LOCUS       NM_001270988            8984 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ASXL transcriptional regulator 2 (Asxl2), transcript
            variant 1, mRNA.
ACCESSION   NM_001270988 XM_006515238 XM_011243899 XM_017315229 XM_030246967
            XM_036157637
VERSION     NM_001270988.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Langille,E., Al-Zahrani,K.N., Ma,Z., Liang,M., Uuskula-Reimand,L.,
            Espin,R., Teng,K., Malik,A., Bergholtz,H., Ghamrasni,S.E.,
            Afiuni-Zadeh,S., Tsai,R., Alvi,S., Elia,A., Lu,Y., Oh,R.H.,
            Kozma,K.J., Trcka,D., Narimatsu,M., Liu,J.C., Nguyen,T.,
            Barutcu,S., Loganathan,S.K., Bremner,R., Bader,G.D., Egan,S.E.,
            Cescon,D.W., Sorlie,T., Wrana,J.L., Jackson,H.W., Wilson,M.D.,
            Witkiewicz,A.K., Knudsen,E.S., Pujana,M.A., Wahl,G.M. and
            Schramek,D.
  TITLE     Loss of Epigenetic Regulation Disrupts Lineage Integrity, Induces
            Aberrant Alveogenesis, and Promotes Breast Cancer
  JOURNAL   Cancer Discov 12 (12), 2930-2953 (2022)
   PUBMED   36108220
REFERENCE   3  (bases 1 to 8984)
  AUTHORS   Medina,E.A., Delma,C.R. and Yang,F.C.
  TITLE     ASXL1/2 mutations and myeloid malignancies
  JOURNAL   J Hematol Oncol 15 (1), 127 (2022)
   PUBMED   36068610
  REMARK    GeneRIF: ASXL1/2 mutations and myeloid malignancies.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8984)
  AUTHORS   Farber,C.R., Bennett,B.J., Orozco,L., Zou,W., Lira,A., Kostem,E.,
            Kang,H.M., Furlotte,N., Berberyan,A., Ghazalpour,A., Suwanwela,J.,
            Drake,T.A., Eskin,E., Wang,Q.T., Teitelbaum,S.L. and Lusis,A.J.
  TITLE     Mouse genome-wide association and systems genetics identify Asxl2
            as a regulator of bone mineral density and osteoclastogenesis
  JOURNAL   PLoS Genet 7 (4), e1002038 (2011)
   PUBMED   21490954
  REMARK    GeneRIF: the knockdown of Asxl2 in bone marrow macrophages impaired
            their ability to form osteoclasts.
REFERENCE   5  (bases 1 to 8984)
  AUTHORS   Saferali,A., Berlivet,S., Schimenti,J., Bartolomei,M.S., Taketo,T.
            and Naumova,A.K.
  TITLE     Defective imprint resetting in carriers of Robertsonian
            translocation Rb (8.12)
  JOURNAL   Mamm Genome 21 (7-8), 377-387 (2010)
   PUBMED   20577743
REFERENCE   6  (bases 1 to 8984)
  AUTHORS   Baskind,H.A., Na,L., Ma,Q., Patel,M.P., Geenen,D.L. and Wang,Q.T.
  TITLE     Functional conservation of Asxl2, a murine homolog for the
            Drosophila enhancer of trithorax and polycomb group gene Asx
  JOURNAL   PLoS One 4 (3), e4750 (2009)
   PUBMED   19270745
REFERENCE   7  (bases 1 to 8984)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   8  (bases 1 to 8984)
  AUTHORS   Fisher,C.L., Randazzo,F., Humphries,R.K. and Brock,H.W.
  TITLE     Characterization of Asxl1, a murine homolog of Additional sex
            combs, and analysis of the Asx-like gene family
  JOURNAL   Gene 369, 109-118 (2006)
   PUBMED   16412590
REFERENCE   9  (bases 1 to 8984)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   10 (bases 1 to 8984)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of ASXL2 gene in silico
  JOURNAL   Int J Oncol 23 (3), 845-850 (2003)
   PUBMED   12888926
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK080679.1, AC160138.2, AK036839.1, AC155273.3 and BP763544.1.
            
            On or before Oct 10, 2020 this sequence version replaced
            XM_036157637.1, XM_030246967.1, NM_001270988.1.
            
            Summary: This gene encodes a homolog of the Drosophila Asx gene,
            which interacts with genes involved in axial patterning. Mice with
            mutations in this gene display abnormal patterning of the axial
            skeleton, suggesting a similar function in mice as in Drosophila.
            This gene may also be involved in bone mineral density,
            specifically osteoclastogenesis. [provided by RefSeq, Aug 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC141140.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-387               AK080679.1         27-413
            388-390             AC160138.2         29486-29488         c
            391-1458            AK080679.1         417-1484
            1459-2251           AK036839.1         1324-2116
            2252-2252           AC155273.3         25187-25187
            2253-4598           AK036839.1         2118-4463
            4599-8475           AC155273.3         27534-31410
            8476-8984           BP763544.1         40-548              c
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 1.81 cM"
     gene            1..8984
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /note="ASXL transcriptional regulator 2"
                     /db_xref="GeneID:75302"
                     /db_xref="MGI:MGI:1922552"
     exon            1..304
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     CDS             248..4504
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /note="isoform 1 is encoded by transcript variant 1;
                     polycomb group protein ASXH2 homolog; putative Polycomb
                     group protein ASXL2; additional sex combs-like protein 2;
                     additional sex combs like 2, transcriptional regulator"
                     /codon_start=1
                     /product="putative Polycomb group protein ASXL2 isoform 1"
                     /protein_id="NP_001257917.1"
                     /db_xref="GeneID:75302"
                     /db_xref="MGI:MGI:1922552"
                     /translation="MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLK
                     EIRSGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESS
                     DGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSHPPSPPSGCPSPTIPASKVIS
                     PSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVPAKPALWEG
                     KQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVET
                     PDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEF
                     FTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDS
                     QKLTASSSDPKAKKTPAEQPKSILPSEASPVRIVPVVPQSECKEEAVQIPSPSQKEEN
                     QDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLEQKPVACAEQESEKENHVT
                     TTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVTDQIQES
                     LKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPV
                     SRISPMLFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAA
                     AAASVGGTIPGPGPGGGQSPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGG
                     TRELLPCGPQPETNMPGQAQPPGISGAQLQQTSSVPTGLASSGACTSVPLPAHIEISN
                     SEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGASTVYFVADGTVEPKAG
                     SNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSSTLPA
                     ASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTV
                     PLTTKEEKGIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTA
                     KDRKDPCIDTHQYREGLSKTTQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLED
                     ISTSQKFMLGFAGRRTSKPAMAGHYLLNISTYGRGTENIKRTHSVNPDDRFCLSSPTE
                     ALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWASQSSGRHPHHGEASS
                     TNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAVRG
                     KTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSS
                     DMDHNSAIPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIED
                     TPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR"
     exon            305..387
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            388..390
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            391..499
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            500..647
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            648..763
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            764..887
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1031
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1195
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1292
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1398
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..2110
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..8984
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4581..4586
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /note="hexamer: AATAAA"
     polyA_site      4619
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /note="major polyA site"
     regulatory      8954..8959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /note="hexamer: AGTAAA"
     regulatory      8965..8970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /note="hexamer: ATTAAA"
     polyA_site      8977
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
     polyA_site      8984
                     /gene="Asxl2"
                     /gene_synonym="4930556B16Rik; mKIAA1685"
                     /note="major polyA site"
ORIGIN      
        1 agtaagaacc caatatggca gcggcggtag cagtggcagc ggcagcagga ccggccaccc
       61 cgtagagccc cctacccccg ccactgcgcg cctccgcggc cgcttgctcg ctgagttgct
      121 tctcgggtcc gacttcccct ttctcccacc tttcctggca ccgcgggaaa agcagtgcag
      181 ggcgcagcag gcgagggaac gcagccggag ccggggcacg gaagcctccc agtcagtcag
      241 aaccgacatg agggaaaagg gccgtaggaa gaagggcagg acctgggcgg aggccgccaa
      301 gacggtctta gaaaaatacc ctaatacacc catgagtcat aaagaaattc ttcaggttat
      361 ccagagagaa ggactgaaag aaatcagaag tgggacttcc cctcttgcat gcttgaatgc
      421 aatgctgcac acaaactccc gaggagaaga aggcatcttc tataaagttc caggtagaat
      481 gggcgtgtat actttgaaga aagatgtgcc agatggagtg aaagagctgt cagaaggttc
      541 agaagaaagc agtgatggtc agtcagattc tcagagctct gagaacagca gcagcagtga
      601 tggtggcagc aacaaagaag ggagaaagag caggtggaaa aggaaagtat catctagact
      661 gtcacatcca ccctctcccc cgtcaggctg cccatcaccc accattccag caagtaaagt
      721 catttctcca tcacagaagc acagcaaaaa ggcattaaaa caggccttga agcagcaaca
      781 acagaagaag aagcagcagc aatgcagacc aagcatgtcc atctccaatc aacacctctc
      841 tctcaagact gtcaaagcag ccagtgactc tgtacctgcc aaacctgccc tatgggaagg
      901 aaagcaatct gatggacagt caagcagtcc ccagaactca aattccagct tttcttcctc
      961 agtgaaagtg gaaagttctc tgctaggctt ggggaagaag tctttccaga ggtctgacag
     1021 actccacaca agacagatga aaaggactaa atgtgctgac attgatgttg agactcctga
     1081 ttccattctg gttaatacta atcttcgagc actgatcaac aagcacacct tttcagttct
     1141 tcctggagac tgccagcagc gcctgctttt gttgcttcca gaggtggatc gacaggttgg
     1201 tccagatggc ctgatgaagt tgaatggctc agcccttaac aatgagttct tcacttctgc
     1261 agcccagggc tggaaggaaa ggctctcaga aggtgaattt actcctgaaa tgcaggtgag
     1321 aattcgacaa gagattgaga aggagaagaa agtagagctg tggaaggaac aattctttga
     1381 aaattactat ggtcagagtt ctggcctaag ccttgaagat tcacagaaac tgacagcttc
     1441 ctcaagtgat cccaaagcaa agaaaacccc agctgagcaa ccaaaatcca tacttccttc
     1501 agaggcctct cctgtcagaa ttgtcccagt agttcctcag tccgagtgta aagaagaagc
     1561 agtgcagata ccatcaccat cccaaaagga agaaaatcaa gacgaggcga ggccagactc
     1621 caaatcccca gaacctgttc ttgcatcagc tagcaataca aatgagctta tcaccatgaa
     1681 acccatcaag agccctaagg atgagggtct cttagagcag aagcctgttg cctgtgctga
     1741 acaagaatct gagaaagaaa atcatgtaac cacaacttct cgtaataaca aaagtgagaa
     1801 ccaagaagcg ttagctatat ccccaagcaa atctaaaaat gctgggttac aaaaacccat
     1861 aataaagcct gttgcagaag ccagtccact gaaccctgat atgaagatgc caccagcaac
     1921 tgttactgat cagatccaag aaagtctcaa gaggaaatct tctcttactg atgaagaggc
     1981 caccagtagc tgggagaaaa gaccacgtat cactgagaat cgccagcacc agcagccatt
     2041 tcaggtctcc ccacagcctt ttcttaatag aggggacagg gtccaagtgc gaaaagtccc
     2101 acctctcaag atccctgtct ccagaatctc cccgatgctg ttttctacat cacaggtctc
     2161 tcccagggct cgttttccaa tctccatcac tagtccttac agaacaggag ccagaactct
     2221 ggcagacatc aaagcaaaag cccagttggt caaagcacaa aaggcagcag ccgcagcagc
     2281 tgctgcagct gctgcagccg cctcagttgg agggaccatt ccgggacctg gccctggggg
     2341 tggacaaagt ccaagagagg gtggtgaaag gaaaattgct ggaggaggaa gtgcaggctc
     2401 agacccagtc agtacaaatg gaaaaggtcc cacactggaa ctggccggaa ctggaagcag
     2461 gggaggtacg agagaacttt taccctgtgg tcctcaacct gagaccaata tgccaggcca
     2521 agcacagcct cctggtatct ctggagcaca actacagcaa acctcctcag tgcctacggg
     2581 acttgccagc agtggagcat gcacaagtgt cccattacca gcccacatag aaatatcaaa
     2641 cagtgaaaaa ccaaacctcc acaaggcaac agccacagca gcctctcctt gccacttgca
     2701 agaccctagg agttgcagac ttgaaaaagc tctgtctcca acagggcctc ctctgatctc
     2761 aggagcctca actgtttatt ttgtagctga tggcacagtt gagcccaaag caggttctaa
     2821 taagaatgca ccaaagcctt cagccttagc aaagacaact gctcctgctc cactagatat
     2881 gacttcctct cctgtgacaa cagccagttt agaaaaactc cctgtacccc agatcagtgg
     2941 aactgcaaca tctactggat cagctccatc ctcaagcact ttgccagcag cttctagcct
     3001 taaaacccca ggaacttctg caaatatgaa tggacccatt tcaagaacaa gttctagtat
     3061 ccctgctaat aatcctttag ttactcagct gctccagggc aaagatgttc ccttggagca
     3121 aattctgcct aagcctctca ccaaaattga aatgaaaaca gttccactga ctacaaaaga
     3181 ggaaaaaggc atagggatat tccctggtat cagtgtaatg gaaagtagca gcagagagga
     3241 agttaatggc aggcaggccc atctggctat cccgcagttg gggaaaccct tgcaaagtaa
     3301 acagctttcc caggttccaa ggccagtttt cactgctaag gaccggaagg acccctgcat
     3361 tgacactcac cagtaccgag aaggtctaag taaaacaacg caagatcagc tctttcagac
     3421 tcttatccag agggctcaga gacagagtgt tctttcattt gtaccgccct ctcagttcaa
     3481 ctttgctcac tcaggtttcc acttggaaga catctccaca agtcagaagt tcatgctggg
     3541 ttttgctggc agaaggacat ccaagcctgc aatggcaggt cactatttac ttaatatttc
     3601 cacctatggc aggggtacag agaacattaa aaggacccat tctgtaaatc ctgatgatcg
     3661 attttgtcta agtagcccca ctgaggcgtt gagaatgggg catgcagatt ataaaaacac
     3721 aactggagaa atcagcagca aagaagatga aagtgatgaa gatcgcgtag gtgatgaaca
     3781 ggagcctatt tcagtgaagg aggagccatg ggcttcccag agttctggca ggcatccgca
     3841 ccatggggaa gcttcatcta ccaatgattg tttagctagc aagaatggta agactgaggc
     3901 accagtgagt gagcaaacca ctttaggcca ggagaattac atattctcta gaggccaagc
     3961 atctgatgaa aagagcctgc ctagagattt tattccggca gcacataaac agatgactca
     4021 tgctgtgaga ggtaagacag tgtgtagcag ccctgagctt ttcaattcta ctgctctttc
     4081 tcttcccgca gacagtccca cacatcagcc tctactcctt ccaccactgc aaacccccaa
     4141 attgtatgga agccccacac agatagggcc aagctataga ggcatgatca atgtctccac
     4201 ctcatcagac atggaccata actctgctat accaggcagc caggtatcta gcaatgtagg
     4261 ggacgtcatg tcgttttcag tgactgtcac taccatccct gccagtcaag caatgaatcc
     4321 cagcagccat ggccagacaa ttcctgttca gacttttcct gatgacaata gtatcgagga
     4381 cacaccatca aaatgttact gccggttgaa agccatgatc atgtgcaaag gatgtggagc
     4441 tttctgccat gatgattgca ttggcccctc caaactctgt gtctcctgcc ttgttgttcg
     4501 gtaataagac tgtagcgtat gtaaaggagg gaagggaagg cttaaaaagt catgttcttg
     4561 cacccttttt aaatcacaga aataaagttt cagttgtctc aagagacatt aatcaggaat
     4621 acagaatgca ggtcttcaca tttacaggaa gagcaccttg tatagtaggt ctgagtcaaa
     4681 taggactgaa ttggtgacaa agtttgcact tacaaaatca tgtaaatatg tcacattttg
     4741 ttttaacatt tttttcaaag tatttaggta atgatgtatt aacttcacat tcagatttat
     4801 gttccatttc ttttgactga gaaaagttga acggtatgca tggtgaaagg aagctgtctg
     4861 cctctgacct ttccccaggc tatgaaaagg aaagtgaggg agcgatcata gtgctgatta
     4921 tgtcttcaca gactggctca ggttcttcac gaagaagatg gcctttgtgg gatttaattc
     4981 ggggtttgct catcccttta ctttaggtgg aactattttc agtgaaattt tcaaaaggta
     5041 ttaacagcac agacagtgtt tttgaccctg acagccctgg cttagctttt ggcttccatg
     5101 aatcagtact gttgttaaaa gcagagctct gctgttaaga ggttctgttt ctaagggact
     5161 gctgagttag attagtatat acgtggcagt agttcagcaa taatacatca caacattgca
     5221 tcaatcatgg gctcccacca ataaacactt tacgggtggg tgtgtgtgtg tgtgcgcgcg
     5281 cgcgcatgtg cgcatactgt gtaatgttgg aaaagggatg aaattgaaga aactgaaact
     5341 tcacggaatt tggaagagtg gttttaacat gggacagaaa atagtctttt cctattctct
     5401 caatccattt ttaggtagaa agggtcttaa gtgatactta tgctttggca tccagagcat
     5461 aagagatgaa tgcagctacc ctgtgccatc ctactataat aaattttgca cccacacaca
     5521 agtacacagt ttgctgtttt agaaagtgat tctacaatct cggctgtcag gacagctggg
     5581 atgcttactg tgaaagggtc ttgtgagtga ctaatggaat atatggctgt gagattttgc
     5641 tgtttttctt tccttttgat ttttttttaa tcctcccagt actccagaga cagagacaga
     5701 ccagtctctg agttcaaggc cagcctgatc ttcataatga gctccagaca accagggcta
     5761 tctaagaagc cttgtctcaa aacaaaaata atcacaaaaa ccctaaataa ataaaagaag
     5821 taaaaaaaaa ccacacacac acaaggagct ggagagatgg ctcagtggtt acgagcactg
     5881 actgctcttc tagagatcct gagttcaatt cccagcaacc gcatggtggc tcacaaccat
     5941 ctgtaatggg atctgatgcc ctcttctagt gtgtctgaag acagtgacag tgtactcata
     6001 tacataaaga aatctttttt taaaaaagag agaaaagaaa aactagttgg tggacatggt
     6061 gggtgacacg cctttaatct cagcacttgg gaggaagagg caggtgaatc tctgggttag
     6121 gaccagtatg gtttgcatag caagttccag agtacacagt gaaacacttg tcttttttaa
     6181 aaaacaaaag ctagctacga atttttaaag aaatactgtt taagttagag atggtttaaa
     6241 gaaaaatagt tcaccaaaat ttagtattat atagtctgta ttgtttggtt tcaagtctcc
     6301 taatttgaag tatgtggtgt aatattgcaa cccagtatag gtgtatttca aaagctattt
     6361 ataaaactca gaacaattta acttgctaat tatatcacct tccagattgg agaaaggaga
     6421 cagtaaggta ttttgcctgc cttgtgatgc cctcccctga ccaccctacc tttttgcatc
     6481 aaacactggg aatgcttcct cagtccctgc tccaaactat gaagctcacc cttcattttt
     6541 cctccctgct attgtggacc taagcaatta cagtttaaat tctgtataca gcactagtca
     6601 agacaatgtg atatccttcc accttccccc tttatttcaa gaaatgcata gataagggag
     6661 aagaaaggct tcaaggattc atggttaaga agtgtgtatg ttctgaagtg aaggaccagt
     6721 aagaaataca agccaggccg ggcggtggtg gcgcacacct ttgatcccag cacttgggag
     6781 gcagaggcag gcggattttt gagtttgaga ccagcctgat ccatagagtg agttccagga
     6841 cagccaggtt tatacagaga aaccctgtct tgaaagagcc aaaaaaaaaa aaaaaaaata
     6901 caagccagga ttctgctccc aaaccttcag gacaatgggt agtccccatg ggaccttaaa
     6961 agacccatag ctgagtagtc tcccttgtct accctgcttt aagagggtga atgacggaat
     7021 agactttatg gtgtcagtga gtagtttgta ttagaataat tttcctattt cctaccttgc
     7081 tttctagaaa ggcttttgct ccatacttcc cttaatctca gaatggatgg aaaagaggaa
     7141 gacattaggg actgtcaggt ccatttaaag aggaaattac attgttactc ttcttgatct
     7201 cctcaggcta tgtaagtctg aagtactgtt cttctccact aagtaatacc caactaattt
     7261 ccatttaaaa gtattccaaa agcagcattt accaacaatt tttttagatt gaaaatatat
     7321 taaataagaa tccatctagt ctatttgtgt taaaacatgc tttcagatgg aagacgaaca
     7381 ttcatcttat aaatgaacag aaaataaact agtctggccc tttgagtttg tcactccagt
     7441 aactattaac acagattttg tcatggccta tcatttcttt tacctttaac tcaactctga
     7501 actgattgaa taagtgctaa gcttattctt tgggctttgg gtggcaattt tcagtgaggt
     7561 attaaatata tctgatacct gatggggctg tgccccatcc agccctcaaa gtagtctctg
     7621 taaatcgctg ggatagtatt catattgtgt catctcagtg ttatttttaa gtgttctgag
     7681 ttgcctaagt agaacactct ctgaatcata aggacttttt gttttgcttt ttgaataatc
     7741 accattcttt agctcagtaa tatgggggtg ggggagcagc acaaataacc tggagttgtt
     7801 tggccttggt tccttaagtc ctgtgttcaa gacactgaag ctatctccca tgttctgtgt
     7861 gagatttctg agtaagatct ggcaagtgag ggagcttttc ttgagagggg aaggctacat
     7921 ttctatgggt atagaaatgg gaatatttgc gagcctatct cagatgtaca ttaatagacc
     7981 tcactgcctt ccgggtgaag gtgtgttgtc ctacatagaa cggtcccttc agttaccatt
     8041 ctgattgctc tatgctgctt tcctcctctt aaagtttcat gagctcttgg gttaaatggt
     8101 ttgttatgag caggctttcc attactcatt gttctgcgat ttattttaga gattgtccat
     8161 gagcactgaa caaggagaag aagtttcttg ttgctttaca acagtgtgtg ctaagtgctg
     8221 gacttggcca tgggtagtct gcagctcaat ttatagaaca gtccaaagag actttgcaaa
     8281 acatgatagg cagggacctt ttcctctgtg tttcaatacc tatataccta ttgaacaaag
     8341 cagtaggtag acatttccta aagttgaagc agtagcttca tagagtcttg gttactttat
     8401 tttttttaat gctttacatt tgatacaact attgaagatc aatttaaaaa taactttcca
     8461 gtaatatatt ttaagtaata tatattttaa tatctatgta aaaaagtgac agtggaggac
     8521 tgaatttctc atgtatacta tgtttaatgg tagggtctca ctgttaaggc acaaattatt
     8581 tcaaaggagt tgctctaaaa acagattatt tcccggaaaa aaaaaaatgc aataatggga
     8641 atgatttggg gcttttattt tttattttaa aagaatattg acttatttag cacaagctat
     8701 ttttccctct ctggtgataa ggtttgtaca gactggtttg gtaaatgcta aatttttgtc
     8761 ttttgccttt ttaaaggaat tgttaccatt ggaattgagg gtatgtacag agaagttggt
     8821 gtcaatgcca tttaataagt catatttttt cacaaataag gaaaaatcat tttaatgaga
     8881 attttaagta tttgcaataa atatgtgtat atagattgta tgtattcata tattattttt
     8941 cattttatta ttaagtaaaa gataattaaa agtgaaatta aaaa
//
