LOCUS       NM_009791               9866 bp    mRNA    linear   ROD 16-OCT-2024
DEFINITION  Mus musculus abnormal spindle microtubule assembly (Aspm), mRNA.
ACCESSION   NM_009791
VERSION     NM_009791.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Barao,S., Xu,Y., Llongueras,J.P., Vistein,R., Goff,L.,
            Nielsen,K.J., Bae,B.I., Smith,R.S., Walsh,C.A., Stein-O'Brien,G.
            and Muller,U.
  TITLE     Conserved transcriptional regulation by BRN1 and BRN2 in
            neocortical progenitors drives mammalian neural specification and
            neocortical expansion
  JOURNAL   Nat Commun 15 (1), 8043 (2024)
   PUBMED   39271675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 9866)
  AUTHORS   Yamanaka,T., Kurosawa,M., Yoshida,A., Shimogori,T., Hiyama,A.,
            Maity,S.N., Hattori,N., Matsui,H. and Nukina,N.
  TITLE     The transcription factor NF-YA is crucial for neural progenitor
            maintenance during brain development
  JOURNAL   J Biol Chem 300 (2), 105629 (2024)
   PUBMED   38199563
REFERENCE   4  (bases 1 to 9866)
  AUTHORS   Tonosaki,M., Fujimori,A., Yaoi,T. and Itoh,K.
  TITLE     Loss of Aspm causes increased apoptosis of developing neural cells
            during mouse cerebral corticogenesis
  JOURNAL   PLoS One 18 (11), e0294893 (2023)
   PUBMED   38019816
  REMARK    GeneRIF: Loss of Aspm causes increased apoptosis of developing
            neural cells during mouse cerebral corticogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9866)
  AUTHORS   Razuvaeva,A.V., Graziadio,L., Palumbo,V., Pavlova,G.A.,
            Popova,J.V., Pindyurin,A.V., Bonaccorsi,S., Somma,M.P. and Gatti,M.
  TITLE     The Multiple Mitotic Roles of the ASPM Orthologous Proteins:
            Insight into the Etiology of ASPM-Dependent Microcephaly
  JOURNAL   Cells 12 (6), 922 (2023)
   PUBMED   36980263
  REMARK    GeneRIF: The Multiple Mitotic Roles of the ASPM Orthologous
            Proteins: Insight into the Etiology of ASPM-Dependent Microcephaly.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9866)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 9866)
  AUTHORS   Bond,J., Roberts,E., Mochida,G.H., Hampshire,D.J., Scott,S.,
            Askham,J.M., Springell,K., Mahadevan,M., Crow,Y.J., Markham,A.F.,
            Walsh,C.A. and Woods,C.G.
  TITLE     ASPM is a major determinant of cerebral cortical size
  JOURNAL   Nat Genet 32 (2), 316-320 (2002)
   PUBMED   12355089
REFERENCE   8  (bases 1 to 9866)
  AUTHORS   Luers,G.H., Michels,M., Schwaab,U. and Franz,T.
  TITLE     Murine calmodulin binding protein 1 (Calmbp1): tissue-specific
            expression during development and in adult tissues
  JOURNAL   Mech Dev 118 (1-2), 229-232 (2002)
   PUBMED   12351193
  REMARK    GeneRIF: tissue distribution of Calmbp1 in fetal and adult mice
REFERENCE   9  (bases 1 to 9866)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
REFERENCE   10 (bases 1 to 9866)
  AUTHORS   Craig,R. and Norbury,C.
  TITLE     The novel murine calmodulin-binding protein Sha1 disrupts mitotic
            spindle and replication checkpoint functions in fission yeast
  JOURNAL   J Cell Sci 111 (Pt 24), 3609-3619 (1998)
   PUBMED   9819352
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK052326.1, CN664812.1, CV556405.1, BI989704.1, AC158946.4,
            AY971958.1 and DV662096.1.
            
            On Mar 21, 2006 this sequence version replaced NM_009791.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF533752.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2999              AK052326.1         2-3000
            3000-3347           CN664812.1         154-501
            3348-4058           CV556405.1         61-771
            4059-4602           BI989704.1         57-600
            4603-7923           AC158946.4         94230-97550
            7924-9528           AY971958.1         3970-5574
            9529-9866           DV662096.1         135-472
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 61.45 cM"
     gene            1..9866
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /note="abnormal spindle microtubule assembly"
                     /db_xref="GeneID:12316"
                     /db_xref="MGI:MGI:1334448"
     exon            1..432
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..9528
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /note="calmodulin-binding protein 1; calmodulin-binding
                     protein Sha1; spindle and hydroxyurea checkpoint abnormal
                     protein; asp (abnormal spindle)-like, microcephaly
                     associated"
                     /codon_start=1
                     /product="abnormal spindle-like microcephaly-associated
                     protein homolog"
                     /protein_id="NP_033921.3"
                     /db_xref="CCDS:CCDS35729.1"
                     /db_xref="GeneID:12316"
                     /db_xref="MGI:MGI:1334448"
                     /translation="MATMQAASCPEERGRRARPDPEAGDPSPPVLLLSHFCGVPFLCF
                     GDVRVGTSRTRSLVLHNPHEEPLQVELSLLRAAGQGFSVAPNRCELKPKEKLTISVTW
                     TPLREGGVREIVTFLVNDFLKHQAILLGNAEEPKKKKRSLWNTSKKIPASSKHTKRTS
                     KNQHFNESFTISQKDRIRSPLQPCENLAMSECSSPTENKVPTPSISPIRECQSETCLP
                     LFLRESTAYSSLHESENTQNLKVQDASISQTFDFNEEVANETFINPISVCHQSEGDRK
                     LTLAPNCSSPLNSTQTQIHFLSPDSFVNNRYTSDNDLKSMKNVLSDTFRKDPAESVCL
                     ESQTVHEVCQTILSPDSFLNDNYGLKKGLNFKSVNPVLSPTQFVKDSMGHVGQQTGKS
                     NEASQDWRINEGLAYTPECQHAQTPSSRSEKQNPVEVKPHTYDFTKQKPKISEFQDAF
                     CHQSKQPHKRRPILSATVTKRKPTNAREKLPEINKPDAKRCLEGLVGERGKEVGSLRE
                     KGFHSSLPVVEPGVSKALSYRDEVTPATVVVARKRKSHGTVGDANGKVAAEEWMDMCE
                     VKRIHFSPLESTPSTVARTTKKEGHTSKRISSLERSGLKKKMDSSILKTPLSKTKKKR
                     RSIVAVAQSHLTFIKPLKAAIPRHPMPFAAKNMFYDERWKEKQEQGFTWWLNYILTPD
                     DFTVKTNVSKVNAASLVLGAESQHKISVPKAPTKEEVSLRAYTASCRLNRLRRTACSL
                     FTSEKMVKAIKKVEIEIEVGRLLVRKDRHLWKDIGQRQKVLNWLLSYNPLWLRIGLET
                     VFGELIPLADNSDVTGLAMFILNRLLWNPDIAAEYRHPTVPLLFRDGHEAALSKFTLK
                     KLLLLICFLDHAKISRLIDHDPCLFCKDAEFKASKELLLAFSRDFLSGEGDLSRHLSF
                     LGLPVSHVQTPLDEFDFAVTNLAVDLQCGVRLVRTVELLTQNWNLSDKLRIPAISRVQ
                     KMHNVDLVLQVLKSRGVPLTDEHGSAISSKDVVDRHREKTLGLLWKIALAFQVDISLN
                     LDQLKEEIDFLKHTHSIKRAMSALTCPSQAITNKQRDKRISGNFERYGDSVQLLMDWV
                     NAVCAFYNKKVENFTVSFSDGRILCYLIHHYHPCYVPFDAICQRTSQSVACAQTGSVV
                     LNSSSESEGGCLDLSLEALDHESTPEMYKELLENEKKNFHLVRSAARDLGGIPAMIHH
                     SDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKRDLKHHQERDK
                     AARVIQSVVLNFLSRRRLQKNVSAALVIQKCWRRVSAQRKLRMLKNEKLAKLQNKSAV
                     LIQAYWRRYSTRKRFLRLKHYSVILQSRIRMKIALTSYKRYLWATVTIQRHWRAYLSR
                     KRDQQIFRKLKSSSLVIQFMFRRWKRRKLQLQTKAAVTLQRAFREWHLRKQIRERSAV
                     VIQSWYRMHRELQKYIYIRSCVIVIQRRVRCFQAQKLYKRRKDAILTLQKHYRARQKG
                     KLAHADYLQKRAATIRLQAAFRGMKARHSYRLQIGAACVLQSYWRMRQERVRFLNLKK
                     MVIKLQAHIRKYQQLQKYKKIKKAAITIQTHFRASISARRVLASYQKTRSSVIVLQSA
                     CRGMQARKAFRHALASVIKIQSYYRAYICRKTFQNFKNATIKLQSIVKMKQSRKQYLQ
                     IRAAALFIQRWYRSQKLASQKRKEYIQVRESCIKLQSHFRGCLVRKQLRLQCKAAISL
                     QSYFRMRTARQRYLKMCKAALVIQSFYCAYRAQISQRKNFLQVKRAAICLQAAYRGCK
                     VRRQIKQQSTAAVTIQRVFRGHSQRMKYQTMLQSAVKIQRWYRAQKVAYDMRIQFLKT
                     REAVVCLQSAYRGWQVRQQLRRQHEAAVKIQSTFRMAVAQQQYKLLRAAAAVIQQHVR
                     ARAAGKRQHLAYIQLRHAALVFQAAWKGKMLRRQIARQHQCAALIQSYYRMHIQRRKW
                     SIMKTAALQIQLCYRAYKVGKEQRHLYLKTKAAVVTLQSAYRGMKVRKRVAECHKAAV
                     TIQSKFRAYRTQKKYTTYRTSAIVIQRWYRNIKITTQQHQEYLNLRRAAVQVQAAYRG
                     IRVRRRIQHMHMAATLIEAMFKMRQSRVRYLKMRTAALIIQVRYRAYYLGKIQHEKYL
                     RTLKAIKTLQAGVRGARVRRTVRKMHFAATLIQSHFRGHRQQTYFHRLRKAATMVQQR
                     YRAVKEGSAEFQRYSRLRRSVLLIQAAFRGLRTRRHLKAMHLAATLIQRRFRTFAMRR
                     KFLSLRKTAIWIQRQYRARLYAKYSRQQLLLEKAVIKIQSSYRGWVVRKRVQKMHRAA
                     TVIQATFRMHGAYMRYQHLKRASVVIQVHTAAELQRQKHAAVILQAAVRGMKTRSHLK
                     TMHSSATLIQSQFRAFIVRRRFIALRKAAIFVQRKFRATLYAKHKLHQFLQLRKAAIT
                     IQSSYRRLMVQKKLQEMHRAAALIQATFRMHRTYVAFHIWKCASIRIQQCYRTYRTIK
                     LQKEKLIREEQHSAAVLIQSTYRMYRQRCFYQQRRWAAKVIQKTYRANKRRQDLLYVC
                     KEETPLLQMHFQGLNTAKQGRQQHGAAMITQKHFRAFKARRLMEAERGFQAGCRKYKA
                     KKYLSKVEAACRIQAWYRRWRAHKKYLTLLKAVNIIEGYLSAQLARRRFLKMRAAAII
                     IQRKWRATLSVRGARENLKRHRAACVIQAHFRGYQARQSFLQQRSAVLIIQRHVRAMV
                     AAKQERIKYIKLKKSTVVVQALVRGWLVRKRVSEQKAKTRLFHFTAAAYCHMCALKIQ
                     RAYRLHVTLRNAKKHMDSVIFIQRWFRKRLQRKRFIEQYHKILSTRREAHACWLQQDR
                     AASVIQKAVRRFLLCRRQEKITSCATRIQALWRGYSWRKKNDHTEIKAIRRSLRAVST
                     TVEEENKLYRRTERALHHLLTYKHLSAILDALKHLEVVTRLSPLCCENMAESGAVSTI
                     FVVIRSCNRSVPCMEVVGYAVQVLLNVAKYDKTIAAVYEAENCVDTLLELLQVYREKP
                     GDRVAEKSASIFTRTCCLLAVLLKTEQCAFDAQSRSKVTDRIYRLYKFTVPKHKVNTQ
                     GLFDKQKQNSCVGFPCIPERTMKTRLVSRLKPQWVLRRDNVEEITNSLQAIQLVMDTL
                     GISY"
     exon            433..576
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..1984
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2089
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2236
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2482
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            2483..2550
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..2692
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2823
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            2824..2999
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            3000..3145
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            3146..3231
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            3232..3453
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3661
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            3662..3804
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            3805..3933
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            3934..4128
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            4129..7923
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            7924..8090
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            8091..8178
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            8179..8388
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            8389..8538
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            8539..8730
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            8731..8923
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            8924..9078
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            9079..9255
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            9256..9425
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     exon            9426..9866
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9637..9642
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
                     /note="hexamer: AATAAA"
     polyA_site      9661
                     /gene="Aspm"
                     /gene_synonym="Calmbp1; D330028K02Rik; MCPH5; Sha1"
ORIGIN      
        1 gcgatatttg aaccgagcaa aagcggctgg gctactttcc cgggagttta ggggatcttt
       61 ttggcttttt gatctttgtc cttgtcctgg tcgttggctt ccgggaagcc ggcgggagcc
      121 ggacagcggg tacctgactg cccggtacgg gtggctgcca tggcgacgat gcaggcagcc
      181 tcctgcccag aggagagggg gcggcgggcg cgaccagatc ctgaggccgg ggacccgtct
      241 ccgccggtgc tgttgctcag ccacttctgc ggcgttccct tcctctgctt cggggatgtc
      301 cgcgtgggca cgtcgcggac gcggtctctg gtcctgcaca acccgcacga ggaacctctg
      361 caggtggagc tgtcgctgct gcgcgccgca ggccagggct tcagcgtggc gccgaaccgc
      421 tgcgagctga agcctaaaga aaaacttacc atttccgtta cctggacgcc actgcgagaa
      481 gggggagtga gggagattgt cacatttctt gtaaatgatt tcctgaagca ccaggctata
      541 ttactaggaa atgcagaaga gcctaagaag aaaaagagaa gtctttggaa taccagtaag
      601 aagattccag cctcttcaaa acatacaaaa aggacttcca aaaaccaaca ttttaatgaa
      661 tcatttacta tttcacaaaa agacagaatt aggagcccac tgcagccttg tgaaaatctg
      721 gctatgagtg aatgctcttc cccaacagaa aacaaagtcc ccaccccatc cattagtcct
      781 attagagaat gccagagtga aacttgcttg ccactgtttt tacgcgagtc cactgcctat
      841 tcatctcttc atgaatctga aaatacacaa aatttaaaag tacaagatgc cagcatttca
      901 caaacttttg attttaatga ggaagtcgca aatgaaactt ttattaatcc cattagtgtc
      961 tgtcaccaga gtgaagggga taggaaactc acgcttgccc caaactgttc ttcacctttg
     1021 aatagtacac agacccaaat acactttcta agtccagatt cttttgtaaa caacaggtat
     1081 acgtctgata atgacctgaa atcaatgaaa aatgttttat cagacacatt taggaaagat
     1141 cccgcagagt ctgtatgtct ggaatcccaa actgtccatg aagtttgcca aacaatctta
     1201 agcccagatt cttttttaaa tgataattat ggacttaaaa aagggctaaa cttcaagtcc
     1261 gtcaatcccg ttttgtcgcc aacacaattt gtaaaggata gcatgggaca cgtaggtcag
     1321 caaacgggta agtcaaatga agcttctcag gattggagaa taaatgaagg gttggcgtat
     1381 acccccgagt gtcagcatgc acagactccc agcagcagat ctgaaaaaca aaaccctgta
     1441 gaagtgaagc cacatacgta tgattttaca aagcaaaagc ccaagatttc tgagtttcag
     1501 gatgcttttt gtcaccagag taaacaaccg cacaagagac gcccaattct ttctgccact
     1561 gttacgaaaa ggaaacccac caatgccaga gaaaagctac ctgagattaa caagccagac
     1621 gcaaaaagat gtctcgaagg tctagtaggt gaacgtggaa aagaagtggg tagcctgaga
     1681 gagaaaggtt ttcattcttc tcttcccgtt gtggagccag gagtgagtaa agctctgagc
     1741 tacagggatg aagtaacgcc tgcaactgtt gtcgttgctc ggaaaagaaa gagccacgga
     1801 acagtgggag atgcaaatgg aaaagtagca gctgaagaat ggatggacat gtgtgaagtc
     1861 aaacgaatcc atttttctcc tctggaatct acaccctcaa ctgttgcaag aaccacaaag
     1921 aaagaagggc atacctcaaa gcggataagc agcctggagc ggtcaggcct gaagaagaaa
     1981 atggattcat caatattaaa aacacctctt tctaagacaa agaaaaagag aagatccatt
     2041 gttgctgtag cacagtctca tttgaccttt ataaagccat taaaagcagc tattcctaga
     2101 cacccaatgc cgtttgctgc aaagaacatg ttctatgacg aacgctggaa ggaaaagcag
     2161 gaacagggct tcacttggtg gctaaattat atattaacac ctgatgactt cactgtaaaa
     2221 accaatgttt ctaaagtgaa cgctgccagc cttgttttgg gagcggaaag ccaacataaa
     2281 ataagtgtcc cgaaagcacc tactaaagag gaagtgtctc tcagagctta tacagccagc
     2341 tgcaggctga atagattacg ccgtacagct tgctccttgt ttacttctga aaaaatggtt
     2401 aaagctatta aaaaggttga aatcgaaatt gaagttgggc ggctacttgt tcgaaaagac
     2461 cgacacctct ggaaagatat tggacaacgc caaaaagtcc tgaattggct cttgtcatat
     2521 aatcctctgt ggcttcgaat tggtctggag acagtttttg gagaactgat ccctctggca
     2581 gacaacagtg acgtcacggg attggcgatg ttcattctga atcgcttact ttggaatcct
     2641 gacatagcag ctgaatatag acaccccact gttcctctcc tgtttcggga tggtcatgaa
     2701 gcagccttgt ctaagttcac attgaaaaaa ttgctgttgt tgatctgttt ccttgatcat
     2761 gctaaaattt ccagacttat tgatcatgat ccttgtctct tctgtaaaga tgctgaattc
     2821 aaggctagca aagagcttct tctagccttt tcacgggatt tcctcagtgg tgaaggtgac
     2881 ctttctcgtc accttagctt cttggggtta cctgtgagcc acgttcagac accacttgat
     2941 gaatttgatt ttgctgttac aaatcttgca gtggacttgc agtgtggggt tcgccttgtg
     3001 cgaactgtgg agcttctcac acagaactgg aatctgtccg acaaactcag gatccctgca
     3061 ataagtcgag ttcaaaagat gcacaatgtt gatctcgtcc ttcaggttct caagtctcgg
     3121 ggagttccac tgactgacga gcatgggagc gctatctcgt ctaaggatgt tgtggacagg
     3181 cacagagaga aaacccttgg gttgctttgg aaaatagcac tggctttcca ggtggatatt
     3241 tcccttaatt tagatcaatt aaaagaagaa attgattttc taaagcacac acacagtata
     3301 aagagagcta tgtctgcact aacatgccct tcacaagcta ttactaataa gcaaagagac
     3361 aaaaggatta gtgggaactt tgaacgctat ggtgacagtg tgcagttgtt gatggattgg
     3421 gtaaatgcag tttgtgcctt ctataataaa aaggtggaga attttacagt gtctttctca
     3481 gatggccgca tactatgcta tttgatccac cattaccacc cgtgctatgt gcctttcgat
     3541 gctatatgcc agcgcaccag tcagtccgtg gcctgcgcac agactggttc agtggtattg
     3601 aattcttcat cggaatctga aggaggttgt ctggatctgt ctcttgaagc gcttgatcac
     3661 gaaagtactc cagaaatgta caaagagctt ctagaaaacg agaagaaaaa tttccatttg
     3721 gtgcgatctg cagctaggga tctgggagga atacctgcta tgattcatca ttcagacatg
     3781 tcaaatacaa ttccagacga aaaggtggtt atcacctatt tgtcatttct ttgtgcaagg
     3841 cttttggatc tccgtaaaga aataagagct gctcgactta tacagacaac atggagaaaa
     3901 tataaactaa aaagagatct gaaacaccat caggaaagag acaaagctgc tagagttatt
     3961 cagtcagttg ttctcaattt tctaagtaga cgaagattgc aaaaaaacgt cagtgcagct
     4021 ctggtcattc agaaatgctg gcgaagagtc tcagcgcaaa ggaagttgcg gatgctgaaa
     4081 aatgaaaaac tggcaaaact tcaaaataaa tcagcagtgc tcattcaggc ttattggaga
     4141 agatactcca ccagaaaacg gtttctgagg ttgaagcact attcagtcat ccttcagtct
     4201 aggataagaa tgaaaattgc tcttacatct tacaaacgat atctttgggc cacagttaca
     4261 attcagaggc attggcgtgc ctatttaagt agaaaacgag atcagcaaat atttaggaag
     4321 ctgaaatcct catcccttgt aatccagttt atgttcagaa ggtggaagcg acgcaagctg
     4381 cagttacaaa ccaaagctgc agtaacattg cagcgagctt ttagagaatg gcatctcagg
     4441 aaacaaatac gagaaagatc cgctgttgtg atacagtcat ggtacagaat gcacagggaa
     4501 ttgcagaaat acatttatat taggtcttgt gttattgtca tccagagaag agttcggtgc
     4561 tttcaagccc aaaagttata taagagaagg aaagatgcca tcctgactct ccagaaacat
     4621 tacagggcac gtcagaaggg gaagctagca cacgctgact atttgcagaa acgagctgcc
     4681 accattcgac tgcaggctgc attcaggggg atgaaggctc gccattcata caggttgcag
     4741 attggagctg cctgtgttct tcagtcctat tggagaatga gacaggaaag agttagattt
     4801 ttaaacctta aaaagatggt tattaagttg caagcacaca taagaaaata ccaacaacta
     4861 cagaaatata agaagataaa gaaagcagcc attacaattc agactcattt ccgagcttcc
     4921 atttcagcca ggagggttct agcatcttat cagaaaacaa ggtcttcagt cattgttcta
     4981 cagtctgcat gtagagggat gcaagccaga aaagcatttc gccatgcttt agcatccgtt
     5041 ataaagattc agtcatatta tcgggcttat atatgtagga agacatttca gaacttcaaa
     5101 aatgccacaa taaagctgca gtcaattgtc aaaatgaaac aatcacggaa gcagtattta
     5161 caaataagag cagctgcact gtttatccag cggtggtacc gttcccaaaa actggcctca
     5221 caaaagagaa aagagtacat acaggtgcgt gaatcctgta tcaaactgca gtcacacttt
     5281 agaggatgcc tggtcagaaa gcagttgagg ttgcaatgta aagctgccat ttctctacag
     5341 tcttatttca gaatgagaac agcgaggcag cgctacctga agatgtgcaa ggcggctctt
     5401 gtcattcaga gtttctactg tgcctacaga gcacaaatca gtcaaaggaa gaacttcttg
     5461 caggtcaaaa gagcagctat ttgtttgcag gcagcttaca gaggctgcaa agtacgccgg
     5521 caaatcaagc agcagtctac ggctgctgtt acaattcaga gggtttttag aggccacagt
     5581 cagaggatga aataccagac tatgcttcag tctgctgtga agattcagag atggtacagg
     5641 gcacagaagg ttgcttatga catgagaatt caatttctaa agactagaga ggctgtggtt
     5701 tgcctccagt ctgcctaccg tggctggcag gttcggcaac agctcaggag gcaacatgag
     5761 gctgcagtga agatccagtc tacgtttaga atggccgtgg cccagcaaca gtataaacta
     5821 ctaagagcag ctgcagcagt catccagcag catgtgaggg cacgggctgc agggaagagg
     5881 cagcacttgg cgtacatcca gctccgtcat gcagctctgg tgtttcaggc tgcatggaag
     5941 ggaaagatgc tgagaagaca gattgcccgg cagcatcaat gcgctgccct catacagtca
     6001 tattacagga tgcacattca gcgcaggaag tggagcatca tgaaaaccgc tgcccttcag
     6061 attcagctgt gctatagggc ttacaaagtt ggaaaagaac agcgccactt atacttgaaa
     6121 acaaaagcag ctgtagtaac tttacagtca gcttaccgtg gtatgaaagt gagaaagcgg
     6181 gtagctgaat gccacaaagc agcggtcact atacagtcta aatttagagc ctacagaaca
     6241 caaaagaagt acacgaccta tagaacttca gctattgtaa ttcagaggtg gtaccggaat
     6301 attaaaatta caacccagca acatcaggaa taccttaact taaggagggc agcagttcaa
     6361 gtccaggctg cgtatagagg cattagagtg aggagacgca ttcaacacat gcacatggcg
     6421 gccacactta ttgaagccat gtttaagatg cgtcagtcaa gagtaaggta cctcaagatg
     6481 agaacagcag ctcttataat tcaggtgaga tacagagcgt attatctagg taagattcag
     6541 catgagaagt acctgaggac tttgaaggct attaaaaccc ttcaagctgg agtcagagga
     6601 gcaagagtga ggcggactgt aagaaagatg catttcgcag caacactcat tcagtcacat
     6661 tttaggggac acaggcagca aacttacttt catcggttga ggaaggcggc aaccatggtg
     6721 cagcagagat accgagcagt gaaagaagga agcgctgagt ttcagaggta cagcagacta
     6781 aggcgttctg tactcctcat ccaggctgcc ttcaggggac tgagaactag gagacactta
     6841 aaagctatgc acctggctgc tactctcatt cagaggagat tcaggacttt cgcgatgaga
     6901 aggaagttcc tttccctcag gaaaactgcc atttggattc agagacagta ccgtgcaaga
     6961 ctttatgcca agtattcccg acaacagtta ctgttggaaa aggcagttat aaagatccag
     7021 tcatcataca gaggatgggt ggtaaggaaa agggtgcaaa agatgcacag ggcggctact
     7081 gtgattcagg ccactttccg gatgcacgga gcctatatga ggtaccagca tttgaagcgg
     7141 gcctcagttg tgatccaagt acacacagct gcagaactgc agaggcaaaa gcatgctgct
     7201 gtaatccttc aggctgccgt tagggggatg aaaaccagaa gtcatctgaa gactatgcat
     7261 tcttctgcaa cccttattca gagtcaattt agagctttta tagtaaggag acgattcatt
     7321 gctctcagaa aagctgctat atttgtgcag aggaaattcc gagccaccct ttatgccaag
     7381 cataaattgc accaattctt gcagttacga aaggcagcca ttaccataca gtcctcttat
     7441 cgaagactga tggtacaaaa gaagttacaa gaaatgcaca gggctgcagc tctcatccag
     7501 gctactttta gaatgcacag aacttacgtt gcatttcaca tttggaaatg tgcctcgatt
     7561 agaattcagc agtgttatcg aacatacaga actataaaat tacaaaaaga aaaacttatc
     7621 agagaagaac aacacagtgc tgctgtccta atacaaagca cgtacaggat gtacaggcag
     7681 cgttgcttct atcaacagcg tcggtgggca gcaaaggtaa tacaaaaaac atacagagca
     7741 aataaaagac ggcaagatct gctctatgtg tgtaaggaag aaacacctct tcttcagatg
     7801 cattttcagg gtttaaacac tgcaaaacaa ggtcggcagc agcatggggc cgctatgatt
     7861 acacagaagc attttagagc ctttaaagca aggaggctga tggaggccga gagaggcttc
     7921 caggctggat gtagaaaata taaagctaag aaatacttat ccaaagtgga agctgcctgc
     7981 aggattcaag cttggtatag gcgttggaga gcgcacaaga aatatctaac tttattgaaa
     8041 gctgttaaca ttattgaagg ttacctctct gcacagctgg cgagaaggag gttcttgaag
     8101 atgagggcgg cagctattat aattcaaagg aaatggagag ctacactttc tgtaagagga
     8161 gctcgtgaga atttaaaaag acatcgagct gcttgtgtga ttcaagccca ctttaggggc
     8221 tatcaggcaa ggcagtcctt cctgcagcag agatctgctg ttttaatcat acagagacat
     8281 gtacgagcca tggtggctgc gaagcaggaa aggataaagt atatcaaatt gaaaaagtct
     8341 acggttgttg tacaagcctt ggtgcgtggc tggttagtaa ggaaaagggt ttcagagcag
     8401 aaagccaaaa ctcggctttt ccactttaca gcagctgcct actgtcacat gtgtgctctt
     8461 aaaatccaac gagcttatcg gctccacgtg accttgagga acgctaagaa gcacatggat
     8521 tcagtcatct tcattcagag atggtttcga aaaagattac aacgaaagag gttcattgaa
     8581 cagtaccaca agatcctaag cactcgacgt gaagctcatg catgttggct ccagcaggac
     8641 agggctgcat ccgtgataca gaaggcggta cgccgctttc tcctctgcag aagacaggaa
     8701 aagatcacta gctgcgccac tagaattcag gcattatgga gaggctattc ttggaggaag
     8761 aaaaatgacc acacagaaat caaagctatc cgacgaagcc ttagagctgt gagcacgacc
     8821 gtggaggagg agaacaagct gtacagaaga actgagcgag cgcttcatca cctgctcacc
     8881 tacaaacact tgtcggccat tctggatgcg ttgaagcacc tggaggtcgt taccagactc
     8941 tctccacttt gctgtgagaa tatggctgaa agcggagcgg tatctaccat ctttgttgtg
     9001 atccgaagct gtaatcggag tgttccttgt atggaagtcg ttggatatgc tgttcaagta
     9061 ttgctgaatg tggcaaagta tgataagacg atcgcggctg tgtacgaggc cgagaactgc
     9121 gtggacacac tgctggagct tctgcaggtg taccgagaga agcctggaga ccgagtcgct
     9181 gagaagagtg ccagcatttt cacaagaact tgctgtttgt tagcagtgct gttgaagaca
     9241 gagcagtgtg cttttgatgc acaaagtaga tctaaagtca ctgatcgtat ttatcgtctc
     9301 tacaaattta cagttcctaa gcataaagta aatacacagg gattgtttga taagcagaag
     9361 cagaattcct gtgtaggctt tccttgtata ccagaaagaa ctatgaagac caggttagtt
     9421 tcaagactta agccacagtg ggttttgagg cgtgataatg tggaagaaat tacaaactcc
     9481 ctgcaagcta ttcaactggt aatggataca cttggaattt cttattagta aatggaaaca
     9541 ttttcagtgt gggacaaaaa ggaattatgc caatcatgga tacctggagc agtttttact
     9601 gtcagtgtaa actgtttaga tatgactttg tgtaaaaata aaaatgtaga tattaaagtt
     9661 actaaaactg tttaatgaat tcatttttac tatttttgaa tttttgtagt tataatttaa
     9721 ataggttttt ctctttcatt ttttttcctc caaaccctgc catatactac tacttgctct
     9781 ttttcacatt tattgctcct cttcactaat ttgtatgtat atatatgtat gtattcctaa
     9841 atacagtatg ataaatccct ataatg
//
