LOCUS       NM_178050               3394 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus atlastin GTPase 2 (Atl2), transcript variant 2, mRNA.
ACCESSION   NM_178050
VERSION     NM_178050.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Le Guillou,S., Laubier,J., Pechoux,C., Aujean,E., Castille,J.,
            Leroux,C. and Le Provost,F.
  TITLE     Defects of the endoplasmic reticulum and changes to lipid droplet
            size in mammary epithelial cells due to miR-30b-5p overexpression
            are correlated to a reduction in Atlastin 2 expression
  JOURNAL   Biochem Biophys Res Commun 512 (2), 283-288 (2019)
   PUBMED   30879769
  REMARK    GeneRIF: the increase in lipid droplet size observed in the mammary
            epithelial cells of transgenic mice might result from changes to
            lipid droplet formation and secretion because of direct
            modifications to Atl2 expression and indirect changes to
            endoplasmic reticulum morphology resulting from the overexpression
            of miR-30b-5p
REFERENCE   3  (bases 1 to 3394)
  AUTHORS   Nakano,Y., Wiechert,S. and Banfi,B.
  TITLE     Overlapping Activities of Two Neuronal Splicing Factors Switch the
            GABA Effect from Excitatory to Inhibitory by Regulating REST
  JOURNAL   Cell Rep 27 (3), 860-871 (2019)
   PUBMED   30995482
REFERENCE   4  (bases 1 to 3394)
  AUTHORS   Zhao,G., Zhu,P.P., Renvoise,B., Maldonado-Baez,L., Park,S.H. and
            Blackstone,C.
  TITLE     Mammalian knock out cells reveal prominent roles for atlastin
            GTPases in ER network morphology
  JOURNAL   Exp Cell Res 349 (1), 32-44 (2016)
   PUBMED   27669642
  REMARK    GeneRIF: Endoplasmic reticulum morphology is markedly disrupted in
            ATL1, ATL2, and ATL3 knockout cells.
REFERENCE   5  (bases 1 to 3394)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 3394)
  AUTHORS   Nakano,Y., Jahan,I., Bonde,G., Sun,X., Hildebrand,M.S.,
            Engelhardt,J.F., Smith,R.J., Cornell,R.A., Fritzsch,B. and Banfi,B.
  TITLE     A mutation in the Srrm4 gene causes alternative splicing defects
            and deafness in the Bronx waltzer mouse
  JOURNAL   PLoS Genet 8 (10), e1002966 (2012)
   PUBMED   23055939
REFERENCE   7  (bases 1 to 3394)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 3394)
  AUTHORS   Liu,Y. and Hogan,B.L.
  TITLE     Differential gene expression in the distal tip endoderm of the
            embryonic mouse lung
  JOURNAL   Gene Expr Patterns 2 (3-4), 229-233 (2002)
   PUBMED   12617806
REFERENCE   9  (bases 1 to 3394)
  AUTHORS   Ingley,E., Williams,J.H., Walker,C.E., Tsai,S., Colley,S.,
            Sayer,M.S., Tilbrook,P.A., Sarna,M., Beaumont,J.G. and Klinken,S.P.
  TITLE     A novel ADP-ribosylation like factor (ARL-6), interacts with the
            protein-conducting channel SEC61beta subunit
  JOURNAL   FEBS Lett 459 (1), 69-74 (1999)
   PUBMED   10508919
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK008381.1, AK038697.1, AK135988.1, BC060501.1, AK032050.1 and
            BM938279.1.
            
            On Nov 13, 2013 this sequence version replaced NM_178050.3.
            
            Transcript Variant: This variant (2) lacks an internal exon in the
            5' region, which results in translation initiation at a downstream
            start codon, compared to variant 1. The encoded protein (isoform 2)
            has a shorter N-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.278283.1, AK135988.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164142
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AK008381.1         2-83
            83-112              AK038697.1         11-40
            113-366             AK135988.1         3-256
            367-2682            BC060501.1         517-2832
            2683-3085           AK032050.1         3279-3681
            3086-3394           BM938279.1         81-389
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 50.3 cM"
     gene            1..3394
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /note="atlastin GTPase 2"
                     /db_xref="GeneID:56298"
                     /db_xref="MGI:MGI:1929492"
     exon            1..215
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            216..350
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            351..455
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     CDS             366..1604
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ADP-ribosylation factor-like 6 interacting protein 2;
                     ARL-6 interacting protein-2; atlastin-2; ADP-ribosylation
                     factor-like protein 6-interacting protein 2;
                     ADP-ribosylation-like factor 6 interacting protein 2"
                     /codon_start=1
                     /product="atlastin-2 isoform 2"
                     /protein_id="NP_835151.2"
                     /db_xref="CCDS:CCDS37701.1"
                     /db_xref="GeneID:56298"
                     /db_xref="MGI:MGI:1929492"
                     /translation="MDTQGAFDSQSTIKDCATVFALSTMTSSVQVYNLSQNIQEDDLQ
                     HLQLFTEYGRLAMEEIYQKPFQTLMFLIRDWSYPYEHSYGLEGGKQFLEKRLQVKQNQ
                     HEELQNVRKHIHNCFSNLGCFLLPHPGLKVATNPSFDGRLKDIDEDFKRELRNLVPLL
                     LAPENLVEKEISGSKVTCRDLVEYFKAYIKIYQGEELPHPKSMLQATAEANNLAAVAG
                     ARDVYCKSMEQVCGGDKPYIAPSDLERKHLDLKEVALKQFRSVKKMGGDEFCRRYQDQ
                     LEAEIEETYANFIKHNDGKNIFYAARTPATLFAVMFAMYIISGLTGFIGLNSIAVLCN
                     LVMGLALTSLCTWAYVKYSGEFREIGTMIDQIAETLWEQVLKPLGDNLMEENIRQSVT
                     NSIKAGLTDQVSHHARLKTD"
     exon            456..506
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            507..563
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            564..656
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            657..795
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            796..923
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            924..980
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1052
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1484
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..3373
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2675..2680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /note="hexamer: ATTAAA"
     polyA_site      2700
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /note="major polyA site"
     regulatory      3351..3356
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
                     /note="hexamer: AATAAA"
     polyA_site      3373
                     /gene="Atl2"
                     /gene_synonym="2010110I21Rik; Aip-2; Arl6ip2"
ORIGIN      
        1 gctaggaccc gcaggccgcc ccgccggctc ccgggaggtt gataaagcgg cggcggcgtt
       61 tgacgtcagt ggggagttaa ttttaaatcg gtacaagatg gcggaggggg acgaggcagc
      121 gcgaaggcag cagccgcagc aggggctgag gcgccggcga cagaccagcg actcgagcgt
      181 cggagttaac cacgtctcgt ctacgacctc cctaggattc tcaaagctgg attggtggaa
      241 acaatgaacc cttgactggc tttacgtggc gaggtggttg cgaaagagaa acaacaggca
      301 tacaagtttg gaatgaagtg tttgtgattg acagacctaa tggaacaaaa gtggctgtgc
      361 tgctaatgga tacccagggt gcctttgata gccagtcaac cattaaagac tgtgcgacag
      421 tgtttgctct gagcactatg accagctctg tgcaggtata taatttgtct cagaatattc
      481 aagaagatga tcttcaacat ctacagttat ttacagagta tggaagactt gcaatggaag
      541 agatctacca gaagccattt cagacgctaa tgtttttgat tcgagattgg agttatcctt
      601 atgagcactc atatggcttg gaaggtggga agcagttcct tgagaagaga ttacaggtga
      661 agcagaacca gcacgaggag ctgcagaacg tcaggaagca catccacaac tgcttctcaa
      721 acctgggctg cttcctcctc ccgcaccccg gcctcaaagt cgccaccaac cctagctttg
      781 atggacgact gaaagatatc gatgaagact ttaaacggga gcttcgaaat ctggttccat
      841 tgctgcttgc tcctgaaaat cttgttgaga aagagataag tggatcgaaa gtcacttgta
      901 gagatcttgt agagtatttt aaggcataca ttaaaatata tcaaggagag gaactaccac
      961 acccaaagtc tatgcttcag gcaacagctg aggctaataa cctggcagca gtagcaggag
     1021 caagagatgt ctattgtaaa agtatggagc aggtatgcgg tggtgataag ccgtacattg
     1081 caccttcaga tttggagcga aaacacctgg atctcaagga agtggcgctc aaacagtttc
     1141 gttctgtgaa aaaaatgggt ggagatgaat tctgccgccg ttatcaggac cagcttgaag
     1201 ctgaaatcga agaaacctat gcaaatttta taaagcacaa tgatggcaaa aatatcttct
     1261 atgctgctcg cacccctgcc acactgtttg cagtcatgtt tgctatgtat atcatctcag
     1321 gactgactgg cttcattggc ctaaactcta tagctgtctt atgcaacctc gtcatggggc
     1381 tagcactgac gtccctttgt acttgggcat atgttaagta ctctggggag ttcagagaaa
     1441 tcggaacaat gattgatcag attgctgaaa cactgtggga acaggtattg aagcccctgg
     1501 gtgataattt gatggaggaa aacataaggc agtctgtaac aaactctatc aaagcaggcc
     1561 tgactgacca ggtgtctcat catgcccgat taaagacaga ctgacagttc atctgctcat
     1621 ggactccact ctcccttgtt catgcttgct gtacaatgag aactcagata aaataaacca
     1681 aagtttacaa tcaactgtag aagtagttta gtgtaactgg cttcacagat ggccgccaca
     1741 gagtgtgaac gttgtttgtt ggtttgcagc cttctttgct gtctccttag actcggtgac
     1801 tgcctgagga gcatcaggtt atcatgcaca cttgtgccat ggttaattct tttctttctt
     1861 tttaccgaat tcaatgttag tgaaatttgt cttatgtaaa aggatatttc agggaaatat
     1921 tttaagaaat ctatttagag tctctttaat atagtgtccc attgaaattt taatttttag
     1981 agaatttatg aatcactgta tcaagaatca gatgggaatg acaatgaagc ctttaccgag
     2041 ccactacatt aaaagtgtat attgctttac tgccttcaat tgccagtatt acataaatgc
     2101 atgtttcaga aacttcacac aaatcacatg agaactcttg tagctgagaa ggtaattctg
     2161 aggtgtacat ttgtcttgcc tttttaaatt tgtaaacctg ccctgaaagg agatgcatta
     2221 tctgggaaac tgaactgtct ttttgcagtt tagccttcat gtacataaaa tatgccatta
     2281 attttattgg gggaaaaatt ccatccaaaa atgttgccta cagctatgag ttaagagtgt
     2341 ctacagcgtg tagctttcat tttctaaaga ttacaggtag ggcatgtata tgaattataa
     2401 atatataaat acaattttgt attaaaagtt ttgtagttta tggcaaaacc tggttctgtg
     2461 gtaggctact gagtccagtc ctgtgacagg agtgtttgtg gctcgtgtca ctgtgtgaac
     2521 gcatagacag tgtgaagttt tgatatattt gtgatattta tcttccctga gcactgcagt
     2581 ctcaccccca agagaattca atgggaatgt tttattgtga cttgtcctct gttgcatttt
     2641 aaagttattt cctgtaattt attttcagta cataattaaa aatttgttgt atatataaaa
     2701 cgaaagttgt gattcttttt taaggaattc tttcaagtgt gtatctcacc atataacatt
     2761 gttcatgctg aaagagggtg ggtcagggtg caccgagtta gagctcaagg tattgtactt
     2821 taaagctgag cgtgtaaaaa gttatttttc gtgtcttttt atgtaaggga tattttttgt
     2881 gataatctat ctggggattt aataatttta atcttttctc catctcttcc cccactccca
     2941 tcgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgagaga gagagagaga gagagagaga
     3001 gagagagaga gaatgaatat gtgtgtgttt gcgtgtgggc tatatatgta aagctaccat
     3061 ttttccagta cttataacct cctttacaga gacctattaa atcgctccta tttggttttt
     3121 aagagaattc agatgttgag ttgcatgcat gaaggtctga gtttgctttg attggcacac
     3181 ttccctccca ctctgaccca gtcctacttt atatggattg tatttgtttc cgtctgtggt
     3241 aagcagctgc cttctcatct gttcctacac agaacgaatc catccgaaca gtctctcttt
     3301 ctctgaccac tttgttttat gaatgctgta aattgttttt atgaatgaaa aataaaaagt
     3361 ttgttgtata ctaaaaaaaa aaaaaaaaaa aaaa
//
