LOCUS       NM_025711               2362 bp    mRNA    linear   ROD 08-OCT-2024
DEFINITION  Mus musculus asporin (Aspn), transcript variant 1, mRNA.
ACCESSION   NM_025711
VERSION     NM_025711.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Hosen,S., Ikeda-Yorifuji,I. and Yamashita,T.
  TITLE     Asporin and CD109, expressed in the injured neonatal spinal cord,
            attenuate axonal re-growth in vitro
  JOURNAL   Neurosci Lett 833, 137832 (2024)
   PUBMED   38796094
  REMARK    GeneRIF: Asporin and CD109, expressed in the injured neonatal
            spinal cord, attenuate axonal re-growth in vitro.
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2362)
  AUTHORS   Liu,S., Yu,X., Guo,Q., Zhao,S., Yan,K., Hou,M., Bai,F. and Li,S.
  TITLE     Periodontal ligament-associated protein-1 promotes
            osteoclastogenesis in mice by modulating TGF-beta1/Smad1 pathway
  JOURNAL   J Periodontol 95 (2), 146-158 (2024)
   PUBMED   37436700
  REMARK    GeneRIF: Periodontal ligament-associated protein-1 promotes
            osteoclastogenesis in mice by modulating TGF-beta1/Smad1 pathway.
REFERENCE   4  (bases 1 to 2362)
  AUTHORS   Kinoshita,M., Yamada,S., Sasaki,J., Suzuki,S., Kajikawa,T.,
            Iwayama,T., Fujihara,C., Imazato,S. and Murakami,S.
  TITLE     Mice Lacking PLAP-1/Asporin Show Alteration of Periodontal Ligament
            Structures and Acceleration of Bone Loss in Periodontitis
  JOURNAL   Int J Mol Sci 24 (21), 15989 (2023)
   PUBMED   37958972
  REMARK    GeneRIF: Mice Lacking PLAP-1/Asporin Show Alteration of Periodontal
            Ligament Structures and Acceleration of Bone Loss in Periodontitis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2362)
  AUTHORS   Zhou,G., Yan,X., Chen,Z., Zeng,X. and Wu,F.
  TITLE     ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic
            Differentiation of Bone Marrow Mesenchymal Stromal Cells and
            Extracellular Matrix Mineralization of Osteoblasts
  JOURNAL   Orthop Surg 15 (9), 2423-2434 (2023)
   PUBMED   37427673
  REMARK    GeneRIF: ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic
            Differentiation of Bone Marrow Mesenchymal Stromal Cells and
            Extracellular Matrix Mineralization of Osteoblasts.
REFERENCE   6  (bases 1 to 2362)
  AUTHORS   Kou,I., Nakajima,M. and Ikegawa,S.
  TITLE     Expression and regulation of the osteoarthritis-associated protein
            asporin
  JOURNAL   J Biol Chem 282 (44), 32193-32199 (2007)
   PUBMED   17804408
  REMARK    GeneRIF: The findings indicate that TGF-beta1 induces ASPN through
            Smad3 but that this induction is indirect.
REFERENCE   7  (bases 1 to 2362)
  AUTHORS   Yamada,S., Tomoeda,M., Ozawa,Y., Yoneda,S., Terashima,Y.,
            Ikezawa,K., Ikegawa,S., Saito,M., Toyosawa,S. and Murakami,S.
  TITLE     PLAP-1/asporin, a novel negative regulator of periodontal ligament
            mineralization
  JOURNAL   J Biol Chem 282 (32), 23070-23080 (2007)
   PUBMED   17522060
  REMARK    GeneRIF: PLAP-1/asporin plays a specific role in the periodontal
            ligament as a negative regulator of cytodifferentiation and
            mineralization by regulating BMP-2 activity to prevent the
            periodontal ligament from developing non-physiological
            mineralization
REFERENCE   8  (bases 1 to 2362)
  AUTHORS   Kizawa,H., Kou,I., Iida,A., Sudo,A., Miyamoto,Y., Fukuda,A.,
            Mabuchi,A., Kotani,A., Kawakami,A., Yamamoto,S., Uchida,A.,
            Nakamura,K., Notoya,K., Nakamura,Y. and Ikegawa,S.
  TITLE     An aspartic acid repeat polymorphism in asporin inhibits
            chondrogenesis and increases susceptibility to osteoarthritis
  JOURNAL   Nat Genet 37 (2), 138-144 (2005)
   PUBMED   15640800
REFERENCE   9  (bases 1 to 2362)
  AUTHORS   Henry,S.P., Takanosu,M., Boyd,T.C., Mayne,P.M., Eberspaecher,H.,
            Zhou,W., de Crombrugghe,B., Hook,M. and Mayne,R.
  TITLE     Expression pattern and gene characterization of asporin. a newly
            discovered member of the leucine-rich repeat protein family
  JOURNAL   J Biol Chem 276 (15), 12212-12221 (2001)
   PUBMED   11152695
REFERENCE   10 (bases 1 to 2362)
  AUTHORS   Lorenzo,P., Aspberg,A., Onnerfjord,P., Bayliss,M.T., Neame,P.J. and
            Heinegard,D.
  TITLE     Identification and characterization of asporin. a novel member of
            the leucine-rich repeat protein family closely related to decorin
            and biglycan
  JOURNAL   J Biol Chem 276 (15), 12201-12211 (2001)
   PUBMED   11152692
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AW106088.1 and BC034888.1.
            
            On Feb 19, 2010 this sequence version replaced NM_025711.2.
            
            Summary: This gene encodes a member of the small leucine-rich
            proteoglycan family. The encoded protein is an extracellular matrix
            protein that modulates the transforming growth factor-beta
            signaling pathway, regulating cartilage matrix gene expression and
            cartilage formation. The protein plays a role in the pathology of
            osteoarthritis. Alternative splicing results in multiple transcript
            variants.[provided by RefSeq, Feb 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034888.1, AK014504.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-98                AW106088.1         1-98
            99-2362             BC034888.1         1-2264
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 25.31 cM"
     gene            1..2362
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /note="asporin"
                     /db_xref="GeneID:66695"
                     /db_xref="MGI:MGI:1913945"
     exon            1..275
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /inference="alignment:Splign:2.1.0"
     exon            276..551
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /inference="alignment:Splign:2.1.0"
     exon            552..664
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /inference="alignment:Splign:2.1.0"
     exon            665..878
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /inference="alignment:Splign:2.1.0"
     exon            879..989
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1083
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1222
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..2354
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2330..2335
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /note="hexamer: ATTAAA"
     polyA_site      2354
                     /gene="Aspn"
                     /gene_synonym="4631401G09Rik; Plap1; Slrr1c"
                     /note="major polyA site"
ORIGIN      
        1 gtgatggaaa acatatgagc agtaacaagt tttaatctcg ctcttcagta ctaacatgga
       61 ctaatctgtg gaagcagttt attccagtat cacccaggag cagccacaca gaggctggta
      121 ggagggctgg atttttgttc tctttttttc ttttctttaa atgtaacact tctttatttt
      181 ttcttcttga agagtcttga ggatacttac attgcagtta agtagtacag ggtggataaa
      241 ttctactttg aagaaaactt ctctcctctg acaaggttgg acttgtacac aggccagcat
      301 gaaggagtat gtgatgctac tgcttttggc tgtgtgctct gccaaaccct tctttagccc
      361 ttcccacaca gcactgaaga atatgatgtt gaaggatatg gaagacacag atgatgacga
      421 taacgatgat gacgacaact ctctttttcc aacgaaagag ccagtgaacc cttttttccc
      481 tttcgatttg tttccaacat gtccatttgg gtgccaatgt tactctcgag ttgttcactg
      541 ctctgatcta ggtctgacat cggttccaaa caacattcca tttgatactc gaatggttga
      601 ccttcaaaat aataaaatca aggaaattaa agaaaatgac tttaaaggac tcacttcact
      661 ttatgctctg attctgaaca acaacaagct aacaaagatt cacccaaaaa cctttctaac
      721 cacaaagaaa ttgagaaggc tatatttatc ccacaaccaa ctaagtgaaa ttccacttaa
      781 tcttcccaaa tcattagcag aactcagaat tcatgataat aaagttaaga agatacaaaa
      841 ggacacgttc aagggaatga atgctttaca tgttttggaa atgagtgcaa accctcttga
      901 gaacaacggg atagaaccag gggcatttga aggggtgaca gtattccata tcaggatcgc
      961 tgaagcaaaa ctaacctcaa ttccaaaagg cctaccacca actttgctgg agcttcattt
     1021 agattttaat aaaatttcaa cggtggaact tgaagatctt aaacggtaca gggaactgca
     1081 aaggctgggt cttggaaaca acagaatcac agatattgaa aatggaactt ttgctaatat
     1141 accacgtgtg agagagatac acttggaaca caataaacta aaaaaaatcc cttcaggatt
     1201 acaggagttg aaatacctcc agataatctt ccttcattat aattcaattg caaaagtggg
     1261 agtgaatgac ttctgtccaa cagtgccaaa gatgaagaaa tctttataca gtgcaataag
     1321 tttattcaac aacccaatga agtactggga aatacaacct gcaacatttc gttgtgttct
     1381 tggcagaatg agtgttcagc ttgggaatgt tggaaaataa ttcatgacat ccattaaata
     1441 taaaattcaa aaatgtatac atttggaata cttgaactgt cctagtaatg gtagtattat
     1501 acacataagc aaaattctat tctatatggt caatgacaaa aaatttcaac agaattttgc
     1561 ctaattattg atgctcagaa taaatttcta ttgcagtgtc cttctgcaca tgaatgattc
     1621 ttgcgtaaat cttttgcttg aacattcttt tttcggcaaa aaaagatatt tagtatttaa
     1681 cccttcatta tcaagtcagt caaacagaat tgtactgtaa acagaatgct tgacttagta
     1741 acatttgtgt catatctttg ctgttagaaa aacaaaactg gcaagaacag cattttgaag
     1801 agtacatata tttttagtag ttttttaaaa aaaacttgga cagtactgta atgtttccaa
     1861 taatgttgga atacatatag tttgacagaa tcaaaattct caactcataa taaagcttca
     1921 agtattcaca gataatattc atcagagttg gtttgggcta taacacatga atatcttttt
     1981 taaattatta actggctata aaattgtaaa aatataatga ctgctaatat aaaatctata
     2041 atgtgcattt tatgatcagt tatataagct ttgaagaaca cagtaactgt taggttacat
     2101 agtgttatta cttcaactag gaatatttca ggatatccct ttggaacagt atggacgcca
     2161 atcaatttta tatcaactta tctcttcaaa tatgcacatt gggtaatgcc tggaaacata
     2221 gctaaggtga caaaaactga aaactgaaca aaacttaata gtactttcat gtgttttttt
     2281 taaactgata ttcattatga attaagtaaa aagtgacaat aaggaaaaca ttaaatactg
     2341 gttttcaata aaaaaaaaaa aa
//
