LOCUS       NM_001253831            4683 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus ATPase, Ca++-sequestering (Atp2c1), transcript variant
            1, mRNA.
ACCESSION   NM_001253831
VERSION     NM_001253831.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Brown,J.M. and Garcia-Garcia,M.J.
  TITLE     Secretory pathway calcium ATPase 1 (SPCA1) controls mouse neural
            tube closure by regulating cytoskeletal dynamics
  JOURNAL   Development 145 (19) (2018)
   PUBMED   30228103
  REMARK    GeneRIF: SPCA1 promotes neurulation by regulating the cytoskeletal
            dynamics
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Reinhardt,T.A., Lippolis,J.D. and Sacco,R.E.
  TITLE     The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the
            developing, lactating and involuting mammary gland parallels the
            secretory pathway Ca(2+) ATPase (SPCA1)
  JOURNAL   Biochem Biophys Res Commun 445 (2), 417-421 (2014)
   PUBMED   24530912
  REMARK    GeneRIF: The timing, magnitude of TMEM165 expression and its Golgi
            location supports a role for this Golgi Ca2+/H+ antiporter as a
            contributor to mammary Golgi calcium transport needs, in addition
            to the better-characterized roles of SPCA 1 and 2.
REFERENCE   3  (bases 1 to 4683)
  AUTHORS   Cross,B.M., Hack,A., Reinhardt,T.A. and Rao,R.
  TITLE     SPCA2 regulates Orai1 trafficking and store independent Ca2+ entry
            in a model of lactation
  JOURNAL   PLoS One 8 (6), e67348 (2013)
   PUBMED   23840669
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4683)
  AUTHORS   Shull,G.E., Miller,M.L. and Prasad,V.
  TITLE     Secretory pathway stress responses as possible mechanisms of
            disease involving Golgi Ca2+ pump dysfunction
  JOURNAL   Biofactors 37 (3), 150-158 (2011)
   PUBMED   21674634
  REMARK    Review article
REFERENCE   5  (bases 1 to 4683)
  AUTHORS   Feng,M., Grice,D.M., Faddy,H.M., Nguyen,N., Leitch,S., Wang,Y.,
            Muend,S., Kenny,P.A., Sukumar,S., Roberts-Thomson,S.J.,
            Monteith,G.R. and Rao,R.
  TITLE     Store-independent activation of Orai1 by SPCA2 in mammary tumors
  JOURNAL   Cell 143 (1), 84-98 (2010)
   PUBMED   20887894
REFERENCE   6  (bases 1 to 4683)
  AUTHORS   Sepulveda,M.R., Vanoevelen,J., Raeymaekers,L., Mata,A.M. and
            Wuytack,F.
  TITLE     Silencing the SPCA1 (secretory pathway Ca2+-ATPase isoform 1)
            impairs Ca2+ homeostasis in the Golgi and disturbs neural polarity
  JOURNAL   J Neurosci 29 (39), 12174-12182 (2009)
   PUBMED   19793975
  REMARK    GeneRIF: Impaired cellular divalent calcium ion Ca2+ homeostasis
            and/or the altered targeting of organellar proteins under
            conditions of SPCA1 knockdown highlight the importance of SPCA1
            function for normal neural differentiation.
REFERENCE   7  (bases 1 to 4683)
  AUTHORS   Sepulveda,M.R., Marcos,D., Berrocal,M., Raeymaekers,L., Mata,A.M.
            and Wuytack,F.
  TITLE     Activity and localization of the secretory pathway Ca2+-ATPase
            isoform 1 (SPCA1) in different areas of the mouse brain during
            postnatal development
  JOURNAL   Mol Cell Neurosci 38 (4), 461-473 (2008)
   PUBMED   18599310
  REMARK    GeneRIF: Has a potential role in intracellular signaling and in
            Golgi secretory processes involved in dendritic growth and in
            functional maturation of the mouse nervous system.
REFERENCE   8  (bases 1 to 4683)
  AUTHORS   Okunade,G.W., Miller,M.L., Azhar,M., Andringa,A., Sanford,L.P.,
            Doetschman,T., Prasad,V. and Shull,G.E.
  TITLE     Loss of the Atp2c1 secretory pathway Ca(2+)-ATPase (SPCA1) in mice
            causes Golgi stress, apoptosis, and midgestational death in
            homozygous embryos and squamous cell tumors in adult heterozygotes
  JOURNAL   J Biol Chem 282 (36), 26517-26527 (2007)
   PUBMED   17597066
  REMARK    GeneRIF: loss of the Golgi Ca(2+) pump causes Golgi stress,
            expansion of the Golgi, increased apoptosis, and embryonic
            lethality and demonstrates that SPCA1 haploinsufficiency causes a
            genetic predisposition to cancer.
REFERENCE   9  (bases 1 to 4683)
  AUTHORS   Mitchell,K.J., Tsuboi,T. and Rutter,G.A.
  TITLE     Role for plasma membrane-related Ca2+-ATPase-1 (ATP2C1) in
            pancreatic beta-cell Ca2+ homeostasis revealed by RNA silencing
  JOURNAL   Diabetes 53 (2), 393-400 (2004)
   PUBMED   14747290
REFERENCE   10 (bases 1 to 4683)
  AUTHORS   Shull,G.E.
  TITLE     Gene knockout studies of Ca2+-transporting ATPases
  JOURNAL   Eur J Biochem 267 (17), 5284-5290 (2000)
   PUBMED   10951186
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK133703.1, AK163049.1, AK173154.1 and BC043091.1.
            
            Transcript Variant: This variant (1) encodes the longest protein
            (isoform 1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK133703.1, SRR10223970.11809.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1233              AK133703.1         2-1234
            1234-1331           AK163049.1         1286-1383
            1332-3402           AK133703.1         1333-3403
            3403-3628           AK173154.1         3182-3407
            3629-4683           BC043091.1         3800-4854
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 56.61 cM"
     gene            1..4683
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /note="ATPase, Ca++-sequestering"
                     /db_xref="GeneID:235574"
                     /db_xref="MGI:MGI:1889008"
     exon            1..218
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..2969
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /EC_number="7.2.2.10"
                     /note="isoform 1 is encoded by transcript variant 1;
                     calcium-transporting ATPase type 2C member 1; ATPase, Ca++
                     transporting, type 2C, member 1; ATPase 2C1; ATP-dependent
                     Ca(2+) pump PMR1; Ca(2+)/Mn(2+)-ATPase 2C1; secretory
                     pathway Ca(2+)-transporting ATPase type 1"
                     /codon_start=1
                     /product="calcium-transporting ATPase type 2C member 1
                     isoform 1"
                     /protein_id="NP_001240760.1"
                     /db_xref="CCDS:CCDS57698.1"
                     /db_xref="GeneID:235574"
                     /db_xref="MGI:MGI:1889008"
                     /translation="MENLLPPSGFSYLKKYPLHSIRKYLSTLRSQRAEEQVARFQKIP
                     NVENETMIPVLTSKRASELAVSEVAGLLQADLQNGLNKSEVSHRRAFHGWNEFDISED
                     EPLWKKYISQFKNPLIMLLLASAVISILMRQFDDAVSITVAIVIVVTVAFVQEYRSEK
                     SLEELSKLVPPECHCVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLFEAVDLSVD
                     ESSLTGETAPCSKVTAPQPAANGDLASRSNIAFMGTLVRCGKAKGIVIGTGENSEFGE
                     VFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGWLLGKDILEMFTISVSL
                     AVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTLTKNE
                     MTVTHILTSDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIR
                     NNTLMGKPTEGALIALAMKMGLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPE
                     ICFMKGAYEQVIKYCTTYNSKGQTLALTQQQRDLYQQEKARMGSAGLRVLALASGPEL
                     GQLTFLGLVGIIDPPRTGVKEAVTTLIASGVSIKMITGDSQETAIAIASRLGLYSKTS
                     QSVSGEEVDTMEVQHLSQIVPKVAVFYRASPRHKMKIIKSLQKNGAVVAMTGDGVNDA
                     VALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKNFVRFQL
                     STSIAALTLISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRN
                     WKDSILTKNLILKILVSSIIIVCGTLFVFWRELRDNVITPRDTTMTFTCFVFFDMFNA
                     LSSRSQTKSVFEIGLCSNKMFCYAVLGSIMGQLLVIYFPPLQKVFQTESLSILDLLFL
                     LGLTSSVCIVSEIIKKVERSREKVQKNAGSASSSFLEV"
     exon            219..329
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            330..446
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            447..536
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            537..572
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            573..634
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            635..743
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            744..896
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            897..965
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1041
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1108
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1233
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1331
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1427
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1517
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1622
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1779
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1950
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2048
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2099
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2266
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2335
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2336..2452
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2600
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2696
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2697..2838
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2839..4666
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3382..3387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /note="hexamer: AATAAA"
     polyA_site      3405
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
     regulatory      3609..3614
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /note="hexamer: AGTAAA"
     polyA_site      3640
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /note="major polyA site"
     regulatory      4641..4646
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
                     /note="hexamer: AATAAA"
     polyA_site      4666
                     /gene="Atp2c1"
                     /gene_synonym="1700121J11Rik; ATP2C1A; BCPM;
                     D930003G21Rik; HHD; pmr1; SPCA; SPCA1"
ORIGIN      
        1 aaagtggtgg cacttcaaag gagcggctag gcagcagaga ctaagtgtct aggtcgcatt
       61 tggtcgctgt cagggagggg cagacaagga ccctcgggtt tcccctcagt atggagaact
      121 tattgcctcc gtccggattt tcttacttga aaaagtatcc tctccactct attcggaaat
      181 atttatcaac gctgagaagc caaagggccg aagagcaggt tgcacgattt caaaagatcc
      241 ctaatgttga aaatgagaca atgatccctg tactgacgtc aaagagagca agtgagttag
      301 cagtcagtga ggttgcaggc cttctccagg ctgatcttca gaatggctta aacaaatctg
      361 aagttagtca taggcgagcc ttccatggtt ggaatgaatt tgatatcagt gaagatgaac
      421 cattatggaa gaagtatatt tctcagttta aaaatcccct tatcatgctg ctcctggcgt
      481 ctgcggtcat cagcatttta atgcgccagt ttgatgatgc cgtcagtatc actgtggcaa
      541 tagtaattgt cgtcactgtg gcctttgttc aggaatatcg ttcagaaaaa tctctagaag
      601 aattgagtaa acttgtgcca ccagaatgcc attgtgtgcg tgaaggaaaa ctggagcata
      661 cacttgcccg agacttggtt ccaggtgaca cagtttgtct ctctgtgggg gacagagttc
      721 ctgcggactt acgcttattt gaggctgtgg atctttctgt ggatgagtct agcttgacag
      781 gagagacagc tccgtgctct aaagtgacag cccctcagcc agctgctaat ggagatcttg
      841 cgtcaagaag taacatcgcc ttcatgggga cactggtcag atgtggcaaa gcaaagggta
      901 ttgtcattgg aacaggagaa aattctgaat ttggagaggt ctttaagatg atgcaggcag
      961 aagaagcacc aaaaactcct ctgcagaaga gcatggacct cttaggcaag cagctgtcct
     1021 tttactcctt tggtataata ggtatcatca tgttggttgg ctggttacta ggaaaagaca
     1081 ttctggaaat gttcactatt agtgtaagct tggctgtagc tgcaattcct gaaggcctgc
     1141 ctattgtggt cacggtgact ctagcccttg gtgttatgag aatggtgaag aaaagggcta
     1201 ttgtaaagaa attgcctatt gtggaaacac tgggctgctg taatgtgatt tgttcagata
     1261 aaactggaac cctgacgaag aacgagatga ctgttactca catcctcact tccgatggcc
     1321 tgcatgctga ggtcactgga gttggctata atcagtttgg tgaagtgatc gttgatggtg
     1381 atgttgttca tggattttat aacccagctg ttagcagaat tgttgaggca ggctgtgtgt
     1441 gcaatgatgc tgtaattagg aacaacactc tgatgggaaa gccaactgaa ggagccttaa
     1501 tcgctcttgc tatgaagatg ggtcttgatg gactgcaaca agactacatc aggaaagctg
     1561 aatacccttt tagttctgag cagaagtgga tggctgttaa gtgtgtgcac cgaacacagc
     1621 aggacagacc agagatttgt tttatgaagg gtgcttatga gcaggtgatt aagtattgta
     1681 ctacatacaa cagcaaaggg cagactttgg cacttaccca gcagcagaga gatttgtatc
     1741 aacaagagaa ggcacggatg ggctcagcgg gactcagagt tcttgccttg gcgtctggtc
     1801 cggaactggg gcagctgacc ttccttggcc tggtcggaat cattgaccct cctagaactg
     1861 gtgtgaagga agctgtcaca acactcattg cctcaggagt ctccatcaaa atgatcactg
     1921 gagattctca ggagactgca attgccatcg ctagtcgtct gggattgtac tctaagactt
     1981 cacagtccgt gtctggggaa gaagtcgata caatggaggt gcagcacctt tcacagatag
     2041 tgccaaaggt tgcagtattt tacagagcaa gcccaagaca caagatgaaa attattaagt
     2101 ctctacagaa gaacggggca gttgtagcca tgacaggaga tggggtaaat gatgcagttg
     2161 ctctcaaggc tgcagacatt ggagttgcga tgggccagac tggcaccgat gtttgcaaag
     2221 aggctgcaga catgatcttg gtggatgatg atttccaaac catcatgtct gcaatagaag
     2281 agggtaaagg catttataat aacattaaaa attttgttag atttcaactg agcacgagta
     2341 tagcagcatt aactttaatc tcattggcta cgttaatgaa ctttcctaac cctctcaatg
     2401 caatgcagat tttgtggatc aatattataa tggatggacc cccagctcag agccttggag
     2461 tagagccagt ggataaagat gtcattcgaa aaccgcctcg aaactggaag gacagcattt
     2521 tgacaaaaaa cttgatactt aaaatacttg tttcatcaat aatcattgtt tgtgggactt
     2581 tgtttgtctt ctggcgagag cttcgagaca atgtgataac accccgagac acaaccatga
     2641 ctttcacttg ctttgtgttt tttgacatgt tcaatgcact gagttccaga tctcagacca
     2701 agtctgtgtt tgagattgga ctctgcagta ataagatgtt ctgctatgca gttcttggat
     2761 ccatcatggg acagttgctt gtcatttact tccctcctct ccagaaggtt tttcaaaccg
     2821 agagcctgag tatactggat ctgttgtttc ttttgggcct tacctcgtca gtgtgcattg
     2881 tgtctgagat tataaagaag gttgaaagga gccgggagaa ggtccagaag aatgctggtt
     2941 cagcatcatc gtctttcctt gaagtatgat gcatattgca ttttttttta atttgcaaac
     3001 taggaattgc agtctgagaa tcatttagaa gggcaagttc aagaagataa tgaagactta
     3061 aggacttttt taactttcca ttgactaaaa atgaccatta atgttaaaga cttgatttga
     3121 actttgttgg tagtcccaaa tgaaattatg caactttgaa taacacattc cttgatttga
     3181 attggctttt gcaattgagt gaacttcaac atatgggcaa gtactgaatt aaaaaataca
     3241 aaaggcaaaa cctgaaccac cttctgcact taaagaattc taatgtacaa atacactgtc
     3301 tgtcttagat ggatataaat atttttttta tttttaaata ttgtactatt tatggttgtg
     3361 gggctttctt actaacacac aaataaactc aatcatttaa aggcattcta tttggtttag
     3421 aaattgccag gggtgccgta tttcagctac cgtgcttcct ttttcctgaa atgtaagcaa
     3481 ctgatacctt gtgctaccat ttttatattt caagttgttt tttttttttt tttgcacagg
     3541 atgtgaccac tgtctaatca ctgttctttt ctttcttttt gtgactgaaa agcatatact
     3601 gcaattgaag taaatgtttg ctcttcttag taagtgtaaa tggttgtttt ttggtttttt
     3661 tgtagttttg ttttgcatag aggaacgagt ctttgatcta gcagaattat tagttcaggg
     3721 ctgttattgg cattacagtt agagtaattg tagttaacac acagccatag agtcaaaaac
     3781 aaggctgggt tggaatttaa aacattatat taacatgtcc atgtttagtt taatactgct
     3841 ttgtcggtat cattttgtca tttcttcttt ataaatcaaa cacttcagct gaataattga
     3901 cctttattac tgtttcatat ctgtattttt aagtctattt cttagagagt atagactagt
     3961 atacttacaa aatagaaaag tcccaaactc agagagatag catatgaacc agggtgtaca
     4021 ttggaagatc ctgtgctggc cgcctcctcc gaatcctcta ggcagctgct ttgacctaga
     4081 ttgtagcaca accctgagtc tgtcccttct accatttgtt gtactgtctt ctacttcatt
     4141 cagtatgctt gggaaataga tgagtttgtt ttgtgcaaca ccatctgttt ttataaaagc
     4201 tcgataaaat aaaaggtaag tgttttgtat tttataacct gtcaggatta ataactgttc
     4261 ctaagcttgt tcaatattac tggtgactgt caacactggt gtgctgtcat tgataggaga
     4321 gttctgttgt ttgtgtctgt actactgaag cacgagcata aggggccagg tgtctgcact
     4381 ttgttcttta ttctttctgg ctcaactgtc aagtgtacat tgttctgtct gattaggtgg
     4441 aacgtgaaat cattgtctga ccaactctta gaggtttgat gtctcatagc aaagttttat
     4501 aatggttttt tacattctgt tccagacttg aaaactacat attaagtgca tattcagtct
     4561 tgtttattat acagtagagg ttgagaatat gactcatgaa atgaatgtgc ctatcttttc
     4621 acttggtatt gaattataaa aataaaagga gtttttcata agttcaaaaa aaaaaaaaaa
     4681 aaa
//
