LOCUS       NM_001291003            2261 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus astacin like metalloendopeptidase (Astl), transcript
            variant 1, mRNA.
ACCESSION   NM_001291003 XM_006499122
VERSION     NM_001291003.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Zeng,Y., Chen,B., Sun,Y., Yang,A., Wu,L., Li,B., Mu,J., Zhang,Z.,
            Wang,W., Zhou,Z., Dong,J., Liu,R., Luo,Y., Sun,X., Sang,Q.,
            Kuang,Y. and Wang,L.
  TITLE     Bi-allelic variants in ASTL cause abnormal fertilization or oocyte
            maturation defects
  JOURNAL   Hum Mol Genet 32 (14), 2326-2334 (2023)
   PUBMED   37133443
  REMARK    GeneRIF: Bi-allelic variants in ASTL cause abnormal fertilization
            or oocyte maturation defects.
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Schmitz,C., Sadr,S.Z., Korschgen,H., Kuske,M., Schoen,J.,
            Stocker,W., Jahnen-Dechent,W. and Floehr,J.
  TITLE     The E-modulus of the oocyte is a non-destructive measure of zona
            pellucida hardening
  JOURNAL   Reproduction 162 (4), 259-266 (2021)
   PUBMED   34320465
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2261)
  AUTHORS   Tokuhiro,K. and Dean,J.
  TITLE     Glycan-Independent Gamete Recognition Triggers Egg Zinc Sparks and
            ZP2 Cleavage to Prevent Polyspermy
  JOURNAL   Dev Cell 46 (5), 627-640 (2018)
   PUBMED   30122633
REFERENCE   4  (bases 1 to 2261)
  AUTHORS   Nozawa,K., Satouh,Y., Fujimoto,T., Oji,A. and Ikawa,M.
  TITLE     Sperm-borne phospholipase C zeta-1 ensures monospermic
            fertilization in mice
  JOURNAL   Sci Rep 8 (1), 1315 (2018)
   PUBMED   29358633
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2261)
  AUTHORS   Korschgen,H., Kuske,M., Karmilin,K., Yiallouros,I., Balbach,M.,
            Floehr,J., Wachten,D., Jahnen-Dechent,W. and Stocker,W.
  TITLE     Intracellular activation of ovastacin mediates pre-fertilization
            hardening of the zona pellucida
  JOURNAL   Mol Hum Reprod 23 (9), 607-616 (2017)
   PUBMED   28911209
  REMARK    GeneRIF: Intracellular activation of Astl mediates
            pre-fertilization hardening of the zona pellucida.
REFERENCE   6  (bases 1 to 2261)
  AUTHORS   Floehr,J., Dietzel,E., Schmitz,C., Chappell,A. and
            Jahnen-Dechent,W.
  TITLE     Down-regulation of the liver-derived plasma protein fetuin-B
            mediates reversible female infertility
  JOURNAL   Mol Hum Reprod 23 (1), 34-44 (2017)
   PUBMED   27733488
REFERENCE   7  (bases 1 to 2261)
  AUTHORS   Burkart,A.D., Xiong,B., Baibakov,B., Jimenez-Movilla,M. and Dean,J.
  TITLE     Ovastacin, a cortical granule protease, cleaves ZP2 in the zona
            pellucida to prevent polyspermy
  JOURNAL   J Cell Biol 197 (1), 37-44 (2012)
   PUBMED   22472438
  REMARK    GeneRIF: Ovastacin is a pioneer component of mouse cortical
            granules and plays a definitive role in the postfertilization block
            to sperm binding that ensures monospermic fertilization and
            successful development
REFERENCE   8  (bases 1 to 2261)
  AUTHORS   Sachdev,M., Mandal,A., Mulders,S., Digilio,L.C., Panneerdoss,S.,
            Suryavathi,V., Pires,E., Klotz,K.L., Hermens,L., Herrero,M.B.,
            Flickinger,C.J., van Duin,M. and Herr,J.C.
  TITLE     Oocyte specific oolemmal SAS1B involved in sperm binding through
            intra-acrosomal SLLP1 during fertilization
  JOURNAL   Dev Biol 363 (1), 40-51 (2012)
   PUBMED   22206759
  REMARK    GeneRIF: Data show that SAS1B/SAS1R (Sperm Acrosomal SLLP1
            Binding/Sperm Acrosomal SLLP1 receptor) bound to sperm acrosomal
            protein SlLP1 with high affinity.
REFERENCE   9  (bases 1 to 2261)
  AUTHORS   Acloque,H., Lavial,F. and Pain,B.
  TITLE     Astacin-like metallo-endopeptidase is dynamically expressed in
            embryonic stem cells and embryonic epithelium during morphogenesis
  JOURNAL   Dev Dyn 241 (3), 574-582 (2012)
   PUBMED   22275110
  REMARK    GeneRIF: ASTL is dynamically expressed in the embryonic epithelium
            and in embryonic stem cells, suggesting an important function for
            the control of epithelial cell behavior during early development.
REFERENCE   10 (bases 1 to 2261)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV649054.1, BC064729.1 and DV662582.1.
            
            On Mar 21, 2014 this sequence version replaced XM_006499122.1.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript but encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064729.1, AJ537599.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DV649054.1         13-20
            9-2245              BC064729.1         1-2237
            2246-2261           DV662582.1         650-665
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 61.93 cM"
     gene            1..2261
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /note="astacin like metalloendopeptidase"
                     /db_xref="GeneID:215095"
                     /db_xref="MGI:MGI:3046414"
     exon            1..116
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1369
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /EC_number="3.4.24.21"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; ovastacin; oocyte astacin; sperm acrosomal
                     SLLP1; sperm acrosomal SLLP1-binding protein; astacin-like
                     metalloendopeptidase (M12 family)"
                     /codon_start=1
                     /product="astacin-like metalloendopeptidase isoform a
                     precursor"
                     /protein_id="NP_001277932.1"
                     /db_xref="CCDS:CCDS71138.1"
                     /db_xref="GeneID:215095"
                     /db_xref="MGI:MGI:3046414"
                     /translation="MGIMGSLWPWILTMLSLLGLSMGAPSASRCSGVCSTSVPEGFTP
                     EGSPVFQDKDIPAINQGLISEETPESSFLVEGDIIRPSPFRLLSVTNNKWPKGVGGFV
                     EIPFLLSRKYDELSRRVIMDAFAEFERFTCIRFVAYHGQRDFVSILPMAGCFSGVGRS
                     GGMQVVSLAPTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINF
                     IKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVC
                     RLYNCSRSVPDSHGRGFEAQSDGSSLTPASISRLQRLLEALSEESGSSAPSGSRTGGQ
                     SIAGLGNSQQGWEHPPQSTFSVGALARPPQMLADASKSGPGAGADSLSLEQFQLAQAP
                     TVPLALFPEARDKPAPIQDAFERLAPLPGGCAPGSHIREVPRD"
     exon            117..242
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     exon            243..304
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     exon            305..398
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     exon            399..516
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     exon            517..698
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     exon            699..780
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     exon            781..935
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     exon            936..2250
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2223..2228
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /note="hexamer: AATAAA"
     polyA_site      2247
                     /gene="Astl"
                     /gene_synonym="7420700F21; Sas1b"
                     /note="major polyA site"
ORIGIN      
        1 ggaggccttt ccctgccaca tttctgtgtg ttgcttgcca agagggcagt gaggacccac
       61 catgggtatc atgggaagcc tgtggccttg gatattaact atgctctcct tgctaggttt
      121 gagcatggga gcaccctcag catccagatg ttctggagtc tgcagtacca gtgttccaga
      181 aggcttcact cctgagggaa gcccggtatt tcaggacaag gacatccccg caattaacca
      241 agggctcatc tcagaggaga ccccagaaag cagcttcctg gtagaagggg acattatccg
      301 gccaagccct ttccgattgt tgtcagtgac caataataaa tggcccaagg gcgttggtgg
      361 ctttgtggag atccccttcc tgctttccag aaagtatgat gaactcagcc gccgggtcat
      421 tatggatgcc tttgctgagt ttgaacgttt cacatgcatc cggtttgttg cctaccatgg
      481 tcagagagac tttgtttcca ttcttcctat ggcggggtgt ttctctggtg tgggacgcag
      541 tggagggatg caggtggtgt ccttggcacc cacttgtctc cggaagggcc gaggcattgt
      601 cctacatgag ctcatgcacg tacttggctt ctggcatgag cattcacggg cagatcggga
      661 ccgctacatc caagtcaact ggaacgagat cctcccgggc tttgaaatca acttcatcaa
      721 gtcacggagt accaatatgt tagttcccta tgactactca tctgtgatgc attatgggag
      781 atttgccttc agctggcgtg ggcagcccac catcatacca ctctggacct ccagtgttca
      841 cattggccag cgatggaacc tgagtacctc agatatcacc cgggtctgca ggctgtataa
      901 ctgcagccgg agtgtccctg actcccacgg gagagggttt gaggcccaga gtgatggaag
      961 cagcctcacc cctgcctcta tatcacgtct acaaagactt ctcgaggcac tgtcagagga
     1021 atctggaagc tctgccccta gtggctccag gactggaggc cagagtattg ccgggcttgg
     1081 taacagccag caaggatggg agcatcctcc tcagagcaca ttcagtgtgg gagccttggc
     1141 aagaccacct cagatgctag ccgatgcttc aaaatcgggg cctggagcag gtgcagacag
     1201 cttgtctcta gagcagttcc agctagccca ggcccccact gtacctcttg ctctatttcc
     1261 agaagccaga gacaagccag cacctatcca agatgccttt gagaggctag ctccacttcc
     1321 aggaggctgt gcacctggaa gtcacattag agaggtgccc agagactgag cctttagact
     1381 tctgtcccca agtggagagg gtgtattctg tctagatcag ctaggtcgtc cagtctttgg
     1441 cccctctggg catcacccct gtcttctatc ccctgcactg gctactctag caggcccagg
     1501 tcacttcctt ggtctctttt cccacaacac ccccaccctg cagagttcct aggggtagag
     1561 aggctctagg ggcttttctt ggcttgagcc ttagccctgg aagatgcttg gcatggagac
     1621 aggatcctgg gagtggccgt caagagcaag gttccagtat tggttgcctc tggaagttct
     1681 tcaactgagg actagctccc agcatggact caagtgctgc cttgggcatg gacctcagtg
     1741 ggaagccctg tggctggaac cttgaatatc ctgctctcca tgatgcctgg ctctaaagat
     1801 gttctgaagc cctgggatta agtcctgttc tccaggaggc agagaacaca cttggttctc
     1861 ctgctaagct ggactagcaa gagatggttc cttatcagct ctgcatggtg gaggccttgg
     1921 caagaccacc agtccctgga cagcagcctt gagtctttgt gaatgtcaga tcactgtttt
     1981 gcccacaagg tgctcaacta gaagccactg agggcaaatg tcacacgact tgtccttcag
     2041 ccatgccata ctcctccttg atctatgagg tttcttctaa gaacaaagca tgtaggtggt
     2101 agggtctgag ccaggagtcc tcaattctcc aggagcctgt tggggggcca ggggagccct
     2161 gcttgggata gaaggggaac cagaaccagc gaggaagggc acttcatgtg ggaaaaggta
     2221 gaaataaagt tgggtgtttg cttcatgcaa aaaaaaaaaa a
//
