LOCUS       NM_029121               1005 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus ATPase, H+ transporting, lysosomal V1 subunit E2
            (Atp6v1e2), mRNA.
ACCESSION   NM_029121
VERSION     NM_029121.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Schreiber,T., Koll,N., Padberg,C., de Los Reyes,B., Quinting,T.,
            Malyshkina,A., Metzen,E., Sutter,K., Fandrey,J. and Winning,S.
  TITLE     Reduced vacuolar ATPase protects mice from Friend virus infection -
            an unintended but instructive effect in Hif-2afl mice
  JOURNAL   J Cell Sci 137 (12) (2024)
   PUBMED   38856651
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Sun-Wada,G.H., Imai-Senga,Y., Yamamoto,A., Murata,Y., Hirata,T.,
            Wada,Y. and Futai,M.
  TITLE     A proton pump ATPase with testis-specific E1-subunit isoform
            required for acrosome acidification
  JOURNAL   J Biol Chem 277 (20), 18098-18105 (2002)
   PUBMED   11872743
  REMARK    GeneRIF: novel isoform of V-ATPase E subunit located in the
            acrosomal membrane of sperm required for acrosome acidification
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154492.2 and AK015654.1.
            
            On Jul 30, 2009 this sequence version replaced NM_029121.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061059.1, BU961207.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849390
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AC154492.2         112904-112928       c
            26-1005             AK015654.1         13-992
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 56.9 cM"
     gene            1..1005
                     /gene="Atp6v1e2"
                     /gene_synonym="4930500C14Rik; Atp6e1; E1"
                     /note="ATPase, H+ transporting, lysosomal V1 subunit E2"
                     /db_xref="GeneID:74915"
                     /db_xref="MGI:MGI:1922165"
     exon            1..44
                     /gene="Atp6v1e2"
                     /gene_synonym="4930500C14Rik; Atp6e1; E1"
                     /inference="alignment:Splign:2.1.0"
     exon            45..1005
                     /gene="Atp6v1e2"
                     /gene_synonym="4930500C14Rik; Atp6e1; E1"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..826
                     /gene="Atp6v1e2"
                     /gene_synonym="4930500C14Rik; Atp6e1; E1"
                     /note="ATPase, H+ transporting, V1 subunit E-like 2;
                     ATPase, H+ transporting, lysosomal 31kDa, V1 subunit
                     E-like 2; V-ATPase subunit E 2; vacuolar proton pump
                     subunit E 2"
                     /codon_start=1
                     /product="V-type proton ATPase subunit E 2"
                     /protein_id="NP_083397.3"
                     /db_xref="CCDS:CCDS29009.1"
                     /db_xref="GeneID:74915"
                     /db_xref="MGI:MGI:1922165"
                     /translation="MALTDIDVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGR
                     LVQTQRLKIMDYFEKKEKQIEQQKKIQLSTMRNQARITVLRARDNLILELLKDAKMRL
                     SRIVSDEEIYQDLLDKLVLQALLRLLEPVMIVRCRPQDLHLVESAVLRAIPQYMRLCQ
                     KHLEVQVDQTEHLPSNAAGGVEVYSSDQKIKVSNTLESRLNLAAMQKMPEIRGILFGD
                     NTSRKFFT"
     regulatory      981..986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atp6v1e2"
                     /gene_synonym="4930500C14Rik; Atp6e1; E1"
                     /note="hexamer: AATAAA"
     polyA_site      1005
                     /gene="Atp6v1e2"
                     /gene_synonym="4930500C14Rik; Atp6e1; E1"
                     /note="major polyA site"
ORIGIN      
        1 agactcccgg cgccagacca aaggcagctc ctggcatgga agtgggaggg agcagacatt
       61 agcaacaccc tggaaactct gttctacatt gaagacacca gaaggtccac aggccctgcc
      121 tctgcccccg tttctgagag cagccatggc cctgactgac atagacgtgc agaagcagat
      181 caagcacatg atggcgttca tcgagcagga agccaacgag aaggcggagg agatagacgc
      241 caaggcggag gaagagttca acattgagaa gggccgcctg gtgcagaccc agcggctgaa
      301 gatcatggat tattttgaga agaaggagaa acagattgag cagcagaaaa agatccagct
      361 gtccaccatg agaaaccagg ctcgcatcac ggtcctgaga gcccgagaca atctcatctt
      421 agaattgctc aaggatgcca agatgaggct cagcagaatt gtgtcggatg aagaaatcta
      481 ccaggacctg ctggataagc tcgtgctcca agccttgctc cggctgctgg agcctgtgat
      541 gattgtccgc tgccggccac aggacctcca cctggtggag tctgcggtgc taagagccat
      601 ccctcagtac atgaggctct gccagaagca tttggaggta caggttgacc aaactgaaca
      661 cttgccttcc aatgctgctg gaggggtgga ggtctacagc agcgatcaga aaataaaggt
      721 ttccaacacc ctggaaagtc ggctgaatct cgcggccatg cagaagatgc cagaaatacg
      781 agggattctg tttggagaca acaccagcag aaagttcttt acatgaggct ctgggacagg
      841 gagcctgggc taaactgcag agccttcaaa gtctaagtca tttggacaaa gggaactggg
      901 gtgggggtgg ggtgtcatct tcctctgcag ccctttccta gctttgaata gctaagagtg
      961 ttatacactg ttaactttgg aataaatccc aaattatgct cagaa
//
