LOCUS       NM_138652               3954 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ATPase, H+/K+ transporting, nongastric, alpha
            polypeptide (Atp12a), mRNA.
ACCESSION   NM_138652
VERSION     NM_138652.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Ayasse,N., Berg,P., Andersen,J.F., Svendsen,S.L., Sorensen,M.V.,
            Fedosova,N.U., Lynch,I.J., Wingo,C.S. and Leipziger,J.
  TITLE     Benzamil-mediated urine alkalization is caused by the inhibition of
            H+-K+-ATPases
  JOURNAL   Am J Physiol Renal Physiol 320 (4), F596-F607 (2021)
   PUBMED   33554781
REFERENCE   3  (bases 1 to 3954)
  AUTHORS   Walter,C., Rafael,C., Genna,A., Baron,S. and Crambert,G.
  TITLE     Increased colonic K+ excretion through inhibition of the H,K-ATPase
            type 2 helps reduce plasma K+ level in a murine model of nephronic
            reduction
  JOURNAL   Sci Rep 11 (1), 1833 (2021)
   PUBMED   33469051
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3954)
  AUTHORS   Walter,C., Rafael,C., Lasaad,S., Baron,S., Salhi,A. and Crambert,G.
  TITLE     H,K-ATPase type 2 regulates gestational extracellular compartment
            expansion and blood pressure in mice
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 318 (2), R320-R328 (2020)
   PUBMED   31913688
  REMARK    GeneRIF: H,K-ATPase type 2 regulates renal salt and potassium
            metabolism during gestation, controlling plasma volume expansion
            and blood pressure.
REFERENCE   5  (bases 1 to 3954)
  AUTHORS   Cheval,L., Bakouh,N., Walter,C., Tembely,D., Morla,L., Escher,G.,
            Vogt,B., Crambert,G., Planelles,G. and Doucet,A.
  TITLE     ANP-stimulated Na+ secretion in the collecting duct prevents Na+
            retention in the renal adaptation to acid load
  JOURNAL   Am J Physiol Renal Physiol 317 (2), F435-F443 (2019)
   PUBMED   31188029
REFERENCE   6  (bases 1 to 3954)
  AUTHORS   Zhang,W., Kuncewicz,T., Higham,S.C. and Kone,B.C.
  TITLE     Structure, promoter analysis, and chromosomal localization of the
            murine H(+)/K(+)-ATPase alpha 2 subunit gene
  JOURNAL   J Am Soc Nephrol 12 (12), 2554-2564 (2001)
   PUBMED   11729223
REFERENCE   7  (bases 1 to 3954)
  AUTHORS   Spicer,Z., Clarke,L.L., Gawenis,L.R. and Shull,G.E.
  TITLE     Colonic H(+)-K(+)-ATPase in K(+) conservation and electrogenic
            Na(+) absorption during Na(+) restriction
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 281 (6), G1369-G1377 (2001)
   PUBMED   11705741
REFERENCE   8  (bases 1 to 3954)
  AUTHORS   Pestov,N.B., Romanova,L.G., Korneenko,T.V., Egorov,M.V.,
            Kostina,M.B., Sverdlov,V.E., Askari,A., Shakhparonov,M.I. and
            Modyanov,N.N.
  TITLE     Ouabain-sensitive H,K-ATPase: tissue-specific expression of the
            mammalian genes encoding the catalytic alpha subunit
  JOURNAL   FEBS Lett 440 (3), 320-324 (1998)
   PUBMED   9872395
REFERENCE   9  (bases 1 to 3954)
  AUTHORS   Meneton,P., Schultheis,P.J., Greeb,J., Nieman,M.L., Liu,L.H.,
            Clarke,L.L., Duffy,J.J., Doetschman,T., Lorenz,J.N. and Shull,G.E.
  TITLE     Increased sensitivity to K+ deprivation in colonic
            H,K-ATPase-deficient mice
  JOURNAL   J Clin Invest 101 (3), 536-542 (1998)
   PUBMED   9449685
REFERENCE   10 (bases 1 to 3954)
  AUTHORS   Ono,S., Guntupalli,J. and DuBose,T.D. Jr.
  TITLE     Role of H(+)-K(+)-ATPase in pHi regulation in inner medullary
            collecting duct cells in culture
  JOURNAL   Am J Physiol 270 (5 Pt 2), F852-F861 (1996)
   PUBMED   8928848
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC109011.1 and BY663985.1.
            
            On Sep 13, 2007 this sequence version replaced NM_138652.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC109011.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849389, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3649              BC109011.1         1-3649
            3650-3954           BY663985.1         39-343
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 28.19 cM"
     gene            1..3954
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /note="ATPase, H+/K+ transporting, nongastric, alpha
                     polypeptide"
                     /db_xref="GeneID:192113"
                     /db_xref="MGI:MGI:1926943"
     exon            1..222
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..3321
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /EC_number="7.2.2.19"
                     /EC_number="7.2.2.13"
                     /note="proton pump; non-gastric H(+)/K(+) ATPase subunit
                     alpha; ATPase H+K+-transporting, alpha 2; HK alpha 2;
                     sodium pump; non-gastric Na(+)/K(+) ATPase subunit alpha"
                     /codon_start=1
                     /product="potassium-transporting ATPase alpha chain 2"
                     /protein_id="NP_619593.2"
                     /db_xref="CCDS:CCDS27148.1"
                     /db_xref="GeneID:192113"
                     /db_xref="MGI:MGI:1926943"
                     /translation="MRRKTEIYSVELNGTKDVELADQKDDKKFKGGKNKDSEPNKSQE
                     EELKKELDLDDHRLSNTDLEQKYGTNIIQGLSSIRAAELLARDGPNALTPPKQTPEII
                     KFLKQMVGGFSILLWIGAALCWIAYVIQYVSSTASLDNVYLGAILVLVVILTGIFAYY
                     QEAKSTNIMASFSKMIPQQALVIRDAEKKIIPAEQLVVGDVVEIKGGDQIPADIRLVF
                     SQGCKVDNSSLTGESEPQARSTEFTHENPLETKNIGFYSTTCLEGTATGIVINTGDRT
                     IIGRIASLASGVGSEKTPIAIEIEHFVHIVAAVAVSVGVIFFITAVCMKYYVLDAIIF
                     LISIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTL
                     TQNRMTVAHLWFDNQIFVADTSENQTKQAFDQSSGTWASLSKIITLCNRAEFRPGQES
                     VPIMKRVVVGDASETALLKFSEVILGDVMDIRKRNHKVAEIPFNSTNKFQLSIHETED
                     PNDKRFLMVMKGAPERILEKCSTIMINGQEQPLDKSSADAFHTAYMELGGLGERVLGF
                     CHLYLPADKFPQSYTFDVDSINFPTSNLCFVGLLSMIDPPRSTVPDAVSKCRSAGIKV
                     IMVTGDHPITAKAIAKSVGIISANNETVEDIAKRRNIAVEQVNKREAKAAVVTGMELK
                     DMTPEQLDELLINYQEIVFARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKAD
                     IGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYTLTKNIAEL
                     CPFLIYIVAGLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKKKDRLV
                     NKQLAIYSYLHIGLMQALGGFLVYFTVYAQQGFWPTSLINLRVSWETDDINDLEDSYG
                     QEWTRYQRKYLEWTGSTAFFVAIMVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGIIS
                     QIIVALVLSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEMRKLFIRLYPGSWWD
                     KNMYY"
     exon            223..372
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            373..432
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            433..633
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            634..747
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            748..882
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1000
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1269
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1468
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1578
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1713
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1906
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2082
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2219
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2370
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2371..2539
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2694
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2695..2818
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2819..2964
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2965..3098
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..3200
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            3201..3292
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            3293..3951
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3932..3937
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /note="hexamer: AGTAAA"
     polyA_site      3951
                     /gene="Atp12a"
                     /gene_synonym="Atp1al1; cHKA; HKalpha2"
                     /note="major polyA site"
ORIGIN      
        1 tgtaacgcca acatcgtcgc tgtcaccact cagggacccg gacctgcaca ccgccgcggc
       61 agccgccacc gaaagatcgg acctgtctct aggaaccaca gtccagcgcg ccgctaaggc
      121 aacgcgcgtc ctcatactta ccgggctcac ctcagcacgc cagcatccgg tgtctttcat
      181 catttgccag cgggttggac caccagtcac agtatgcgcc ggaaaacaga aatctattct
      241 gtagagctca atggaactaa agacgtcgag ctggcagacc agaaagatga caaaaagttc
      301 aagggcggga agaacaaaga ctcagaaccc aacaagagtc aggaggagga gctgaagaaa
      361 gaacttgatc tggatgacca cagactcagt aatacagatt tggaacagaa atatggtaca
      421 aacatcatcc agggtctctc cagtatcaga gctgcagagc tcctggctcg ggatgggccc
      481 aacgccctca cccctcccaa gcaaactcca gagatcatca agttcctcaa acagatggtg
      541 gggggctttt ccatccttct gtggataggt gcagccttgt gctggatcgc atatgtgatc
      601 cagtatgtca gcagtactgc atccctggac aatgtctatt tgggcgccat acttgtcctg
      661 gtcgttattt taacggggat ttttgcttat taccaagaag ccaaaagtac caacattatg
      721 gcaagcttta gtaagatgat cccccagcaa gctcttgtca tccgagatgc agaaaagaag
      781 atcattccag cagagcagct ggtggtgggg gacgttgtgg agattaaagg aggggaccag
      841 atccctgcag acatcagact ggtgttttcc caagggtgca aagtagataa ctcctctctc
      901 actggggagt ctgagcccca ggctcggtca actgagttta cccatgaaaa ccccttggaa
      961 actaagaaca taggcttcta ttctacaacg tgcctggaag gtacggcaac tggcattgtc
     1021 atcaacacgg gtgaccgcac catcatcggg cgcatagcct ccttggcttc aggcgttggg
     1081 agtgagaaga cacccatagc cattgagatt gagcattttg tccatattgt ggcagcagtg
     1141 gctgtgtccg ttggcgtcat tttcttcatc accgccgtgt gcatgaagta ctacgtcctg
     1201 gatgccatca tcttcctcat tagcatcatt gtggccaatg tgccagaggg cctcctggct
     1261 actgtcactg ttaccctgtc actgacggca aagcggatgg ctaagaagaa ctgcttggta
     1321 aagaacctgg aggccgtgga gacgcttggc tccacctcca tcatctgctc agataagaca
     1381 gggaccctca cccagaacag gatgactgtg gcccacttgt ggtttgacaa ccagatcttt
     1441 gtggctgata caagtgaaaa ccagacaaaa caagcctttg accaaagctc tggaacctgg
     1501 gcttccttgt ccaagataat aacactgtgt aaccgagcag agttcaggcc aggccaggag
     1561 agtgtcccca tcatgaagag agttgtggtt ggagatgcct cagaaactgc tctgctaaag
     1621 ttctcagagg ttattttggg tgatgtgatg gacattagga aaagaaacca caaagtagct
     1681 gagattcctt ttaattctac caacaaattt cagctctcca tacatgagac cgaggacccg
     1741 aatgacaagc gcttcctcat ggtgatgaaa ggggcccctg agaggatctt agagaagtgc
     1801 agcaccatca tgatcaacgg ccaggagcag ccactggaca agagctctgc cgatgccttc
     1861 cacacggcct acatggagct gggtggtctg ggagagcgtg tgttgggttt ctgtcacctc
     1921 tacttgccag cagataagtt tccccaaagc tacacatttg atgtagactc cataaacttt
     1981 cccacttcca atctctgctt tgtggggctt ctgtccatga ttgaccctcc tcggtctacc
     2041 gtgccagatg cggtctccaa atgtcggagt gcggggatca aggttatcat ggtcacaggt
     2101 gatcacccta tcacagccaa agctattgcc aagagtgtag gaatcatatc agccaacaat
     2161 gagacggtgg aagacatcgc aaaacgccgc aacattgctg tggagcaagt caacaaaaga
     2221 gaagcgaaag ccgctgtggt gactggcatg gaactgaagg acatgacccc agaacaactg
     2281 gatgagcttc taatcaacta ccaagaaatt gtgtttgccc ggacatcacc ccagcagaag
     2341 ttgatcattg tagaaggctg tcagaggcag gatgcagttg ttgctgtgac gggtgatgga
     2401 gtaaacgact ctccagcatt aaagaaggca gacatcggga ttgctatggg gatagcaggt
     2461 tctgatgctg ctaagaatgc agctgacatg gtcttgctcg atgacaactt tgcatctata
     2521 gtcacagggg tggaggaagg tcgcctgatc ttcgacaacc tgaagaagac cattgcttac
     2581 accctgacaa agaacatcgc tgagctctgc ccttttttga tctacattgt tgctgggctc
     2641 cccctgccta ttggcaccat cactatccta ttcatcgacc tgggcacaga cattatcccc
     2701 tccattgcct tggcctatga gaaagcggaa agtgacatta tgaataggaa acctcgccac
     2761 aagaagaaag acagactggt gaacaagcag ctggccatct actcttacct tcacattggt
     2821 ctcatgcaag ccctgggagg tttcctcgtc tatttcactg tctatgcaca gcagggcttt
     2881 tggcccacct ctctcatcaa cctgagggta tcatgggaaa cagatgacat aaatgacttg
     2941 gaagatagct acgggcagga atggacgagg tatcagagaa aatacctgga atggacaggc
     3001 tcaacggctt tctttgttgc catcatggtc caacaaattg cagatctgat catcaggaaa
     3061 acccggagga attccatctt ccagcagggg ctcttcagaa acaaagtcat ctgggtgggg
     3121 atcatctcgc agatcatcgt tgcgttggtc ctctcctacg gtctcgggag tgtcacagct
     3181 ctgagcttta ccatgctcag ggctcagtac tggtttgtgg ctgtcccgca tgccattttg
     3241 atttgggtgt atgacgagat gcggaagttg ttcatcaggc tctaccctgg aagctggtgg
     3301 gacaagaaca tgtattatta agaccatgtc actcacaggt ctcccagcgg gatatcagag
     3361 atgttcttct tcatgtctgg ctactcctca ggagatctct gcacaatgtg aacactaccg
     3421 aaccccatac gagagagact tttctacaga aaactgtgtg aagcatactt agcttttgta
     3481 ttttaaagat tgagatttaa ctgtttatat agaattttca tctctatgtc tgcctcctta
     3541 ttttaaatta tgtgaagttc agatgtcagc acagggaaga atatatttat acgtatatca
     3601 aggtcactgt gttaaatacc cttgtgtgtc caaggagtgt gctggggtct gccttaagct
     3661 aggctgtgat tcctcaggcc tcactttctt accctcacag agacccagca gaccccctaa
     3721 atccaccaaa tgaacttttt gtttgctgga gtttaacctc aagcaaagag taccaagtga
     3781 cttaagtgct aatatttatt tctaggaaaa cattgccgag ccctcgccag caatcattgt
     3841 atgtcagtca tgttttgtgt tgtaatcgtt agatgtattc atgtgtggca tgtatgacta
     3901 tgttgacagg tccagtgagg ccttgttttt aagtaaaaga tgttttctat aaaa
//
