LOCUS       NM_009726               8195 bp    mRNA    linear   ROD 31-JUL-2024
DEFINITION  Mus musculus ATPase, copper transporting, alpha polypeptide
            (Atp7a), transcript variant 2, mRNA.
ACCESSION   NM_009726
VERSION     NM_009726.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Haberkiewicz,O., Lipinski,P., Starzynski,R.R., Jonczy,A.,
            Kurowska,P., Ogorek,M., Bednarz,A., Herman,S., Hatala,D.,
            Grzmil,P., Rajfur,Z., Baster,Z. and Lenartowicz,M.
  TITLE     Decreased Expression of the Slc31a1 Gene and Cytoplasmic
            Relocalization of Membrane CTR1 Protein in Renal Epithelial Cells:
            A Potent Protective Mechanism against Copper Nephrotoxicity in a
            Mouse Model of Menkes Disease
  JOURNAL   Int J Mol Sci 23 (19), 11441 (2022)
   PUBMED   36232742
  REMARK    GeneRIF: Decreased Expression of the Slc31a1 Gene and Cytoplasmic
            Relocalization of Membrane CTR1 Protein in Renal Epithelial Cells:
            A Potent Protective Mechanism against Copper Nephrotoxicity in a
            Mouse Model of Menkes Disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8195)
  AUTHORS   Ash,D., Sudhahar,V., Youn,S.W., Okur,M.N., Das,A., O'Bryan,J.P.,
            McMenamin,M., Hou,Y., Kaplan,J.H., Fukai,T. and Ushio-Fukai,M.
  TITLE     The P-type ATPase transporter ATP7A promotes angiogenesis by
            limiting autophagic degradation of VEGFR2
  JOURNAL   Nat Commun 12 (1), 3091 (2021)
   PUBMED   34035268
  REMARK    GeneRIF: The P-type ATPase transporter ATP7A promotes angiogenesis
            by limiting autophagic degradation of VEGFR2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8195)
  AUTHORS   Sudhahar,V., Okur,M.N., O'Bryan,J.P., Minshall,R.D., Fulton,D.,
            Ushio-Fukai,M. and Fukai,T.
  TITLE     Caveolin-1 stabilizes ATP7A, a copper transporter for extracellular
            SOD, in vascular tissue to maintain endothelial function
  JOURNAL   Am J Physiol Cell Physiol 319 (5), C933-C944 (2020)
   PUBMED   32936699
  REMARK    GeneRIF: Caveolin-1 stabilizes ATP7A, a copper transporter for
            extracellular SOD, in vascular tissue to maintain endothelial
            function.
REFERENCE   5  (bases 1 to 8195)
  AUTHORS   Gudekar,N., Shanbhag,V., Wang,Y., Ralle,M., Weisman,G.A. and
            Petris,M.J.
  TITLE     Metallothioneins regulate ATP7A trafficking and control cell
            viability during copper deficiency and excess
  JOURNAL   Sci Rep 10 (1), 7856 (2020)
   PUBMED   32398691
  REMARK    GeneRIF: Metallothioneins regulate ATP7A trafficking and control
            cell viability during copper deficiency and excess.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8195)
  AUTHORS   Starcher,B., Madaras,J.A., Fisk,D., Perry,E.F. and Hill,C.H.
  TITLE     Abnormal cellular copper metabolism in the blotchy mouse
  JOURNAL   J Nutr 108 (8), 1229-1233 (1978)
   PUBMED   27591
REFERENCE   7  (bases 1 to 8195)
  AUTHORS   Starcher,B.C., Madaras,J.A. and Tepper,A.S.
  TITLE     Lysyl oxidase deficiency in lung and fibroblasts from mice with
            hereditary emphysema
  JOURNAL   Biochem Biophys Res Commun 78 (2), 706-712 (1977)
   PUBMED   20889
REFERENCE   8  (bases 1 to 8195)
  AUTHORS   Rowe,D.W., McGoodwin,E.B., Martin,G.R. and Grahn,D.
  TITLE     Decreased lysyl oxidase activity in the aneurysm-prone, mottled
            mouse
  JOURNAL   J Biol Chem 252 (3), 939-942 (1977)
   PUBMED   14140
REFERENCE   9  (bases 1 to 8195)
  AUTHORS   Hunt,D.M.
  TITLE     Catecholamine biosynthesis and the activity of a number of
            copper-dependent enzymes in the copper deficient mottled mouse
            mutants
  JOURNAL   Comp Biochem Physiol C Comp Pharmacol 57 (1), 79-83 (1977)
   PUBMED   17511
REFERENCE   10 (bases 1 to 8195)
  AUTHORS   Hunt,D.M.
  TITLE     A study of copper treatment and tissue copper levels in the murine
            congenital copper deficiency, mottled
  JOURNAL   Life Sci 19 (12), 1913-1919 (1976)
   PUBMED   187892
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672288.5.
            
            On Dec 18, 2013 this sequence version replaced NM_009726.4.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region compared to variant 1. This
            results in a shorter protein (isoform 2) compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219380.37188.1,
                                           SRR9219383.9081.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AL672288.5         37276-37431
            157-294             AL672288.5         79822-79959
            295-784             AL672288.5         95166-95655
            785-1510            AL672288.5         96141-96866
            1511-1690           AL672288.5         98390-98569
            1691-1854           AL672288.5         102136-102299
            1855-2016           AL672288.5         104905-105066
            2017-2093           AL672288.5         106829-106905
            2094-2319           AL672288.5         107442-107667
            2320-2553           AL672288.5         108838-109071
            2554-2645           AL672288.5         110579-110670
            2646-2773           AL672288.5         111912-112039
            2774-2928           AL672288.5         112399-112553
            2929-3063           AL672288.5         112764-112898
            3064-3258           AL672288.5         115199-115393
            3259-3441           AL672288.5         117236-117418
            3442-3658           AL672288.5         119813-120029
            3659-3805           AL672288.5         125142-125288
            3806-3948           AL672288.5         128304-128446
            3949-4152           AL672288.5         130287-130490
            4153-4270           AL672288.5         131642-131759
            4271-4373           AL672288.5         133395-133497
            4374-8195           AL672288.5         134391-138212
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 47.36 cM"
     gene            1..8195
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /note="ATPase, copper transporting, alpha polypeptide"
                     /db_xref="GeneID:11977"
                     /db_xref="MGI:MGI:99400"
     exon            1..156
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            157..294
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..4650
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /EC_number="7.2.2.8"
                     /note="isoform 2 is encoded by transcript variant 2;
                     copper-transporting ATPase 1; copper pump 1; menkes
                     disease-associated protein homolog; Menkes protein;
                     ATPase, Cu++ transporting, alpha polypeptide"
                     /codon_start=1
                     /product="copper-transporting ATPase 1 isoform 2"
                     /protein_id="NP_033856.3"
                     /db_xref="CCDS:CCDS41097.1"
                     /db_xref="GeneID:11977"
                     /db_xref="MGI:MGI:99400"
                     /translation="MEPSVDANSITITVEGMTCISCVRTIEQQIGKVNGVHHIKVSLE
                     EKSATIIYDPKLQTPKTLQEAIDDMGFDALLHNANPLPVLTNTVFLTVTAPLTLPWDH
                     IQSTLLKTKGVTGVKISPQQRSAVVTIIPSVVSASQIVELVPDLSLDMGTQEKKSGAC
                     EEHSTPQAGEVMLKMKVEGMTCHSCTSTIEGKVGKLQGVQRIKVSLDNQEATIVFQPH
                     LITAEEIKKQIEAVGFPAFIKKQPKYLKLGAIDVERLKNTPVKSSEGSQQKSPSYPSD
                     STTMFTIEGMHCKSCVSNIESALSTLQYVSSIVVSLENRSAIVKYNASLVTPEMLRKA
                     IEAISPGQYRVSIASEVESTASSPSSSSLQKMPLNIVSQPLTQEAVININGMTCNSCV
                     QSIEGVISKKPGVKSIHVSLANSTGTIEFDPLLTSPETLREAIEDMGFDAALPDMKEP
                     LVVIAQPSLETPLLPSSNELENVMTSVQNKCYIQVSGMTCASCVANIERNLRREEGIY
                     SVLVALMAGKAEVRYNPAVIQPRVIAEFIRELGFGAMVMENAGEGNGILELVVRGMTC
                     ASCVHKIESTLTKHKGIFYCSVALATNKAHIKYDPEIIGPRDIIHTIGSLGFEASLVK
                     KDRSANHLDHKREIKQWRGSFLVSLFFCIPVMGLMVYMMVMDHHLATLHHNQNMSNEE
                     MINMHSAMFLERQILPGLSIMNLLSLLLCLPVQFCGGWYFYIQAYKALKHKTANMDVL
                     IVLATTIAFAYSLVILLVAMFERAKVNPITFFDTPPMLFVFIALGRWLEHIAKGKTSE
                     ALAKLISLQATEATIVTLNSENLLLSEEQVDVELVQRGDIIKVVPGGKFPVDGRVIEG
                     HSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLIRATHVGADTTLSQIVKLVEEA
                     QTSKAPIQQFADKLSGYFVPFIVLVSIVTLLVWIIIGFQNFEIVETYFPGYNRSISRT
                     ETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVK
                     VVVFDKTGTITHGTPVVNQVKVLVESNKISRNKILAIVGTAESNSEHPLGAAVTKYCK
                     KELDTETLGTCTDFQVVPGCGISCKVTNIEGLLHKSNLKIEENNIKNASLVQIDAINE
                     QSSTSSSMIIDAHLSNAVNTQQYKVLIGNREWMIRNGLVISNDVDESMIEHERRGRTA
                     VLVTIDDELCGLIAIADTVKPEAELAVHILKSMGLEVVLMTGDNSKTARSIASQVGIT
                     KVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAA
                     DVVLIRNDLLDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFLPIGLVLQPW
                     MGSAAMAASSVSVVLSSLFLKLYRKPTYDNYELHPRSHTGQRSPSEISVHVGIDDTSR
                     NSPRLGLLDRIVNYSRASINSLLSDKRSLNSVVTSEPDKHSLLVGDFREDDDTTL"
     exon            295..784
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            785..1510
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1690
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1854
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..2016
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2093
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2319
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2553
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            2554..2645
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            2646..2773
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            2774..2928
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..3063
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3258
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            3259..3441
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            3442..3658
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            3659..3805
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            3806..3948
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            3949..4152
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            4153..4270
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            4271..4373
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
     exon            4374..8195
                     /gene="Atp7a"
                     /gene_synonym="MNK"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 actttgtggg ctgggaaagc cgtcgaggga ggagctgccg ctgcctgcgc ctagactctt
       61 ctgtgtgtgc gagcgcggag gctgcgtgct ggtcaatcgc tgccgccctg cgccctgcgg
      121 agctccagag ctcgaacccc agccctggaa acccaggaat gtaaagacat caaaatggag
      181 ccaagtgtgg atgcaaattc aattactatc actgttgagg gaatgacatg tatttcctgt
      241 gtccggacca ttgaacagca gattgggaaa gtgaatggtg tccatcacat taaagtttca
      301 ctagaagaaa agagtgcaac cattatttat gaccctaaac ttcagactcc aaagaccctc
      361 caagaagcta tcgatgacat gggctttgat gctcttctcc acaatgctaa ccccctccct
      421 gtcttaacca ataccgtgtt cctgactgtt actgctccac tgactctgcc ttgggaccat
      481 atccaaagta cattgctcaa gaccaagggt gtgactggtg ttaagatttc ccctcagcaa
      541 agaagtgcag tagtcaccat aattccatct gtggtgagtg ccagtcagat tgtggagctg
      601 gtcccagacc tcagtttaga catgggaact caggagaaaa agtcaggagc ttgtgaagag
      661 cacagcacgc ctcaggcagg ggaagtcatg ctgaagatga aagtggaagg gatgacctgc
      721 cattcatgta ctagcaccat tgaaggaaaa gttggaaagc tgcaaggtgt tcagcgcatt
      781 aaagtgtccc tagacaacca agaagctact attgtttttc aacctcatct catcacagca
      841 gaggaaataa agaagcagat tgaagccgtg ggtttcccag ccttcataaa aaagcagccg
      901 aagtacctca aattgggagc cattgatgtt gagcgcctga agaacacacc ggtcaaatct
      961 tcagaaggat ctcagcaaaa gagcccatca tatcccagtg actccacaac catgttcacc
     1021 atagagggca tgcattgtaa atcgtgtgtg tccaatattg aaagtgcttt atctacactc
     1081 cagtatgtaa gcagtatagt agtttcttta gagaataggt cagccattgt aaagtacaat
     1141 gcaagcttag tcactccaga aatgctgaga aaggcaatag aggccatttc accggggcaa
     1201 tacagagtta gtattgcaag tgaagttgaa agtaccgcca gctctccctc cagctcatct
     1261 cttcagaaga tgcctttgaa catagttagc cagcctctga cccaagaagc tgtgataaac
     1321 ataaatggca tgacttgtaa ttcttgtgtg cagtctatag agggtgtaat atcaaaaaag
     1381 ccaggtgtaa aatccatcca cgtgtccctc gcaaacagca cagggactat tgaatttgat
     1441 cctctactaa cctctccaga aaccttgcga gaagcaattg aagacatggg atttgatgct
     1501 gccttgccag acatgaaaga gccactggta gtgatagctc agccctcact ggaaacacct
     1561 cttttgccct caagtaatga gctagaaaat gtgatgacgt cagttcagaa caagtgttac
     1621 atacaggtct ctgggatgac ctgtgcttct tgtgtagcaa acattgaacg caatttaaga
     1681 cgagaagaag gaatatattc tgtacttgtt gccctaatgg ctggcaaggc agaagtaaga
     1741 tataacccag ctgttatcca accccgagtg atagcagagt ttattcgaga gcttggattt
     1801 ggagctatgg tgatggaaaa cgctggggaa ggcaacggca tcttggaact tgttgtgaga
     1861 ggaatgacgt gtgcttcctg tgtccataaa attgaatcaa cactcacaaa acacaaaggg
     1921 atcttctact gctctgtggc cctggcaact aacaaagcac atattaaata tgatccagaa
     1981 attattggtc ccagagatat tatccatacc atcggaagct taggctttga agcttctttg
     2041 gtcaagaaag atcgatcagc caaccactta gatcataaac gagaaataaa acaatggaga
     2101 gggtctttcc ttgtgagcct gtttttctgt atccctgtaa tgggactgat ggtctatatg
     2161 atggttatgg atcaccacct tgcaactctt caccataatc agaacatgag taatgaagaa
     2221 atgatcaaca tgcattctgc tatgtttctg gagcgtcaga tcctgccagg actgtccatt
     2281 atgaatttgt tgtccctttt attgtgtcta cctgtacagt tttgtggcgg ctggtacttc
     2341 tacattcagg cttacaaagc actgaagcat aagacagcaa acatggatgt gctgattgtg
     2401 ctggcaacca ccatcgcatt tgcctactct ctggttattc ttctggttgc aatgtttgag
     2461 agagccaaag tgaaccccat taccttcttt gatacacctc ctatgctgtt tgtgtttatt
     2521 gcactaggac gatggctgga acatatagcg aagggcaaaa cctccgaggc tcttgcaaag
     2581 ctaatttcat tacaagcaac tgaagccact attgtaactc tgaactctga aaatctcctc
     2641 ctgagtgaag aacaagtgga tgtggaactt gtacaacgtg gagatatcat taaggttgtt
     2701 ccaggaggca aatttccagt ggatggccgt gttattgaag gacattctat ggtggacgag
     2761 tccctcatca caggggaggc aatgcctgtg gctaagaaac ctggcagcac ggtgattgca
     2821 ggttccatta accagaatgg atctctcctc atccgagcaa cacatgtggg agcagataca
     2881 accctttctc agatcgtcaa acttgtggag gaggcacaga catcaaaggc tcctatccag
     2941 cagtttgcag acaaactcag tggctacttt gttcctttca tcgtcttggt ttccattgtt
     3001 accctcttgg tgtggattat aattggattt caaaattttg aaattgtgga aacctacttt
     3061 cccggctata atagaagcat ctcccgaaca gaaaccataa tacgctttgc tttccaagcc
     3121 tctatcacgg ttctgtgtat cgcatgtccc tgttcactgg gactagccac tccaactgct
     3181 gtgatggtgg gcacaggagt aggtgctcag aatggcatac ttatcaaagg tggggagcca
     3241 ctggaaatgg ctcataaggt aaaggtagtg gtatttgata agactggaac catcacccat
     3301 ggaaccccag tagtgaatca agtgaaggtt ctcgtggaaa gtaacaagat atcacgcaat
     3361 aagatcctgg ccattgtggg gactgcagaa agtaacagtg aacatccttt aggagcagct
     3421 gtaaccaaat attgcaaaaa ggagctggac actgaaaccc tgggtacctg tacagatttc
     3481 caggttgtac caggctgtgg tattagctgt aaagtcacca atattgaagg tttgctacat
     3541 aagagtaact tgaagataga agaaaataac attaaaaatg catccctggt tcaaattgat
     3601 gcaattaatg aacagtcatc aacttcatct tctatgatta ttgatgctca tctctcaaat
     3661 gctgttaata ctcagcagta caaagtcctc attggtaacc gggaatggat gattagaaat
     3721 ggtcttgtca taagtaatga tgtagatgaa tctatgattg aacatgaaag aagaggtcgg
     3781 actgctgtct tggtgacaat cgatgatgag ctgtgtggct tgattgctat tgctgatact
     3841 gtgaaacctg aggccgagtt ggctgtacac attctgaaat ctatgggttt agaagtagtt
     3901 ctgatgactg gagacaacag taaaactgct cggtctattg cttctcaggt tggcattact
     3961 aaggtgtttg ctgaagttct accttcccac aaagttgcta aggtgaagca gctccaagag
     4021 gagggcaaac gtgtagcaat ggtaggagat ggaatcaatg actccccagc tctggcaatg
     4081 gcaaacgttg gaattgccat aggcacaggc acagatgtag ccattgaagc agctgatgtg
     4141 gttttgataa ggaatgacct tctggatgtt gtggcaagta ttgacttgtc aaggaaaaca
     4201 gtcaagagga ttcgaatcaa ttttgtcttt gctctaattt ataatctggt tggaattccc
     4261 atcgctgctg gagttttcct gcccatcggt ttggttttac aaccctggat gggatccgca
     4321 gcaatggccg cttcatctgt ctctgtagtc ctttcttccc ttttcctcaa gctttacagg
     4381 aaacctacgt atgacaatta tgagttgcat ccccggagcc acacaggaca gaggagtcct
     4441 tcagaaatca gtgttcacgt tggaatagat gacacctcaa gaaattctcc aaggctgggt
     4501 ttgctggacc ggattgtcaa ctatagcaga gcctccataa attcactgct gtctgacaag
     4561 cgctccctga acagcgttgt tactagtgaa cctgacaagc actcgcttct ggtgggcgac
     4621 ttccgggaag atgatgacac cacactgtaa aaagccttgg gggatgctgc tagttaaagt
     4681 tcacgtgcac tgacacagca cttacgttga tgtagaagga tcttatgtca atctcatgct
     4741 cttacgtgca ggattctatc tgagttgtat ttggtaatgg tgaaaatatc tttctcaggg
     4801 caggagctct gaacctggca ttatctagaa gaagttccca gcagtttttg tttcattagc
     4861 gatacagagg ggagaacagt aagtttagaa caagctctat agttagaagt tgctaatgtg
     4921 ctgctaatat ggcagatcat ttttatttga ccaaaaaaaa aaaaaaagtg cccaaagaca
     4981 ggacgattaa aaatgtgaag atttgagagc atgattttga aggtattctt gagcctatat
     5041 acttgcccca ctggggagca atggttttca aagcactgta caaagcacct agtttagaag
     5101 gaaaacagtt gcaactgtct caaaacagat gtgccttatt tattgtaggc ttgctttcac
     5161 ctcataattc atgtgtcatt gtcctttagt tcctaagagc ctgcaggatg gcttttgtca
     5221 cctcctttca agtgagccaa gtttaggtgg tttttccttg ataaaaataa ctgacaacaa
     5281 tcacttggaa tgaggcagga gaattacaag ttcaaagcca gacactaaga ccctgtctta
     5341 aaacattttc gtagctggca gctcttctga aatcttactt ttctcatcct actgtgtcgt
     5401 tcagaagacc ataagatcta cagggttctg taccctcttg tttctccact gttgtcagaa
     5461 atctcaaaat cttccttcag tttccccagc cctgtgctag ctttcagttc agctttgttc
     5521 acagtagatg cagtctctcc atcagtccca gtcgagaaac ttagaacctc tctcactgaa
     5581 aaattcttca aagattaaca ctaatatatt aacatatacg ggccatagaa aagtatgtgg
     5641 tctcattaaa ttcatctttt gggatataat cacttggctg gtatattata atttaataaa
     5701 attttagtcg cctctgtttt aaataactta tcaaaatgac tttaaaatat tctcatcgca
     5761 cagagctgct tttatcaaaa tacacaccaa actgtaatag agaaatcaga atattcctgg
     5821 tttatgatat cttgtagtta agtatgttgt tctgtcattc aaaatatttg tgtttataaa
     5881 ctcttaatct tttattttca tgtactttaa gctactagca taatgtctaa catataattt
     5941 gtgctctgaa aatgttcatt tctccttttc aagtcaatat tgagtataaa aatcatgttt
     6001 tatattactg gaatataaag tgaaatggag aatgggtttt gtccactcca ccacttttct
     6061 ctcttattcc ttcacaccca acacccacaa acgtttacat ttttcaatgc tgtctttcca
     6121 gatttggaaa agacctaact tctatcttag ctatcttaga gaggtgaaac ttttgatgaa
     6181 ggtgctatta atctagaaag gcaaacctgt tttaatgaag tatgaataag tttatatgct
     6241 taaactgtat tttaaaccaa tacttatgcc gtcttaagag tttgagtttg tatatttgtt
     6301 tcttaattct aggagatcat cataatatag ctcattggct tctagaagag agagctaaac
     6361 tatagatgtc cagtagtttt ttcttgctct gcatccatga gaacataagt cccccagtcg
     6421 tcagccagtt tctccttctg ttctgtatat gttgttgagt cctcctcctg cctccacatc
     6481 aagttatagg gcagtgagaa cagcatttca ggtctcacta ccagaaaaga acctgttgga
     6541 gagaggctgt attccttaga gaggagggtc ttttagagag gagatcaagc acgttacttt
     6601 cagttggcca ctggtttaag taatgggcca ctgctcagaa caggttttgt aagtgcatgt
     6661 atgtgtgtgt atgtgtatgt atatacacat gtatatacac acacacacat acatgcatac
     6721 agagagagac agccggagag acagagacag agagggggag agagcataca tctttcgagg
     6781 ttctttatac tttccttgcc actcttctga attcactcag caaccgttgt ccctgtcaga
     6841 aggcgccatg gacactttgc tgtccagtgc taatgcggat tcagctaggg gccacctttt
     6901 aacttaaata tccaaagatg ccttttgaat ttaagattaa aacacagcta gaatatttac
     6961 tcttgtagtt tgaaaaggat agttgttagg tgtgaaatag aaatgttgct tttcctactt
     7021 tagtatcacc attttaggaa gttacatttg atgccttatg aacaaataat tttatattat
     7081 gctttaattt tttaaaaatt ttcttcattc agatagatat agagaatgat tgaaatagca
     7141 ctatagacac gatgtttcca aaaatgtgac tgtgcatata ttttctgtac acagcttaaa
     7201 atcaaaaacg tatagtagag gatttttcca ttacgtgtag attctgtttt aaaattattt
     7261 ttcatattta ttttacttat tttactctac tttgttattt ttttgtatga ggcatctggt
     7321 taaagcttaa gctgcttctc ttcttcctct tcctcctcct cttcctcctc ctcctctccc
     7381 tcttccttct cttcttcctt ctccttttta tacagacatt cctcataatc actcctttga
     7441 gttattttgc tttctgtatt aaaaaacata cttctttacc aaatatcgat agatagatag
     7501 atagatagat agatagatag atagatagat agatagatag atagatagat agatagatag
     7561 attgattcat gccaaatctt taccaacttt tacactaact ttcttaagtc ctttcgtctg
     7621 gtatgagtag ctagctataa cacaaagcat gaattcttat ttctcaatca cattagcttc
     7681 tttaatgatg ttgataataa ttgacttttg tagccagagc caggtgtctc aaccttgtgt
     7741 ttttaaagaa atgtattatg aaattttggt tagaatccaa aacaggagcg ggcaactttg
     7801 tgcaatattt cgtttctgta gtttagtttg ttttttctca tgtgcatgta attgatattt
     7861 ctgccgaaga tgtgccttca actttataat tatagtgttg tttaaaaaaa aaatgttgtc
     7921 aagtgtcgct gtgttaagtc atccagcccc ctctatcagc agatccacac tgccctcgct
     7981 gtgctagtgt ggttgtctgg ctgttgtttc agctgtaaat ccgtgctgtg tcaggatcag
     8041 aggcagcagc ccacattcag gtaggggaca aaaatctgtt gtctctcaga tcccgctgcc
     8101 ctgctgctgt cacttagaac acgaaacaaa ggaatgtgca tgatctcttc tcctttgtag
     8161 tgtctaataa attcttgaga cggtgtctcc cctcc
//
