LOCUS       NM_028835               3721 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus autophagy related 7 (Atg7), transcript variant 3,
            mRNA.
ACCESSION   NM_028835
VERSION     NM_028835.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Wei,W., Gao,X., Qian,J., Li,L., Zhao,C., Xu,L., Zhu,Y., Liu,Z.,
            Liu,N., Wang,X., Jin,Z., Liu,B., Xu,L., Dong,J., Zhang,S., Wang,J.,
            Zhang,Y., Yu,Y., Yan,Z., Yang,Y., Lu,J., Fang,Y., Yuan,N. and
            Wang,J.
  TITLE     Beclin 1 prevents ISG15-mediated cytokine storms to secure fetal
            hematopoiesis and survival
  JOURNAL   J Clin Invest 135 (3), e177375 (2025)
   PUBMED   39589832
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Austin,M.C., Muralidharan,C., Roy,S., Crowder,J.J., Piganelli,J.D.
            and Linnemann,A.K.
  TITLE     Dysfunctional beta-cell autophagy induces beta-cell stress and
            enhances islet immunogenicity
  JOURNAL   Front Immunol 16, 1504583 (2025)
   PUBMED   39944686
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3721)
  AUTHORS   Courreges,C.J.F., Davenport,E.C.M., Bilanges,B., Rebollo-Gomez,E.,
            Hukelmann,J., Schoenfelder,P., Edgar,J.R., Sansom,D.,
            Scudamore,C.L., Roychoudhuri,R., Garden,O.A., Vanhaesebroeck,B. and
            Okkenhaug,K.
  TITLE     Lack of phosphatidylinositol 3-kinase VPS34 in regulatory T cells
            leads to a fatal lymphoproliferative disorder without affecting
            their development
  JOURNAL   Front Immunol 15, 1374621 (2024)
   PUBMED   39664390
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3721)
  AUTHORS   Das,D., Sharma,M., Gahlot,D., Nia,S.S., Gain,C., Mecklenburg,M.,
            Zhou,Z.H., Bourdenx,M., Thukral,L., Martinez-Lopez,N. and Singh,R.
  TITLE     VPS4A is the selective receptor for lipophagy in mice and humans
  JOURNAL   Mol Cell 84 (22), 4436-4453 (2024)
   PUBMED   39520981
REFERENCE   5  (bases 1 to 3721)
  AUTHORS   Jiang,J.J., Chen,C., Nian,L., Liu,Y., Ma,J., Wang,C.Y. and Li,Z.Y.
  TITLE     [Effects of ATG5 and ATG7 Knockout on Ferroptosis Sensitivity of
            RPMI-8226 Cells]
  JOURNAL   Zhongguo Shi Yan Xue Ye Xue Za Zhi 32 (5), 1444-1449 (2024)
   PUBMED   39479830
  REMARK    GeneRIF: [Effects of ATG5 and ATG7 Knockout on Ferroptosis
            Sensitivity of RPMI-8226 Cells].
REFERENCE   6  (bases 1 to 3721)
  AUTHORS   Fortun,J., Dunn,W.A. Jr., Joy,S., Li,J. and Notterpek,L.
  TITLE     Emerging role for autophagy in the removal of aggresomes in Schwann
            cells
  JOURNAL   J Neurosci 23 (33), 10672-10680 (2003)
   PUBMED   14627652
REFERENCE   7  (bases 1 to 3721)
  AUTHORS   Nemoto,T., Tanida,I., Tanida-Miyake,E., Minematsu-Ikeguchi,N.,
            Yokota,M., Ohsumi,M., Ueno,T. and Kominami,E.
  TITLE     The mouse APG10 homologue, an E2-like enzyme for Apg12p
            conjugation, facilitates MAP-LC3 modification
  JOURNAL   J Biol Chem 278 (41), 39517-39526 (2003)
   PUBMED   12890687
REFERENCE   8  (bases 1 to 3721)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 3721)
  AUTHORS   Mizushima,N., Yoshimori,T. and Ohsumi,Y.
  TITLE     Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the
            conjugation-mediated yeast two-hybrid method
  JOURNAL   FEBS Lett 532 (3), 450-454 (2002)
   PUBMED   12482611
REFERENCE   10 (bases 1 to 3721)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Nishitani,T., Komatsu,M., Yamazaki,H.,
            Ueno,T. and Kominami,E.
  TITLE     Murine Apg12p has a substrate preference for murine Apg7p over
            three Apg8p homologs
  JOURNAL   Biochem Biophys Res Commun 292 (1), 256-262 (2002)
   PUBMED   11890701
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC170088.9, AC122843.2 and AC149276.2.
            
            On Mar 23, 2020 this sequence version replaced NM_028835.4.
            
            Summary: This gene encodes an E1-like activating enzyme that is
            essential for autophagy and cytoplasmic to vacuole transport. The
            encoded protein is also thought to modulate p53-dependent cell
            cycle pathways during prolonged metabolic stress. It has been
            associated with multiple functions, including axon membrane
            trafficking, axonal homeostasis, mitophagy, adipose
            differentiation, and hematopoietic stem cell maintenance.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (3) lacks an alternate exon in the
            5' coding region, resulting in the initiation of translation from a
            downstream start codon, compared to variant 1. Variants 2 and 3
            encode the same isoform (2), which has a shorter N-terminus,
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK170769.1, AK007484.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC170088.9         185086-185109
            25-191              AC170088.9         214933-215099
            192-246             AC170088.9         215607-215661
            247-364             AC170088.9         219899-220016
            365-442             AC170088.9         222121-222198
            443-559             AC170088.9         226360-226476
            560-709             AC170088.9         228029-228178
            710-798             AC170088.9         237582-237670
            799-920             AC170088.9         238990-239111
            921-1011            AC170088.9         243116-243206
            1012-1156           AC170088.9         243884-244028
            1157-1315           AC170088.9         245287-245445
            1316-1510           AC170088.9         248092-248286
            1511-1711           AC170088.9         249463-249663
            1712-1827           AC170088.9         251053-251168
            1828-1903           AC122843.2         75167-75242
            1904-1984           AC122843.2         86768-86848
            1985-2107           AC122843.2         138831-138953
            2108-3721           AC149276.2         25518-27131
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6"
     gene            1..3721
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /note="autophagy related 7"
                     /db_xref="GeneID:74244"
                     /db_xref="MGI:MGI:1921494"
     exon            1..24
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            25..191
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..2140
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /note="isoform 2 is encoded by transcript variant 3;
                     ubiquitin-like modifier-activating enzyme ATG7;
                     autophagy-related protein 7; ATG12-activating enzyme E1
                     ATG7; ubiquitin-activating enzyme E1-like protein"
                     /codon_start=1
                     /product="ubiquitin-like modifier-activating enzyme ATG7
                     isoform 2"
                     /protein_id="NP_083111.1"
                     /db_xref="CCDS:CCDS39598.1"
                     /db_xref="GeneID:74244"
                     /db_xref="MGI:MGI:1921494"
                     /translation="MGDPGLAKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIK
                     GYYYNGDSAGLPTRLTLEFSAFDMSASTPAHCCPAMGTLHNTNTLEAFKTADKKLLLE
                     QSANEIWEAIKSGAALENPMLLNKFLLLTFADLKKYHFYYWFCCPALCLPESIPLIRG
                     PVSLDQRLSPKQIQALEHAYDDLCRAEGVTALPYFLFKYDDDTVLVSLLKHYSDFFQG
                     QRTKITVGVYDPCNLAQYPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVT
                     HSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLK
                     LMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPV
                     RQPLYEFEDCLGGGKPKALAAAERLQKIFPGVNARGFNMSIPMPGHPVNFSDVTMEQA
                     RRDVEQLEQLIDNHDVIFLLMDTRESRWLPTVIAASKRKLVINAALGFDTFVVMRHGL
                     KKPKQQGAGDLCPSHLVAPADLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQ
                     CTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGF
                     LSRFDNVLPVSLAFDKCTACSPKVLDQYEREGFTFLAKVFNSSHSFLEDLTGLTLLHQ
                     ETQAAEIWDMSDEETV"
     exon            192..246
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            247..364
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            365..442
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            443..559
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            560..709
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            710..798
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            799..920
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1011
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1156
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1315
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1510
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1711
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1827
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1903
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..1984
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2107
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..3721
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2316..2321
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /note="hexamer: AATAAT"
     polyA_site      2341
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /note="major polyA site"
     regulatory      3712..3717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
                     /note="hexamer: TATAAA"
     polyA_site      3721
                     /gene="Atg7"
                     /gene_synonym="1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553"
ORIGIN      
        1 gtggttgccg gaagttgagc ggcggctggt aagaacagta gccatggggg accctggact
       61 ggccaagttg cagttcgccc cctttaatag tgccctggac gttggcttct ggcacgaact
      121 gacccagaag aagttgaacg agtaccgcct ggacgaggca cccaaagaca tcaagggcta
      181 ttactacaat ggtgactctg ctggtctgcc cacccgcttg acgttggagt tcagtgcttt
      241 tgacatgagt gcctccacgc ctgcccactg ctgcccggcc atgggaaccc tgcacaacac
      301 caacacactt gaggctttta agacagcaga caagaagctc cttctggagc agtcagcaaa
      361 tgagatctgg gaagccataa agtcaggtgc tgctctcgaa aaccccatgc tcctcaacaa
      421 gtttctgctc ctgaccttcg cggacctaaa gaagtaccac ttctactact ggttttgctg
      481 ccccgccctc tgtcttcctg agagcatccc tctaatccgg ggacctgtga gcttggatca
      541 aaggctttca ccaaaacaga tccaggccct ggagcatgcc tatgatgatc tgtgtcgagc
      601 cgaaggcgtc acggccctgc cctacttctt attcaagtac gatgacgaca ctgttctggt
      661 ctccttgctc aaacactaca gtgatttctt ccaaggtcaa aggacaaaga taacagttgg
      721 tgtgtacgat ccctgtaacc tagcccagta ccctggatgg cctttgagga attttttggt
      781 cctggcagcc cacagatgga gcggcagttt ccagtccgtt gaagtcctct gctttcggga
      841 ccgcaccatg cagggagcta gagacgtgac acatagcatc atctttgaag tgaaacttcc
      901 agaaatggca tttagcccag attgtcctaa agctgttggc tgggagaaga accagaaagg
      961 aggcatgggt ccgaggatgg tgaacctcag tggatgtatg gaccccaaaa ggctggctga
     1021 gtcatctgtg gatctgaatc tcaagctgat gtgctggcga ttggtcccca ccttggactt
     1081 ggacaaggtc gtgtctgtca agtgcctgct gctgggagct ggtaccttgg ggtgtaatgt
     1141 ggctaggaca ctgatgggct ggggcgtcag acatgtcacc tttgtggata acgccaagat
     1201 ctcctactcc aatcccgtga ggcagcctct gtatgaattt gaagattgtc tagggggtgg
     1261 caagcccaag gccctggctg cagcagagcg gctacagaaa atatttcccg gagtgaatgc
     1321 cagagggttc aacatgagca tccccatgcc aggacaccct gtgaacttct ctgacgtcac
     1381 gatggagcag gcccgcagag atgtggagca gctggagcag ctcattgata accatgatgt
     1441 catcttcctg ctaatggaca ccagggagag ccggtggctt cctactgtta ttgcagccag
     1501 caagcgaaag ctggtcatca acgctgcctt ggggtttgat acctttgttg tcatgagaca
     1561 tggcctgaag aaacccaagc agcagggagc cggagacctc tgcccaagcc atcttgtagc
     1621 acctgctgac ctgggctcct cactttttgc caacatccct ggatacaagc ttggctgcta
     1681 cttctgcaat gatgtggtgg ctccaggaga ttcaaccaga gaccggactc tggaccagca
     1741 gtgcacagtg agccgcccag gcctggccgt gattgcaggt gccctggctg tggagctgat
     1801 ggtctctgtc ctgcagcatc ctgagggggg ctacgccatc gccagcagca gtgatgaccg
     1861 catgaatgag cctcccacct cgctgggact tgtgcctcac cagatccggg gttttctgtc
     1921 acggttcgat aatgttcttc ctgtcagcct ggcatttgat aaatgtacag cctgttcacc
     1981 caaagttctt gatcagtacg agcgagaagg attcaccttc ctagcgaagg tttttaactc
     2041 ctcacattcc ttcttagaag acttgaccgg tcttaccctg ctccatcaag agacccaagc
     2101 tgctgagatc tgggacatga gtgacgagga gactgtctga agcaagcaac cacagctcag
     2161 gagtacctgg ccctcagcgc aggactggac cgcaggactg gtgatctggg ccctgccacc
     2221 tccctggtcc tgatctccac atctccaagg acgagggtgt accctctgcc acccagttgc
     2281 accctttcct gtgccatctc accagctctg aactcaataa taaccttggc attgccactg
     2341 atctggggct caggtccttc catgtgcact aatctccccc cccccacaca cacactgttg
     2401 ctgaaggaca ccccaggacc caacatagat cagacaaggc tgtgctagga gcctcaccgg
     2461 tagggcacct gctctgggcc ctgggtagca gtgagtgctg agtttgtagc ctcaagtgtt
     2521 caagtggcac accaagccac cctcccccag ctgtgggcat gctgtgtgcc accctgttcc
     2581 agggatggga gaagctcctg ccacagccct gtactgaaaa gcagggaaga gctctgtagg
     2641 atgggtgtgt ccagctgggc ctagtcaggt gccctcactc acggggttgc tcctggggca
     2701 aggcttgtct tcctcttcac tctgggtggg cccttggcag ctgtggccac ccatcctaaa
     2761 tagatgagct gctcccctcc cacacctgtg caccttcact ggggtctcag gtccagaaca
     2821 gaagcccatg cacggctggc ttagcaggtc tcaggaaggg agactagaga ggaccttggc
     2881 ctaacacaga tgctgcaaca agcggcccta ccatctgtgc aaggctcccc acaagtagcc
     2941 aggcctacct gggcacaggg ccccacagcc cacatgccac cctaggagtc aagagccaca
     3001 cagcctcggt ttaagagcac tttattattg ttcttaaggc tacttttaag tacaaaaaaa
     3061 aaaaaaaaaa aaaaaaaaaa aagatggcct gccaaacctt tttttttttc ttcttccagg
     3121 aaaaaaaaac aggccacaga aaatggtata ttacagattt acaaacacag aggggtcagc
     3181 acacacatag atggcttggt tctggggagg tcgcaagctc ctgcccaagc gggctggcag
     3241 gctcctcagc tgacaaatga ggtatcccac tccagggcac ggcctattcc agataggtca
     3301 gcgcaccagg gacccagcca cccgcactgc catccccccc tcccccaaca gcgacgaccc
     3361 gctcactgac gggtatctga ggcccgttgc ccacagagcc agtccgcggt gcggttgttt
     3421 tcctgtgaca acctcagtag cctgtagaat aacaaactag tggctattaa tgcggacgca
     3481 gtgttgtcat ggagtaactg tgtacctgca cgattacaga cacacctacg acaaaaaaga
     3541 tcaacttttt tcccccaaac aacaacaaca aaaaaatgaa tgattatgat aggaaaggga
     3601 aaattaaata gctacatatc attaacaaat taattgttct tcaaaaaata cctacaaatt
     3661 tctctgtaca ttctttacgc acagcgtaat gatggtcttt aaaattacct atataaaaaa
     3721 a
//
