LOCUS       NM_001363056            2121 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ASPSCR1 tether for SLC2A4, UBX domain containing
            (Aspscr1), transcript variant 5, mRNA.
ACCESSION   NM_001363056 XM_006534084
VERSION     NM_001363056.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Habtemichael,E.N., Li,D.T., Camporez,J.P., Westergaard,X.O.,
            Sales,C.I., Liu,X., Lopez-Giraldez,F., DeVries,S.G., Li,H.,
            Ruiz,D.M., Wang,K.Y., Sayal,B.S., Gonzalez Zapata,S., Dann,P.,
            Brown,S.N., Hirabara,S., Vatner,D.F., Goedeke,L., Philbrick,W.,
            Shulman,G.I. and Bogan,J.S.
  TITLE     Insulin-stimulated endoproteolytic TUG cleavage links energy
            expenditure with glucose uptake
  JOURNAL   Nat Metab 3 (3), 378-393 (2021)
   PUBMED   33686286
REFERENCE   3  (bases 1 to 2121)
  AUTHORS   Panico,P., Hiriart,M., Ostrosky-Wegman,P. and Salazar,A.M.
  TITLE     TUG is a calpain-10 substrate involved in the translocation of
            GLUT4 in adipocytes
  JOURNAL   J Mol Endocrinol 65 (3), 45-57 (2020)
   PUBMED   32698137
  REMARK    GeneRIF: TUG is a calpain-10 substrate involved in the
            translocation of GLUT4 in adipocytes.
REFERENCE   4  (bases 1 to 2121)
  AUTHORS   Habtemichael,E.N., Li,D.T., Alcazar-Roman,A., Westergaard,X.O.,
            Li,M., Petersen,M.C., Li,H., DeVries,S.G., Li,E.,
            Julca-Zevallos,O., Wolenski,J.S. and Bogan,J.S.
  TITLE     Usp25m protease regulates ubiquitin-like processing of TUG proteins
            to control GLUT4 glucose transporter translocation in adipocytes
  JOURNAL   J Biol Chem 293 (27), 10466-10486 (2018)
   PUBMED   29773651
REFERENCE   5  (bases 1 to 2121)
  AUTHORS   Habtemichael,E.N., Alcazar-Roman,A., Rubin,B.R., Grossi,L.R.,
            Belman,J.P., Julca,O., Loffler,M.G., Li,H., Chi,N.W., Samuel,V.T.
            and Bogan,J.S.
  TITLE     Coordinated Regulation of Vasopressin Inactivation and Glucose
            Uptake by Action of TUG Protein in Muscle
  JOURNAL   J Biol Chem 290 (23), 14454-14461 (2015)
   PUBMED   25944897
  REMARK    GeneRIF: Insulin acts through TUG proteins to control IRAP
            targeting, similar to GLUT4; the activity of vasopressin, an IRAP
            substrate, is reduced in mice with disrupted TUG action in muscle.
REFERENCE   6  (bases 1 to 2121)
  AUTHORS   Yu,C., Cresswell,J., Loffler,M.G. and Bogan,J.S.
  TITLE     The glucose transporter 4-regulating protein TUG is essential for
            highly insulin-responsive glucose uptake in 3T3-L1 adipocytes
  JOURNAL   J Biol Chem 282 (10), 7710-7722 (2007)
   PUBMED   17202135
  REMARK    GeneRIF: insulin-stimulated dissociation of TUG and GLUT4 as an
            important action by which insulin stimulates glucose uptake.
REFERENCE   7  (bases 1 to 2121)
  AUTHORS   Tettamanzi,M.C., Yu,C., Bogan,J.S. and Hodsdon,M.E.
  TITLE     Solution structure and backbone dynamics of an N-terminal
            ubiquitin-like domain in the GLUT4-regulating protein, TUG
  JOURNAL   Protein Sci 15 (3), 498-508 (2006)
   PUBMED   16501224
REFERENCE   8  (bases 1 to 2121)
  AUTHORS   Asakawa,J., Kuick,R., Kodaira,M., Nakamura,N., Katayama,H.,
            Pierce,D., Funamoto,S., Preston,D., Satoh,C., Neel,J.V. and
            Hanash,S.
  TITLE     A genome scanning approach to assess the genetic effects of
            radiation in mice and humans
  JOURNAL   Radiat Res 161 (4), 380-390 (2004)
   PUBMED   15038760
REFERENCE   9  (bases 1 to 2121)
  AUTHORS   Bogan,J.S., Hendon,N., McKee,A.E., Tsao,T.S. and Lodish,H.F.
  TITLE     Functional cloning of TUG as a regulator of GLUT4 glucose
            transporter trafficking
  JOURNAL   Nature 425 (6959), 727-733 (2003)
   PUBMED   14562105
  REMARK    GeneRIF: TUG traps endocytosed GLUT4 and tethers it
            intracellularly, and insulin mobilizes this pool of retained GLUT4
            by releasing this tether
REFERENCE   10 (bases 1 to 2121)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL663030.12 and AY349133.1.
            
            On May 5, 2018 this sequence version replaced XM_006534084.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.2208511.1,
                                           SRR5189685.150430.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-447               AL663030.12        129169-129615
            448-503             AL663030.12        129854-129909
            504-618             AL663030.12        133325-133439
            619-719             AL663030.12        134234-134334
            720-777             AL663030.12        142458-142515
            778-851             AL663030.12        144343-144416
            852-1281            AL663030.12        144726-145155
            1282-1421           AL663030.12        156958-157097
            1422-1503           AL663030.12        157295-157376
            1504-1570           AL663030.12        158298-158364
            1571-1633           AL663030.12        159417-159479
            1634-1686           AL663030.12        159886-159938
            1687-1738           AL663030.12        164472-164523
            1739-1808           AL663030.12        164744-164813
            1809-1984           AL663030.12        164885-165060
            1985-2087           AL663030.12        165158-165260
            2088-2121           AY349133.1         1756-1789
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 84.41 cM"
     gene            1..2121
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /note="ASPSCR1 tether for SLC2A4, UBX domain containing"
                     /db_xref="GeneID:68938"
                     /db_xref="MGI:MGI:1916188"
     exon            1..447
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     CDS             391..1998
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /note="isoform 5 is encoded by transcript variant 5;
                     tether, containing a UBX domain, for GLUT4; tether
                     containing UBX domain for GLUT4; alveolar soft part
                     sarcoma chromosomal region candidate gene 1 protein
                     homolog; alveolar soft part sarcoma chromosome region,
                     candidate 1"
                     /codon_start=1
                     /product="tether containing UBX domain for GLUT4 isoform
                     5"
                     /protein_id="NP_001349985.1"
                     /db_xref="GeneID:68938"
                     /db_xref="MGI:MGI:1916188"
                     /translation="MQQLHKDCRATEWPWTQSSVLEDTCRRQDFNPSEYDLKFQRTVL
                     DLSLQWRFANLPNNAKLEMVPVSRSREGPENIVRIAFQLDDGSRLQDAFCSRQTLWEL
                     LSHFAQTRERLQQLGEKTPVCVYMRNEVTGRAALQNTTLQSLGLTGGSATIRFVIKQC
                     DTAGKQESIAVRSKAPGSPVSSLSADQASSSTLLPLNSGEFSRGDLNHEGDANTSGTG
                     LEGGPKPTDAQTKQSTSEPASAPFVPFSGGGQRLGGPSASLRPLTSPSANSSKSFSGP
                     GGPSKPKKPKPGEEPQQEPEPPVDRDPVVYHPDLEDLLQPWPAEVPDEFFEVTVDDVR
                     RRLAQLKSERKRLEEAPLVTKAFREAQMKEKLERYPKVALRVLFPDRYILQGFFRPSE
                     TVGDLRDFVRSHLGNPELSFYLFIAPPKMVLDDHTLTLFQANLFPAALVHFGAEEPTG
                     LYLEPGLLEHTVSPSTADVLVARCMSRASGSPPLLPAPDPVSLESEPIAEDGALGPPE
                     PIQGTAQPVKRSLGKVPKWLKLPASKR"
     exon            448..503
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            504..618
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            619..719
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            720..777
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            778..851
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1281
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1421
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1503
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1570
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1633
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1686
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1738
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1808
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1984
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2088
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2064..2069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /note="hexamer: AATAAA"
     polyA_site      2088
                     /gene="Aspscr1"
                     /gene_synonym="1190006K01Rik; ASPC; ASPCR1; ASPL; ASPS;
                     RCC17; TUG"
                     /note="major polyA site"
ORIGIN      
        1 atctgggtcc cgggggcggg gcgggatact tggaggcgcg tttgctgagc acggtggggt
       61 ggaccccact aacagggtcg agccttgcga gccccagaat cttcggcgct tgtccgaatc
      121 cagccttctc ggtcaccggg aagagtggcg ttccttcctg ctcagctcag gggcgtccct
      181 ccggacccta gggcctgtgt tcccggaagt gaattttgct aagtcactca cggcttttga
      241 ttgttgctgt ttgtgacctc gtatgaatta cacctgctta ttaaacctct gttgttaggt
      301 tacaaaggcg tacacgattc atatctgtgg cagggccata cagagtgacc tccatttgga
      361 gctgcgctca tgtagaggag ggttctggtc atgcagcagt tgcacaaaga ctgccgggcc
      421 acagagtggc cgtggacaca aagttcagta ctagaagaca catgccggcg gcaagacttc
      481 aaccccagtg aatacgacct gaagtttcag aggacggtgc tagacctctc tctccagtgg
      541 agatttgcca acctgcctaa caatgccaag ctggagatgg tgcctgtgtc ccgtagccgc
      601 gaggggcctg agaacatagt tcgcatcgcg ttccagctgg atgacggctc caggttgcag
      661 gacgctttct gttcccgaca gactctctgg gagcttctca gccattttgc acagaccagg
      721 gagcgtctgc agcagctggg tgagaagacc cctgtctgcg tgtacatgag gaatgaggtg
      781 actggtagag ctgccttaca gaacacaacc cttcagtcac tgggcctgac aggtggtagt
      841 gccaccatca ggtttgtcat aaagcagtgt gacaccgccg gcaaacagga gtccatagcc
      901 gtcaggagca aggccccggg aagtccggtc tcctccttgt cagccgacca ggcatccagt
      961 agcacattgc ttcctctgaa ctctggggag ttcagcaggg gagacctaaa ccatgagggt
     1021 gatgcaaaca cctcagggac cggccttgag ggtggcccca aaccaacgga tgctcaaaca
     1081 aagcaaagca ccagtgagcc tgcatcggcc cccttcgttc ccttctctgg ggggggccag
     1141 cggctggggg gcccgtctgc gtccttgaga cctctaacat caccttcggc caattcatcc
     1201 aagtctttct ctggccctgg gggcccttct aagcccaaga agccaaagcc tggtgaggag
     1261 ccccagcagg agcctgaacc gcccgttgac cgagatcctg tggtgtacca ccctgaccta
     1321 gaggacttgc tacagccctg gccggcagag gtgcctgacg agttctttga ggtgactgtg
     1381 gatgatgtga ggagacgctt ggcccagctc aagagtgagc ggaagcgtct ggaagaagcc
     1441 cccctggtga ccaaggcttt cagggaggct cagatgaagg agaaactgga acgttaccca
     1501 aaggtggctc tgcgggtcct attccctgac cgctacatcc tgcagggctt cttccgcccc
     1561 agtgagacag tgggggatct tcgagacttt gttaggagcc acctggggaa ccccgagctc
     1621 tccttttacc tattcatcgc tccacccaaa atggtcctgg atgaccacac gctgactctc
     1681 tttcaggcaa acctcttccc tgctgcgctt gtgcactttg gagctgaaga accaacaggt
     1741 ctctatctgg aacctgggct gctagaacac accgtctccc catctacagc tgatgtgctg
     1801 gtggccaggt gtatgtcccg ggcttccggg tccccacctc tgctgccagc ccctgaccct
     1861 gtatccctgg agtctgagcc aatcgctgag gatggggcac tggggccccc agagcccatc
     1921 caagggacag cccagcctgt gaagagaagc ctgggcaaag tacccaagtg gctgaagctg
     1981 ccagccagca agaggtgaga gctaccagcc tggagatgcc tatcaccagc cacaggaccc
     2041 tacccccacc caccccgagc aggaataaag agtcgcgctt ctctcaaaaa aaaaaaaaaa
     2101 aaaaaaaaaa aaaaaaaaaa a
//
