LOCUS       NM_213616               8101 bp    mRNA    linear   ROD 28-FEB-2023
DEFINITION  Mus musculus ATPase, Ca++ transporting, plasma membrane 4 (Atp2b4),
            transcript variant b, mRNA.
ACCESSION   NM_213616 XM_355232
VERSION     NM_213616.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Beckmann D, Langnaese K, Gottfried A, Hradsky J, Tedford K, Tiwari
            N, Thomas U, Fischer KD and Korthals M.
  TITLE     Ca2+ Homeostasis by Plasma Membrane Ca2+ ATPase (PMCA) 1 Is
            Essential for the Development of DP Thymocytes
  JOURNAL   Int J Mol Sci 24 (2), 1442 (2023)
   PUBMED   36674959
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Korthals M, Tech L, Langnaese K, Gottfried A, Hradsky J, Thomas U,
            Zenclussen AC, Brunner-Weinzierl MC, Tedford K and Fischer KD.
  TITLE     Plasma membrane Ca2+ ATPase 1 (PMCA1) but not PMCA4 is critical for
            B-cell development and Ca2+ homeostasis in mice
  JOURNAL   Eur J Immunol 51 (3), 594-602 (2021)
   PUBMED   33098669
  REMARK    GeneRIF: Plasma membrane Ca(2+) ATPase 1 (PMCA1) but not PMCA4 is
            critical for B-cell development and Ca(2+) homeostasis in mice.
REFERENCE   3  (bases 1 to 8101)
  AUTHORS   Sun R, Liang H, Guo H, Wang Z and Deng Q.
  TITLE     PMCA4 gene expression is regulated by the androgen receptor in the
            mouse testis during spermatogenesis
  JOURNAL   Mol Med Rep 23 (2) (2021)
   PUBMED   33355366
  REMARK    GeneRIF: PMCA4 gene expression is regulated by the androgen
            receptor in the mouse testis during spermatogenesis.
REFERENCE   4  (bases 1 to 8101)
  AUTHORS   Stafford N, Zi M, Baudoin F, Mohamed TMA, Prehar S, De Giorgio D,
            Cartwright EJ, Latini R, Neyses L and Oceandy D.
  TITLE     PMCA4 inhibition does not affect cardiac remodelling following
            myocardial infarction, but may reduce susceptibility to arrhythmia
  JOURNAL   Sci Rep 11 (1), 1518 (2021)
   PUBMED   33452399
  REMARK    GeneRIF: PMCA4 inhibition does not affect cardiac remodelling
            following myocardial infarction, but may reduce susceptibility to
            arrhythmia.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8101)
  AUTHORS   Rattner A, Terrillion CE, Jou C, Kleven T, Hu SF, Williams J, Hou
            Z, Aggarwal M, Mori S, Shin G, Goff LA, Witter MP, Pletnikov M,
            Fenton AA and Nathans J.
  TITLE     Developmental, cellular, and behavioral phenotypes in a mouse model
            of congenital hypoplasia of the dentate gyrus
  JOURNAL   Elife 9, e62766 (2020)
   PUBMED   33084572
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8101)
  AUTHORS   Schuh K, Cartwright EJ, Jankevics E, Bundschu K, Liebermann J,
            Williams JC, Armesilla AL, Emerson M, Oceandy D, Knobeloch KP and
            Neyses L.
  TITLE     Plasma membrane Ca2+ ATPase 4 is required for sperm motility and
            male fertility
  JOURNAL   J Biol Chem 279 (27), 28220-28226 (2004)
   PUBMED   15078889
  REMARK    GeneRIF: PMCA4 has a pivotal role in the regulation of sperm
            function and intracellular Ca(2+) levels
REFERENCE   7  (bases 1 to 8101)
  AUTHORS   Krizaj D, Demarco SJ, Johnson J, Strehler EE and Copenhagen DR.
  TITLE     Cell-specific expression of plasma membrane calcium ATPase isoforms
            in retinal neurons
  JOURNAL   J Comp Neurol 451 (1), 1-21 (2002)
   PUBMED   12209837
  REMARK    GeneRIF: PMCA4 was found to be selectively expressed in both
            synaptic layers of the retina
REFERENCE   8  (bases 1 to 8101)
  AUTHORS   Shull GE.
  TITLE     Gene knockout studies of Ca2+-transporting ATPases
  JOURNAL   Eur J Biochem 267 (17), 5284-5290 (2000)
   PUBMED   10951186
  REMARK    Review article
REFERENCE   9  (bases 1 to 8101)
  AUTHORS   Garcia ML and Strehler EE.
  TITLE     Plasma membrane calcium ATPases as critical regulators of calcium
            homeostasis during neuronal cell function
  JOURNAL   Front Biosci 4, D869-D882 (1999)
   PUBMED   10577388
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 8101)
  AUTHORS   Zacharias DA and Kappen C.
  TITLE     Developmental expression of the four plasma membrane calcium ATPase
            (Pmca) genes in the mouse
  JOURNAL   Biochim Biophys Acta 1428 (2-3), 397-405 (1999)
   PUBMED   10434059
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK143672.1, BC109172.1, EU493095.2, AC124338.11 and CJ240063.1.
            
            [WARNING] On May 10, 2023 this sequence was replaced by
            NM_213616.5.
            
            On Jan 7, 2012 this sequence version replaced NM_213616.3.
            
            Transcript Variant: This variant (b, also known as x/b or PMCA4b)
            lacks an alternate exon which results in an alternate 3' coding
            region, compared to variant a. The encoded isoform (b) has a
            distinct and longer C-terminus, compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU493095.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-466               AK143672.1         122-587
            467-1963            BC109172.1         1-1497
            1964-4156           EU493095.2         1668-3860
            4157-7484           AC124338.11        12424-15751
            7485-8101           CJ240063.1         81-697
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.95 cM"
     gene            1..8101
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /note="ATPase, Ca++ transporting, plasma membrane 4"
                     /db_xref="GeneID:381290"
                     /db_xref="MGI:MGI:88111"
     exon            1..659
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     CDS             467..4084
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /EC_number="7.2.2.10"
                     /EC_number="7.2.2.10"
                     /note="isoform b is encoded by transcript variant b;
                     plasma membrane calcium-transporting ATPase 4"
                     /codon_start=1
                     /product="plasma membrane calcium-transporting ATPase 4
                     isoform b"
                     /protein_id="NP_998781.1"
                     /db_xref="CCDS:CCDS15298.1"
                     /db_xref="GeneID:381290"
                     /db_xref="MGI:MGI:88111"
                     /translation="MTNPPGQSVSANTVAESHEGEFGCTLMDLRKLMELRGADAVAQI
                     SAHYGGVQEICTRLKTSPIEGLSGNPADLEKRRLVFGKNVIPPKRPKTFLELVWEALQ
                     DVTLIILEIAAIISLVLSFYRPPGGDNEICGHIASSPEEEEEGETGWIEGAAILASVI
                     IVVLVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGDIAQIKYGD
                     LLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGV
                     NSQTGIIFTLLGASEEEDDDDKKKKGKKQGAPENRNKAKTQDGVALEIQPLNSQEGLD
                     SEDKEKKIARIPKKEKSVLQGKLTRLAVQIGKAGLIMSVLTVVILILYFVVDNFVIQR
                     REWLPECTPVYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRH
                     LDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQIPQPDVFPPKVLELIVNG
                     ISINCAYTSKIQPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRNEVPEEKLFKV
                     YTFNSVRKSMSTVIRKPEGGFRMFSKGASEIMLRRCDRILNKEGEIKSFRSKDRDNMV
                     RNVIEPMASEGLRTICLAYRDFDGTEPSWDIEGEILTSLICIAVVGIEDPVRPEVPDA
                     IAKCKRAGITVRMVTGDNVNTARAIATKCGILTPKDDFLCLEGKEFNSLIRNEKGEVE
                     QEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTAGEQRQVVAVTGDGTNDGPALKKAD
                     VGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAV
                     IVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLRRRPYGRNKPLIS
                     RTMMKNILGHAVYQLLIVFLLVFAGDTLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQ
                     LFNEINARKIHGEKNVFAGVYRNIIFCTVVLGTFFCQIMIVELGGKPFSCTSLTMEQW
                     MWCLFIGIGELLWGQVISAIPTKSLKFLKEAGHGSDKEDISRDTEGMDEIDLAEMELR
                     RGQILWVRGLNRIQTQIRVVKLFHNNHEVAHKPKNRSSIHTFMTQPEYPADDELSQSF
                     LDIQEGNPELVSKAGTSVLLLDGEAASHDNINNNAVDCHQVQIVASHSDSPLPSLETP
                     V"
     exon            660..857
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            858..1115
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1241
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1370
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1406
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1568
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1783
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..2026
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2268
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2500
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2680
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            2681..2768
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2875
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            2876..3067
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            3068..3281
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            3282..3493
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            3494..3601
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            3602..3778
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
     exon            3779..8101
                     /gene="Atp2b4"
                     /gene_synonym="Pmca4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcctagccct catcgttatc cagggagccc tcctcttttt cctgacgcca gcctaccatc
       61 gtaattgctg attgttgcta aggttgcttc catggtaaca tgcttatcct gtttacactt
      121 ctatatggac aagaagtcta aactaggaac ctacctactc tggacaacca ttcccatctc
      181 cacccagagg acaccaatca tcgtgacacc acgtccatct tttactaagt tcccccaacc
      241 tcttcctctt ctcactagta gacttaatac agaagcaagg atctaggccg ggtgttgcta
      301 ctctgaaact tattgcacga gatattgtca atttatcccc taactgacct cccagagaag
      361 aaagaagtgg gaagaaacgg cgaatagaag gcaagagaca ctgatcagtt agaggggaaa
      421 ctctacatct tctctggttg agagtctggt aactgaagaa ggcaaaatga cgaatccacc
      481 aggacaaagc gtgtcagcca acacagtggc tgagagccat gaaggggagt ttggctgcac
      541 cttaatggac ctgcgaaagc tcatggagct tcgtggagct gatgcagtgg cccagatcag
      601 tgcccactac ggaggggtac aggaaatctg cactagactg aaaacctccc ctatagaagg
      661 tctatctggg aaccctgcag atttggagaa gcgtagactt gtttttggaa agaacgtgat
      721 acctccaaaa aggcccaaga ctttcttaga attagtgtgg gaagccctgc aggatgtcac
      781 gctcatcatc ctagagatcg cagccatcat ctccctggtc ctgtccttct accgacctcc
      841 gggtggagat aatgaaatct gtggtcacat tgcaagtagc ccagaagaag aggaggaagg
      901 agaaactggc tggattgagg gggcggccat tctcgcctcg gtgatcattg tggtactcgt
      961 gacagccttc aatgactgga gcaaagagaa gcagttccgg gggctgcaga gtcgcatcga
     1021 actggagcaa aaattctcca ttatccggaa tggtcagctt atccaactcc cagtggctga
     1081 gattgtggtg ggagatattg cccagatcaa atacggtgac ctgctgcctg cagatggaat
     1141 tctaatccag ggaaatgatc tgaagattga cgagagctct ctgacaggag aatcagatca
     1201 tgtcaagaag actctggaca aagaccccat gttgctctcg gggactcacg tgatggaagg
     1261 ttctggacgg atggtagtga ctgctgtggg ggtcaactcc cagactggaa tcatcttcac
     1321 cctcttagga gctagtgagg aagaagatga tgatgacaag aagaagaaag gtaaaaaaca
     1381 aggagcccct gaaaatcgca acaaagcaaa gacccaggat ggagtggcct tggaaatcca
     1441 gccactcaac agccaagagg ggctcgacag tgaggacaag gagaagaaaa tagccaggat
     1501 acccaagaaa gagaagtcgg tgctgcaagg caaactcaca cgcctggctg tgcagatcgg
     1561 gaaggcgggt ctgatcatgt ctgtcctcac agttgtcatc ttgattctgt actttgtggt
     1621 tgacaatttc gtgatccagc gccgagaatg gcttcctgag tgcactcctg tctatatcca
     1681 gtattttgtc aagttcttca tcatcggagt cactgtattg gtagtggctg tgccagaggg
     1741 gctgccactg gctgtcacca tctcactagc ctactctgta aagaaaatga tgaaggacaa
     1801 caacttggta cggcacttgg atgcttgtga gacaatgggc aatgccacag ccatttgctc
     1861 cgataagaca ggcacactga ccatgaaccg catgaccgtg gtgcaagctt acatcggggg
     1921 cactcactac cgccagatcc cacagcccga cgtcttccca cccaaggttc tggagctcat
     1981 cgtcaatggc atctctatca actgtgctta cacgtctaag attcagcctc cagaaaagga
     2041 gggaggccta ccccgacaag taggcaacaa gacagagtgt gggctgctgg gctttgtcac
     2101 agacctgaag caggactacc aagcagttcg caatgaggtg cctgaggaga agctctttaa
     2161 ggtgtacacc ttcaactcag tgcgcaagtc catgagcact gtcatcagga agccggaagg
     2221 gggcttccgc atgttcagca agggcgcctc tgagataatg ctgcgcaggt gtgatcgaat
     2281 cctgaacaag gaaggagaaa tcaaatcgtt cagaagtaag gaccgagata atatggtacg
     2341 caatgtcatt gagcccatgg ccagtgaagg gctccggact atctgcttag cttaccgaga
     2401 ttttgatggc acagagccct cgtgggacat tgagggtgaa atcctcactt cgctgatctg
     2461 cattgcagtg gtgggcattg aggatcctgt gcgcccggag gtaccagatg caattgccaa
     2521 atgcaaacgg gctggcatta ctgtgaggat ggtgacaggt gataatgtca acacagcccg
     2581 ggccattgcc accaaatgtg gcattctgac tcctaaagat gacttcttgt gcctggaagg
     2641 caaagaattc aacagtctta tccgaaatga gaagggtgag gttgaacaag aaaagctgga
     2701 caagatctgg cccaagcttc gggtactggc acgatcttct cccacagaca agcacacatt
     2761 ggtgaaaggt atcattgaca gcactgctgg ggaacagcgg caggtagtgg ctgtcaccgg
     2821 tgatggaaca aatgatggac ctgctctgaa gaaagcagat gtcgggtttg ctatgggcat
     2881 cgcaggcaca gatgtggcta aggaggcgtc agacatcatc ctgaccgatg acaacttcac
     2941 cagcattgtg aaggcagtga tgtggggacg gaatgtgtat gacagcattt ccaagttcct
     3001 acagttccag ctcactgtca atgtggtggc cgtgattgtg gctttcactg gagcctgcat
     3061 cactcaggat tccccactga aagcggtgca gatgttgtgg gttaacctga ttatggacac
     3121 gtttgcttcc ctggccctgg ccacagagcc ccccaccgag tctctgttga ggcggcgccc
     3181 ctatgggcga aacaagcctc tgatctcccg cactatgatg aagaacatct tgggccatgc
     3241 tgtgtatcag ctcctaatcg tcttcctcct cgtctttgct ggcgacacac tgttcgacat
     3301 tgacagtggg aggaaggcac ctctcaattc gccacccagc cagcactaca ccattgtttt
     3361 caacacgttt gtgctgatgc aactcttcaa cgaaatcaac gctcggaaga tccacggtga
     3421 gaagaacgtg tttgcaggcg tctaccgcaa catcattttc tgcactgtag ttttgggcac
     3481 attcttctgc cagatcatga ttgttgaact gggagggaag cccttcagct gcacaagcct
     3541 gaccatggag cagtggatgt ggtgtctctt tatagggatt ggagaacttt tgtggggtca
     3601 ggtcatctct gcaataccta ccaagtctct gaagttcctg aaggaggctg gccacggcag
     3661 tgacaaagag gacatcagca gggatactga gggaatggac gagattgacc ttgccgagat
     3721 ggagcttcgc cgaggccaga tcctctgggt ccgtggcctg aaccggattc agactcagat
     3781 cagagtcgtc aaactgttcc ataataacca tgaagttgct cataaaccca agaatcgtag
     3841 ctccatccac accttcatga cccaacctga ataccccgca gatgatgaat tgtcacaatc
     3901 cttcctggat atccaagagg gcaatcctga attggtttct aaggcgggga cgagtgtgct
     3961 cctgttggat ggtgaagctg cttcgcacga caacataaat aacaacgccg tggactgcca
     4021 ccaagtgcaa atcgttgcct cccattctga cagccctctg ccaagcctgg agacaccggt
     4081 ctgaacttcc attctctgag tcccgcccct gctctccctg ttcctcttct tctgtcccat
     4141 ctgtatgtga cggtgggact tgtcattgtt gtgtcagctg ctcctaagta tgtgagaacc
     4201 cccacccgac catgaagaag caggagcaca gacctacctg gattcagtca aaccctgggc
     4261 aggtaacggt agatccactc ctcaggtgta ctcgctgtca ttttaaagag atggtggcaa
     4321 acctcttggc atcacccaac ccaaaatctc cacagcactc ccacatgcgc caccacctcc
     4381 tcttgactcc aaaattttat ccaatgagtt tctgccattt acatttacaa gctaagctgt
     4441 gggctcggtg caagacaaat accctgggtt tgtgtttcag tagagtggtt tttcttaaaa
     4501 catttttttt ttcctatttt gaaaatgtgc actctaaggc ctccagctct ttctgccctt
     4561 cattctgatg tcccctcaag gttcttatat ggctgatatc tctctctctc tctctctctc
     4621 ggtagttata agaatggaaa ggacatggac aagttgagcc aactttgacc ctttctggcc
     4681 ccagtagaac ctttcattct gttctagagg caggcagatt tctttgctaa aaatggtctt
     4741 tttggagcaa atgagctggt gaaatatttc cctattctta ccaggtcagc acccaccttc
     4801 tgaatgtctg tcttcttggg tatatagggg ttttgaagtg ccacggcaat cagccctaac
     4861 catggggctt gtgcatgtgg aatctatagt ttttaccgta ggcctgagct tccttcttgc
     4921 ccagggaggt agcagaagag tgaatgggtc atgcccctgc ctcctagatg gttgaggagc
     4981 ctcagaagga ccaggagtca ctaaagcaga cttctttcaa gggtttttgc caaggctctc
     5041 ccagggcagg agcttctggg agagggtagg ggggatgagt gttaagaagt cttgggaagg
     5101 gctaaagaac cagtccttca ggatctgaga aacctcccta cactttgccc cacatcattt
     5161 tctgtctcta aggaggcccc taacttctgc aaacattcta atgttgaaaa tgcatagcca
     5221 caagaggagg ctccctgaca gcatgctggt tcaaggagca aaaggctctc cctgggaagc
     5281 cacctcctcc caccccccac caccaccccc cacccccgtt ttcatgcacc ctcctccagc
     5341 gtctctactg ctgcccaggg aagtctgtgc ctttctgccc caacactgcc cagagcaccc
     5401 tctgcccgcc cgcctctcag gagctgggaa cttcaccaaa agggactttg ccttactggg
     5461 acaaggagga ggcccagtcc caggtgctcc atgtgtatct ccgctgggct gatttaagca
     5521 tttttgtgca ggtggatgga atgtatgtcc ccaagtccca cctcctgtta ggtcagtgaa
     5581 gccttcctcc ctctttccag ggtaatggga ataagagggt gcctgaggct catgagaaca
     5641 gccaggtttc cgcttgggaa aggaaagtga aatgagcttg cccactcttc ttctagcgtt
     5701 cttcctccca ccccaccttt cccccacgct cccaggggag ggtgagtgta gggacaaaca
     5761 ccatcccaca cccattccag cataatccat cattagagga agtagggaga aggctttgat
     5821 tgggattttg taaagcttac acatttcggc aatctttcct ctttgggggg aaaggtatct
     5881 cactcaaggt gttgttgttc aactatatat atttaagtac attagaagat tggattctgt
     5941 caactctttt attacctcag attattttta aaaaataagc caataacaag ttttgaaggc
     6001 cattttacca tccttgctcc caaacaattc tgtggtgcct gacaggttaa ctcctgaggg
     6061 cttgtgtcta ctttttcaaa gtcaacaggt ttttctgtgt atgcgtgttc tgtagtttcc
     6121 atagtaagag agaaaatata gaaatattat gcaaaaaaat atagttttct ttaggtcagg
     6181 aactgacatt tattagtcag ccatatatct atatctatat ctctctatat atatatattg
     6241 tttaaagaat ttcaaataaa gtgccaccat gtcacaccgt ggagaggagc atcaccagct
     6301 gtttggcatg acaggtgact cagtatgttt gtaattggtt aaaaacaaca accaatatac
     6361 tatacttact ttctccaaac agccatcttt atatttaggg aaaaaatttg ttgggttcta
     6421 gactttttta atataaattt tgttgataca gaattgaata agttcaagtg tttctgtgca
     6481 tatgttttta tatgtttttt ttttctattc agtttcagta atccaggtgg cagtgtgtaa
     6541 atatgatatc agttatgaca ctttccccta aatggtgctt cggatttgaa agaggtcaga
     6601 tggggagaga gaatatatcc tagagccttc ttttgataaa cctggataga taggtaagaa
     6661 cctgctggta gtgaaggaag cacccacaaa gaggctcctt agctgtgggg ccacggcagg
     6721 tgctgccgtg gaggagagcc aggcttgttg gtactcagga gtgaggatca gttgggtttc
     6781 tgtttcttaa gaaattagtg ggcagagtag tatcaatgat ggatggtctc tgctagtgag
     6841 caagggtact ccaatgtggg gtctacagtt ctttatgctt ccttcccttt ctcctccccc
     6901 cggagagacc ccactagtga ggttctgtgt atgactctga gttgtaacaa cctgctgata
     6961 cagcagagag gagccagagc tccaaactgg aaaagatcaa tgcaacaaca atggctactt
     7021 gtagctccgg ctgacagtct caggactagg ccttctcaat agttcttcca tgtcctttcc
     7081 agtacttttc ccatcccaaa agctgtagga gagaccagct agaaaccctc cggacccttg
     7141 gaagttcctc ggggttggat ttcttttttg ccttcaagct ttaggggaga gttgcaaaga
     7201 tcaatcagag ggattacctc actgccaaaa acacaaggga acttgagtgt gcccgaatcc
     7261 tcgcccagac cccgctcttt ctccccactc ccctcgcctt acagttgaag ctaccagtgg
     7321 caccattcca gtctgcacat ccaatcactt tctgcatcta gggtccaccc catggcactt
     7381 aggggtgggc acttgcaatg cctgctgttc taaagccatt ccggcctcct cctcagaata
     7441 gaccagatac tctcggtccc ttccgtttta gctcagagcc agaactttta ccttcccagt
     7501 ctgcagttcg gcctgctgcc tcctttcttc ttggttggga gaggtgaagg aaggtgagat
     7561 tccctgcaac ggaatcgacc tttggcccgt attttctccc tgtatgtata tatgccatat
     7621 gtaaatatgc catatatgtg ccaacaaatt gttctacgcc atcttttcta atcagcatgc
     7681 agataggaat tttgagtttc ttctcagtaa ctagtataat gagcactggt atttttgtac
     7741 aaaacaaaaa gaaaaaaaaa ttgacttttg tggtctgcat gacagacatg ttatcaaagc
     7801 aatgtatacc ctagcgtgtg tggccatagt ttgcagttca gctgagcagt gcacccaatc
     7861 tgtatttgca tttgatatta tttaagctct atgtataatg ttttgcatgt atttatatgg
     7921 ttcttgggag gggggatgct agaaactgac aaatctatac tgcaaatgtg ttatacaact
     7981 gacacgggaa gaaaaggagt tttaaagggg gtgacttttt ggaaccaata aagctttttg
     8041 ctgatgaaca gaaaccaatg ccatgcactg agaataaaaa cccatgccca cttgtaatgt
     8101 g
//
