LOCUS       NM_001081169            2732 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus asparaginase (Aspg), mRNA.
ACCESSION   NM_001081169 XM_203596
VERSION     NM_001081169.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC112520.5.
            
            On Feb 6, 2007 this sequence version replaced XM_203596.7.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089617.1, SRR5189682.63402.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-291               AC112520.5         110657-110947       c
            292-400             AC112520.5         105330-105438       c
            401-512             AC112520.5         104538-104649       c
            513-638             AC112520.5         103125-103250       c
            639-722             AC112520.5         101980-102063       c
            723-849             AC112520.5         101103-101229       c
            850-962             AC112520.5         97564-97676         c
            963-1145            AC112520.5         96583-96765         c
            1146-1259           AC112520.5         96364-96477         c
            1260-1373           AC112520.5         95767-95880         c
            1374-1469           AC112520.5         94591-94686         c
            1470-1633           AC112520.5         94177-94340         c
            1634-1720           AC112520.5         92734-92820         c
            1721-1820           AC112520.5         91219-91318         c
            1821-2732           AC112520.5         90057-90968         c
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 61.14 cM"
     gene            1..2732
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /note="asparaginase"
                     /db_xref="GeneID:104816"
                     /db_xref="MGI:MGI:2144822"
     exon            1..291
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..1904
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.1.47"
                     /EC_number="3.5.1.1"
                     /note="60 kDa lysophospholipase [Includes: L-asparaginase;
                     asparaginase homolog; lysophospholipase-transacylase"
                     /codon_start=1
                     /product="60 kDa lysophospholipase"
                     /protein_id="NP_001074638.1"
                     /db_xref="CCDS:CCDS36567.1"
                     /db_xref="GeneID:104816"
                     /db_xref="MGI:MGI:2144822"
                     /translation="MARAMGPERRLLAIYTGGTIGMRSEGGVLVPGRGLAAVLKTLHM
                     FHDEEYAQAHSLPEDTLVLPPASPDQRIIYTVLECQPLFDSSDMTITEWVQIAQTIER
                     HYAQYQGFVVIHGTDTMAFAASVLSFMLENLQKPVVLTGAQVPIHALWSDGRENLLGA
                     LLMAGQYVIPEVCLFFQNQLFRGNRTTKVDARRFAAFCSPNLPPLATVGADVTINREL
                     VRKACGKSHLVVHSSMEPDVGLLRLYPGIPASLVRTFLQPPLKGVVMETFGSGNGPTK
                     PDLLQELRVAAEQGLIIVNCTHCLQGAVTSDYASGMAMAGAGIVSGFDMTSEAALAKL
                     SYVLGQPGLSLNDRKKLLAKDLRGEMTLPATDVLLQDGMLGCRVAWLLSMNGSQEADT
                     MKDVLLPGLALAAAHAGDLDTLQAFVELDRDLNLKDYSGQTPLHVAARRGHAAVVTML
                     LQRGADVDARNEDGQSPLLLAVRGRHQSVIGLLRAAGARLSPQELEDVGTELCRLASR
                     GDSEGLRAWWQAGADLGQPDYDGHCALQVAEAAGNADVVALLQSFKDSVCAQPQPH"
     exon            292..400
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            401..512
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            513..638
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            639..722
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            723..849
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            850..962
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1145
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1259
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1373
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1469
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1633
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1720
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1820
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..2732
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2698..2703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /note="hexamer: AATAAA"
     polyA_site      2718
                     /gene="Aspg"
                     /gene_synonym="A530050D06Rik; Gm690"
                     /note="major polyA site"
ORIGIN      
        1 tgccctttac aagagtccaa gtgtcccgct ctctactttg aaggtgactc ctcctccaca
       61 ccccgaggtc cagcctacct tggatcttca attcccacca accagaggag ccccagacct
      121 ccagtaagcc ccaccctgca gtcccctccg cttcccggtc ttgttttccc tttggctctg
      181 tccccactga ggagccagct gtctcagcca tggcgcgtgc tatgggacca gagcggcggc
      241 tgctggccat ttacactggc ggcaccatcg gcatgcggag cgaaggcgga gtgttggtac
      301 ctggcagagg cctggctgcc gtcctgaaga cacttcacat gttccacgat gaagagtatg
      361 cacaggccca cagcctgccc gaggatacgc tggtgctgcc cccggccagc cctgaccaga
      421 ggatcatcta cacggtgctg gagtgccagc cactcttcga ctccagtgac atgaccatca
      481 ctgagtgggt tcagattgcc caaaccatag agagacacta cgcacagtac cagggctttg
      541 ttgtcatcca tggcacagac accatggcct ttgctgcctc agtgctctct ttcatgctgg
      601 aaaatctgca gaaaccagtc gtcctcacgg gtgcccaggt acctatccac gcactgtgga
      661 gtgacggccg tgagaacctg ctgggggctc tgctcatggc tggccaatac gtcatccctg
      721 aggtctgcct gttcttccag aatcagctct tccggggcaa tcggacaacc aaggtggacg
      781 ctcggaggtt tgccgccttc tgctccccta acctgccccc tctagccact gtgggcgcgg
      841 atgtcacaat caaccgtgag ctggtacgga aggcctgcgg gaagagccat ctggtggtcc
      901 acagtagcat ggagcctgac gtgggcctac tgcgcctcta ccctggaatt cccgcctccc
      961 tggtccggac cttcctccag cccccactga aaggcgtggt catggagacc ttcggctctg
     1021 ggaatgggcc caccaagcca gacctactac aggagcttcg ggtggcggcc gaacaaggcc
     1081 tgatcattgt caactgtacc cattgccttc agggggctgt gacttcggac tatgcatctg
     1141 gcatggccat ggcaggagct ggcatagtct cgggcttcga catgacatca gaggctgccc
     1201 tggccaaact gtcctatgtg ctgggccagc cagggctgag cctgaatgac aggaagaagc
     1261 tgctggccaa ggatcttcgt ggggagatga cgctgcctgc aacagatgtc ttgctgcagg
     1321 atggcatgct gggctgcagg gtagcttggc ttctcagtat gaatggcagc caggaggctg
     1381 ataccatgaa ggatgttctt ctgcccggcc tggccctggc tgcagctcac gctggtgacc
     1441 tggacaccct gcaggccttt gtggaactgg acagagacct aaacctgaag gactatagtg
     1501 gtcaaacccc actgcatgtg gctgcacgga ggggccatgc cgcagtggtt accatgctgc
     1561 tgcagagggg tgcggatgtg gatgcccgta atgaggatgg tcagagccca ctattgctgg
     1621 ccgtgagggg caggcatcag agtgtcattg ggctgctgag agctgctggg gcccgcctgt
     1681 ctccccagga gctggaggac gttgggacag agctgtgcag gctggcatca aggggagaca
     1741 gcgaaggcct gcgggcatgg tggcaggctg gggctgacct gggacagcca gactatgatg
     1801 gacactgtgc cctgcaagta gctgaagcag ctggaaatgc agatgtggtg gccctgctac
     1861 agagctttaa ggacagtgtc tgtgcccagc cccagcccca ctaggtcctg ggatgggctg
     1921 ctagctcctc agtgaactct cactggtttg caactggatc ctggcacctg atatcccaga
     1981 gccaatttta gaggcagctg gaggaagagg atatgggaga cgtgtcatcc ggagcctttc
     2041 ttggggaccc ctagagaaaa cagtgttctg gaggagacag aggctgctga ggcttcacgt
     2101 ggcagaagcc catctcagat cttcacccta tccagtcttg agctgagtaa ctggaaaggt
     2161 ctaggcctgc tcccagctga cctccatccc cactgctctg gggttttgag acagggtggg
     2221 caagccccaa tctccagaga ctgcctggac tccatacaca gcgccccctg aagggggagc
     2281 cccacctctg caagtcctca gcaagccttg tttctaggaa gtgctccttg gccccaaacc
     2341 tgccatcctg ctgcagcaga aacggttcct tactgctaag ggacctcagg cacgtgccag
     2401 ccgattgacc ggctactatg ttaggacctc aggcaggcac ttccagccga ttggctacta
     2461 tgttagcaat tggctactat gttagcgatt ggctactatg ttagcgtcat tgtatttggt
     2521 gagaccttga agagtttcag aaaccagctc tgtcctggcc ttgctatctc ctccagccac
     2581 gtgttccagg agctgactca gagcggactt atcctcccta agttgagctg tcttctgctc
     2641 agctgcttcc cagcctttcc gcacgacaat ctgaaggtct ttgttgagaa gaatgtcaat
     2701 aaagttgtcg ggacagctgc tctttggcct gg
//
