LOCUS       NM_001410607            1869 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus asparagine synthetase (Asns), transcript variant 4,
            mRNA.
ACCESSION   NM_001410607
VERSION     NM_001410607.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Xu,L., Tan,C., Barr,J., Talaba,N., Verheyden,J., Chin,J.S.,
            Gaboyan,S., Kasaraneni,N., Elgamal,R.M., Gaulton,K.J., Lin,G.,
            Afshar,K., Golts,E., Meier,A., Crotty Alexander,L.E., Borok,Z.,
            Shen,Y., Chung,W.K., McCulley,D.J. and Sun,X.
  TITLE     Context-dependent roles of mitochondrial LONP1 in orchestrating the
            balance between airway progenitor versus progeny cells
  JOURNAL   Cell Stem Cell 31 (10), 1465-1483 (2024)
   PUBMED   39181129
REFERENCE   3  (bases 1 to 1869)
  AUTHORS   Zhu,Y., Ackers-Johnson,M., Shanmugam,M.K., Pakkiri,L.S., Drum,C.L.,
            Chen,Y., Kim,J., Paltzer,W.G., Mahmoud,A.I., Tan,W.L.W.,
            Lee,M.C.J., Jiang,J., Luu,D.A.T., Ng,S.L., Li,P.Y.Q., Wang,A.,
            Qi,R., Ong,G.J.X., Ng,T.Y., Haigh,J.J., Tiang,Z., Richards,A.M. and
            Foo,R.S.Y.
  TITLE     Asparagine Synthetase Marks a Distinct Dependency Threshold for
            Cardiomyocyte Dedifferentiation
  JOURNAL   Circulation 149 (23), 1833-1851 (2024)
   PUBMED   38586957
  REMARK    GeneRIF: Asparagine Synthetase Marks a Distinct Dependency
            Threshold for Cardiomyocyte Dedifferentiation.
REFERENCE   4  (bases 1 to 1869)
  AUTHORS   Clerici,S., Podrini,C., Stefanoni,D., Distefano,G., Cassina,L.,
            Steidl,M.E., Tronci,L., Canu,T., Chiaravalli,M., Spies,D.,
            Bell,T.A. 3rd, Costa,A.S., Esposito,A., D'Alessandro,A., Frezza,C.,
            Bachi,A. and Boletta,A.
  TITLE     Inhibition of asparagine synthetase effectively retards polycystic
            kidney disease progression
  JOURNAL   EMBO Mol Med 16 (6), 1379-1403 (2024)
   PUBMED   38684863
  REMARK    GeneRIF: Inhibition of asparagine synthetase effectively retards
            polycystic kidney disease progression.
REFERENCE   5  (bases 1 to 1869)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 1869)
  AUTHORS   Ruzzo,E.K., Capo-Chichi,J.M., Ben-Zeev,B., Chitayat,D., Mao,H.,
            Pappas,A.L., Hitomi,Y., Lu,Y.F., Yao,X., Hamdan,F.F., Pelak,K.,
            Reznik-Wolf,H., Bar-Joseph,I., Oz-Levi,D., Lev,D., Lerman-Sagie,T.,
            Leshinsky-Silver,E., Anikster,Y., Ben-Asher,E., Olender,T.,
            Colleaux,L., Decarie,J.C., Blaser,S., Banwell,B., Joshi,R.B.,
            He,X.P., Patry,L., Silver,R.J., Dobrzeniecka,S., Islam,M.S.,
            Hasnat,A., Samuels,M.E., Aryal,D.K., Rodriguiz,R.M., Jiang,Y.H.,
            Wetsel,W.C., McNamara,J.O., Rouleau,G.A., Silver,D.L., Lancet,D.,
            Pras,E., Mitchell,G.A., Michaud,J.L. and Goldstein,D.B.
  TITLE     Deficiency of asparagine synthetase causes congenital microcephaly
            and a progressive form of encephalopathy
  JOURNAL   Neuron 80 (2), 429-441 (2013)
   PUBMED   24139043
REFERENCE   7  (bases 1 to 1869)
  AUTHORS   Iida,S., Kamiya,H., Nakaya,A., Hayashi,Y., Sawada,A., Kaji,N.,
            Baba,Y. and Harashima,H.
  TITLE     Reduced plasma glucose by asparagine synthetase knockdown in the
            mouse liver
  JOURNAL   Biol Pharm Bull 36 (12), 2009-2011 (2013)
   PUBMED   24292060
  REMARK    GeneRIF: Asns is involved in the regulation of plasma glucose
            levels.
REFERENCE   8  (bases 1 to 1869)
  AUTHORS   Seo,J., Fortuno,E.S. 3rd, Suh,J.M., Stenesen,D., Tang,W.,
            Parks,E.J., Adams,C.M., Townes,T. and Graff,J.M.
  TITLE     Atf4 regulates obesity, glucose homeostasis, and energy expenditure
  JOURNAL   Diabetes 58 (11), 2565-2573 (2009)
   PUBMED   19690063
REFERENCE   9  (bases 1 to 1869)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
REFERENCE   10 (bases 1 to 1869)
  AUTHORS   Van Dyck,F., Braem,C.V., Chen,Z., Declercq,J., Deckers,R.,
            Kim,B.M., Ito,S., Wu,M.K., Cohen,D.E., Dewerchin,M., Derua,R.,
            Waelkens,E., Fiette,L., Roebroek,A., Schuit,F., Van de Ven,W.J. and
            Shivdasani,R.A.
  TITLE     Loss of the PlagL2 transcription factor affects lacteal uptake of
            chylomicrons
  JOURNAL   Cell Metab 6 (5), 406-413 (2007)
   PUBMED   17983586
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129291.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.3484054.1,
                                           SRR17253014.1322618.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164133
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC129291.4         86837-86850
            15-286              AC129291.4         90224-90495
            287-524             AC129291.4         94341-94578
            525-710             AC129291.4         97291-97476
            711-812             AC129291.4         97712-97813
            813-940             AC129291.4         98370-98497
            941-1067            AC129291.4         99536-99662
            1068-1174           AC129291.4         101701-101807
            1175-1275           AC129291.4         102416-102516
            1276-1357           AC129291.4         103431-103512
            1358-1513           AC129291.4         103599-103754
            1514-1869           AC129291.4         104223-104578
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 3.31 cM"
     gene            1..1869
                     /gene="Asns"
                     /note="asparagine synthetase"
                     /db_xref="GeneID:27053"
                     /db_xref="MGI:MGI:1350929"
     exon            1..14
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            15..286
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..1723
                     /gene="Asns"
                     /EC_number="6.3.5.4"
                     /note="isoform a is encoded by transcript variant 4;
                     glutamine-dependent asparagine synthetase"
                     /codon_start=1
                     /product="asparagine synthetase [glutamine-hydrolyzing]
                     isoform a"
                     /protein_id="NP_001397536.1"
                     /db_xref="GeneID:27053"
                     /db_xref="MGI:MGI:1350929"
                     /translation="MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCC
                     FGFHRLAVVDPLFGMQPIRVRKYPYLWLCYNGEIYNHKALQQRFEFEYQTNVDGEIIL
                     HLYDKGGIEKTICMLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSE
                     AKGLVSLKHSTTPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDS
                     VEKLFPGFDLETVKNNLRILFDNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKE
                     AQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIFSLETY
                     DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESER
                     LLKELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPDMRIPKNGIEKHLLRE
                     TFEDCNLLPKEILWRPKEAFSDGITSVKNSWFKILQDYVEHQVDDEMMSAASQKFPFN
                     TPKTKEGYFYRQIFERHYPGRADWLTHYWMPKWINATDPSARTLTHYKSAAKA"
     exon            287..524
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            525..710
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            711..812
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            813..940
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1067
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1174
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1275
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1357
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1513
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1869
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1848..1853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asns"
                     /note="hexamer: AATAAA"
     polyA_site      1869
                     /gene="Asns"
                     /note="major polyA site"
ORIGIN      
        1 aggaaagctt tcatagtgcc tgcagtccgc ctgtagcatg tgtggcatct gggccctctt
       61 cggcagcgat gactgccttt ccgtgcagtg tctgagtgcg atgaagatcg cgcacagggg
      121 gccagatgca tttcgctttg agaatgtcaa tggatacacc aactgctgct ttggctttca
      181 ccgcttggct gtggttgacc cgctgtttgg aatgcagccg ataagagtga ggaaataccc
      241 ttatttgtgg ctctgttaca atggtgaaat ctacaaccac aaggcgctac agcaacgttt
      301 tgaatttgaa tatcagacca atgtggatgg tgagattatc ctccacctct atgacaaagg
      361 aggcatcgag aaaaccatct gtatgctgga cggggtgttt gcattcatct tactggacac
      421 tgccaataag aaagtatttc tgggcagaga cacctatgga gtcaggccct tgtttaaagc
      481 catgacagaa gatgggtttc tggctgtgtg ttcagaagct aaaggccttg tttccttaaa
      541 acactccacc actcccttct taaaagtgga gcccttcctt cctggacact atgaagtttt
      601 ggatttaaaa ccaaatggca aagttgcgtc tgtggaaatg gtcaaatacc atcactgtac
      661 ggatgaacca ttgcatgcca tctatgacag cgtggagaaa ctcttcccag gctttgacct
      721 agagaccgtg aagaacaatc tgcgtatcct ttttgacaac gctatcaaga aacgcttgat
      781 gacagaccgg aggattggct gccttttatc agggggcctg gactcgagct tggttgctgc
      841 ctctctgctg aagcaactca aggaggccca agttcagtat cctctccaga catttgctat
      901 tggcatggag gacagccccg atctcctggc cgctagaaag gtggcaaatt atattggaag
      961 cgagcatcat gaagtccttt ttaactctga agaaggcatt caggccctgg atgaagtcat
     1021 attttccttg gaaacttatg atattacgac agttcgggca tctgtgggca tgtatttaat
     1081 ttccaagtat attcggaaga acacagacag cgtggtgatc ttctccggag aggggtcaga
     1141 tgaacttaca cagggctata tatatttcca caaggctcct tcccctgaga aggccgagga
     1201 ggagagtgag agactgctga aggaactcta cctgtttgat gttctccggg ccgaccgcac
     1261 tactgctgca cacggtctcg aactgagagt cccctttctg gatcatcggt tttcttccta
     1321 ttacctgtct ctgccgccag atatgagaat tccaaaaaat ggcatagaaa aacatctcct
     1381 gagagagact tttgaggact gcaacctgct acccaaagag attctctggc gacccaaaga
     1441 agccttcagt gatgggatca cctcagtcaa gaactcctgg ttcaagattt tgcaggacta
     1501 tgttgaacat caggttgatg atgagatgat gtctgcagcc tcccagaagt ttcccttcaa
     1561 tactcccaaa actaaggaag gctacttcta ccgtcagatc tttgaacgcc attacccagg
     1621 ccgggctgat tggctgactc attattggat gcctaagtgg atcaatgcta ctgacccttc
     1681 tgcccgcact ctgacccatt ataagtcagc tgccaaagct taggcactct ctacactctt
     1741 gtgtaaaagt aaatgtttct tccggctctg aaggtcgaga cagcgacaca atcagaaaga
     1801 atgagactca gccatcagtc acccaggctt acttaggcat gaaaagaaat aaaagtttca
     1861 catctgaaa
//
