtcttcact ggaagaactc
     1801 ctgactctgt catcatagat tcactggggc aggaagaaag atatgaattg ctcaatgtcc
     1861 tagagttcac cagtgctagg aagaggatgt cggtggtggt tcgcactcca tccgggaagt
     1921 tacggctcta ctgcaaagga gctgacacag taatttatga acgacttgca gagacctcaa
     1981 agtacaaaga aatcacccta aaacacttgg aacagtttgc tacagaaggg ctgaggactt
     2041 tgtgttttgc tgtggctgag atttctgaga gcgacttcga ggagtggcgg gccgtctacc
     2101 accgcgcatc cacgtcggta cagaacaggc tgctgaagct ggaggagagc tacgaactaa
     2161 ttgaaaagaa tcttcagcta cttggagcta cagccattga ggataaattg caggaccaag
     2221 tgcctgaaac catagaaacg ctaatgaagg ctgacatcaa aatatggatc cttactgggg
     2281 acaagcaaga aactgccatt aatattggac actcctgtag actcctcaag aggaacatgg
     2341 gaatgatcgt gataaacgag ggctctcttg acggcacgag ggaaactctc agccgccact
     2401 gcaccaccct gggagatgct cttcggaagg aaaatgattt tgctcttata attgatggga
     2461 agaccctcaa atatgcctta acctttggcg tccggcagta tttcctggac ttagctctgt
     2521 cctgcaaagc tgtcatttgc tgcagggttt ctcctcttca gaagtccgag gttgtcgaga
     2581 tggttaagaa acaagtcaaa gtcatcacac ttgccatcgg ggatggagca aatgacgtca
     2641 gcatgatcca gacggcccat gtgggtgttg ggataagcgg caatgaaggt ttgcaggcag
     2701 ccaactcttc agattactcc atcgctcagt tcaaatattt gaagaatttg ttgatggttc
     2761 acggtgcctg gaattacaat agagtctcca agtgtatcct gtactgcttc tacaagaaca
     2821 tcgtgctcta catcatcgag atctggtttg cctttgtcaa cggcttttct ggacagatcc
     2881 tctttgaaag atggtgtata gggctttata atgtgatgtt cacagcgatg cctcccttga
     2941 cacttggaat atttgagaga tcgtgcagaa aggagaacat gttgaagtat cctgagttgt
     3001 acaaaacatc ccagaatgct ctggacttca ataccaaggt tttctgggtt cattgtttga
     3061 atggcctctt ccactccgtt attctgtttt ggttcccact gaaagccctg cagtatggca
     3121 cggtatttgg aaatgggaaa acctcagatt acctgcttct gggaaacttt gtttatactt
     3181 ttgtagtgat aactgtgtgc ttgaaagctg gactggaaac ctcctattgg acatggttca
     3241 gccacatcgc catctggggc agcatcgcgc tctgggtggt gttcttcggg atctactcat
     3301 ctctgtggcc tgccgtgccc atggcccctg acatgtccgg agaggcagcc atgctcttca
     3361 gctctggagt cttctgggtg ggcttgctct ccatccctgt ggcgtccttg cttctggatg
     3421 tgctgtacaa agtcatcaag aggacggcct ttaaaacctt agtggatgaa gttcaggagc
     3481 ttgaggcgaa atctcaagac ccgggcgcag tcgtacttgg aaagagcctc acggagagag
     3541 cgcagctgct caagaacgtc tttaagaaga accacgtgaa tctgtaccgc tccgagtccc
     3601 tgcagcagaa cctgcttcac ggctatgctt tctctcaaga tgaaaacggc atcgtctcac
     3661 agtctgaagt catcagagcc tatgacacca cgaaacagag gcccgatgag tggtgattgg
     3721 caggcctaag ccaccggccc ccccgctgtg tctctgagga gaaccatcac agtctaaggt
     3781 catcactgta acctgctgac caattccagt ctggtccatg gggagaaaaa gtggatctga
     3841 gctcatctca cagatgaatt tcaaggacat agcgctatgt gactactgta ataccattgt
     3901 ccttggatgt cctcaaagta cctgctaagg ctttgtaaat cgatattgga aattaccttg
     3961 tatcttgcca cagtgctttc tcaaaatggg gacaggtcag tgttcacaag ccgtccctct
     4021 ggggctgtaa ctatcgttca ttggctcaac caccagtcaa tcaaccattc aaaagaaaca
     4081 acaactcaga agtgatgtta agggaaggac tctcaatatc cggactatcg ggatatactg
     4141 aaataagaca gctcactcta ctgagcattt ctaaacaagg cattaacatg tgtttgtgtc
     4201 aaagagtgtc ttgctaagtg tttgccaaag acgttcagtg tgtgcatttc tcattcagta
     4261 gtcatctgcc cagaaattta aaagaaagtt tatttcatgg tatctctgta ccatctgcat
     4321 gcttgacttt tcaaacatga gcgtgactta aaattttgag catttccagg cgtttgctac
     4381 taaaatctgt gatgttgcac ctttggcctt acaactgctt ttctgtcgtt tgtcttcttt
     4441 tatttgttcc agttggaggg acgtgtgtcc gtgtgaccgt tgttatgatg ctgacttttt
     4501 ttttcctttt ttaattgtac tgtctctcat ttctgatgaa cgttagactc ctttagatgt
     4561 tcctttaaaa aaaaaaatct ggcttttgcc gtgtattgag tggctacgtc ttcagtcttt
     4621 cccctcctaa ggaaattacc cttacatatt tatgtgggat ttccaaagac ctttgggata
     4681 atccgtgaga tagtgcatgg gctttttgtc atgtacatag gctcagcttt tgtcctgtgc
     4741 tgtttgttct tcactgtgcg ctcccactgg cgatgatctg tgcagtacag ttggtctcat
     4801 ggcgactacc cgtatgctta agcctgaaaa ttatttcaca gttcactcag ccgacaactc
     4861 ccaaactgag ttgcatactg cttatcgaca cgagagagat aaggggcaca cttccctcgt
     4921 tttaaggaaa tacctatttt actctagcct aacacctccc agtcaatgaa tacttaccat
     4981 cataagtagg gttcactgat acgtttttca aacatgtgtt cgcgctgatc agtttggcat
     5041 ctccatgaat tcgcctgaaa aaaaaaaaaa gcctattttg ctacctacag taaatggtga
     5101 aggggctgtt taaaaaccac acccagtttt ctacagtttt tatggtattt ttttcatctt
     5161 aaaagataaa aaaaaagggg ggagttctag ttttcagata cacttcagag atcgaaacaa
     5221 actcttctgc cttctattca aaagcctgtt tgcctttttg tggattacca gcaaaataga
     5281 ccttcaaacg tgtgcaagga ctagctttga gaaggggtgt tttctccata ttaccccatt
     5341 aaatcatttc atttcccctt cccccctttt cttggtctga atttaaaatt aggctgtgtc
     5401 ttccttctct aaagctgtag ctagtttcca tcgtgtctcc atactccctg caaagaagat
     5461 aaggttctaa aaattcacgt aattggaaag ggagcacagt ggacacactt cacatctcaa
     5521 atggtcccgt ttggagctta gatcagccac acgagcatca aagcatctag cagaaccaca
     5581 caacatagaa tctaagctga tcttccagac tgtgaggctg caggcttagc tgtccaccag
     5641 ctgaatctgg agagtgggtt gttctcagcc ttctgggggt gaccattgag gtcattcccc
     5701 ccaaaatgac tggttatcca agaaaggctt gcttcccttg gccccgccat cagaggtatc
     5761 tgtctccaga ttcctcgcct cagaacattg tcaatgagaa tgggatcttc tgacctctgc
     5821 ctggaaatct aaagtcatct ttttatcctg aatcataaat tattttgatg gaactcttcc
     5881 acatgagcac actcagttat acaacatacc ctgaaaacga gactctatat gccttttgac
     5941 catggatcct gtgtttgaat gacaggcatt aatttggtct ctctgttctt aagttgggaa
     6001 gaacacagag agtggccatt tgggtaaaac ttgaaatagg gacttgctat agcagtgagt
     6061 ttacagagac ttaatactct caagtttcta acctttgtca gctggggaaa aggcacatgg
     6121 ttttaccatg gatttgaatg ggtgcagtca aagtgacttt aagcctatgt tttctgaaat
     6181 aaacaacaca gaactccaat ttaaaagtcc cctgtagggg actctggtaa atgctgacat
     6241 gagtgctttg taacgtttac aatccagaaa agcggggcgg tgaatcgttg gtgttgggtt
     6301 gggatatcgc acagtagtgg agagcataga ctttctgtgt gttagatata cacctaacaa
     6361 ttcactgctg tatcacgcct ataagatgca ctattggtga aggtattagc aggaaagcag
     6421 tgttcttggg aacgtaggcc ttttaacatg gtaaatgtgc ctcttggctg gttctccact
     6481 cagtgctccc tgggagccgg gagaacttgc tggattgctt ctttgtcacc ctggcaagtg
     6541 aagggatact gccaaataag ggagccacgg actgtgttgt cattaaagag agtcagtgag
     6601 ttggtttcct ttccatcagg aaaaatggat tctgtaaaga gtcagggcgt tgtctcagca
     6661 tttgatgata tattttaatt tttcagttca gtatcccaga tagctggtgt gatgctcaca
     6721 ataggttagt ctgttctata ctcagtgtca cactcataac ctgtgattga aagctgccct
     6781 ttcagaaaac gaatgctagt cagaaggcaa tgggttggcg tcctttttaa tcagtgtgta
     6841 actattataa agctacctgt tcatcataca cttggttttt tttttttttc tgatgtttcc
     6901 tggtcttaaa acttgtatca taaaatgaat ctctagtcta ataattaagc tacaaaattt
     6961 attaaggaaa tgccccactc attcacacca tggtgagcaa gtatgtggag tcccttctgt
     7021 ctgttgctta caataacact gtgtggctcc gtgcctcata aatgctttgt aattgtgagt
     7081 atttctggat tgtttcttaa acatatcata gtctgatttt tctcacatta aaataaaaca
     7141 acttgtgaaa cttagttata ctttgctgtg ttttaattaa ccttcagcag ttattaaagt
     7201 ggaatgtcag ttaaatttta agaaaaggag ataaatgcct tctgtgtttc atattgattt
     7261 attttgtgta ggagagcagc tgtttgagtt ttttggtgtg tgtgtgtttg tttttgtttt
     7321 ttgtattttt ttgataggtt tatggtttgc atgaattagt atttaaggtg attcagacta
     7381 atgcatggct tttgtataaa tccttatatt tatttctgtc ttatgaaact ttaccatgac
     7441 ctaagaagaa ttaatgggca aattaatcgg tcctccacat gactttcttt tttttttttt
     7501 tttaacataa atgggttgtt ttccaagttt caaccaattc ggctgaatct tttttattta
     7561 attgaatctt ctgtttagct tccctccgag aactctcctt tagaaagttc atgaaacttc
     7621 tcagcaaatc aatcttcctt aagtaggaag aaaggtggcc tggcctgtac gctccctttg
     7681 aattagcagc aactttccat cggtaagtat gctctggtgg agatgagatt ctcagttgta
     7741 gactagaagc cataaatgca gcgagtgctg cttgcagcac cctctggctc atttcttgcc
     7801 tttgcaagtg tatgaagtta aaagtaagaa ggcagcctta gaaggcagct atgaaaagcc
     7861 gccgttcgct ttaaaaaaat agacattcag ctccgtggaa aagccattcc cgccgtaacg
     7921 gctgtaaaga tggtggcgct gcatgctgcg tggatagtgt ctaatctgtg cttaaagtgg
     7981 ttctctcagg tttgtgtatc ttgatgcttg atgcactgtg ttttataaat agttacaaat
     8041 gttgagtaaa aaccatttct atgcactgtt ccgtgtcatt ggccttttgt gaatgtgcat
     8101 gttttaaact gcaaatttta aacattttgt cctaattgtt attaaaaaat aaaataaact
     8161 ttaccattac ataaag
//
